FastQCFastQC Report
Thu 26 May 2016
SRR937608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494459
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.519962221336855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19570.3957861015776839No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17040.3446190685173088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.2325774230017049No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9750.1971852064579672No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6750.13651283524013114No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6410.12963663316877638No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.11325509293996065No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5440.11001923314167607No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5400.10921026819210491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA250.005016215657.0096637
AGGACCG1056.871041E-527.1584645
GGACCGA950.001224352725.0042386
GTGTAGC2301.3278623E-1024.8119471
CACCGTG1351.3389494E-524.6338067
GAGTACT21650.023.80285812-13
GCACCGT1000.001650683723.7540266
GTACTTT22500.023.11122314-15
GTACATG21550.022.9505731
GTATCAA43100.022.6195551
TAAGGCA1903.260957E-722.5129384
GGTATCA33400.022.2118911
CTTATGC1503.0129158E-522.181663
GTAAGCG652.9457916E-421.91791394-95
TACATGG22400.021.8562932
ACATGGG21300.021.4229323
AATCGAG958.255287E-619.99832580-81
GTATTGA1200.004727248619.8150961
CGGTTCA600.004763943619.79101234-35
ACTTTTT26400.019.78700316-17
Produced by FastQC (version 0.11.5)