FastQCFastQC Report
Thu 26 May 2016
SRR937609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629446
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.688383117852842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.576538734061381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.33569202123772335No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19590.31122606228334126No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12520.19890506890185974No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.163794829103688No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9920.15759890443342242No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7620.12105883586518938No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC6910.10977907556803919No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6890.1094613358413589No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6760.10739602761793705No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA6640.10548958925785533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGC702.0508998E-433.9324578
GCCGTAT350.00839205727.14273392-93
CTTACGG700.00839055127.1416533
GTATCAA56850.025.5742761
GGTATCA41750.024.2401621
GAGTACT33950.023.92763112-13
CCGGGTG1000.001651715523.752729
GGCCTAA1401.7784325E-523.7489476
GTACTTT35350.022.90916814-15
TAAGGTG3750.022.798995
TCAACGC65550.022.0280084
TATCAAC65850.021.9997842
CAACGCA66150.021.9000115
ATCAACG66250.021.795263
AACGCAG66950.021.6383236
GCGCTTT1553.8993607E-521.4540717
GTAAGGT3101.0913936E-1121.4506634
AGAGTAC48550.021.13527310-11
AGTACTT35000.020.76666612-13
ACTTTTT40250.020.12022616-17
Produced by FastQC (version 0.11.5)