##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937609_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629446 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23553251589493 33.0 31.0 34.0 31.0 34.0 2 32.44964778551297 34.0 31.0 34.0 31.0 34.0 3 32.50432443768012 34.0 31.0 34.0 31.0 34.0 4 36.11014606495235 37.0 35.0 37.0 35.0 37.0 5 36.02943064218376 37.0 35.0 37.0 35.0 37.0 6 36.08232159708696 37.0 35.0 37.0 35.0 37.0 7 36.04255964769019 37.0 35.0 37.0 35.0 37.0 8 36.01176749077761 37.0 35.0 37.0 35.0 37.0 9 37.700433396987194 39.0 37.0 39.0 35.0 39.0 10-11 37.613831845781846 39.0 37.0 39.0 35.0 39.0 12-13 37.51631275756777 39.0 37.0 39.0 35.0 39.0 14-15 38.7146554271534 40.0 38.0 41.0 34.5 41.0 16-17 38.67950705858803 40.0 38.0 41.0 34.5 41.0 18-19 38.67350813254831 40.0 38.0 41.0 34.0 41.0 20-21 38.58487225274288 40.0 38.0 41.0 34.0 41.0 22-23 38.539852505218875 40.0 38.0 41.0 34.0 41.0 24-25 38.53943706052624 40.0 38.0 41.0 34.0 41.0 26-27 38.36532522249725 40.0 38.0 41.0 34.0 41.0 28-29 38.32908780101867 40.0 38.0 41.0 34.0 41.0 30-31 38.326773067109805 40.0 38.0 41.0 34.0 41.0 32-33 38.168201084763425 40.0 37.5 41.0 34.0 41.0 34-35 38.006191952923686 40.0 37.0 41.0 33.0 41.0 36-37 37.815896041916226 40.0 37.0 41.0 33.0 41.0 38-39 37.6564423318283 40.0 37.0 41.0 33.0 41.0 40-41 37.476198117074375 40.0 37.0 41.0 33.0 41.0 42-43 37.246342815745905 40.0 36.5 41.0 32.5 41.0 44-45 36.913204627561385 40.0 36.0 41.0 31.0 41.0 46-47 36.527946003310845 39.5 36.0 41.0 30.5 41.0 48-49 36.13676391620568 39.0 35.5 41.0 30.0 41.0 50-51 35.87087931291961 39.0 35.0 40.5 29.0 41.0 52-53 35.60455861185868 39.0 35.0 40.0 28.0 41.0 54-55 35.37572404940217 39.0 35.0 40.0 27.5 41.0 56-57 35.100729848152184 38.0 35.0 40.0 27.0 41.0 58-59 34.82627024399233 38.0 34.0 40.0 26.0 41.0 60-61 34.4513850274686 38.0 34.0 40.0 26.0 41.0 62-63 34.05905113385421 37.0 34.0 40.0 25.0 41.0 64-65 33.667204176370966 37.0 33.0 39.5 23.5 41.0 66-67 33.23626331726629 36.0 33.0 39.0 22.5 40.0 68-69 32.75307413185563 36.0 32.0 39.0 22.0 40.0 70-71 32.30955872306758 35.0 32.0 38.0 20.5 40.0 72-73 31.83009980204815 35.0 31.0 37.5 19.0 39.5 74-75 31.350115816130376 35.0 31.0 37.0 18.0 39.0 76-77 30.102861087368893 33.5 29.5 35.5 17.5 38.0 78-79 30.718280837434826 34.0 31.0 36.0 17.0 38.0 80-81 30.533113245615986 34.0 31.0 36.0 13.5 37.0 82-83 30.120544097507967 34.0 30.0 35.0 8.5 37.0 84-85 29.689675524191117 34.0 30.0 35.0 6.5 36.5 86-87 29.358594700736838 34.0 29.5 35.0 2.0 36.0 88-89 28.95849683690102 34.0 29.0 35.0 2.0 36.0 90-91 28.436776149185157 34.0 29.0 35.0 2.0 35.0 92-93 27.972019839668533 33.0 27.5 35.0 2.0 35.0 94-95 27.426385265773394 33.0 27.0 35.0 2.0 35.0 96-97 26.874924139640257 33.0 25.5 35.0 2.0 35.0 98-99 26.016396958595337 32.5 24.5 34.0 2.0 35.0 100-101 23.496241139033373 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2291975209335959 1101 2 0.3579567805915289 1101 3 0.21419313125257844 1101 4 0.19528028405079567 1101 5 0.20900674830065924 1101 6 0.15972734902538122 1101 7 0.22627775757025148 1101 8 0.24094846172949502 1101 9 0.24860445942993437 1101 10-11 0.28076452001645436 1101 12-13 0.20030675035002332 1101 14-15 0.36200555618176367 1101 16-17 0.406701128768006 1101 18-19 0.35989421948839606 1101 20-21 0.389158058194937 1101 22-23 0.3770919803601558 1101 24-25 0.38626964191061575 1101 26-27 0.2825591138375856 1101 28-29 0.14417368764622296 1101 30-31 0.3639121750834491 1101 32-33 0.28934308019662325 1101 34-35 0.34378478868936213 1101 36-37 0.390916950054951 1101 38-39 0.414042696803115 1101 40-41 0.2852426664697134 1101 42-43 0.5068672434532999 1101 44-45 0.33459869813527376 1101 46-47 0.42561259095737825 1101 48-49 0.5157546692068635 1101 50-51 0.6073431706804229 1101 52-53 0.24543879827926673 1101 54-55 0.13226860937592022 1101 56-57 0.2999785420313188 1101 58-59 0.5587798267097099 1101 60-61 0.5752742215122879 1101 62-63 0.5566875260523574 1101 64-65 0.6483170048166045 1101 66-67 0.5163985128749005 1101 68-69 0.5110550435598142 1101 70-71 0.3825765206035001 1101 72-73 0.7562600299894946 1101 74-75 0.6789002643179458 1101 76-77 0.5316564390669534 1101 78-79 0.4284548111191562 1101 80-81 0.5705641774860304 1101 82-83 0.6790305870645312 1101 84-85 0.7769396785005682 1101 86-87 0.8061015286697 1101 88-89 0.8881328508759516 1101 90-91 0.878874897769709 1101 92-93 0.9752677163767842 1101 94-95 0.9857891036913422 1101 96-97 0.9797275572669157 1101 98-99 0.8061993728700543 1101 100-101 1.1468870373092486 1102 1 0.19035215901406133 1102 2 0.13019879971848525 1102 3 0.1633624478857172 1102 4 0.16538925224529777 1102 5 0.10430111581905521 1102 6 0.17735134680707887 1102 7 0.08598194461560382 1102 8 0.17154244753471914 1102 9 0.11738097138575654 1102 10-11 0.3164505712615906 1102 12-13 0.24981367959445322 1102 14-15 0.22723706197235316 1102 16-17 0.3548416782697714 1102 18-19 0.40901324083955615 1102 20-21 0.26726648361338334 1102 22-23 0.2955752752468541 1102 24-25 0.4236231071228005 1102 26-27 0.42154960935673813 1102 28-29 0.42852566022423133 1102 30-31 0.4686089579553183 1102 32-33 0.3386132823806989 1102 34-35 0.44191147380254847 1102 36-37 0.4078197046434653 1102 38-39 0.4027240459556154 1102 40-41 0.32904528477424577 1102 42-43 0.2972052395476723 1102 44-45 0.393013497042638 1102 46-47 0.3552670431960365 1102 48-49 0.14984764320671218 1102 50-51 0.28102192394740655 1102 52-53 0.4399886186321922 1102 54-55 0.2526336262786728 1102 56-57 0.39674533711565374 1102 58-59 0.6342610979856502 1102 60-61 0.6231048871799345 1102 62-63 0.35049678156418196 1102 64-65 0.3920249322970051 1102 66-67 0.5174501414980739 1102 68-69 0.6055150403739518 1102 70-71 0.4707193710038027 1102 72-73 0.5179710135351208 1102 74-75 0.5804041079984401 1102 76-77 0.3718442867828635 1102 78-79 0.30566278874734465 1102 80-81 0.5063580464764108 1102 82-83 0.5976916116133459 1102 84-85 0.3409430059538323 1102 86-87 0.1257804058312182 1102 88-89 0.305268055213439 1102 90-91 0.30534791530963545 1102 92-93 0.2056481316004728 1102 94-95 0.4534098566161404 1102 96-97 0.38994384172876906 1102 98-99 0.6669175776972232 1102 100-101 0.9397707711365868 1103 1 0.08907962369495692 1103 2 0.14982437292351847 1103 3 0.14444768921611484 1103 4 0.03213489830478977 1103 5 0.025265309141268233 1103 6 0.1663055145818788 1103 7 0.16570922393670173 1103 8 0.10790439880927494 1103 9 0.05529833315167565 1103 10-11 -0.01937464250661236 1103 12-13 0.20123007023619266 1103 14-15 0.16592165212571786 1103 16-17 0.14287791208101197 1103 18-19 0.04947970280453262 1103 20-21 0.1482880578822119 1103 22-23 0.22306252168673524 1103 24-25 0.023281400362002103 1103 26-27 -0.045246809169569246 1103 28-29 -0.1270016884053149 1103 30-31 -0.003824010299801728 1103 32-33 0.1537628193841627 1103 34-35 0.171927672637203 1103 36-37 0.005990284013726921 1103 38-39 -0.016688766436082858 1103 40-41 -0.12486459812671313 1103 42-43 -0.12633787272394414 1103 44-45 0.1376618372665277 1103 46-47 0.21886399040072746 1103 48-49 0.3398962397106615 1103 50-51 0.3354266369217811 1103 52-53 0.3635236717820973 1103 54-55 0.29520916039695777 1103 56-57 0.17654998400524136 1103 58-59 0.3848079093941408 1103 60-61 0.5117520024173174 1103 62-63 0.4659074341112728 1103 64-65 0.44217039944399517 1103 66-67 0.8513032881821516 1103 68-69 0.7521322240332751 1103 70-71 0.7955100353172 1103 72-73 0.5989047433772505 1103 74-75 0.7061417191135817 1103 76-77 0.5843286722345375 1103 78-79 0.3994097162071881 1103 80-81 0.5809927667845898 1103 82-83 0.7960983232499146 1103 84-85 0.6750508208841595 1103 86-87 0.6449997993017185 1103 88-89 0.794306037854092 1103 90-91 0.9417372675980218 1103 92-93 0.9680015563242463 1103 94-95 0.6455836435168436 1103 96-97 0.7886511586053793 1103 98-99 0.7787491742898283 1103 100-101 1.12730733940138 1104 1 0.3042315725523608 1104 2 0.3090534173393351 1104 3 0.20007480624297358 1104 4 0.11180666968974151 1104 5 0.1268253492156859 1104 6 0.18888077339586573 1104 7 0.19645203200883543 1104 8 0.17452335782840578 1104 9 0.14549441158304433 1104 10-11 0.2880725212737403 1104 12-13 0.18072532332526237 1104 14-15 0.30469842804290437 1104 16-17 0.3456640386971088 1104 18-19 0.4328965245210554 1104 20-21 0.37645942375216634 1104 22-23 0.19346469715546988 1104 24-25 0.3164210406463397 1104 26-27 0.2598388260577522 1104 28-29 0.39176650013530434 1104 30-31 0.42389890368331606 1104 32-33 0.268389984118663 1104 34-35 0.41500767836211594 1104 36-37 0.46229962040035133 1104 38-39 0.4044314091280583 1104 40-41 0.43538061918162185 1104 42-43 0.6542880516117222 1104 44-45 0.4556810157408364 1104 46-47 0.5505418683645686 1104 48-49 0.3530778627248381 1104 50-51 0.333642370903938 1104 52-53 0.494972553037762 1104 54-55 0.1550727165250052 1104 56-57 -0.0581143926943426 1104 58-59 0.09834256900007432 1104 60-61 0.19532625937070236 1104 62-63 0.23369045973026914 1104 64-65 0.508417238812342 1104 66-67 0.5322106876382833 1104 68-69 0.6502763965441289 1104 70-71 0.6892078550777043 1104 72-73 0.46869149019275724 1104 74-75 0.769736013278937 1104 76-77 0.40749550587797145 1104 78-79 0.6016090387585926 1104 80-81 0.6401263959053374 1104 82-83 0.6081778996060017 1104 84-85 0.5030075023401963 1104 86-87 0.6866676586595695 1104 88-89 0.826193829467087 1104 90-91 0.8262799897963937 1104 92-93 0.927712447980177 1104 94-95 1.029310564942346 1104 96-97 1.3058879316600986 1104 98-99 1.2653767206855164 1104 100-101 1.557563529468645 1105 1 0.12087290325577982 1105 2 0.12515201402011655 1105 3 0.04572225526743523 1105 4 0.08157186184522658 1105 5 0.2776023885513439 1105 6 0.09946785582359041 1105 7 0.25974587474160415 1105 8 0.2345105432750927 1105 9 0.24050301743502445 1105 10-11 0.34393517941412455 1105 12-13 0.21998182796740195 1105 14-15 0.1867354394880536 1105 16-17 0.20378934492725165 1105 18-19 0.2563182615211872 1105 20-21 0.21116927462594504 1105 22-23 0.13316342951974747 1105 24-25 0.30863682767348877 1105 26-27 0.27009298136511717 1105 28-29 0.17914165246516944 1105 30-31 0.3161888141070719 1105 32-33 0.18103493304889895 1105 34-35 0.1662005625160461 1105 36-37 -0.0018146050101464084 1105 38-39 0.1630441709155619 1105 40-41 0.1815318560372745 1105 42-43 0.12844034378353797 1105 44-45 0.3814470621998751 1105 46-47 0.4159526250513821 1105 48-49 0.1996512260425476 1105 50-51 0.08669839060317486 1105 52-53 0.4124870322266716 1105 54-55 0.6052178224963356 1105 56-57 0.3722234341081716 1105 58-59 0.6184978512343307 1105 60-61 0.2841637783726796 1105 62-63 0.5096116627906895 1105 64-65 0.481192594667462 1105 66-67 0.455467845571377 1105 68-69 0.1912477899383589 1105 70-71 0.40587305602749524 1105 72-73 0.4759254311704417 1105 74-75 0.7558711691265145 1105 76-77 0.6631571514173764 1105 78-79 0.5837729394081173 1105 80-81 0.6586434254416282 1105 82-83 0.4710232329728399 1105 84-85 0.7875219940923976 1105 86-87 0.8271817483865682 1105 88-89 0.8742358411006883 1105 90-91 0.9295979540412738 1105 92-93 0.8751865990160219 1105 94-95 1.4018861645252443 1105 96-97 1.278163268670287 1105 98-99 1.2981669012199575 1105 100-101 1.156341121588948 1106 1 -0.2582011522802219 1106 2 -0.3948125938081404 1106 3 -0.3799364234032865 1106 4 -0.31520473798750004 1106 5 -0.4446239598871742 1106 6 -0.4816386662173997 1106 7 -0.29457109832353723 1106 8 -0.24830843028734506 1106 9 -0.4368695659028816 1106 10-11 -0.6191048660531919 1106 12-13 -0.6470488786166442 1106 14-15 -0.6060669682129785 1106 16-17 -0.5381077529792506 1106 18-19 -0.38305778999121287 1106 20-21 -0.6117261666944174 1106 22-23 -0.7198944734940724 1106 24-25 -0.5964711410548134 1106 26-27 -0.5720711724358267 1106 28-29 -0.5816301939663902 1106 30-31 -0.8559411423308418 1106 32-33 -0.8697702711503652 1106 34-35 -1.2633848388589541 1106 36-37 -1.0181964012920375 1106 38-39 -1.053451939497684 1106 40-41 -1.2524872544437713 1106 42-43 -1.1288455674841558 1106 44-45 -1.05831358855108 1106 46-47 -0.941120804017352 1106 48-49 -1.1011257225451558 1106 50-51 -1.0138124708539067 1106 52-53 -1.0245191000483374 1106 54-55 -1.072860343827493 1106 56-57 -1.3946709996978157 1106 58-59 -1.4702828949075766 1106 60-61 -1.5139135483558377 1106 62-63 -1.4666423212505322 1106 64-65 -1.9630841101146785 1106 66-67 -2.1489358318561074 1106 68-69 -2.089399860970829 1106 70-71 -1.7408300792151685 1106 72-73 -1.6498168841105176 1106 74-75 -1.7555279518817741 1106 76-77 -1.4017484102557454 1106 78-79 -1.4930500319197044 1106 80-81 -1.7401724222428676 1106 82-83 -1.9519390530514507 1106 84-85 -1.5914858625822248 1106 86-87 -1.707020458457425 1106 88-89 -1.9027950915282439 1106 90-91 -2.32712930342063 1106 92-93 -2.2175224844365253 1106 94-95 -2.2862671455792842 1106 96-97 -2.279846309174733 1106 98-99 -2.5008275219296223 1106 100-101 -1.4042050028713682 1107 1 0.19219820446059543 1107 2 0.15023520392258405 1107 3 -0.09114521461208369 1107 4 0.05827868915446288 1107 5 0.11803841838496254 1107 6 0.10229057527281782 1107 7 0.032712025057151095 1107 8 0.0334319431603447 1107 9 0.295970602059235 1107 10-11 0.2069185414336303 1107 12-13 0.12382577518484084 1107 14-15 0.20151455595391354 1107 16-17 0.07473598864497433 1107 18-19 0.26266364304729706 1107 20-21 0.31474930904934695 1107 22-23 0.10816056090242654 1107 24-25 0.03642799233212202 1107 26-27 0.12132648681549085 1107 28-29 0.21408141150131144 1107 30-31 0.12278663395790801 1107 32-33 0.30149391182113305 1107 34-35 0.4576979807604502 1107 36-37 0.3854487340604109 1107 38-39 0.36026734934355886 1107 40-41 0.29000001438495815 1107 42-43 0.21401693586614812 1107 44-45 0.48914643110406075 1107 46-47 0.05428882700203985 1107 48-49 0.1372071640804151 1107 50-51 0.13034727184241746 1107 52-53 0.0942426260304643 1107 54-55 0.194294127717221 1107 56-57 0.22086370949543266 1107 58-59 0.3644531008040346 1107 60-61 0.5026950463015325 1107 62-63 0.4426581343913796 1107 64-65 0.5881461230667782 1107 66-67 0.45466463271996815 1107 68-69 0.12582036595671298 1107 70-71 0.15846054885594896 1107 72-73 0.25016524757893066 1107 74-75 0.3845917657989233 1107 76-77 0.21405789725788438 1107 78-79 0.5584946835274494 1107 80-81 0.6476967817238979 1107 82-83 0.6157621887961326 1107 84-85 0.6035288787739788 1107 86-87 0.6880997059310339 1107 88-89 0.9267430126440068 1107 90-91 0.994566399250683 1107 92-93 0.9779175227108006 1107 94-95 1.129505212144295 1107 96-97 1.131358908371947 1107 98-99 1.4740246178005663 1107 100-101 1.6533577595694453 1108 1 -0.004478516131669608 1108 2 0.002932397963348876 1108 3 0.046019578368451164 1108 4 0.12264209286527716 1108 5 0.02485132396945744 1108 6 -0.0673502253852547 1108 7 -0.07243010089111834 1108 8 0.008640790614876437 1108 9 -0.15162650972213498 1108 10-11 -0.0749715965793456 1108 12-13 -0.14212797675739353 1108 14-15 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-0.3331491773774644 2316 66-67 -0.1347048630325105 2316 68-69 0.09364672399583895 2316 70-71 -0.012096695318312811 2316 72-73 -0.08951988997855054 2316 74-75 -0.057471986052064494 2316 76-77 0.1634585675096183 2316 78-79 0.12033865515865472 2316 80-81 0.20863201670168863 2316 82-83 0.1369795529922193 2316 84-85 0.1348124887809945 2316 86-87 0.09224903178249022 2316 88-89 0.12818171578483728 2316 90-91 0.05289544609037122 2316 92-93 0.36977941967908023 2316 94-95 0.1464501112168506 2316 96-97 0.07820326968439772 2316 98-99 0.16279868061717906 2316 100-101 0.6415815924741288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 4.0 5 27.0 6 37.0 7 77.0 8 182.0 9 360.0 10 577.0 11 966.0 12 1394.0 13 2155.0 14 2706.0 15 3912.0 16 5361.0 17 7436.0 18 6947.0 19 4270.0 20 3552.0 21 3491.0 22 3426.0 23 3755.0 24 4211.0 25 4962.0 26 6067.0 27 7368.0 28 9057.0 29 11482.0 30 14279.0 31 18055.0 32 23198.0 33 30441.0 34 42481.0 35 62489.0 36 95754.0 37 140879.0 38 101317.0 39 6770.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.249342046711504 19.779919392775863 16.352046330082768 21.61869223042986 2 15.432459654998205 20.56030223402802 40.166273198971794 23.840964912001983 3 19.54496493742116 24.660892276700462 30.55496420661979 25.239178579258585 4 12.954884136208666 17.809470550293433 37.766702783082266 31.46894253041563 5 13.349920243020469 37.682948529738866 34.126136775275974 14.84099445196469 6 31.014733591126166 37.074824528235936 17.0645297610915 14.845912119546394 7 27.942349303991126 33.7526968159302 20.45242959682006 17.85252428325861 8 26.48821344483879 34.309217947210726 19.459334081080822 19.74323452686966 9 26.188743752442623 17.4626576386219 20.85993079628753 35.48866781264795 10-11 24.844069229131648 27.22989740184226 26.664161818488 21.261871550538093 12-13 26.381836496053484 24.468157784358834 27.530520355377046 21.619485364210632 14-15 22.82991710170531 25.692354864436346 26.85917457573803 24.618553458120314 16-17 21.408746739196054 29.044270676118366 27.58147640941399 21.96550617527159 18-19 21.24828817722251 27.029165329511983 30.697708778830908 21.0248377144346 20-21 21.94469422317403 25.736838426171587 31.170505492131173 21.14796185852321 22-23 21.613259405185563 25.770605617691917 31.29202147941027 21.32411349771225 24-25 21.91085494228258 25.891021628543193 31.22499785525685 20.973125573917383 26-27 21.381222272089985 26.23035463922439 31.245745242814078 21.142677845871546 28-29 21.018382227362597 26.778506909718885 31.157005558119565 21.04610530479895 30-31 21.297299530062944 26.162212485264817 30.96675489239744 21.573733092274793 32-33 21.174493125701012 26.250544129281938 30.94181232385304 21.633150421164007 34-35 21.943661569062318 26.051241885721733 30.752201141956576 21.252895403259377 36-37 21.8655050056121 25.710965320954145 30.969816989134053 21.453712684299703 38-39 21.3475818418101 26.410446646733792 30.949199772498353 21.29277173895775 40-41 21.37208175456156 26.395713684277418 30.928913566713533 21.30329099444749 42-43 21.46089697955863 26.08030674768636 30.42481312925862 22.03398314349639 44-45 21.65412124312491 26.342688650019223 30.244214753926467 21.7589753529294 46-47 22.145664600299312 26.88276675044404 29.74679321180848 21.22477543744817 48-49 22.103961261172522 26.9991389253407 29.35366973497329 21.54323007851349 50-51 21.786857013945596 27.1493503811288 29.58768504367332 21.476107561252274 52-53 22.294605097180696 27.398696631641155 28.880475847014676 21.42622242416347 54-55 21.67906381166931 27.128697298894583 29.303466858157805 21.888772031278297 56-57 22.19809165520156 27.14236010714184 29.07358216590462 21.585966071751987 58-59 22.48858520031901 26.840983976385584 29.057218569980588 21.61321225331482 60-61 21.84810134626322 26.96839760678438 29.91670453065076 21.266796516301635 62-63 21.638472561585903 27.19550207642911 29.786669547506854 21.379355814478128 64-65 21.372365540491163 27.263816117665375 29.391242457653238 21.972575884190224 66-67 21.481270831810832 27.213613240849888 29.21712108743244 22.087994839906838 68-69 21.729663863143145 26.96188394238743 29.399344820683588 21.909107373785837 70-71 22.00085471986477 27.204716528502843 29.075170864538023 21.719257887094365 72-73 21.757227784432658 27.014867041811367 29.35795922128348 21.86994595247249 74-75 21.67255014727236 27.618016477982227 28.76259440841629 21.94683896632912 76-77 21.749760900855673 27.07968594605415 29.12847170368864 22.042081449401536 78-79 21.785903794765556 27.304089628022105 28.878728278517933 22.031278298694406 80-81 21.976627065705397 27.660355296562372 28.65122663421485 21.711791003517376 82-83 22.064323230269157 27.41410700838515 28.94759836427589 21.573971397069805 84-85 21.746124168017726 27.69993589595922 28.833632141305323 21.720307794717733 86-87 21.761676140606184 27.847821735303746 28.606663637547943 21.783838486542134 88-89 21.485242578394335 27.769181152950374 28.392189322038746 22.35338694661655 90-91 21.71489267719901 27.380383022198636 28.753914672454528 22.150809628147826 92-93 21.962276800963416 27.10369907868585 29.06429180826371 21.869732312087027 94-95 21.664368349310344 27.09064796662462 28.955780162237904 22.28920352182713 96-97 21.59128821217388 26.853455260657782 29.333175522602417 22.22208100456592 98-99 21.53520223881012 27.270207000968316 28.52957741108586 22.6650133491357 100-101 21.265885538320735 26.93987091361509 29.090833976858516 22.703409571205665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 121.5 2 121.0 3 187.5 4 147.0 5 105.0 6 244.5 7 622.0 8 1160.0 9 1456.0 10 1215.0 11 926.5 12 917.5 13 962.0 14 1052.0 15 1145.0 16 1191.5 17 1267.5 18 1334.0 19 1380.5 20 1497.0 21 1700.0 22 1952.0 23 2269.5 24 2620.5 25 3095.5 26 3863.0 27 4849.0 28 5691.0 29 6546.0 30 7685.5 31 9017.0 32 10759.0 33 12249.0 34 13202.0 35 14108.5 36 15773.0 37 17380.0 38 18180.5 39 18391.0 40 18854.5 41 20363.5 42 21285.0 43 21993.0 44 23053.0 45 24152.0 46 24186.5 47 25657.5 48 28834.5 49 27316.0 50 23561.5 51 21555.5 52 20003.5 53 19210.5 54 18578.0 55 16968.5 56 14913.0 57 13403.5 58 12325.5 59 10608.0 60 8350.0 61 6700.0 62 5559.0 63 4240.5 64 3013.0 65 2176.0 66 1631.0 67 1298.5 68 943.0 69 609.5 70 426.0 71 304.5 72 244.0 73 194.0 74 126.5 75 105.0 76 86.5 77 45.0 78 26.0 79 23.5 80 21.0 81 13.5 82 5.0 83 3.0 84 3.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03526910966151187 2 0.0 3 0.0 4 0.0 5 0.005401575353564881 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004766095900204307 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.532191800408614E-4 24-25 0.0 26-27 5.560445216905024E-4 28-29 0.001032654111710933 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.9717465835035885E-4 38-39 0.0 40-41 1.5886986334014356E-4 42-43 0.021288561687579238 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 7.943493167007178E-5 86-87 0.0 88-89 0.0 90-91 0.013186198657231916 92-93 0.0030185274034627277 94-95 0.0 96-97 0.0 98-99 7.14914385030646E-4 100-101 0.015886986334014357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 629446.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.601901560541016 #Duplication Level Percentage of deduplicated Percentage of total 1 83.21088046206245 52.09159347451979 2 10.047175574834725 12.57944592594551 3 2.8069610380195673 5.271632957591249 4 1.195449057157511 2.993495367872643 5 0.6750121632983404 2.112852249948527 6 0.41455414492874476 1.5571126663406119 7 0.2895610914980314 1.26889524620058 8 0.20623957645347604 1.0328791730422544 9 0.15041282187257232 0.8474515801478967 >10 0.9063882539320351 10.559061748826021 >50 0.060310610839217065 2.5907367096252245 >100 0.03296635488509005 3.7216408943245614 >500 0.0025555313864294533 1.0827522491820607 >1k 0.0015333188318576722 2.290449756433151 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4333 0.688383117852842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3629 0.576538734061381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2113 0.33569202123772335 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1959 0.31122606228334126 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1252 0.19890506890185974 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1031 0.163794829103688 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 992 0.15759890443342242 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 762 0.12105883586518938 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 691 0.10977907556803919 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 689 0.1094613358413589 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 676 0.10739602761793705 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 664 0.10548958925785533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5886986334014356E-4 0.0 14-15 0.0 0.0 0.0 1.5886986334014356E-4 7.943493167007178E-5 16-17 0.0 0.0 0.0 1.5886986334014356E-4 1.5886986334014356E-4 18-19 0.0 0.0 0.0 2.3830479501021532E-4 1.5886986334014356E-4 20-21 0.0 0.0 0.0 3.177397266802871E-4 1.5886986334014356E-4 22-23 0.0 0.0 0.0 3.177397266802871E-4 1.5886986334014356E-4 24-25 0.0 0.0 0.0 4.7660959002043064E-4 1.5886986334014356E-4 26-27 0.0 0.0 0.0 9.532191800408613E-4 1.5886986334014356E-4 28-29 0.0 0.0 0.0 0.0016681335650715074 1.5886986334014356E-4 30-31 0.0 0.0 0.0 0.003495136993483158 1.5886986334014356E-4 32-33 0.0 0.0 0.0 0.005719315080245167 1.5886986334014356E-4 34-35 0.0 0.0 0.0 0.008022928098677249 1.5886986334014356E-4 36-37 0.0 0.0 0.0 0.01032654111710933 1.5886986334014356E-4 38-39 0.0 0.0 0.0 0.012947893862221699 1.5886986334014356E-4 40-41 0.0 0.0 0.0 0.017872859625766152 1.5886986334014356E-4 42-43 0.0 0.0 0.0 0.02764335622118498 1.5886986334014356E-4 44-45 0.0 0.0 0.0 0.041465034331777464 1.5886986334014356E-4 46-47 0.0 0.0 0.0 0.05441292819399916 1.5886986334014356E-4 48-49 0.0 0.0 0.0 0.08094419537180314 1.5886986334014356E-4 50-51 0.0 0.0 0.0 0.10914359611467862 1.5886986334014356E-4 52-53 0.0 0.0 0.0 0.13702525713087382 1.5886986334014356E-4 54-55 0.0 0.0 0.0 0.18476565106458698 1.5886986334014356E-4 56-57 1.5886986334014356E-4 0.0 0.0 0.23909914432691604 1.5886986334014356E-4 58-59 1.5886986334014356E-4 0.0 0.0 0.29438585676928597 1.5886986334014356E-4 60-61 1.5886986334014356E-4 0.0 0.0 0.3566628431986223 1.5886986334014356E-4 62-63 1.5886986334014356E-4 0.0 0.0 0.43816308309211593 1.5886986334014356E-4 64-65 1.5886986334014356E-4 0.0 0.0 0.4931320558078056 1.5886986334014356E-4 66-67 1.5886986334014356E-4 0.0 0.0 0.5754266450179999 1.5886986334014356E-4 68-69 1.5886986334014356E-4 0.0 0.0 0.6890980322378727 1.5886986334014356E-4 70-71 1.5886986334014356E-4 0.0 0.0 0.7817985974968464 1.5886986334014356E-4 72-73 1.5886986334014356E-4 0.0 0.0 0.9214452073728325 1.5886986334014356E-4 74-75 1.5886986334014356E-4 0.0 0.0 1.094613358413589 1.5886986334014356E-4 76-77 1.5886986334014356E-4 0.0 0.0 1.2368018861030174 1.5886986334014356E-4 78-79 1.5886986334014356E-4 0.0 0.0 1.378990413792446 1.5886986334014356E-4 80-81 1.5886986334014356E-4 0.0 0.0 1.5843297121595816 1.5886986334014356E-4 82-83 1.5886986334014356E-4 0.0 0.0 1.7995983769854762 1.5886986334014356E-4 84-85 1.5886986334014356E-4 0.0 0.0 2.026385106903531 1.5886986334014356E-4 86-87 1.5886986334014356E-4 0.0 0.0 2.30234205952536 1.5886986334014356E-4 88-89 1.5886986334014356E-4 0.0 0.0 2.633029680067869 1.5886986334014356E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGTGC 70 2.0508998E-4 33.932457 8 GCCGTAT 35 0.008392057 27.142733 92-93 CTTACGG 70 0.008390551 27.141653 3 GTATCAA 5685 0.0 25.574276 1 GGTATCA 4175 0.0 24.240162 1 GAGTACT 3395 0.0 23.927631 12-13 CCGGGTG 100 0.0016517155 23.75272 9 GGCCTAA 140 1.7784325E-5 23.748947 6 GTACTTT 3535 0.0 22.909168 14-15 TAAGGTG 375 0.0 22.79899 5 TCAACGC 6555 0.0 22.028008 4 TATCAAC 6585 0.0 21.999784 2 CAACGCA 6615 0.0 21.900011 5 ATCAACG 6625 0.0 21.79526 3 AACGCAG 6695 0.0 21.638323 6 GCGCTTT 155 3.8993607E-5 21.454071 7 GTAAGGT 310 1.0913936E-11 21.450663 4 AGAGTAC 4855 0.0 21.135273 10-11 AGTACTT 3500 0.0 20.766666 12-13 ACTTTTT 4025 0.0 20.120226 16-17 >>END_MODULE