##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937608_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 494459 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23836758962826 33.0 31.0 34.0 31.0 34.0 2 32.45212848790294 34.0 31.0 34.0 31.0 34.0 3 32.55569420315941 34.0 31.0 34.0 31.0 34.0 4 36.169512537945515 37.0 35.0 37.0 35.0 37.0 5 36.01357847667855 37.0 35.0 37.0 35.0 37.0 6 36.11485279871536 37.0 35.0 37.0 35.0 37.0 7 36.08840571210151 37.0 35.0 37.0 35.0 37.0 8 36.08151130831879 37.0 35.0 37.0 35.0 37.0 9 37.74330126461446 39.0 37.0 39.0 35.0 39.0 10-11 37.69916312575967 39.0 37.0 39.0 35.0 39.0 12-13 37.61907458454594 39.0 37.0 39.0 35.0 39.0 14-15 38.84569398069405 40.0 38.0 41.0 35.0 41.0 16-17 38.817575370253145 40.0 38.0 41.0 35.0 41.0 18-19 38.82616051078047 40.0 38.0 41.0 35.0 41.0 20-21 38.756028305683586 40.0 38.0 41.0 35.0 41.0 22-23 38.61242691507284 40.0 38.0 41.0 34.0 41.0 24-25 38.55160387413314 40.0 38.0 41.0 34.0 41.0 26-27 38.48231501499619 40.0 38.0 41.0 34.0 41.0 28-29 38.37350012842319 40.0 38.0 41.0 34.0 41.0 30-31 38.421764999726975 40.0 38.0 41.0 34.0 41.0 32-33 38.31434962251673 40.0 38.0 41.0 34.0 41.0 34-35 38.17385162369378 40.0 38.0 41.0 34.0 41.0 36-37 37.99991303626792 40.0 37.0 41.0 33.5 41.0 38-39 37.85786485836035 40.0 37.0 41.0 33.0 41.0 40-41 37.6762775073363 40.0 37.0 41.0 33.0 41.0 42-43 37.46483530484833 40.0 37.0 41.0 33.0 41.0 44-45 37.14790811776103 40.0 36.5 41.0 32.0 41.0 46-47 36.73695493458507 40.0 36.0 41.0 31.0 41.0 48-49 36.352527712105555 39.0 36.0 41.0 30.0 41.0 50-51 36.07601439148645 39.0 35.5 41.0 29.5 41.0 52-53 35.87203792427684 39.0 35.0 40.5 29.0 41.0 54-55 35.66375169629838 39.0 35.0 40.0 28.5 41.0 56-57 35.376647002077014 38.5 35.0 40.0 28.0 41.0 58-59 35.105535544908676 38.0 35.0 40.0 27.5 41.0 60-61 34.74441460262631 38.0 34.0 40.0 26.5 41.0 62-63 34.40095943243019 37.5 34.0 40.0 26.0 41.0 64-65 34.00444425119171 37.0 34.0 39.5 26.0 41.0 66-67 33.57506588008308 36.0 33.0 39.0 24.5 40.5 68-69 33.11769529930692 36.0 33.0 39.0 23.0 40.0 70-71 32.683021241397164 35.0 32.0 38.5 22.5 40.0 72-73 32.22717555955094 35.0 32.0 37.5 21.5 39.5 74-75 31.73631989709966 35.0 31.0 37.0 20.0 39.0 76-77 30.51102416985028 34.0 29.5 35.5 19.5 38.0 78-79 31.083131260630307 34.0 31.0 36.0 19.5 38.5 80-81 30.865281044535543 34.0 31.0 36.0 18.0 37.5 82-83 30.50429661508841 34.0 31.0 35.0 16.5 37.0 84-85 30.096493339184846 34.0 30.0 35.0 10.0 36.5 86-87 29.68555431289551 34.0 30.0 35.0 7.0 36.0 88-89 29.243582379934434 34.0 29.5 35.0 2.0 36.0 90-91 28.726041997415358 34.0 29.0 35.0 2.0 35.0 92-93 28.278257651291614 33.5 29.0 35.0 2.0 35.0 94-95 27.774696183101128 33.0 27.0 35.0 2.0 35.0 96-97 27.205688439284145 33.0 26.5 35.0 2.0 35.0 98-99 26.372957110700785 32.5 25.0 34.5 2.0 35.0 100-101 23.913735011396295 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25455700339197307 1101 2 0.26719589452089565 1101 3 0.22615306099990562 1101 4 0.13274876881531128 1101 5 0.008937121900039813 1101 6 0.10789092586549032 1101 7 0.04624544515505846 1101 8 0.12943331915683842 1101 9 0.09513805703159761 1101 10-11 0.17211413215851934 1101 12-13 0.04317447621925652 1101 14-15 0.14157365457385396 1101 16-17 -0.06797920426568993 1101 18-19 0.09205474593398577 1101 20-21 0.29482769691180266 1101 22-23 0.10227466656369444 1101 24-25 0.05574921179839265 1101 26-27 0.06418981824283065 1101 28-29 0.18525304633769935 1101 30-31 0.15724030690171276 1101 32-33 0.30349038939311157 1101 34-35 0.1762187861956761 1101 36-37 0.24048731063695783 1101 38-39 0.2018247808203384 1101 40-41 0.20576004561260675 1101 42-43 0.15318399453251175 1101 44-45 0.1740507985248385 1101 46-47 -0.052788638014391154 1101 48-49 -0.018381773744366114 1101 50-51 0.004419846588227472 1101 52-53 0.05801493810796643 1101 54-55 0.22768607220866244 1101 56-57 0.1517589274465081 1101 58-59 0.45898327511076786 1101 60-61 0.3698614324937992 1101 62-63 0.22811045494736248 1101 64-65 0.052709072273103175 1101 66-67 0.23375708301670528 1101 68-69 0.09878217826919666 1101 70-71 0.23279779351822327 1101 72-73 0.28686921969357826 1101 74-75 0.47842174318119746 1101 76-77 0.38361936851319456 1101 78-79 0.07934819924773251 1101 80-81 0.1904065184153616 1101 82-83 0.1379195607727226 1101 84-85 0.17579922014049387 1101 86-87 0.45132440701762633 1101 88-89 0.46356889139247315 1101 90-91 0.5584919634516154 1101 92-93 0.861610120850056 1101 94-95 0.7609722936099494 1101 96-97 1.0542021633679504 1101 98-99 1.727703848538443 1101 100-101 2.100126008618492 1102 1 0.22677337454523894 1102 2 0.14634103269560939 1102 3 0.12849114815468 1102 4 0.04013804938163901 1102 5 -0.012942434189682217 1102 6 0.10744486790273555 1102 7 0.11317066023347166 1102 8 0.07124753601534906 1102 9 0.14223021343273246 1102 10-11 0.17962174198996905 1102 12-13 0.10864793783555626 1102 14-15 0.2920418089264203 1102 16-17 0.2682225295781322 1102 18-19 0.2530630847397859 1102 20-21 0.4186666039045903 1102 22-23 0.27449124422528115 1102 24-25 0.1376431821685813 1102 26-27 0.22959988180609514 1102 28-29 -0.005342771455886464 1102 30-31 0.24624126097013033 1102 32-33 0.2177915032989688 1102 34-35 0.23155165695037994 1102 36-37 0.24725665323838086 1102 38-39 0.2876899061089304 1102 40-41 0.12227183104219819 1102 42-43 0.26312179422770754 1102 44-45 0.4507089570488816 1102 46-47 0.35794710981417666 1102 48-49 0.7014876303965423 1102 50-51 0.8245841446045077 1102 52-53 0.8025631185631923 1102 54-55 0.7378586718592501 1102 56-57 0.7005589076217049 1102 58-59 0.683931751285904 1102 60-61 0.697312376443314 1102 62-63 0.9387045380710859 1102 64-65 0.8426312599395942 1102 66-67 1.0658740823682678 1102 68-69 0.8179711375085859 1102 70-71 0.7957590814692921 1102 72-73 0.9693461630400932 1102 74-75 0.9018928588630253 1102 76-77 0.844986825280678 1102 78-79 1.012779211014582 1102 80-81 1.0573015832796244 1102 82-83 0.9408176984807284 1102 84-85 0.7251010155237942 1102 86-87 0.9075250257165415 1102 88-89 0.7441951072101816 1102 90-91 0.8800212814372479 1102 92-93 1.2236491426614684 1102 94-95 1.034942746400084 1102 96-97 1.0972126829015743 1102 98-99 1.3193532717607184 1102 100-101 1.9605047035685352 1103 1 0.10745380356150491 1103 2 0.05493058407376594 1103 3 0.03351479017981518 1103 4 0.08227621107002392 1103 5 -0.08410377659540558 1103 6 -0.021225412341756567 1103 7 0.12599963036379336 1103 8 -0.06333209724494537 1103 9 0.08074941537022795 1103 10-11 -0.09933108928670009 1103 12-13 0.10464724587384211 1103 14-15 0.15008817036109434 1103 16-17 0.01981058170591865 1103 18-19 -0.03367316252120389 1103 20-21 0.04921210038711621 1103 22-23 0.029536798371282202 1103 24-25 -0.1449712798311964 1103 26-27 0.07858269809026552 1103 28-29 0.08809051190243622 1103 30-31 -0.036745707084307355 1103 32-33 0.13220621901803042 1103 34-35 0.00654300742899494 1103 36-37 0.09221225236189667 1103 38-39 0.055662458294378325 1103 40-41 0.03150490681201035 1103 42-43 0.11373072053378053 1103 44-45 -0.14067265983498345 1103 46-47 0.12463030667727537 1103 48-49 0.3040594214240997 1103 50-51 0.3490161593663572 1103 52-53 0.3794135395065652 1103 54-55 0.4977687168367595 1103 56-57 0.4878937017630989 1103 58-59 0.3213714929535314 1103 60-61 0.33056920956521907 1103 62-63 0.4916811266998522 1103 64-65 0.4470828802832756 1103 66-67 0.445287068183049 1103 68-69 0.35130177987971223 1103 70-71 0.3330881092630875 1103 72-73 0.4502491730735372 1103 74-75 0.34122742235051007 1103 76-77 0.24535702479630572 1103 78-79 0.5197910896906279 1103 80-81 0.40512997677518214 1103 82-83 0.5432914615991677 1103 84-85 0.6012283364786981 1103 86-87 0.7255414021437154 1103 88-89 1.0133082429500035 1103 90-91 0.6202380961068386 1103 92-93 0.8409447256392077 1103 94-95 0.8168421812980178 1103 96-97 1.0990336954722082 1103 98-99 1.5467884003502697 1103 100-101 2.74450193572169 1104 1 0.15603957341970442 1104 2 0.13564083630114254 1104 3 0.10366913827870405 1104 4 0.10501181152177708 1104 5 0.02815121148340438 1104 6 -0.007258791686716393 1104 7 -0.0408167832389168 1104 8 0.16915127582570477 1104 9 0.06680789196703785 1104 10-11 0.13660817536381842 1104 12-13 0.13619574631304232 1104 14-15 0.040139619727384 1104 16-17 0.12675248156787688 1104 18-19 0.20911890674721434 1104 20-21 0.07619917530241338 1104 22-23 0.27128607682529093 1104 24-25 0.09601880675432994 1104 26-27 0.12373641875785069 1104 28-29 0.007372730499497493 1104 30-31 0.28655960098446087 1104 32-33 -0.0258073334979656 1104 34-35 0.05509991544756332 1104 36-37 0.2208620202123157 1104 38-39 0.11859256244797223 1104 40-41 0.1927622791297381 1104 42-43 0.41093614154018354 1104 44-45 0.17391260807817588 1104 46-47 0.22106775177704208 1104 48-49 0.1849604127655482 1104 50-51 0.04400298330914154 1104 52-53 0.1665348847192618 1104 54-55 0.23587638404720224 1104 56-57 0.28595870364539167 1104 58-59 0.25111675584724225 1104 60-61 0.22900941536267538 1104 62-63 0.5359100136367303 1104 64-65 0.3769611586117918 1104 66-67 0.5342932170097541 1104 68-69 0.5808926946745387 1104 70-71 0.46798332823661326 1104 72-73 0.25926034135060405 1104 74-75 0.308057639441742 1104 76-77 0.10446230776781817 1104 78-79 0.29069082616948094 1104 80-81 0.4201531917331458 1104 82-83 0.4476144632304937 1104 84-85 0.5176419391892004 1104 86-87 0.28737099180364467 1104 88-89 0.7478771976494656 1104 90-91 0.6162375941617988 1104 92-93 0.777921761653765 1104 94-95 0.939046974873122 1104 96-97 1.2367787073934018 1104 98-99 1.503306156655956 1104 100-101 2.0290972425355953 1105 1 -5.910682333691852E-4 1105 2 0.013872891766069984 1105 3 0.04707248248750773 1105 4 0.057404416198231445 1105 5 -0.2125332637748869 1105 6 0.026210485094139813 1105 7 -0.018647805533646533 1105 8 -0.12509491775776382 1105 9 -0.07774417437336467 1105 10-11 -0.05582147390209258 1105 12-13 9.596382256660263E-5 1105 14-15 0.07231573446365758 1105 16-17 0.05437788939821786 1105 18-19 0.04542940158136588 1105 20-21 0.0788755619255781 1105 22-23 0.11110731119179462 1105 24-25 0.13515692529701084 1105 26-27 0.0071564160389741005 1105 28-29 0.09579884523576965 1105 30-31 -0.012835450674046456 1105 32-33 0.10959403953084745 1105 34-35 0.08402698178796442 1105 36-37 -0.02059223481759176 1105 38-39 -0.24563561862870387 1105 40-41 -0.11206881113670875 1105 42-43 -0.27155774100467767 1105 44-45 -0.026121377783702826 1105 46-47 -0.19875110357912718 1105 48-49 -0.2247867324220536 1105 50-51 -0.29898063550543696 1105 52-53 -0.5422691528011256 1105 54-55 -0.3947312831632388 1105 56-57 -0.3526832213138249 1105 58-59 -0.19899709679005895 1105 60-61 -0.4312577135117053 1105 62-63 -0.22216502714631048 1105 64-65 -0.2660581453577606 1105 66-67 -0.5209949830990013 1105 68-69 -0.5219995021715746 1105 70-71 -0.5190272753523004 1105 72-73 -0.4276194166700549 1105 74-75 -0.23406103918795296 1105 76-77 -0.5203320777677973 1105 78-79 -0.58381467954014 1105 80-81 -0.6750463052760978 1105 82-83 -0.4900418717341637 1105 84-85 -0.4096690994187391 1105 86-87 -0.14285603375372347 1105 88-89 -0.06380714166537871 1105 90-91 -0.07612408338033916 1105 92-93 0.25940626410074685 1105 94-95 0.0590697454005813 1105 96-97 0.31912984931836164 1105 98-99 0.6228140413759071 1105 100-101 1.4387968075165638 1106 1 -0.2570125775150487 1106 2 0.047513932928296754 1106 3 -0.20003708167713796 1106 4 -0.030932952647610534 1106 5 -0.05848261809288857 1106 6 -0.09287748907858173 1106 7 -0.36168150206310656 1106 8 -0.09654871113952623 1106 9 -0.09583840359047713 1106 10-11 -0.17062928261879762 1106 12-13 -0.27327974239212693 1106 14-15 -0.4711038579495792 1106 16-17 -0.3118675706502003 1106 18-19 -0.035664222308874116 1106 20-21 -0.3085714719727264 1106 22-23 -0.09888562666130696 1106 24-25 -0.04561385759241432 1106 26-27 -0.15445324901348556 1106 28-29 -0.22057776896923542 1106 30-31 -0.2714411811018067 1106 32-33 -0.49765222762007966 1106 34-35 -0.7834644427479489 1106 36-37 -0.569696855479414 1106 38-39 -0.536442216691654 1106 40-41 -0.703909509280372 1106 42-43 -0.48192396376497015 1106 44-45 -0.5879756957820916 1106 46-47 -0.7131402278729198 1106 48-49 -0.7522080399281066 1106 50-51 -0.8027160067725845 1106 52-53 -0.939068486941558 1106 54-55 -0.8509964163351498 1106 56-57 -0.8103406063017147 1106 58-59 -0.7850468344817472 1106 60-61 -0.6370839846981511 1106 62-63 -0.5267655114078096 1106 64-65 -0.6498252962859254 1106 66-67 -0.5983355803548491 1106 68-69 -0.9590707289673688 1106 70-71 -0.7416967668777232 1106 72-73 -0.4249585006087173 1106 74-75 -0.6673086178804475 1106 76-77 -0.7159560688896676 1106 78-79 -0.7322483962471651 1106 80-81 -0.34478248364575137 1106 82-83 -0.2984760928802501 1106 84-85 -0.17696127052446897 1106 86-87 -0.16611067459311002 1106 88-89 5.088389641585422E-4 1106 90-91 -0.03889395827945208 1106 92-93 -0.15863247681935277 1106 94-95 -0.4198220019763319 1106 96-97 -0.22501361315137203 1106 98-99 0.276345114822238 1106 100-101 1.0344888979120448 1107 1 0.12151653694462539 1107 2 0.05835104710588013 1107 3 0.055628307730231086 1107 4 0.08588338059952605 1107 5 -0.2382411925774761 1107 6 0.01876711939834763 1107 7 -0.11478453692522805 1107 8 0.09029747706424018 1107 9 0.036798705885537686 1107 10-11 0.0818302251270353 1107 12-13 0.05644709650864144 1107 14-15 0.17177973122741008 1107 16-17 0.02060968551472797 1107 18-19 -0.04470004825682139 1107 20-21 0.1439544454207251 1107 22-23 0.13904015908823197 1107 24-25 0.06120870523228916 1107 26-27 0.014994199210498493 1107 28-29 -0.13404541042766738 1107 30-31 -0.3220182985704838 1107 32-33 -0.22749131645620224 1107 34-35 0.13365279084945314 1107 36-37 0.04171561307884275 1107 38-39 -0.15106644387142154 1107 40-41 -0.10582892440531566 1107 42-43 -0.38994366333477615 1107 44-45 -0.2980064910523126 1107 46-47 -0.0846228673331808 1107 48-49 -0.20973818873272876 1107 50-51 0.17946798909002837 1107 52-53 0.24137769185906421 1107 54-55 0.24907939644840837 1107 56-57 0.3704963903366618 1107 58-59 0.34664408443970984 1107 60-61 0.3277835486242182 1107 62-63 0.12565050683427614 1107 64-65 0.06691111030568209 1107 66-67 -0.032969093131363536 1107 68-69 0.11281036837859659 1107 70-71 0.14031738484187883 1107 72-73 0.529550890773308 1107 74-75 0.4359025530450573 1107 76-77 0.20933013906068254 1107 78-79 0.20662828162490499 1107 80-81 0.41003662352649073 1107 82-83 0.3828545683952882 1107 84-85 0.23175484880132657 1107 86-87 0.38364073000032306 1107 88-89 0.8263627612472426 1107 90-91 0.551414347040371 1107 92-93 1.1669305359454079 1107 94-95 0.9573201499313271 1107 96-97 1.1706468396096277 1107 98-99 1.587538960469761 1107 100-101 2.504128522726919 1108 1 0.011803936187497754 1108 2 0.007026074789941106 1108 3 -0.16017221247270186 1108 4 -0.029288775667374978 1108 5 -0.3427775166484537 1108 6 -0.08780366167862752 1108 7 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-0.32800489501197205 2316 66-67 -0.8436436691294276 2316 68-69 -0.6083152006501322 2316 70-71 -0.38671399734400325 2316 72-73 -0.3571016089245518 2316 74-75 -0.4040039852460424 2316 76-77 -0.6486040348908944 2316 78-79 -0.5805600114903484 2316 80-81 -0.42880235645175446 2316 82-83 -0.47973758404398126 2316 84-85 -0.4372970385612014 2316 86-87 -0.390867790323572 2316 88-89 -0.7907262288023098 2316 90-91 -0.7925962479723196 2316 92-93 -0.9534568064386733 2316 94-95 -0.7878657663559885 2316 96-97 -0.27550189342022335 2316 98-99 -0.1543989600072173 2316 100-101 -0.6364971067296317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 1.0 5 11.0 6 26.0 7 45.0 8 108.0 9 212.0 10 373.0 11 586.0 12 939.0 13 1407.0 14 1905.0 15 2730.0 16 3772.0 17 5831.0 18 5294.0 19 3088.0 20 2472.0 21 2471.0 22 2557.0 23 2735.0 24 3090.0 25 3712.0 26 4372.0 27 5374.0 28 6599.0 29 8373.0 30 10304.0 31 13376.0 32 17523.0 33 23225.0 34 32639.0 35 48591.0 36 75982.0 37 114415.0 38 84499.0 39 5819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44260971775735 19.705800833921717 16.236387067537137 21.615202380783792 2 15.30784149949743 20.38551224671813 40.58334462513575 23.723301628648684 3 19.620231404423823 24.638240986613653 30.61871661755575 25.12281099140677 4 12.765466904232706 17.81441939574363 37.984140242163654 31.435973457860005 5 13.28259159100241 37.600804208746375 34.25856443884393 14.858039761407285 6 30.97688584897838 37.14807496678188 17.038824250342294 14.836214933897452 7 27.921425234448154 33.771455267271904 20.449824960209035 17.857294538070903 8 26.458412123148733 34.1559158595556 19.526593711510966 19.859078305784706 9 26.041390691644807 17.538966830414655 20.94349582068483 35.47614665725571 10-11 24.810247159016217 27.19426686540239 26.716471942061933 21.279014033519463 12-13 26.395577191836374 24.431424023687548 27.588145709160116 21.584853075315966 14-15 22.825047172768624 25.70546799633539 27.029844739402055 24.439640091493935 16-17 21.31248495830797 29.131839040243985 27.689050052683843 21.866625948764202 18-19 21.177084449873497 26.99404804038353 30.85493438283053 20.973933126912446 20-21 21.859974092871294 25.700539074563384 31.284627526880413 21.154859305684905 22-23 21.599407836459314 25.6714422660772 31.466171039805204 21.262978857658286 24-25 21.823390459857055 25.8577068224197 31.341590185617385 20.97731253210586 26-27 21.312274435767094 26.116760996208953 31.51392089666491 21.057043671359043 28-29 20.869042021225496 26.801361101420255 31.318175153326155 21.011421724028093 30-31 21.13693956425103 26.20257695784686 31.09661266151491 21.563870816387205 32-33 21.057964361049148 26.21794729188871 31.136049702806503 21.58803864425564 34-35 21.78016781977879 26.06990670611719 30.92349416230668 21.226431311797338 36-37 21.7433599145733 25.659963717922015 31.1473752121005 21.44930115540419 38-39 21.38084249654673 26.37660554262335 31.043524336699303 21.199027624130615 40-41 21.301159449013973 26.590071168691438 30.983256447956254 21.125512934338335 42-43 21.287730997594075 26.18018242147877 30.545433951349555 21.986652629577602 44-45 21.506333184348954 26.328067645649085 30.406363318293327 21.759235851708635 46-47 22.071698563480492 26.917701973267754 29.940702869196436 21.069896594055322 48-49 21.977049664380665 26.997890623893994 29.50365955519062 21.521400156534717 50-51 21.591678986528713 27.147043536471173 29.856772755678428 21.404504721321686 52-53 22.157752209991123 27.44322582863291 28.886520419286533 21.512501542089435 54-55 21.545567984403156 27.148155863276834 29.485963446918756 21.820312705401257 56-57 22.081203901637952 27.179503255072717 29.2126344145824 21.52665842870693 58-59 22.336634584465042 26.78280706792677 29.204747006324084 21.67581134128411 60-61 21.71575398566919 26.937926097007033 30.1463822076249 21.199937709698883 62-63 21.49824353485324 27.28760119645916 29.984386976473274 21.229768292214317 64-65 21.30935021912838 27.31257798927717 29.512254807779815 21.865816983814636 66-67 21.360315010951364 27.304690581018853 29.326698472471936 22.008295935557854 68-69 21.536366008101783 27.01285647546106 29.572118214048082 21.878659302389075 70-71 21.97694854376197 27.118830883854876 29.235083191933004 21.66913738045015 72-73 21.627981288640715 26.984340460988676 29.5855672563347 21.802110994035907 74-75 21.51877102044861 27.623928374243366 29.097761391743298 21.759539213564725 76-77 21.523220327671254 27.106494168373917 29.289789446647752 22.080496057307077 78-79 21.757617921809494 27.173334897332236 29.07773950924141 21.99130767161686 80-81 21.90151256221446 27.51704388028128 28.845566568714492 21.73587698878977 82-83 21.804828326831252 27.363147857144305 29.167753717714582 21.664270098309863 84-85 21.555846559107707 27.750817815484645 29.050727312256363 21.642608313151282 86-87 21.460525544079488 27.99493992424043 28.657178856083114 21.887355675596964 88-89 21.20570158496458 27.812821689968224 28.499632932154135 22.48184379291306 90-91 21.459726164820907 27.498601901414077 28.88395832300982 22.157713610755195 92-93 21.688463634810027 27.00447669580008 29.25020654283198 22.056853126557918 94-95 21.435144268786694 27.012451992986275 29.24832999298223 22.304073745244803 96-97 21.3958083481138 26.94126307742401 29.492637407752714 22.170291166709475 98-99 21.341528156559146 27.39390666759698 28.71332983455639 22.55123534128749 100-101 20.937563524161177 26.97874285621821 29.24290830170399 22.84078531791662 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153.0 1 119.5 2 118.5 3 155.5 4 120.0 5 94.0 6 213.0 7 536.5 8 981.5 9 1228.0 10 1032.5 11 774.0 12 745.0 13 790.5 14 865.5 15 924.5 16 945.5 17 1006.5 18 1086.0 19 1123.0 20 1207.0 21 1383.0 22 1574.5 23 1831.0 24 2101.5 25 2506.5 26 3155.5 27 3884.0 28 4502.0 29 5261.5 30 6300.0 31 7336.0 32 8638.0 33 9708.5 34 10378.5 35 11215.0 36 12552.5 37 13780.0 38 14415.0 39 14546.0 40 14903.0 41 15860.0 42 16495.0 43 17153.0 44 17868.0 45 18757.5 46 18974.5 47 20303.5 48 22975.5 49 21556.0 50 18316.0 51 16714.5 52 15558.5 53 14881.5 54 14279.5 55 13130.0 56 11640.5 57 10431.5 58 9600.5 59 8221.5 60 6472.0 61 5239.5 62 4278.0 63 3220.0 64 2218.0 65 1607.5 66 1223.0 67 950.0 68 691.5 69 450.5 70 307.5 71 227.5 72 185.5 73 153.5 74 119.5 75 97.5 76 69.0 77 39.0 78 29.5 79 20.5 80 9.5 81 8.5 82 6.0 83 2.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03397652788198819 2 0.0 3 0.0 4 0.0 5 0.01213447424356721 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004449307222641311 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0112061869639343E-4 22-23 2.0224123739278686E-4 24-25 2.0224123739278686E-4 26-27 9.100855682675409E-4 28-29 0.0013145680430531148 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011022147437906884 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.044824747855737E-4 84-85 3.0336185608918027E-4 86-87 0.0 88-89 0.0 90-91 0.007584046402229507 92-93 0.0019212917552314751 94-95 0.0 96-97 0.0 98-99 1.0112061869639343E-4 100-101 0.012235594862263605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 494459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.43316778478133 #Duplication Level Percentage of deduplicated Percentage of total 1 84.28256951548153 54.305929429235164 2 9.455933270055255 12.185514699023322 3 2.5787568133780314 4.984724112976042 4 1.1550552169231536 2.976954663707862 5 0.6090519427838469 1.9621573009519322 6 0.3882553899640747 1.500991481094056 7 0.2778542563802479 1.2532120944744913 8 0.18490024034222227 0.9530966567533441 9 0.1472097284792437 0.8536670221189692 >10 0.8295545360409957 9.743707355138866 >50 0.057934542144656494 2.5595904517139023 >100 0.028492397330926924 3.2980122661356774 >500 0.002849239733092688 1.1612805437351805 >1k 0.001582910962829271 2.261161922941239 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3668 0.7418208587567423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.5953982028843645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1782 0.36039388503394615 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1634 0.33046218189981375 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1057 0.2137689879241757 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 933 0.18869107448747016 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.1569392002168026 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 653 0.13206352801748983 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 630 0.12741197955745573 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 598 0.12094025996088655 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 585 0.11831112387478031 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 515 0.10415423725728523 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 502 0.101525101171179 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 501 0.10132285993378623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0224123739278686E-4 0.0 10-11 0.0 0.0 0.0 2.0224123739278686E-4 0.0 12-13 0.0 0.0 0.0 2.0224123739278686E-4 0.0 14-15 0.0 0.0 0.0 2.0224123739278686E-4 0.0 16-17 0.0 0.0 0.0 2.0224123739278686E-4 0.0 18-19 0.0 0.0 0.0 2.0224123739278686E-4 0.0 20-21 0.0 0.0 0.0 3.0336185608918027E-4 0.0 22-23 0.0 0.0 0.0 6.067237121783605E-4 0.0 24-25 0.0 0.0 0.0 6.067237121783605E-4 0.0 26-27 0.0 0.0 0.0 8.089649495711474E-4 0.0 28-29 0.0 0.0 0.0 0.001617929899142295 0.0 30-31 0.0 0.0 0.0 0.004247065985248524 0.0 32-33 0.0 0.0 0.0 0.006673960833961967 0.0 34-35 0.0 0.0 0.0 0.00940421753876459 0.0 36-37 0.0 0.0 0.0 0.012741197955745572 0.0 38-39 0.0 0.0 0.0 0.01597705775403016 0.0 40-41 0.0 0.0 0.0 0.021336450544939014 0.0 42-43 0.0 0.0 0.0 0.030032823752828848 0.0 44-45 0.0 0.0 0.0 0.04186393614030688 0.0 46-47 0.0 0.0 0.0 0.058043235131729834 0.0 48-49 0.0 0.0 0.0 0.09121079806414686 0.0 50-51 0.0 0.0 0.0 0.11831112387478032 0.0 52-53 0.0 0.0 0.0 0.14844506824630554 0.0 54-55 0.0 0.0 0.0 0.21215105802503342 0.0 56-57 0.0 0.0 0.0 0.2740368766672262 0.0 58-59 0.0 0.0 0.0 0.3279341664324039 0.0 60-61 0.0 0.0 0.0 0.39426929229723795 0.0 62-63 0.0 0.0 0.0 0.4786038882900301 0.0 64-65 0.0 0.0 0.0 0.5411975512630977 0.0 66-67 0.0 0.0 0.0 0.637666621499457 0.0 68-69 0.0 0.0 0.0 0.7814601412857285 0.0 70-71 0.0 0.0 0.0 0.8922883393769756 0.0 72-73 0.0 0.0 0.0 1.0606541695064706 0.0 74-75 0.0 0.0 0.0 1.2761422079484852 0.0 76-77 0.0 0.0 0.0 1.4500696721062818 0.0 78-79 0.0 0.0 0.0 1.6273341166810595 0.0 80-81 0.0 0.0 0.0 1.8651698118549769 0.0 82-83 0.0 0.0 0.0 2.1249486812860114 0.0 84-85 0.0 0.0 0.0 2.402322538370219 0.0 86-87 0.0 0.0 0.0 2.7314701522269793 0.0 88-89 0.0 0.0 0.0 3.137267195055606 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATT 70 0.008384284 27.144678 5 GTATAGA 125 7.364335E-6 26.607164 1 GTACATG 1870 0.0 26.42423 1 TAAGGTG 210 1.2605597E-9 24.882622 5 GGTATCA 3155 0.0 24.848215 1 ACATGGG 1945 0.0 24.667463 3 TACATGG 1965 0.0 24.658142 2 GGGTAGT 175 1.4830584E-7 24.42774 6 GTAGCCC 200 2.046545E-8 23.751593 3 GTATCAA 4200 0.0 23.417023 1 GAGTACT 2800 0.0 23.157803 12-13 GTACTTT 2910 0.0 22.280169 14-15 GTGTAGC 285 8.367351E-11 21.672503 1 CATGGGG 975 0.0 21.437336 4 GTGTAGG 200 5.3593976E-7 21.380756 1 AGAGTAC 3715 0.0 20.906517 10-11 GTATATG 300 1.6552804E-10 20.58888 1 AGTACTT 2930 0.0 20.346928 12-13 GAGTATA 165 6.3003245E-5 20.156944 1 ATCAACG 4945 0.0 20.07718 3 >>END_MODULE