##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629446 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82495718457183 33.0 31.0 34.0 30.0 34.0 2 31.956280602307427 33.0 31.0 34.0 30.0 34.0 3 31.97227085405261 34.0 31.0 34.0 30.0 34.0 4 35.56581025219002 37.0 35.0 37.0 33.0 37.0 5 35.563948297391676 37.0 35.0 37.0 33.0 37.0 6 35.61621330503331 37.0 35.0 37.0 33.0 37.0 7 35.57260193884781 37.0 35.0 37.0 33.0 37.0 8 35.54949113982772 37.0 35.0 37.0 33.0 37.0 9 37.18715028771332 39.0 37.0 39.0 34.0 39.0 10-11 37.0824423381831 39.0 37.0 39.0 33.0 39.0 12-13 36.95788359922852 39.0 37.0 39.0 33.0 39.0 14-15 38.11494870092113 40.0 38.0 41.0 33.0 41.0 16-17 38.0537091346994 40.0 38.0 41.0 33.0 41.0 18-19 38.01280173358795 40.0 38.0 41.0 33.0 41.0 20-21 37.936309866136256 40.0 38.0 41.0 33.0 41.0 22-23 37.75375091747346 40.0 37.5 41.0 32.5 41.0 24-25 37.57856750221623 40.0 37.0 41.0 32.0 41.0 26-27 37.51622299609498 40.0 37.0 41.0 32.0 41.0 28-29 37.56505879773643 40.0 37.0 41.0 32.0 41.0 30-31 37.39160547529097 40.0 37.0 41.0 32.0 41.0 32-33 37.154224508536075 39.5 36.5 41.0 31.5 41.0 34-35 37.165136485099595 40.0 36.5 41.0 31.0 41.0 36-37 37.070568404597054 40.0 36.0 41.0 31.0 41.0 38-39 36.939292647820466 40.0 36.0 41.0 31.0 41.0 40-41 36.71523371345596 39.5 36.0 41.0 30.5 41.0 42-43 36.46637201602679 39.0 36.0 41.0 30.0 41.0 44-45 36.238655103058875 39.0 35.0 41.0 30.0 41.0 46-47 35.884803462092066 39.0 35.0 41.0 28.5 41.0 48-49 35.52417284405652 39.0 35.0 40.0 27.0 41.0 50-51 34.57354006539084 38.0 34.0 39.5 25.0 40.5 52-53 34.67155641627717 38.0 34.5 39.5 26.0 40.5 54-55 35.02394168840536 39.0 35.0 40.0 26.0 41.0 56-57 34.80870400320282 38.0 34.5 40.0 25.5 41.0 58-59 34.56671342736311 38.0 34.0 40.0 24.5 41.0 60-61 34.23529738849719 38.0 34.0 40.0 23.5 41.0 62-63 33.88546912681946 37.5 34.0 40.0 22.5 41.0 64-65 33.52099544678972 37.0 33.0 40.0 22.0 41.0 66-67 33.10313196048588 36.5 33.0 39.0 20.5 41.0 68-69 32.617454078666 36.0 32.0 39.0 18.5 40.0 70-71 32.15964435392392 35.0 31.5 38.5 17.5 40.0 72-73 31.705681662922633 35.0 31.0 38.0 13.5 40.0 74-75 31.252684106341132 35.0 31.0 37.0 9.5 39.0 76-77 30.763728739240538 35.0 30.5 36.5 7.0 39.0 78-79 30.233179653218862 34.0 30.0 36.0 4.5 38.5 80-81 29.685447997127632 34.0 29.0 35.5 2.0 37.5 82-83 29.15135849620142 34.0 29.0 35.0 2.0 37.0 84-85 28.691377814776803 34.0 29.0 35.0 2.0 36.5 86-87 28.239971339876654 34.0 27.5 35.0 2.0 36.0 88-89 27.75769962792678 33.5 26.5 35.0 2.0 36.0 90-91 27.268626697127317 33.0 26.0 35.0 2.0 35.0 92-93 26.68386644763808 33.0 25.0 35.0 2.0 35.0 94-95 26.049357689142518 32.0 24.5 34.5 2.0 35.0 96-97 25.30500233538699 32.0 20.5 34.0 2.0 35.0 98-99 24.35958445998545 31.5 9.5 34.0 2.0 35.0 100-101 21.969539881101795 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22220397307387785 1101 2 0.15855687120935258 1101 3 0.09715042402015328 1101 4 0.11862875510997384 1101 5 0.08041967736915723 1101 6 0.09093740013371843 1101 7 0.09603997835041866 1101 8 0.1756551784732565 1101 9 0.25628356293964316 1101 10-11 0.23533054078448146 1101 12-13 0.3599972346286364 1101 14-15 0.49874961412938035 1101 16-17 0.40975689437456 1101 18-19 0.11733353004530755 1101 20-21 0.4700925375845486 1101 22-23 0.3805243347541065 1101 24-25 0.37810557137819245 1101 26-27 0.4466036465969765 1101 28-29 0.26782754556272437 1101 30-31 0.46142008327191775 1101 32-33 0.4472423510607584 1101 34-35 0.6385758947853404 1101 36-37 0.5148782343155816 1101 38-39 0.561435498880634 1101 40-41 0.6275049016560672 1101 42-43 0.5229421044346623 1101 44-45 0.9081924503167542 1101 46-47 0.7790510699378075 1101 48-49 0.8957339276184584 1101 50-51 0.7584218075722831 1101 52-53 0.9994092015557428 1101 54-55 1.122082949635498 1101 56-57 1.0193508430936191 1101 58-59 1.0743498102165105 1101 60-61 0.9806575892960652 1101 62-63 1.2711402788861648 1101 64-65 0.9321534414741706 1101 66-67 1.1011969966703816 1101 68-69 1.2336246350162767 1101 70-71 1.1772181501914005 1101 72-73 1.2392406853047184 1101 74-75 1.322504993232318 1101 76-77 1.2134209032237635 1101 78-79 1.5544955289956484 1101 80-81 1.5467410942492776 1101 82-83 1.6403208160606333 1101 84-85 1.7990176547114807 1101 86-87 1.847564621092321 1101 88-89 1.9435662704635774 1101 90-91 2.025867885919798 1101 92-93 1.8438706167109196 1101 94-95 2.1898797337373175 1101 96-97 2.281249650746897 1101 98-99 2.3923596987767084 1101 100-101 2.704029519954485 1102 1 0.20265960998029087 1102 2 0.08595909777115551 1102 3 0.12911963917776248 1102 4 0.17284441419941032 1102 5 -0.011564641122397745 1102 6 -6.575355296192242E-4 1102 7 0.0058418060898191015 1102 8 0.05879313208950521 1102 9 0.3218783452097469 1102 10-11 0.10182680329930349 1102 12-13 0.13446788047315295 1102 14-15 0.30331630773819995 1102 16-17 0.522823933118147 1102 18-19 0.3408429607794119 1102 20-21 0.3732549456223566 1102 22-23 0.2568945493276402 1102 24-25 0.2720142375060206 1102 26-27 0.5365768912768871 1102 28-29 0.33430065159873834 1102 30-31 0.5491234030555106 1102 32-33 0.29343760884989223 1102 34-35 0.3790618268557324 1102 36-37 0.40923380573487833 1102 38-39 0.5586168981809223 1102 40-41 0.553297974181568 1102 42-43 0.5157687582792292 1102 44-45 0.6409556229608384 1102 46-47 0.6378533342579544 1102 48-49 0.7662656121711606 1102 50-51 1.031931533293374 1102 52-53 0.9134057443082 1102 54-55 0.7400091615895477 1102 56-57 0.7218254889617342 1102 58-59 0.4502856564456863 1102 60-61 0.5349388829910069 1102 62-63 0.7735392605910718 1102 64-65 0.817264858060696 1102 66-67 1.0447438612536502 1102 68-69 1.0462275117294197 1102 70-71 1.013955387579223 1102 72-73 1.0782847209627455 1102 74-75 1.112907886464729 1102 76-77 0.9772985662188276 1102 78-79 0.9465170158480767 1102 80-81 1.159051491360767 1102 82-83 1.034771520784556 1102 84-85 1.2771052835470478 1102 86-87 1.261759542596284 1102 88-89 1.3357367257464965 1102 90-91 1.03751913459973 1102 92-93 0.9781413203728881 1102 94-95 1.4089960707068592 1102 96-97 1.6327148511315563 1102 98-99 1.8144123362553977 1102 100-101 2.0529062094924804 1103 1 0.1443276869932575 1103 2 0.17951099448545804 1103 3 0.11889261371900162 1103 4 0.16091852588272104 1103 5 0.04216874701295836 1103 6 0.09025096926153253 1103 7 0.17579417668046915 1103 8 0.24221551871780633 1103 9 0.2483888260975391 1103 10-11 0.009329039705583853 1103 12-13 0.1182609867004416 1103 14-15 0.2911550930673741 1103 16-17 0.18029353008147098 1103 18-19 0.13087060356690472 1103 20-21 0.23529487051133913 1103 22-23 0.3168262079754811 1103 24-25 0.28546007617852354 1103 26-27 0.25061668721992447 1103 28-29 -0.02437700770994411 1103 30-31 -0.04903508763217701 1103 32-33 -0.24490043906964587 1103 34-35 -0.4437027742580355 1103 36-37 -0.34023872474875105 1103 38-39 -0.25575654540119785 1103 40-41 0.022097890366701733 1103 42-43 -0.05690356589088452 1103 44-45 -0.11127232123779862 1103 46-47 -0.1900368682677822 1103 48-49 -0.1316341807094048 1103 50-51 -0.20756702815339878 1103 52-53 -0.14593276298126767 1103 54-55 -0.06605774523894326 1103 56-57 -0.04997242050209394 1103 58-59 -0.08074760354963928 1103 60-61 -0.29329947359288866 1103 62-63 -0.2532186353334325 1103 64-65 -0.19301777533790698 1103 66-67 -0.20406459760049955 1103 68-69 -0.2462976528781482 1103 70-71 -0.18719142544108536 1103 72-73 -0.2223543673894035 1103 74-75 -0.20182499394596576 1103 76-77 -0.22284891570859244 1103 78-79 -0.16019784436645423 1103 80-81 -0.09517027955063995 1103 82-83 -0.09539860727489113 1103 84-85 0.1949530145013263 1103 86-87 0.3398395374384471 1103 88-89 0.39083774683722083 1103 90-91 0.4729994179584516 1103 92-93 0.5495668984133921 1103 94-95 0.9497196186546013 1103 96-97 1.0878231254318251 1103 98-99 1.2486845572687528 1103 100-101 1.6011102342178667 1104 1 0.287755958545187 1104 2 0.38749725468296603 1104 3 0.25860923029263816 1104 4 0.25651511001515814 1104 5 0.19577783054904785 1104 6 0.18432090867281659 1104 7 0.3905358397690648 1104 8 0.4501636127972901 1104 9 0.5826953954406093 1104 10-11 0.40487791619047897 1104 12-13 0.47806729365144207 1104 14-15 0.5156863654303265 1104 16-17 0.6733950042477801 1104 18-19 0.748293436447014 1104 20-21 0.7192412471155123 1104 22-23 0.5703446469812548 1104 24-25 0.5287595370821023 1104 26-27 0.49298107823180004 1104 28-29 0.5477903507176052 1104 30-31 0.7221638055504016 1104 32-33 0.6715586993302409 1104 34-35 0.6940005795895843 1104 36-37 0.8929324025829644 1104 38-39 0.7807541172580201 1104 40-41 0.9847046362280665 1104 42-43 1.1484921690454257 1104 44-45 1.1902018450710656 1104 46-47 0.9812574402098733 1104 48-49 0.9386057887577053 1104 50-51 0.8608028015303049 1104 52-53 1.1178285079504562 1104 54-55 0.8621040791014991 1104 56-57 0.8391025282121873 1104 58-59 1.0887719804396738 1104 60-61 1.0781665824158893 1104 62-63 1.0533173123789865 1104 64-65 1.1473142698889376 1104 66-67 1.385091454114594 1104 68-69 1.0086997232012394 1104 70-71 0.9886446510819766 1104 72-73 0.8037178619889609 1104 74-75 1.013221998086852 1104 76-77 1.0681200266128599 1104 78-79 1.3469535319991763 1104 80-81 1.1017859725365682 1104 82-83 1.3777706117509219 1104 84-85 1.3447454862345474 1104 86-87 1.7052419514454122 1104 88-89 1.6259362631293826 1104 90-91 1.4330108677938576 1104 92-93 1.4567421286027944 1104 94-95 1.3087530617155245 1104 96-97 1.8035141707063822 1104 98-99 2.036738562325759 1104 100-101 2.576688689444236 1105 1 0.26613705331006443 1105 2 0.1665718429212113 1105 3 0.15645462647590946 1105 4 0.08572475359468967 1105 5 0.06680060778939634 1105 6 -0.006294315519049576 1105 7 0.18151791195771239 1105 8 0.190618655072754 1105 9 0.2958931290235327 1105 10-11 0.36980624263494377 1105 12-13 0.2558252493532862 1105 14-15 0.18108455882958197 1105 16-17 0.21786904221403347 1105 18-19 0.3578063577882702 1105 20-21 0.12506684355758324 1105 22-23 0.3803688941262493 1105 24-25 0.436266101399859 1105 26-27 0.34845678711530326 1105 28-29 0.4101318962532474 1105 30-31 0.11546380429407321 1105 32-33 0.1666854000852993 1105 34-35 0.3565582136712706 1105 36-37 0.5353397797454207 1105 38-39 0.20409293656026506 1105 40-41 0.3083060282403238 1105 42-43 0.6242513056963048 1105 44-45 0.2996959214809749 1105 46-47 0.27695828320646854 1105 48-49 -0.028857809116594524 1105 50-51 -0.2195004762314241 1105 52-53 -0.10289337855279967 1105 54-55 0.010739077907985006 1105 56-57 -0.1587717635324779 1105 58-59 -0.12837565075245294 1105 60-61 0.060736277253631954 1105 62-63 0.2729386548918953 1105 64-65 0.3227191218305947 1105 66-67 0.0762544376441241 1105 68-69 0.22452569654194576 1105 70-71 0.3007255084007241 1105 72-73 0.3864697244963402 1105 74-75 0.3799418493375306 1105 76-77 0.5227992230649896 1105 78-79 0.5227918173616892 1105 80-81 0.35716117511958245 1105 82-83 0.223410522802844 1105 84-85 0.5279961112581013 1105 86-87 0.5967956532180914 1105 88-89 0.8620042053885122 1105 90-91 1.0103454407048957 1105 92-93 1.4819327764915329 1105 94-95 1.3380280343903763 1105 96-97 1.4783071905438128 1105 98-99 1.3214540666789425 1105 100-101 1.7910824480029781 1106 1 0.07551016623886397 1106 2 0.10739974848612022 1106 3 0.11447808993575492 1106 4 -0.007932103800044388 1106 5 0.20547914934775235 1106 6 0.08831838884975696 1106 7 0.07932947447688576 1106 8 -0.012401950533806883 1106 9 0.1223100268480124 1106 10-11 0.04311486172669987 1106 12-13 0.12464978362007884 1106 14-15 0.19721770572969888 1106 16-17 -0.08631795503790585 1106 18-19 0.2712279347420221 1106 20-21 0.23878357055671273 1106 22-23 0.2457462437821789 1106 24-25 0.32526191311091424 1106 26-27 0.4400291876797695 1106 28-29 0.35856944166405214 1106 30-31 0.6463431512180904 1106 32-33 0.6496513028278699 1106 34-35 0.3800265173335191 1106 36-37 0.38254634925583986 1106 38-39 0.5922330068925135 1106 40-41 0.492676315607973 1106 42-43 0.5424747220096435 1106 44-45 0.7841843980352721 1106 46-47 0.9781172476625244 1106 48-49 0.9962864559630233 1106 50-51 0.8081302826658643 1106 52-53 0.8415189531648437 1106 54-55 0.9371194856283935 1106 56-57 0.7320114174358352 1106 58-59 0.8873586883730127 1106 60-61 1.0411607895617792 1106 62-63 1.2771447044990651 1106 64-65 1.1567375864691058 1106 66-67 1.2833809872625892 1106 68-69 1.110085625886768 1106 70-71 1.1723739892175828 1106 72-73 1.0801643636802574 1106 74-75 1.4037863562851847 1106 76-77 1.5856122548759686 1106 78-79 1.6809957938142688 1106 80-81 1.424067234091467 1106 82-83 1.0531954895258089 1106 84-85 0.9352208289441784 1106 86-87 1.1966348384231331 1106 88-89 1.4360032301662606 1106 90-91 1.4836237051967949 1106 92-93 1.6611121043631911 1106 94-95 1.451727000719373 1106 96-97 1.4585928467036986 1106 98-99 1.9120415573545912 1106 100-101 2.646207047176947 1107 1 0.039080254687000604 1107 2 0.06029530821094653 1107 3 -0.04659758235943201 1107 4 0.2055870599630154 1107 5 0.2491528739970903 1107 6 -0.013913363138094326 1107 7 0.1211163055320128 1107 8 0.12475520125749284 1107 9 -0.012563554854843062 1107 10-11 0.36428702289885706 1107 12-13 0.29616948343246463 1107 14-15 0.3233679782214338 1107 16-17 0.4209097765800678 1107 18-19 0.4247828351261873 1107 20-21 0.45531354464765883 1107 22-23 0.44726505017903406 1107 24-25 0.2739941564773076 1107 26-27 0.3002525415616546 1107 28-29 0.26858284289696144 1107 30-31 0.28870534187296215 1107 32-33 0.29857295028610054 1107 34-35 0.5648313060407517 1107 36-37 0.5457930212829964 1107 38-39 0.07953757224586155 1107 40-41 0.1938252479764131 1107 42-43 0.375645453716956 1107 44-45 0.4144979868854506 1107 46-47 0.35569609158282844 1107 48-49 0.2614003664427855 1107 50-51 -0.0932813138676849 1107 52-53 -0.02111483581040119 1107 54-55 0.027032824322212434 1107 56-57 0.3034542835128491 1107 58-59 0.014229053779956757 1107 60-61 0.15024861230813968 1107 62-63 0.317126836188514 1107 64-65 0.06294861121671147 1107 66-67 0.14777480482713656 1107 68-69 0.1975492192040207 1107 70-71 0.42870026456824206 1107 72-73 0.5273188352249782 1107 74-75 0.5761208510587004 1107 76-77 0.42846761090205376 1107 78-79 0.713508971693301 1107 80-81 0.8633918119526278 1107 82-83 1.0433875738642335 1107 84-85 1.1965216419523053 1107 86-87 1.0148908913133319 1107 88-89 1.0863186058474916 1107 90-91 1.1640918549332362 1107 92-93 1.3109861327738024 1107 94-95 1.513174333874023 1107 96-97 1.8949566455065217 1107 98-99 2.4714483294442893 1107 100-101 2.8950411399134772 1108 1 0.060752862309705336 1108 2 0.06595109835809865 1108 3 -0.03973897711311025 1108 4 0.12286701609134099 1108 5 -0.19395835897979197 1108 6 0.05951319750535333 1108 7 -0.033132849024241295 1108 8 -0.13330164912181885 1108 9 -0.023012325533940725 1108 10-11 0.08135493259632653 1108 12-13 -0.08778445130841561 1108 14-15 -0.06028068277847609 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-1.0372154011464367 2316 86-87 -0.9704243608104335 2316 88-89 -1.0303254593072104 2316 90-91 -1.0141608143832634 2316 92-93 -1.3049970329965923 2316 94-95 -1.2416584928736967 2316 96-97 -1.1543567361472817 2316 98-99 -0.9397807251890242 2316 100-101 -1.0472782428314389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2430.0 3 469.0 4 431.0 5 543.0 6 562.0 7 642.0 8 803.0 9 969.0 10 1253.0 11 1653.0 12 2102.0 13 2766.0 14 3264.0 15 4098.0 16 5175.0 17 6877.0 18 6091.0 19 4230.0 20 4097.0 21 4233.0 22 4567.0 23 5068.0 24 5676.0 25 6421.0 26 7618.0 27 8710.0 28 10375.0 29 12675.0 30 15475.0 31 19226.0 32 24648.0 33 32208.0 34 44516.0 35 62899.0 36 91275.0 37 122824.0 38 92257.0 39 10320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09459065515247 19.365285696307588 15.720664535234736 21.8194591133052 2 14.889214875835608 20.353531250945707 40.875736855266574 23.88151701795211 3 19.276081943612805 24.72051647917649 29.975921419744434 26.02748015746627 4 12.40911711034608 17.132957328433168 38.19151409381439 32.266411467406364 5 13.448794173563853 37.6352235706984 34.16327778432378 14.752704471413969 6 30.84652421312323 36.92746010902869 17.517647526958736 14.708368150889338 7 28.077354010300287 33.431370676648655 20.551555547067473 17.93971976598359 8 26.222984824705776 34.57608180078885 19.681245602921095 19.519687771584284 9 25.91155305050132 17.796991918706357 21.016457060268273 35.274997970524055 10-11 24.732019776574372 27.266331114358394 26.84935802539883 21.15229108366841 12-13 26.08286994259446 24.590787577228472 27.467749675885138 21.858592804291927 14-15 22.63321626648267 25.94530885036533 26.559531796263887 24.861943086888115 16-17 21.421806831765274 29.484967474226885 27.0236092386884 22.06961645531944 18-19 21.455407841280326 27.69508696396099 29.72333085300683 21.126174341751852 20-21 22.24484761677409 26.488819925200925 29.978515158749104 21.287817299275883 22-23 21.94952914824967 26.535111973996727 30.00648490370117 21.508873974052428 24-25 21.94706249776211 26.782095300235127 29.961607778701666 21.309234423301092 26-27 21.451546418004753 26.905031329427697 30.092165971960284 21.551256280607266 28-29 21.204205982350917 27.556315340438807 29.982984426532198 21.256494250678077 30-31 21.44424345495364 26.855281995860132 29.854181372810395 21.846293176375838 32-33 21.231441430328015 27.05860349623357 29.760086590077954 21.94986848336046 34-35 22.098014623727106 27.0203917274397 29.483808535494532 21.39778511333866 36-37 21.972110047942074 26.54924506156876 29.81296624162008 21.665678648869086 38-39 21.471835636930763 27.230272214397683 29.74011919823669 21.55777295043486 40-41 21.67586225554242 27.18462206845847 29.603775416506867 21.53574025949225 42-43 21.61476300177853 27.065662413311532 29.17905073389275 22.140523851017186 44-45 21.73024604467333 27.12093277516207 29.222907514225486 21.925913665939113 46-47 22.156857440232734 27.595988885246214 28.727596227604185 21.519557446916863 48-49 21.97788293041622 27.611082727326263 28.53390058459986 21.877133757657656 50-51 21.694052741074653 27.84441908254661 28.689479640709987 21.772048535668752 52-53 22.272582906076792 27.936488225868462 28.125619392631044 21.665309475423694 54-55 21.70033017587894 27.65694495397512 28.502319348274675 22.14040552187127 56-57 22.0882246470497 27.874580815574767 28.250026851671368 21.78716768570417 58-59 22.415826081144946 27.471830638526747 28.123851305871288 21.98849197445702 60-61 21.843695347397603 27.666808295976615 28.879966772809084 21.609529583816702 62-63 21.70852599925679 27.879405653973993 28.858868680020596 21.553199666748625 64-65 21.380986139058162 28.019534690545584 28.48944297647833 22.110036193917924 66-67 21.495993386362315 27.90243770473672 28.35167493238765 22.24989397651331 68-69 21.586535439398894 27.774634752628007 28.590229886429373 22.048599921543726 70-71 21.777592474862768 27.90386067484033 28.177412334967904 22.141134515328996 72-73 21.81409641295924 27.724812659432434 28.410521307254445 22.050569620353883 74-75 21.569649381898735 28.49623473843686 28.0229512266541 21.9111646530103 76-77 21.78774550724038 27.885150238772976 28.150780697307802 22.176323556678838 78-79 21.879503881644656 28.012940141895896 28.04930942139421 22.05824655506524 80-81 22.02301945768042 28.255254337633566 27.871941534651732 21.849784670034282 82-83 21.976808217390335 28.177251936096866 28.102052119441183 21.743887727071616 84-85 21.806079943982716 28.390577320161846 27.865574435749213 21.937768300106224 86-87 21.969710834189378 28.370650398288287 27.760029218151853 21.89960954937048 88-89 21.763286196615102 28.430435609481663 27.500539110797412 22.30573908310582 90-91 21.873602492874205 28.13332038940019 27.865556034764012 22.127521082961593 92-93 22.285136823343123 28.07229874356465 27.84345086056671 21.79911357252552 94-95 21.805295622947064 27.901484480431847 27.9552746161485 22.33794528047259 96-97 21.725967647356022 27.939263404880666 28.11096426982974 22.223804677933575 98-99 21.74757837750099 28.22139048155557 27.39333642053402 22.63769472040942 100-101 21.841271710426334 27.451113001232468 28.177068124729978 22.53054716361122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 865.0 1 499.0 2 140.5 3 150.0 4 135.0 5 130.0 6 221.5 7 483.0 8 813.5 9 981.0 10 896.0 11 770.5 12 780.5 13 854.5 14 927.5 15 942.0 16 988.0 17 1087.0 18 1145.5 19 1188.0 20 1287.5 21 1477.0 22 1749.0 23 2122.5 24 2504.5 25 3014.5 26 3882.0 27 4790.5 28 5557.5 29 6558.0 30 7777.5 31 9187.0 32 10827.5 33 12265.5 34 13571.0 35 14562.0 36 15934.5 37 17491.0 38 18127.5 39 18574.5 40 19225.0 41 20451.5 42 21484.5 43 22274.5 44 23208.0 45 24142.0 46 24261.0 47 25410.0 48 28453.0 49 27615.0 50 23888.0 51 21398.5 52 19758.5 53 19061.5 54 18649.0 55 17091.5 56 15176.0 57 13617.5 58 12160.5 59 10426.5 60 8360.0 61 6782.0 62 5530.5 63 4191.5 64 3081.5 65 2363.0 66 1763.5 67 1295.5 68 924.0 69 652.5 70 479.0 71 349.0 72 283.5 73 232.0 74 168.0 75 123.0 76 89.5 77 62.0 78 40.0 79 25.0 80 17.5 81 14.0 82 10.0 83 5.0 84 3.5 85 5.0 86 3.0 87 1.5 88 2.0 89 3.0 90 2.5 91 1.0 92 0.5 93 0.0 94 0.0 95 1.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2699198978149039 2 0.25562161011429096 3 0.23862253473689563 4 0.22877260320980672 5 0.2243242470362827 6 0.1860366099713081 7 0.17698102776091992 8 0.18873739764809053 9 0.19143818532487297 10-11 0.1950921921816963 12-13 0.19008779148648175 14-15 0.18627491476631833 16-17 0.18667208942466867 18-19 0.18563943531295773 20-21 0.17412137022079732 22-23 0.16895809966224268 24-25 0.16895809966224268 26-27 0.1784108565309812 28-29 0.19032609628149197 30-31 0.18516282572293732 32-33 0.19040553121316203 34-35 0.18301808256784538 36-37 0.17213549692904553 38-39 0.1717383222706952 40-41 0.1691964044572529 42-43 0.17769594214595055 44-45 0.1722943667923857 46-47 0.17348589076743676 48-49 0.18317695243118554 50-51 0.19175592505155326 52-53 0.20819895590725815 54-55 0.1818265585927943 56-57 0.1578372092284326 58-59 0.16125291129024572 60-61 0.16601900719045 62-63 0.1734064558357667 64-65 0.16292104485531722 66-67 0.16641618184880036 68-69 0.17007018870562368 70-71 0.16609844212212008 72-73 0.16601900719045 74-75 0.16999075377395362 76-77 0.17944351064269212 78-79 0.18563943531295773 80-81 0.17602780858087905 82-83 0.1779342469409608 84-85 0.16975244897894337 86-87 0.16991131884228355 88-89 0.17420080515246741 90-91 0.1739625003574572 92-93 0.1717383222706952 94-95 0.17126171268067475 96-97 0.1632387845819975 98-99 0.16601900719045 100-101 0.16403313389869822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 629446.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.34411279045285 #Duplication Level Percentage of deduplicated Percentage of total 1 84.43925356303312 55.17608108764486 2 9.49002073935915 12.402339711528422 3 2.532472691204802 4.964465435184849 4 1.082790592709138 2.830159624737089 5 0.5848358718402417 1.9107790586715794 6 0.371339932905963 1.4558927075643868 7 0.25254399673294514 1.1551584384948526 8 0.18445984466713541 0.9642691916190969 9 0.13696081957088932 0.8054624917721751 >10 0.8422697927699532 10.132081503260078 >50 0.05163364011127404 2.2996990281418412 >100 0.028252308147807735 3.484396145792026 >500 0.001948435044660031 0.8068826372103752 >1k 0.0012177719029125194 1.6123329383783653 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3170 0.503617466788255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.37906349392958255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1935 0.30741318556317776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1450 0.2303613018432082 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1190 0.1890551373747708 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 796 0.1264604112187543 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 780 0.12391849340531197 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 659 0.10469523994115461 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 648 0.10294767144441302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5886986334014356E-4 0.0 14-15 0.0 0.0 0.0 1.5886986334014356E-4 0.0 16-17 0.0 0.0 0.0 1.5886986334014356E-4 0.0 18-19 0.0 0.0 0.0 2.3830479501021532E-4 0.0 20-21 0.0 0.0 0.0 3.177397266802871E-4 0.0 22-23 0.0 0.0 0.0 3.177397266802871E-4 0.0 24-25 0.0 0.0 0.0 3.9717465835035885E-4 0.0 26-27 0.0 0.0 0.0 7.943493167007178E-4 0.0 28-29 0.0 0.0 0.0 0.0015092637017313638 0.0 30-31 1.5886986334014356E-4 0.0 0.0 0.0034157020618130864 0.0 32-33 1.5886986334014356E-4 0.0 0.0 0.00579875001191524 0.0 34-35 1.5886986334014356E-4 0.0 0.0 0.008102363030347321 0.0 36-37 1.5886986334014356E-4 0.0 0.0 0.010564845912119546 0.0 38-39 1.5886986334014356E-4 0.0 0.0 0.01342450345224213 0.0 40-41 1.5886986334014356E-4 0.0 0.0 0.018190599352446435 0.0 42-43 1.5886986334014356E-4 0.0 0.0 0.028358270606215624 0.0 44-45 3.177397266802871E-4 0.0 0.0 0.042577123375158475 0.0 46-47 3.177397266802871E-4 0.0 0.0 0.055683887100720315 0.0 48-49 3.177397266802871E-4 0.0 0.0 0.08253289400520458 0.0 50-51 3.177397266802871E-4 0.0 0.0 0.11057342488473992 0.0 52-53 3.177397266802871E-4 0.0 0.0 0.13813734617425483 0.0 54-55 3.177397266802871E-4 0.0 0.0 0.1863543496979884 0.0 56-57 4.7660959002043064E-4 0.0 0.0 0.2402906683019671 0.0 58-59 4.7660959002043064E-4 0.0 0.0 0.29486246635930646 0.0 60-61 4.7660959002043064E-4 0.0 0.0 0.3570600178569726 0.0 62-63 4.7660959002043064E-4 0.0 0.0 0.43808364816044587 0.0 64-65 4.7660959002043064E-4 0.0 0.0 0.4929731859444655 0.0 66-67 4.7660959002043064E-4 0.0 0.0 0.5747911655646394 0.0 68-69 4.7660959002043064E-4 0.0 0.0 0.6879065082628215 0.0 70-71 4.7660959002043064E-4 0.0 0.0 0.7811631180434858 0.0 72-73 4.7660959002043064E-4 0.0 0.0 0.9217629470995129 0.0 74-75 4.7660959002043064E-4 0.0 0.0 1.0943750536185788 0.0 76-77 4.7660959002043064E-4 0.0 0.0 1.2356103621279666 0.0 78-79 4.7660959002043064E-4 0.0 0.0 1.3750981021406126 0.0 80-81 4.7660959002043064E-4 0.0 0.0 1.578213222420986 0.0 82-83 4.7660959002043064E-4 0.0 0.0 1.7902250550484076 1.5886986334014356E-4 84-85 4.7660959002043064E-4 0.0 0.0 2.0158996959230815 1.5886986334014356E-4 86-87 4.7660959002043064E-4 0.0 0.0 2.2892352957997986 1.5886986334014356E-4 88-89 4.7660959002043064E-4 0.0 0.0 2.617222128665525 1.5886986334014356E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTAAC 70 0.0083475495 27.170248 1 GTATAGG 330 0.0 24.494394 1 GAGTACT 2575 0.0 23.797392 12-13 GGTATCA 3980 0.0 23.176634 1 GTACTTT 2660 0.0 22.858368 14-15 GTACATG 2620 0.0 22.685083 1 CTAACAC 235 4.618414E-9 22.242313 3 GTATCAA 5145 0.0 22.179796 1 GACGTAT 155 3.8987913E-5 21.45447 8 TAAGGTG 315 1.4551915E-11 21.118963 5 ACACGGT 135 3.789894E-4 21.113922 8 TACATGG 2700 0.0 20.947971 2 AGAGTAC 4080 0.0 20.841406 10-11 AGTACTT 2685 0.0 20.787893 12-13 GTAAGGT 275 1.2478267E-9 20.736631 4 CCACTCG 140 4.8336334E-4 20.366335 4 CGCGATC 70 4.8503326E-4 20.358234 22-23 ACATGGG 2670 0.0 20.288443 3 GTGTAAG 235 1.11785994E-7 20.233166 1 GTATACC 120 0.0047334363 19.81164 1 >>END_MODULE