##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937608_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 494459 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85934324180569 33.0 31.0 34.0 30.0 34.0 2 31.9678861139144 33.0 31.0 34.0 30.0 34.0 3 32.01119203007732 34.0 31.0 34.0 30.0 34.0 4 35.60347571790583 37.0 35.0 37.0 33.0 37.0 5 35.60479028594889 37.0 35.0 37.0 33.0 37.0 6 35.67645649083139 37.0 35.0 37.0 35.0 37.0 7 35.61555963184005 37.0 35.0 37.0 33.0 37.0 8 35.61132470032905 37.0 35.0 37.0 33.0 37.0 9 37.1804821026617 39.0 37.0 39.0 34.0 39.0 10-11 37.1223195451999 39.0 37.0 39.0 34.0 39.0 12-13 37.029925635897015 39.0 37.0 39.0 33.0 39.0 14-15 38.16688744668416 40.0 38.0 41.0 33.0 41.0 16-17 38.08398370744592 40.0 38.0 41.0 33.0 41.0 18-19 38.030476743268906 40.0 38.0 41.0 33.0 41.0 20-21 38.05146938371028 40.0 38.0 41.0 33.5 41.0 22-23 38.06254209145753 40.0 38.0 41.0 33.0 41.0 24-25 37.981504027634244 40.0 38.0 41.0 33.0 41.0 26-27 37.8738479833515 40.0 38.0 41.0 33.0 41.0 28-29 37.736205630800534 40.0 38.0 41.0 33.0 41.0 30-31 37.49096386151329 40.0 37.0 41.0 32.5 41.0 32-33 37.34106568997632 40.0 37.0 41.0 32.0 41.0 34-35 37.30543280636009 40.0 37.0 41.0 32.0 41.0 36-37 37.146518720460136 40.0 36.5 41.0 31.5 41.0 38-39 36.9748442236869 40.0 36.0 41.0 31.0 41.0 40-41 36.77261815438692 39.5 36.0 41.0 30.5 41.0 42-43 36.50107390097055 39.0 36.0 41.0 30.0 41.0 44-45 36.213789212047914 39.0 35.0 41.0 30.0 41.0 46-47 35.8232027326836 39.0 35.0 40.5 28.5 41.0 48-49 35.43545066426134 39.0 35.0 40.0 27.0 41.0 50-51 34.55576397638632 38.0 34.0 39.5 25.0 40.5 52-53 34.657355817165836 38.0 34.5 39.5 26.0 40.5 54-55 34.92462266841133 38.0 35.0 40.0 26.0 41.0 56-57 34.66971477918291 38.0 34.0 40.0 24.5 41.0 58-59 34.39192430514967 38.0 34.0 40.0 23.5 41.0 60-61 34.02574328710773 38.0 34.0 40.0 23.0 41.0 62-63 33.67666075448116 37.5 33.0 40.0 22.0 41.0 64-65 33.24370776950162 37.0 33.0 39.5 20.5 41.0 66-67 32.71812324176524 36.0 32.0 39.0 18.0 40.5 68-69 32.25407768894893 35.5 31.5 39.0 17.0 40.0 70-71 31.759351129213947 35.0 31.0 38.0 12.5 40.0 72-73 31.286207754333525 35.0 31.0 37.5 8.0 39.5 74-75 30.726165364570164 35.0 30.0 37.0 6.5 39.0 76-77 30.176891309491786 34.0 29.5 36.0 2.0 39.0 78-79 29.6455368392526 34.0 29.0 36.0 2.0 38.5 80-81 29.080760993328063 34.0 29.0 35.5 2.0 37.0 82-83 28.53276409166382 34.0 28.0 35.0 2.0 37.0 84-85 27.980929662520047 33.5 26.5 35.0 2.0 36.0 86-87 27.40163289575071 33.0 26.0 35.0 2.0 36.0 88-89 26.842742269834304 33.0 25.0 35.0 2.0 35.5 90-91 26.258114424047292 32.0 24.5 35.0 2.0 35.0 92-93 25.585788710489645 32.0 21.5 34.0 2.0 35.0 94-95 24.791088846598 31.5 18.5 34.0 2.0 35.0 96-97 23.884788223088265 31.0 4.5 34.0 2.0 35.0 98-99 22.666900592364584 31.0 2.0 34.0 2.0 35.0 100-101 19.425532753979603 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07494482423400584 1101 2 0.18929094231558707 1101 3 0.10836582624314772 1101 4 -0.008844277036828885 1101 5 -0.10258141071310689 1101 6 -0.058830699201919856 1101 7 0.17019657019351087 1101 8 -0.20580013222448912 1101 9 0.21128728797910412 1101 10-11 -0.0497140082097971 1101 12-13 0.06972185141413689 1101 14-15 0.22446492587673106 1101 16-17 0.04116016136578793 1101 18-19 0.2978938545346068 1101 20-21 0.11313200376967814 1101 22-23 0.2066288771480842 1101 24-25 0.02617235537477569 1101 26-27 0.05164558359351901 1101 28-29 -0.09146368089323431 1101 30-31 0.030060812945357895 1101 32-33 -0.004401012357725165 1101 34-35 0.0026180355607934302 1101 36-37 0.3103530874656144 1101 38-39 -0.11393440219097073 1101 40-41 0.0017141581079584967 1101 42-43 -0.19716358767665554 1101 44-45 -0.18465133626170172 1101 46-47 -0.18370793859318013 1101 48-49 -0.03463933137856401 1101 50-51 -0.031193092189788274 1101 52-53 -0.04736269667163384 1101 54-55 0.10879410071449769 1101 56-57 0.23246782397229282 1101 58-59 0.2177979276235078 1101 60-61 0.4761256334916766 1101 62-63 0.4004890385037001 1101 64-65 0.4727718852339322 1101 66-67 0.7670442338383836 1101 68-69 0.61750260803489 1101 70-71 0.5016767585526338 1101 72-73 0.9026234593645164 1101 74-75 1.0293063523952668 1101 76-77 0.8266254328513725 1101 78-79 1.0874068604249665 1101 80-81 1.025626780619774 1101 82-83 1.173641800624118 1101 84-85 0.9957811291622001 1101 86-87 1.2054897269167135 1101 88-89 1.0450757152224774 1101 90-91 1.1521499353523375 1101 92-93 1.2752114865983586 1101 94-95 1.452970423795886 1101 96-97 1.7681340256856366 1101 98-99 2.1733989174955077 1101 100-101 2.982831395109475 1102 1 0.2864878130701669 1102 2 0.24130832205546682 1102 3 0.050196563766949254 1102 4 0.18123423742494538 1102 5 0.23724247899493633 1102 6 0.15605152910541165 1102 7 0.18202743162230206 1102 8 0.2196668863323481 1102 9 0.3953274992784088 1102 10-11 0.3905328105295922 1102 12-13 0.1796379677361415 1102 14-15 0.5082290355574202 1102 16-17 0.6505094123601154 1102 18-19 0.6215687939244177 1102 20-21 0.4694786238480759 1102 22-23 0.5761662159167145 1102 24-25 0.2742425351609157 1102 26-27 0.4562263510610194 1102 28-29 0.4894658956408122 1102 30-31 0.5748978985347932 1102 32-33 0.6293430660163111 1102 34-35 0.729202057599359 1102 36-37 0.8709828965280266 1102 38-39 0.9495276684266116 1102 40-41 0.6977545676552523 1102 42-43 0.9742527702600796 1102 44-45 1.0916929296218925 1102 46-47 1.0856852573708196 1102 48-49 1.1462702138488297 1102 50-51 0.9953432264277566 1102 52-53 1.0190173364523005 1102 54-55 1.2171614046658235 1102 56-57 1.161863663242734 1102 58-59 1.097966314504447 1102 60-61 1.1678737676890378 1102 62-63 1.1874127254205646 1102 64-65 1.2112022568249898 1102 66-67 1.4312204102130082 1102 68-69 1.3414402260027671 1102 70-71 1.6409232510088856 1102 72-73 1.7757323594599193 1102 74-75 1.7060103557654855 1102 76-77 1.8384397736148728 1102 78-79 1.9938802766094348 1102 80-81 2.354290960926317 1102 82-83 2.510000480788168 1102 84-85 2.396293682802739 1102 86-87 2.139962573138231 1102 88-89 2.18958507211428 1102 90-91 2.499058092843839 1102 92-93 2.3870873668400563 1102 94-95 2.463128567864345 1102 96-97 2.291756511963161 1102 98-99 2.453832048037672 1102 100-101 2.839633366024845 1103 1 0.18721437286719578 1103 2 0.19729263759000304 1103 3 0.22790386396300377 1103 4 0.12777788867624906 1103 5 0.23466376835025926 1103 6 0.19931297530538927 1103 7 0.12466149920389569 1103 8 0.0971665980149723 1103 9 0.17792004915731496 1103 10-11 0.3377303655982189 1103 12-13 0.27190875541921855 1103 14-15 0.37849544081633013 1103 16-17 0.372819411206855 1103 18-19 0.3338972450834561 1103 20-21 0.3082538516188009 1103 22-23 -0.1511524324514113 1103 24-25 0.006763582329632811 1103 26-27 0.06958582517012246 1103 28-29 0.13710169312668796 1103 30-31 0.24548145291145573 1103 32-33 0.39311541061324107 1103 34-35 0.351509749907045 1103 36-37 0.318356901833063 1103 38-39 0.6569005204621305 1103 40-41 0.6216145607356367 1103 42-43 0.6428999851141839 1103 44-45 0.6022441924520123 1103 46-47 0.6507400376729819 1103 48-49 0.6374119506727212 1103 50-51 0.3439223983802506 1103 52-53 0.6040698102154138 1103 54-55 0.49022448850852385 1103 56-57 0.4935612759748338 1103 58-59 0.5242124222198186 1103 60-61 0.7826079390648886 1103 62-63 0.90793976959079 1103 64-65 0.8273745552911436 1103 66-67 1.069349807053051 1103 68-69 1.0577463037331327 1103 70-71 1.0934546776032832 1103 72-73 1.1009451376861925 1103 74-75 1.1590711330691406 1103 76-77 0.988786907740117 1103 78-79 1.2397654109653367 1103 80-81 1.2603502389795942 1103 82-83 1.3152374776743407 1103 84-85 1.363611177901344 1103 86-87 1.342876885212963 1103 88-89 1.749638122512156 1103 90-91 1.9459431118727828 1103 92-93 1.796703328090203 1103 94-95 2.2744029306829354 1103 96-97 2.346112410936751 1103 98-99 2.810014725929495 1103 100-101 3.8796633510323417 1104 1 -0.017177646206718578 1104 2 0.06800916413035196 1104 3 -0.2424311843492042 1104 4 -0.029044799239429153 1104 5 -0.09962660685366131 1104 6 0.03672293891437306 1104 7 -0.011192434790331163 1104 8 0.10165900609628409 1104 9 0.16982116598502728 1104 10-11 0.1589940122289022 1104 12-13 -0.03630055566121371 1104 14-15 0.16496551234349965 1104 16-17 0.15330002357979566 1104 18-19 0.228250363416997 1104 20-21 0.23725623585772837 1104 22-23 0.07881619598377654 1104 24-25 0.04379332257308022 1104 26-27 0.005254039500655949 1104 28-29 0.009187274755497299 1104 30-31 0.15286380954339762 1104 32-33 0.04249048904215158 1104 34-35 0.19494430882284774 1104 36-37 0.3765800164020163 1104 38-39 0.3876609671932201 1104 40-41 0.13701046988358456 1104 42-43 -0.05803614237984789 1104 44-45 -0.06353695951185045 1104 46-47 0.08874857073860909 1104 48-49 0.2437728491543396 1104 50-51 0.022656242024368112 1104 52-53 0.11961630287446923 1104 54-55 5.808694847999618E-4 1104 56-57 0.032229866764140525 1104 58-59 0.4616299150043588 1104 60-61 0.4894042748661178 1104 62-63 0.4213153499647362 1104 64-65 0.6348322036786485 1104 66-67 0.6794539606482246 1104 68-69 0.5647722793887979 1104 70-71 0.5200954104430338 1104 72-73 0.5278644500214931 1104 74-75 0.39854785536804727 1104 76-77 0.8081820639705093 1104 78-79 0.5431623240033581 1104 80-81 0.6762543674775259 1104 82-83 0.7514666155837411 1104 84-85 1.3906170757555252 1104 86-87 1.1887383483827456 1104 88-89 1.5131492280460996 1104 90-91 1.3951010990731874 1104 92-93 1.642678983755907 1104 94-95 1.8010260954463924 1104 96-97 1.7926577742194674 1104 98-99 2.3263982119377786 1104 100-101 3.2894512792542017 1105 1 0.22439386004668194 1105 2 0.32524135553872213 1105 3 0.16812822293736573 1105 4 0.27870737585573124 1105 5 0.167580435016923 1105 6 0.16088348812590425 1105 7 0.20043072997312805 1105 8 0.2532563416047182 1105 9 0.4047790235162907 1105 10-11 0.3373297245725695 1105 12-13 0.36137990926537356 1105 14-15 0.5111556972265845 1105 16-17 0.3765854368478756 1105 18-19 0.47187801431422116 1105 20-21 0.49185962084956714 1105 22-23 0.34423218293320446 1105 24-25 0.18992063361168476 1105 26-27 0.18647685081114673 1105 28-29 0.4250824623574587 1105 30-31 0.05859042727043118 1105 32-33 0.3303269490747809 1105 34-35 0.4823270575993561 1105 36-37 0.4224915172176793 1105 38-39 0.4874774435390563 1105 40-41 0.4823357145817866 1105 42-43 0.26972690819111733 1105 44-45 0.454071115528933 1105 46-47 0.09431375562169819 1105 48-49 -0.11380599804522973 1105 50-51 0.04605380863665687 1105 52-53 0.23698647688208752 1105 54-55 0.33570525773928495 1105 56-57 0.43194268623124543 1105 58-59 0.48267396068135326 1105 60-61 0.5029765288084747 1105 62-63 0.6858884875395077 1105 64-65 0.602102119393713 1105 66-67 0.6543978839761238 1105 68-69 0.7405828421946765 1105 70-71 0.7796726263212292 1105 72-73 0.8606727017887543 1105 74-75 1.1495198510178568 1105 76-77 1.0092356256888344 1105 78-79 1.1617525904525152 1105 80-81 1.108090623594979 1105 82-83 1.2911349135717778 1105 84-85 1.6261272035423673 1105 86-87 1.4583736800847547 1105 88-89 1.4810003019993339 1105 90-91 1.4286995221291967 1105 92-93 1.0792834562953288 1105 94-95 1.4591945973496045 1105 96-97 1.2993495904239296 1105 98-99 1.0792615208012926 1105 100-101 2.5202883510323417 1106 1 -0.009060538661955775 1106 2 -0.013782050433835735 1106 3 -0.052427668910898007 1106 4 -0.13832753132747655 1106 5 -0.2808810258386032 1106 6 -0.20479952236642873 1106 7 0.12845736435568966 1106 8 0.15132938438114962 1106 9 0.15248887016681323 1106 10-11 -0.22165685734833573 1106 12-13 -0.13051627717530323 1106 14-15 -0.2195898637096576 1106 16-17 -0.3439527996331577 1106 18-19 -0.3782279178891699 1106 20-21 -0.15389552442887577 1106 22-23 -0.20709953619512333 1106 24-25 -0.5058849555731442 1106 26-27 -0.10207742683211052 1106 28-29 -0.0027474407902374764 1106 30-31 0.1636095959548527 1106 32-33 -0.16651021799544452 1106 34-35 -0.08072530317546267 1106 36-37 -0.3569122358089558 1106 38-39 -0.7732671085190574 1106 40-41 -0.06441822004290998 1106 42-43 -0.10840565839484384 1106 44-45 -0.0012406278124714731 1106 46-47 0.12186873947964472 1106 48-49 -0.03988549764167715 1106 50-51 -0.1604862438250052 1106 52-53 -0.3086904803414825 1106 54-55 -0.16715491175224173 1106 56-57 -0.0819369546081461 1106 58-59 -0.04856090614673292 1106 60-61 0.034788978364574064 1106 62-63 0.045412297063315066 1106 64-65 -0.20212255776529275 1106 66-67 0.08414213337079701 1106 68-69 0.015650941468280166 1106 70-71 -0.16089436318643635 1106 72-73 -0.18828563154457711 1106 74-75 0.1212409971034063 1106 76-77 0.48725217129012677 1106 78-79 0.3747570295162781 1106 80-81 0.0558130812221016 1106 82-83 0.30086049872108944 1106 84-85 0.4776379985383308 1106 86-87 0.2537858067998471 1106 88-89 0.704363397237433 1106 90-91 0.9264497239048204 1106 92-93 0.7707357363598959 1106 94-95 0.967671695816108 1106 96-97 1.1620967655651704 1106 98-99 1.5466621463057315 1106 100-101 1.733658508417328 1107 1 0.07795373059684252 1107 2 0.28705365327335386 1107 3 0.2022503912221545 1107 4 0.3045366638816205 1107 5 0.15922687514637346 1107 6 0.1361464331097224 1107 7 0.07688704065273555 1107 8 0.24302171377299686 1107 9 0.2916317743253529 1107 10-11 0.08270796081852438 1107 12-13 0.3474539383915882 1107 14-15 0.37132964544665015 1107 16-17 0.49765137535920445 1107 18-19 0.32108917936276526 1107 20-21 0.36767879560684946 1107 22-23 0.2816108237099044 1107 24-25 0.5098741125760924 1107 26-27 0.4604857504875213 1107 28-29 0.16531304488172793 1107 30-31 0.23312521691443777 1107 32-33 0.38366432771555736 1107 34-35 0.47434768866732213 1107 36-37 0.4933046240137955 1107 38-39 0.6082298707235267 1107 40-41 0.626480132057516 1107 42-43 0.6218506945988764 1107 44-45 0.6460007271794197 1107 46-47 0.7647728980165169 1107 48-49 1.0046001508220854 1107 50-51 0.7359911063712872 1107 52-53 0.9055746645843499 1107 54-55 1.0590669082247288 1107 56-57 1.1583484305678056 1107 58-59 0.9945671645648559 1107 60-61 1.4692285514622014 1107 62-63 1.4341085522644264 1107 64-65 1.6072699996526083 1107 66-67 1.5240884179567047 1107 68-69 1.8171301722917619 1107 70-71 1.8306839531820742 1107 72-73 1.8821027179699819 1107 74-75 1.72990010991753 1107 76-77 1.7374314185690949 1107 78-79 1.5299969270220934 1107 80-81 1.7707423226241055 1107 82-83 1.9373323245750136 1107 84-85 2.1119382876847617 1107 86-87 2.3145124341300622 1107 88-89 2.005160900704837 1107 90-91 2.111021528601686 1107 92-93 2.419261207104391 1107 94-95 1.8731407947606051 1107 96-97 1.8887711114595298 1107 98-99 2.545533769992229 1107 100-101 3.217770147148844 1108 1 0.09596768851706017 1108 2 0.0021202237969042415 1108 3 0.1422274713900613 1108 4 0.18676993111656515 1108 5 0.39996880813805546 1108 6 0.208755946127674 1108 7 0.014688024402836675 1108 8 0.11414272533592396 1108 9 0.377336494780657 1108 10-11 0.34953407912606593 1108 12-13 0.17387162014070157 1108 14-15 0.32157236389325305 1108 16-17 0.2082305517904004 1108 18-19 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-0.711666179410912 2316 76-77 -0.616680695196365 2316 78-79 -0.7334583362418137 2316 80-81 -0.8228352062805406 2316 82-83 -0.9296811306882518 2316 84-85 -1.3447450302059032 2316 86-87 -1.4315597569830167 2316 88-89 -1.2905980728277733 2316 90-91 -1.4075902427394062 2316 92-93 -1.4798494966783906 2316 94-95 -1.6955555755411318 2316 96-97 -2.168605112204009 2316 98-99 -2.4822227461420034 2316 100-101 -2.6518719186772017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2018.0 3 364.0 4 342.0 5 391.0 6 453.0 7 522.0 8 609.0 9 722.0 10 1053.0 11 1324.0 12 1712.0 13 2273.0 14 2612.0 15 3301.0 16 4300.0 17 5814.0 18 4946.0 19 3510.0 20 3388.0 21 3600.0 22 3903.0 23 4309.0 24 4999.0 25 5563.0 26 6456.0 27 7427.0 28 8923.0 29 10653.0 30 13035.0 31 16185.0 32 20890.0 33 27184.0 34 36857.0 35 52280.0 36 74103.0 37 93997.0 38 60059.0 39 4382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.091452703662085 19.4578882672952 15.69499267745508 21.75566635158763 2 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20.986863834107023 20-21 22.17656433152897 26.63732674400816 30.024487083240885 21.161621841221983 22-23 21.914486822654233 26.560475156516127 30.16254981209959 21.36248820873005 24-25 21.847083485733798 26.818192410608898 30.136993241018335 21.197730862638974 26-27 21.352534926418436 26.990917307372747 30.218090000121588 21.438457766087225 28-29 21.184897561726928 27.675002305228464 30.103142507703517 21.0369576253411 30-31 21.469978771589396 26.973456887073976 29.895073894121403 21.66149044721523 32-33 21.161523838861214 27.185786258861295 29.92990000770107 21.722789894576422 34-35 22.024383719327826 27.093854621801057 29.61886252543348 21.262899133437635 36-37 21.868224499803446 26.699270120322755 29.919494393178603 21.513010986695196 38-39 21.298524201694125 27.35267091430198 29.873999426579363 21.47480545742453 40-41 21.588385700739924 27.302639576630387 29.719752656190483 21.389222066439206 42-43 21.398705405221943 27.261665587668826 29.28764866614591 22.05198034096332 44-45 21.680389778940874 27.280675892407558 29.243450747248538 21.79548358140303 46-47 22.203196289200037 27.720660575891348 28.709457453566873 21.36668568134174 48-49 21.891409335854455 27.761125669039842 28.61170052145082 21.73576447365488 50-51 21.65444007619665 28.039050784258094 28.71326956592226 21.593239573622988 52-53 22.218067475119653 28.05842587779166 28.074436854430836 21.64906979265785 54-55 21.603504125331447 27.86328804570845 28.522084683028844 22.011123145931258 56-57 22.00888402647987 27.98002360294378 28.29172428114854 21.719368089427807 58-59 22.35118839628537 27.543832462811658 28.280661454677674 21.824317686225296 60-61 21.70459944425366 27.87251264003825 28.97529329649969 21.447594619208402 62-63 21.630269959269587 28.069747159456377 28.86804703699164 21.431935844282396 64-65 21.375590839929735 28.15386454393134 28.532528865810196 21.938015750328724 66-67 21.57840407071273 28.012083282260324 28.2354538170023 22.174058830024645 68-69 21.607992860478472 27.798245711779302 28.648748304515586 21.94501312322664 70-71 21.761809198963057 27.93888969368869 28.269544225550153 22.029756881798097 72-73 21.67334818565606 27.775261865901555 28.580387436920685 21.971002511521696 74-75 21.393110435663626 28.605471124620063 28.13343465045593 21.867983789260386 76-77 21.713969618314113 27.9403510691541 28.101641064919225 22.244038247612565 78-79 21.762999719374033 28.138477264737677 28.064420375674086 22.034102640214208 80-81 21.989643898846158 28.170682163332998 27.994196855076385 21.84547708274446 82-83 21.841459325466168 28.22119636404526 28.269922939073545 21.667421371415028 84-85 21.676340393634586 28.51358880076174 27.987864790672706 21.822206014930966 86-87 21.83804766569385 28.474513430858206 27.809782944527118 21.87765595892083 88-89 21.57112436202135 28.42087241237481 27.61578843928996 22.392214786313875 90-91 21.728295258037754 28.106426453440957 27.938463595722112 22.226814692799174 92-93 22.13109605998659 28.120435109415553 28.02946652700592 21.719002303591942 94-95 21.70239751040623 27.937727739176154 28.006714285280136 22.35316046513748 96-97 21.589777411710834 28.06176670526579 28.101480240996324 22.246975642027053 98-99 21.677393602741017 28.359736150804544 27.32770122756132 22.635169018893116 100-101 21.95977736248487 27.21353652298545 28.28590384449813 22.54078227003155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 691.0 1 402.0 2 112.0 3 116.5 4 119.0 5 130.5 6 221.5 7 397.5 8 647.5 9 803.0 10 740.0 11 646.5 12 637.0 13 704.0 14 768.5 15 801.0 16 804.5 17 864.5 18 925.0 19 968.5 20 1097.0 21 1242.0 22 1419.5 23 1727.0 24 2040.5 25 2422.5 26 3093.5 27 3931.5 28 4655.5 29 5340.5 30 6272.0 31 7413.5 32 8759.5 33 9886.0 34 10672.5 35 11517.0 36 12709.0 37 13742.5 38 14386.5 39 14770.5 40 15171.5 41 16031.5 42 16844.5 43 17501.0 44 18055.5 45 18900.0 46 18896.5 47 19730.0 48 22287.5 49 21545.5 50 18690.0 51 16705.0 52 15387.0 53 14894.0 54 14491.0 55 13234.5 56 11733.0 57 10575.0 58 9376.0 59 8053.0 60 6484.5 61 5228.0 62 4257.5 63 3191.0 64 2309.0 65 1728.5 66 1277.0 67 985.0 68 738.5 69 478.0 70 338.0 71 272.0 72 218.5 73 167.0 74 112.0 75 94.0 76 72.0 77 42.0 78 29.0 79 20.0 80 12.5 81 9.0 82 9.0 83 7.0 84 4.0 85 3.0 86 2.5 87 2.0 88 1.0 89 1.0 90 2.0 91 2.0 92 0.5 93 0.5 94 1.0 95 1.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29547444783086163 2 0.256239647776661 3 0.25927326633755277 4 0.2542172354027331 5 0.25482395911491146 6 0.20365692605453636 7 0.19576951779621768 8 0.20203899615539406 9 0.20345468481714357 10-11 0.20557821780976784 12-13 0.20992640441371274 14-15 0.20507261471628588 16-17 0.19607287965230685 18-19 0.1892977981996485 20-21 0.18879219510616652 22-23 0.19809529202623474 24-25 0.19566839717752132 26-27 0.20082554873103736 28-29 0.20537597657237505 30-31 0.20557821780976784 32-33 0.2064883033780354 34-35 0.20527485595367867 36-37 0.19273589923532589 38-39 0.1879832301565954 40-41 0.18100590746654424 42-43 0.19121908995488 44-45 0.19182581366705834 46-47 0.19829753326362753 48-49 0.2079039920397849 50-51 0.20203899615539406 52-53 0.21184769616894422 54-55 0.19890425697580588 56-57 0.1772644445747777 58-59 0.15966945692160525 60-61 0.17969133942349114 62-63 0.17139944869038687 64-65 0.178376771380438 66-67 0.17888237447391997 68-69 0.17564651467563538 70-71 0.18050030437306228 72-73 0.18879219510616652 74-75 0.1939493466596826 76-77 0.18970228067443407 78-79 0.18596081778266751 80-81 0.18879219510616652 82-83 0.17969133942349114 84-85 0.17271401673343997 86-87 0.1770622033373849 88-89 0.18363504355265048 90-91 0.1815115105600262 92-93 0.178376771380438 94-95 0.17888237447391997 96-97 0.18697202396963145 98-99 0.18596081778266751 100-101 0.16816358889210228 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 494459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7308145486457 #Duplication Level Percentage of deduplicated Percentage of total 1 85.82876227422327 58.132519805352146 2 8.659681818079655 11.730546065412643 3 2.290371030369858 4.653860865167143 4 0.9610277584980904 2.603647715477393 5 0.5268670344870869 1.7842566702319897 6 0.34778772719673684 1.4133567631834298 7 0.25566099808295467 1.2121289353934785 8 0.171399107205663 0.9287200915160163 9 0.13646827170662007 0.8318796482461748 >10 0.7517809001724353 9.32504934565309 >50 0.04211584876579795 1.9411438403742882 >100 0.02509029172115933 3.1533723754451515 >500 0.001792163694368517 0.796081368921322 >1k 0.0011947757962456778 1.4934365096257178 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2571 0.519962221336855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1957 0.3957861015776839 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1704 0.3446190685173088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1150 0.2325774230017049 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 975 0.1971852064579672 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 675 0.13651283524013114 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 641 0.12963663316877638 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 560 0.11325509293996065 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 544 0.11001923314167607 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 540 0.10921026819210491 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0224123739278686E-4 0.0 10-11 0.0 0.0 0.0 2.0224123739278686E-4 0.0 12-13 0.0 0.0 0.0 2.0224123739278686E-4 0.0 14-15 2.0224123739278686E-4 0.0 0.0 2.0224123739278686E-4 0.0 16-17 2.0224123739278686E-4 0.0 0.0 2.0224123739278686E-4 0.0 18-19 2.0224123739278686E-4 0.0 0.0 4.044824747855737E-4 0.0 20-21 2.0224123739278686E-4 0.0 0.0 5.056030934819672E-4 0.0 22-23 2.0224123739278686E-4 0.0 0.0 8.089649495711474E-4 0.0 24-25 2.0224123739278686E-4 0.0 0.0 8.089649495711474E-4 0.0 26-27 2.0224123739278686E-4 0.0 0.0 0.0010112061869639343 0.0 28-29 2.0224123739278686E-4 0.0 0.0 0.0018201711365350816 0.0 30-31 2.0224123739278686E-4 0.0 0.0 0.004348186603944917 0.0 32-33 2.0224123739278686E-4 0.0 0.0 0.00647171959656918 0.0 34-35 2.0224123739278686E-4 0.0 0.0 0.008797493826586228 0.0 36-37 2.0224123739278686E-4 0.0 0.0 0.011932233006174426 0.0 38-39 6.067237121783605E-4 0.0 0.0 0.01506697218576262 0.0 40-41 6.067237121783605E-4 0.0 0.0 0.020123003120582295 0.0 42-43 6.067237121783605E-4 0.0 0.0 0.029324979421954094 0.0 44-45 6.067237121783605E-4 0.0 0.0 0.041156091809432124 0.0 46-47 6.067237121783605E-4 0.0 0.0 0.05723427018215868 0.0 48-49 6.067237121783605E-4 0.0 0.0 0.09050295373327212 0.0 50-51 6.067237121783605E-4 0.0 0.0 0.11770440016260195 0.0 52-53 6.067237121783605E-4 0.0 0.0 0.14804058577152 0.0 54-55 6.067237121783605E-4 0.0 0.0 0.21194881678764063 0.0 56-57 6.067237121783605E-4 0.0 0.0 0.27322791171765504 0.0 58-59 6.067237121783605E-4 0.0 0.0 0.3258106334397797 0.0 60-61 6.067237121783605E-4 0.0 0.0 0.39083119126156063 0.0 62-63 6.067237121783605E-4 0.0 0.0 0.47536802849174553 0.0 64-65 6.067237121783605E-4 0.0 0.0 0.5374560883713311 0.0 66-67 8.089649495711474E-4 0.0 0.0 0.6323072287085482 0.0 68-69 8.089649495711474E-4 0.0 0.0 0.775190662926552 0.0 70-71 8.089649495711474E-4 0.0 0.0 0.8875356702982451 0.0 72-73 8.089649495711474E-4 0.0 0.0 1.0552947767155618 0.0 74-75 8.089649495711474E-4 0.0 0.0 1.2687604027836485 0.0 76-77 8.089649495711474E-4 0.0 0.0 1.4404632133301245 0.0 78-79 8.089649495711474E-4 0.0 0.0 1.615907486768367 0.0 80-81 8.089649495711474E-4 0.0 0.0 1.8505073221439998 0.0 82-83 8.089649495711474E-4 0.0 0.0 2.1086682616758923 0.0 84-85 8.089649495711474E-4 0.0 0.0 2.3842219476235647 0.0 86-87 8.089649495711474E-4 0.0 0.0 2.709223616113773 0.0 88-89 8.089649495711474E-4 0.0 0.0 3.111178075431937 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTCGTA 25 0.0050162156 57.009663 7 AGGACCG 105 6.871041E-5 27.158464 5 GGACCGA 95 0.0012243527 25.004238 6 GTGTAGC 230 1.3278623E-10 24.811947 1 CACCGTG 135 1.3389494E-5 24.633806 7 GAGTACT 2165 0.0 23.802858 12-13 GCACCGT 100 0.0016506837 23.754026 6 GTACTTT 2250 0.0 23.111223 14-15 GTACATG 2155 0.0 22.950573 1 GTATCAA 4310 0.0 22.619555 1 TAAGGCA 190 3.260957E-7 22.512938 4 GGTATCA 3340 0.0 22.211891 1 CTTATGC 150 3.0129158E-5 22.18166 3 GTAAGCG 65 2.9457916E-4 21.917913 94-95 TACATGG 2240 0.0 21.856293 2 ACATGGG 2130 0.0 21.422932 3 AATCGAG 95 8.255287E-6 19.998325 80-81 GTATTGA 120 0.0047272486 19.815096 1 CGGTTCA 60 0.0047639436 19.791012 34-35 ACTTTTT 2640 0.0 19.787003 16-17 >>END_MODULE