FastQCFastQC Report
Thu 26 May 2016
SRR937607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602748
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75480.4709411585601729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.34752812045312176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.2352210079188993No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21850.13632835604848673No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21670.1352052849231445No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG20390.12721900136515535No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17530.10937464904027332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55050.039.137481
GTATCAA87350.036.916351
TATCAAC108600.029.6816982
ATCAACG110200.029.206693
TCAACGC111000.028.9132464
CAACGCA112100.028.5022855
AACGCAG115050.027.7901736
GTACATG95450.026.7577971
CGTATAG908.882158E-426.4228381
TACATGG100450.025.4636822
GTATAGG3800.025.0321641
GTACGGA950.001217604125.0321641
ACATGGG99650.024.4268763
ACGCAGA129700.024.3947897
CGCAGAG130250.024.145888
GAGTACT62150.023.54099712-13
GTACTTT65000.022.43538314-15
CATGGGG39100.022.3746954
GCAGAGT138050.022.162179
GGACCGA3900.021.9265696
Produced by FastQC (version 0.11.5)