FastQCFastQC Report
Thu 26 May 2016
SRR937608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494459
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36680.7418208587567423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29440.5953982028843645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.36039388503394615No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16340.33046218189981375No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10570.2137689879241757No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9330.18869107448747016No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.1569392002168026No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6530.13206352801748983No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6300.12741197955745573No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5980.12094025996088655No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA5850.11831112387478031No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG5150.10415423725728523No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA5020.101525101171179No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5010.10132285993378623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT700.00838428427.1446785
GTATAGA1257.364335E-626.6071641
GTACATG18700.026.424231
TAAGGTG2101.2605597E-924.8826225
GGTATCA31550.024.8482151
ACATGGG19450.024.6674633
TACATGG19650.024.6581422
GGGTAGT1751.4830584E-724.427746
GTAGCCC2002.046545E-823.7515933
GTATCAA42000.023.4170231
GAGTACT28000.023.15780312-13
GTACTTT29100.022.28016914-15
GTGTAGC2858.367351E-1121.6725031
CATGGGG9750.021.4373364
GTGTAGG2005.3593976E-721.3807561
AGAGTAC37150.020.90651710-11
GTATATG3001.6552804E-1020.588881
AGTACTT29300.020.34692812-13
GAGTATA1656.3003245E-520.1569441
ATCAACG49450.020.077183
Produced by FastQC (version 0.11.5)