##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1602748 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.711431241842135 33.0 31.0 34.0 30.0 34.0 2 31.83546072121132 33.0 31.0 34.0 30.0 34.0 3 31.860230678809145 33.0 31.0 34.0 30.0 34.0 4 35.46514361583979 37.0 35.0 37.0 33.0 37.0 5 35.457176050133896 37.0 35.0 37.0 33.0 37.0 6 35.53222527808489 37.0 35.0 37.0 33.0 37.0 7 35.46988422384555 37.0 35.0 37.0 33.0 37.0 8 35.44902504947752 37.0 35.0 37.0 33.0 37.0 9 37.0730326913526 39.0 37.0 39.0 33.0 39.0 10-11 36.93808446493148 39.0 37.0 39.0 33.0 39.0 12-13 36.822608732002784 39.0 37.0 39.0 33.0 39.0 14-15 37.95771200463204 40.0 38.0 41.0 33.0 41.0 16-17 37.87337248276086 40.0 38.0 41.0 33.0 41.0 18-19 37.809438227344536 40.0 38.0 41.0 33.0 41.0 20-21 37.710009309011774 40.0 37.5 41.0 32.0 41.0 22-23 37.621207451202565 40.0 37.0 41.0 32.0 41.0 24-25 37.6502067074799 40.0 37.0 41.0 32.0 41.0 26-27 37.565673143875394 40.0 37.0 41.0 32.0 41.0 28-29 37.43949329526538 40.0 37.0 41.0 32.0 41.0 30-31 37.19407324170737 39.5 36.5 41.0 31.5 41.0 32-33 37.04334305829738 39.0 36.0 41.0 31.0 41.0 34-35 37.0340780334775 39.5 36.0 41.0 31.0 41.0 36-37 36.892910176771395 39.5 36.0 41.0 31.0 41.0 38-39 36.73509123081108 39.0 36.0 41.0 30.0 41.0 40-41 36.489408815359624 39.0 35.5 41.0 30.0 41.0 42-43 36.20309493445008 39.0 35.0 41.0 30.0 41.0 44-45 35.925439931916934 39.0 35.0 41.0 29.0 41.0 46-47 35.539460039881504 39.0 35.0 40.0 27.0 41.0 48-49 35.1954458841939 39.0 35.0 40.0 26.0 41.0 50-51 34.2908273789766 37.5 33.5 39.5 24.5 40.5 52-53 34.368342995904534 38.0 34.0 39.5 24.0 40.5 54-55 34.633587126610045 38.0 34.0 40.0 24.0 41.0 56-57 34.401698520291404 38.0 34.0 40.0 23.5 41.0 58-59 34.14330106791586 38.0 34.0 40.0 23.0 41.0 60-61 33.81081960482871 37.5 33.0 40.0 22.0 41.0 62-63 33.42359591152196 37.0 33.0 40.0 21.5 41.0 64-65 33.01932462246092 36.0 32.5 39.0 20.0 41.0 66-67 32.569949237185135 36.0 32.0 39.0 18.5 40.5 68-69 32.05815293483442 35.0 31.0 39.0 15.5 40.0 70-71 31.597543718663196 35.0 31.0 38.0 12.0 40.0 72-73 31.13768540032494 35.0 30.5 37.0 8.0 39.5 74-75 30.597817311267896 34.5 30.0 37.0 7.0 39.0 76-77 30.109543109709072 34.0 29.5 36.0 2.0 39.0 78-79 29.556895407138242 34.0 29.0 36.0 2.0 37.5 80-81 28.999470908714283 34.0 29.0 35.0 2.0 37.0 82-83 28.49531398572951 34.0 27.5 35.0 2.0 36.5 84-85 27.985192307212365 33.5 26.5 35.0 2.0 36.0 86-87 27.4417668903658 33.0 26.0 35.0 2.0 36.0 88-89 26.970315046407794 33.0 25.5 35.0 2.0 35.0 90-91 26.419593410816923 32.0 24.5 35.0 2.0 35.0 92-93 25.794691055611985 32.0 23.5 34.0 2.0 35.0 94-95 25.037597613598642 31.5 19.0 34.0 2.0 35.0 96-97 24.176552708223625 31.0 9.0 34.0 2.0 35.0 98-99 23.10526764032774 31.0 2.0 34.0 2.0 35.0 100-101 20.280418381429897 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2080808124615281 1101 2 0.3112636092447012 1101 3 0.23058844432994263 1101 4 0.1158712345165398 1101 5 0.10682707061478425 1101 6 0.13250640806661806 1101 7 0.22942070445837714 1101 8 0.27661308455540734 1101 9 0.3163101937047017 1101 10-11 0.3320288639493967 1101 12-13 0.33081109744509263 1101 14-15 0.5284498961493185 1101 16-17 0.5573471275589625 1101 18-19 0.6270174908380852 1101 20-21 0.5495238590942648 1101 22-23 0.5732647968769626 1101 24-25 0.49756453460209826 1101 26-27 0.5179163574432906 1101 28-29 0.40574336277796164 1101 30-31 0.5507326285247771 1101 32-33 0.6196821830198687 1101 34-35 0.45682084440955606 1101 36-37 0.6501368726856 1101 38-39 0.5991168683665933 1101 40-41 0.6247261457097792 1101 42-43 0.7318923423190213 1101 44-45 0.7140937204941125 1101 46-47 0.6485042392544997 1101 48-49 0.7307964608437416 1101 50-51 0.811496414914906 1101 52-53 0.5991818434557032 1101 54-55 0.7030014503576254 1101 56-57 0.6378657504329652 1101 58-59 0.6464468672411741 1101 60-61 0.8381455928719319 1101 62-63 0.9007614062080407 1101 64-65 1.003780064616464 1101 66-67 0.9421604154925518 1101 68-69 0.826361289875301 1101 70-71 0.9803005514258878 1101 72-73 0.9164095541891513 1101 74-75 1.0082288580346166 1101 76-77 1.045072914186104 1101 78-79 1.0682568970051207 1101 80-81 1.207118716505395 1101 82-83 1.2339034944477625 1101 84-85 1.283830066981185 1101 86-87 1.3481991081188909 1101 88-89 1.2776682732030693 1101 90-91 1.1374793253088136 1101 92-93 1.2408277654914208 1101 94-95 1.2303759868558295 1101 96-97 1.4979078094323093 1101 98-99 1.7041559272657345 1101 100-101 1.9149391852152426 1102 1 0.19657051738315445 1102 2 0.3036155104192595 1102 3 0.34789751596833796 1102 4 0.17601943613286863 1102 5 0.15975112870762587 1102 6 0.1727492686684684 1102 7 0.16488949942939257 1102 8 0.12941196619055972 1102 9 0.2968932236145605 1102 10-11 0.29484643068352767 1102 12-13 0.3722739716610306 1102 14-15 0.4891378641333759 1102 16-17 0.43446930252275706 1102 18-19 0.4458024111865697 1102 20-21 0.4823637200296247 1102 22-23 0.5356179772269982 1102 24-25 0.5527351446317468 1102 26-27 0.34738125092908945 1102 28-29 0.519105264363688 1102 30-31 0.478839997032388 1102 32-33 0.5104883376813234 1102 34-35 0.47017178163402207 1102 36-37 0.6166380079538456 1102 38-39 0.5940074760206286 1102 40-41 0.5047335466536254 1102 42-43 0.4553432836551181 1102 44-45 0.4686606053820199 1102 46-47 0.6386443089695675 1102 48-49 0.8033604310389464 1102 50-51 0.8372101263055143 1102 52-53 0.7268899264741933 1102 54-55 0.7499490178683956 1102 56-57 0.8443842916246993 1102 58-59 0.805870050469359 1102 60-61 0.8917186040967024 1102 62-63 0.9368686467981107 1102 64-65 0.9337950035586857 1102 66-67 1.0207883627357006 1102 68-69 1.0221852387813755 1102 70-71 1.1439715109445565 1102 72-73 1.051746091552495 1102 74-75 0.9837214548065241 1102 76-77 1.0233061444239802 1102 78-79 1.0699721342693351 1102 80-81 1.1135267003877836 1102 82-83 1.3905364761601255 1102 84-85 1.5895239968366788 1102 86-87 1.628452521918451 1102 88-89 1.5381020050604768 1102 90-91 1.8088685098801314 1102 92-93 1.8390641022983232 1102 94-95 1.8505579495678681 1102 96-97 1.8704439118759915 1102 98-99 1.9960384167239589 1102 100-101 1.8341629084382198 1103 1 0.29617643120739245 1103 2 0.41934520274512366 1103 3 0.437302758073951 1103 4 0.3607353059072196 1103 5 0.32906725839320217 1103 6 0.10966088197456969 1103 7 0.20070619450626737 1103 8 0.24516528680961613 1103 9 0.4100022850650049 1103 10-11 0.33099286703059505 1103 12-13 0.29600141929904566 1103 14-15 0.40385639209070945 1103 16-17 0.4420035635478925 1103 18-19 0.38477770274283074 1103 20-21 0.38798873057797323 1103 22-23 0.3436368292294958 1103 24-25 0.43166904448153787 1103 26-27 0.5274737810129722 1103 28-29 0.275480262254014 1103 30-31 0.2885967943860166 1103 32-33 0.23908026018052198 1103 34-35 0.47921445601926393 1103 36-37 0.40146400245182434 1103 38-39 0.40715767601725616 1103 40-41 0.37716375880194875 1103 42-43 0.32841673389224724 1103 44-45 0.3299473347199253 1103 46-47 0.46626325210976916 1103 48-49 0.47164598740086916 1103 50-51 0.13717869223689405 1103 52-53 0.3939468115900624 1103 54-55 0.2624093560905365 1103 56-57 0.2203971437284622 1103 58-59 0.30928687043864755 1103 60-61 0.41598570507390065 1103 62-63 0.4440934193757826 1103 64-65 0.430998215318823 1103 66-67 0.6064565371124999 1103 68-69 0.5743683242989235 1103 70-71 0.4810653996552894 1103 72-73 0.3076666667326613 1103 74-75 0.31420448462880657 1103 76-77 0.36595626847252305 1103 78-79 0.2825738203168271 1103 80-81 0.4289224321053915 1103 82-83 0.46500483956327443 1103 84-85 0.3347483390418766 1103 86-87 0.38429503934431963 1103 88-89 0.45112077267621586 1103 90-91 0.7747922917572261 1103 92-93 0.48434245203081616 1103 94-95 0.5780108617550006 1103 96-97 0.8796338411598832 1103 98-99 1.0474360724597283 1103 100-101 0.9929178239670691 1104 1 0.3451837621545337 1104 2 0.31815683897602653 1104 3 0.33839665435913346 1104 4 0.25057168619714076 1104 5 0.24796971184387928 1104 6 0.31653537710586477 1104 7 0.337274658928024 1104 8 0.4113249723461223 1104 9 0.423277163474026 1104 10-11 0.432476336065875 1104 12-13 0.4664632622092455 1104 14-15 0.4378839093365201 1104 16-17 0.48935327744153767 1104 18-19 0.5012452103222031 1104 20-21 0.731796927786867 1104 22-23 0.4585242456198557 1104 24-25 0.42744356800377403 1104 26-27 0.5840902649974282 1104 28-29 0.5066615451866596 1104 30-31 0.6725031853901484 1104 32-33 0.6436410624274558 1104 34-35 0.5083377757993048 1104 36-37 0.38575138977526535 1104 38-39 0.5050745328018493 1104 40-41 0.5865944950399395 1104 42-43 0.3306385786951793 1104 44-45 0.34166535857507796 1104 46-47 0.45819295748614053 1104 48-49 0.4609189004130485 1104 50-51 0.38763391904334554 1104 52-53 0.31347602046290746 1104 54-55 0.5583872635428193 1104 56-57 0.5642416745850198 1104 58-59 0.5889351269305365 1104 60-61 0.7240418042258909 1104 62-63 0.8081950150143982 1104 64-65 0.9259408673033107 1104 66-67 0.9606272173797805 1104 68-69 0.8533701798498612 1104 70-71 0.8162230200886604 1104 72-73 0.7340116031741246 1104 74-75 0.6857920791385403 1104 76-77 0.7651990499061974 1104 78-79 0.9370799108501373 1104 80-81 1.140420806385901 1104 82-83 1.1927428756535683 1104 84-85 1.3740602896010756 1104 86-87 1.3598403820466665 1104 88-89 1.354844633574114 1104 90-91 1.3117841699195232 1104 92-93 1.3650031075912175 1104 94-95 1.5102031918064789 1104 96-97 1.7109977640446736 1104 98-99 1.343163446736856 1104 100-101 1.1904552053757342 1105 1 0.24693878228491428 1105 2 0.22138143917296205 1105 3 0.3182060285930781 1105 4 0.28904460263009923 1105 5 0.22810524393823073 1105 6 0.19445511318806297 1105 7 0.16924436156346445 1105 8 0.15837937215333397 1105 9 0.33570523216246073 1105 10-11 0.3820972035211412 1105 12-13 0.29783881740470974 1105 14-15 0.3262496299376423 1105 16-17 0.34233564829757057 1105 18-19 0.48997967627505545 1105 20-21 0.4306634772859965 1105 22-23 0.47063841333562806 1105 24-25 0.39764582742599686 1105 26-27 0.42616400575802516 1105 28-29 0.4470249307467995 1105 30-31 0.3875984774987913 1105 32-33 0.452428004479394 1105 34-35 0.5143448972588303 1105 36-37 0.43008434501477666 1105 38-39 0.5021912557670944 1105 40-41 0.5847497295619846 1105 42-43 0.3771684252555687 1105 44-45 0.2869504039255588 1105 46-47 0.4498281509579911 1105 48-49 0.36664078955261203 1105 50-51 0.21412458511171195 1105 52-53 0.40801194145375774 1105 54-55 0.4915878485495213 1105 56-57 0.4456747748297474 1105 58-59 0.4479539111303694 1105 60-61 0.47203666048790893 1105 62-63 0.6231691924525862 1105 64-65 0.5119522267375842 1105 66-67 0.5263444946056524 1105 68-69 0.6326221773070699 1105 70-71 0.6765655481652466 1105 72-73 0.6197537265656692 1105 74-75 0.4943586049548685 1105 76-77 0.6130310349818764 1105 78-79 0.5267658436823908 1105 80-81 0.5702135611110357 1105 82-83 0.7216242580643133 1105 84-85 0.5927740642654662 1105 86-87 0.8467277973391028 1105 88-89 0.7408645105066505 1105 90-91 0.9886647712133545 1105 92-93 1.1128675007090791 1105 94-95 1.2353460157103164 1105 96-97 1.5164894729872174 1105 98-99 1.5419003640672635 1105 100-101 1.5905759045584702 1106 1 0.1713234905412797 1106 2 0.2561466492189943 1106 3 0.021131784029275735 1106 4 0.22897361317185982 1106 5 0.17851927800805356 1106 6 0.19278020565780452 1106 7 0.2349467263144689 1106 8 0.20618718867289232 1106 9 0.2370661478582079 1106 10-11 0.14797405558235255 1106 12-13 0.0010320631673153002 1106 14-15 0.20345898484654157 1106 16-17 0.14503849512842493 1106 18-19 0.25549432851629916 1106 20-21 0.22349115293584987 1106 22-23 0.16262500895595622 1106 24-25 -5.21202578148916E-4 1106 26-27 0.18286235602103318 1106 28-29 0.30135533036113316 1106 30-31 0.17113673032194043 1106 32-33 0.2808069363098866 1106 34-35 0.40297677169622403 1106 36-37 0.4314853629285196 1106 38-39 0.357558553688186 1106 40-41 0.3961263875113943 1106 42-43 0.38359608554711855 1106 44-45 0.4118233780169689 1106 46-47 0.5487110482152602 1106 48-49 0.7425967368395234 1106 50-51 0.7627246337463163 1106 52-53 0.563772795716325 1106 54-55 0.5245990131776637 1106 56-57 0.5887705807350159 1106 58-59 0.695162754096053 1106 60-61 0.6509153768756377 1106 62-63 0.7195945489996944 1106 64-65 0.8803132832177241 1106 66-67 0.8153176645153408 1106 68-69 0.5910645474334828 1106 70-71 0.5966123909688186 1106 72-73 0.8736976090576434 1106 74-75 0.7042570268899269 1106 76-77 0.6497350166201805 1106 78-79 0.8394300388010123 1106 80-81 0.6920946537684891 1106 82-83 0.5944417801738595 1106 84-85 0.5785239117490519 1106 86-87 0.9352638957345896 1106 88-89 1.0835778521732813 1106 90-91 1.1018114728059238 1106 92-93 1.2269103162260784 1106 94-95 1.4141609525066592 1106 96-97 1.4072491113846652 1106 98-99 1.5094316459303947 1106 100-101 1.6544026373795724 1107 1 0.2501234956607128 1107 2 0.1691521398099063 1107 3 0.24877927700072533 1107 4 0.18968154530525538 1107 5 0.23001607196371054 1107 6 0.22311753357023179 1107 7 0.27370296028957597 1107 8 0.20105453138900486 1107 9 0.327424977385391 1107 10-11 0.3231800060689096 1107 12-13 0.4083483715448466 1107 14-15 0.4058674643325517 1107 16-17 0.4716350113548913 1107 18-19 0.5606803132177305 1107 20-21 0.5137844963942797 1107 22-23 0.37732631142480244 1107 24-25 0.4364993306107081 1107 26-27 0.4895398019363739 1107 28-29 0.4779166504920198 1107 30-31 0.4493819169892319 1107 32-33 0.471217813396585 1107 34-35 0.594918145666476 1107 36-37 0.4714856189001253 1107 38-39 0.5464587716906664 1107 40-41 0.4449408123645284 1107 42-43 0.5695251131536523 1107 44-45 0.7296255631612283 1107 46-47 0.6093822910853817 1107 48-49 0.5519911080239481 1107 50-51 0.4494749035830452 1107 52-53 0.40323487139006176 1107 54-55 0.6613330714794614 1107 56-57 0.6402607620908896 1107 58-59 0.6243870321494782 1107 60-61 0.5210812464751697 1107 62-63 0.5868634599685052 1107 64-65 0.7202324815146568 1107 66-67 0.7769814372808099 1107 68-69 0.6202017951414689 1107 70-71 0.7966292424327612 1107 72-73 0.6831295227440108 1107 74-75 0.5876707068656977 1107 76-77 0.7773622451729629 1107 78-79 0.8121161621537283 1107 80-81 0.8450543254422449 1107 82-83 0.8060191625229116 1107 84-85 0.730672153437439 1107 86-87 0.9273010457467485 1107 88-89 0.8660237461754399 1107 90-91 0.9450341979649437 1107 92-93 0.9176445707727723 1107 94-95 1.0522249966020354 1107 96-97 1.1422856513311679 1107 98-99 1.4610086443220425 1107 100-101 1.4991746306731208 1108 1 0.1020644903926744 1108 2 0.09538583662843436 1108 3 0.11568619752663523 1108 4 0.11849662136989991 1108 5 0.10664072119982393 1108 6 0.1994129810295462 1108 7 0.24304427329041545 1108 8 0.10253330739007538 1108 9 0.21476670340690873 1108 10-11 0.15546546814591977 1108 12-13 0.3166786342178938 1108 14-15 0.3323507957087628 1108 16-17 0.29263488582899555 1108 18-19 0.4214016416866002 1108 20-21 0.31437831485143874 1108 22-23 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-1.00191052529971 2316 94-95 -0.784890987862088 2316 96-97 -0.9603594841474958 2316 98-99 -1.1483932526978897 2316 100-101 -1.018468575212669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6011.0 3 1222.0 4 1140.0 5 1378.0 6 1556.0 7 1860.0 8 2274.0 9 2826.0 10 3555.0 11 4640.0 12 6162.0 13 7978.0 14 9141.0 15 11236.0 16 14441.0 17 18752.0 18 16053.0 19 11397.0 20 11420.0 21 12183.0 22 13202.0 23 14694.0 24 16652.0 25 18831.0 26 21751.0 27 25647.0 28 30430.0 29 35983.0 30 44238.0 31 54103.0 32 68871.0 33 89795.0 34 120211.0 35 166487.0 36 233225.0 37 294042.0 38 193104.0 39 16257.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46058847170386 18.675043476192325 14.440481124008247 22.423886928095573 2 15.374468497839574 19.841620960348923 40.72919436944054 24.05471617237096 3 19.216557420646716 24.751341457691282 29.424150931589573 26.607950190072433 4 12.565884696227982 16.248544326015907 36.67040662504529 34.515164352710826 5 13.691307256500487 37.84591083778339 33.314767519505054 15.148014386211065 6 32.192032609782935 35.74841202360709 17.29987746323897 14.75967790337101 7 29.61754967715476 33.05406344871737 19.607659259481547 17.72072761464632 8 27.20811605148368 34.69346925939843 18.997409810709243 19.101004878408652 9 27.407241620971778 16.909098070155586 19.92049058521755 35.76316972365508 10-11 25.579652719488692 26.79064852892676 26.31863588690328 21.31106286468127 12-13 26.280106288839704 24.084624721063687 27.4749961474828 22.16027284261381 14-15 23.049940015466568 25.299125582418874 26.07254783915018 25.57838656296438 16-17 22.195233727657122 29.180710583095284 26.273643327975233 22.350412361272365 18-19 22.145879575752858 27.01069932760748 29.337331866704304 21.50608922993536 20-21 23.076836523368762 26.0104418207925 29.29370183925822 21.61901981658052 22-23 22.63274789901258 25.94047473189125 29.333778826847627 22.092998542248537 24-25 22.41584579295385 26.314812296930455 29.4373987094193 21.831943200696394 26-27 22.066078402936483 26.675745247965914 29.364876454615015 21.893299894482592 28-29 21.808517289412283 26.652041043441727 29.70826070538101 21.83118096176498 30-31 21.869973724644908 26.295706378768386 29.68824392782952 22.14607596875718 32-33 21.894806015354455 26.474068530122256 29.433382072172876 22.197743382350414 34-35 22.42759636597704 26.564093874862486 29.007033643254637 22.00127611590584 36-37 22.58077924870439 26.108607014650996 29.09746890749789 22.213144829146724 38-39 22.043157427954547 26.467714117587132 29.246999727184004 22.24212872727432 40-41 22.120516041928408 26.427709751417304 29.26794052016726 22.183833686487027 42-43 22.36721068597068 26.296277195104604 28.851995805378305 22.48451631354641 44-45 22.131590792583054 26.693444529188916 28.662367621500646 22.51259705672739 46-47 22.345071538626733 26.97889773841598 28.17269300393462 22.50333771902267 48-49 22.584747113724895 26.84562499648339 28.116980181338164 22.45264770845355 50-51 22.58320342282284 27.063607749554002 27.8005887470354 22.55260008058776 52-53 22.772015929717128 27.194588699106454 27.598178638969774 22.435216732206644 54-55 22.394950852198406 27.103694375656172 27.639945977894193 22.86140879425123 56-57 22.508534471012798 27.55405998317481 27.635654651114827 22.301750894697562 58-59 22.62528988652667 26.85771911452616 27.817356409879 22.69963458906817 60-61 22.62932199560586 26.937904255741334 28.278676358232108 22.1540973904207 62-63 22.348938925562916 27.15602425527287 28.11547254906618 22.379564270098037 64-65 22.321268976206696 27.04027424485791 27.906789247701607 22.73166753123379 66-67 22.353391898243054 27.378928632141037 27.64107701768947 22.62660245192644 68-69 22.144032232457725 27.159977198261366 27.93147352485627 22.764517044424636 70-71 22.381703731273554 27.29608841129854 27.82756496736046 22.49464289006745 72-73 22.50107339926015 27.09615969931071 27.740355499835317 22.66241140159382 74-75 22.194555019503504 27.67938003798824 27.42953144717179 22.69653349533647 76-77 22.39791909783403 27.41194199825862 27.465914695012074 22.724224208895276 78-79 22.54485402654097 27.29142529964931 27.45294261228625 22.71077806152347 80-81 22.266847166441043 27.8990588761764 27.384247019691227 22.44984693769133 82-83 22.50218668526638 27.720794275337656 27.21042709033518 22.56659194906078 84-85 22.4573157013224 27.370072244581657 27.217302452316076 22.955309601779867 86-87 22.554346552445505 27.17057338577358 27.48932066926618 22.785759392514734 88-89 22.548553943687676 27.20011424457338 27.347263504983516 22.90406830675543 90-91 22.45375332900404 27.370835268286005 27.562309642845754 22.613101759864197 92-93 22.57832879407342 26.95620437043861 27.633406119813962 22.832060715674007 94-95 22.351033474363216 27.019363249918516 27.55167059319113 23.077932682527138 96-97 22.47874665829189 27.063531124954494 27.460770573281646 22.99695164347197 98-99 22.463056373191648 27.176515331679347 27.217266057551647 23.143162237577357 100-101 22.650591794454257 26.464458121360746 27.910303062871776 22.974647021313224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2185.0 1 1269.0 2 367.0 3 402.0 4 379.5 5 359.5 6 578.0 7 1182.0 8 1925.0 9 2225.0 10 2007.0 11 1835.5 12 1950.0 13 2177.0 14 2308.5 15 2403.0 16 2542.0 17 2683.0 18 2799.0 19 2900.0 20 3098.0 21 3383.5 22 3966.0 23 4806.5 24 5681.0 25 6909.5 26 8735.5 27 10675.0 28 12127.0 29 14083.0 30 16735.5 31 19207.5 32 22290.0 33 25517.5 34 29122.5 35 32452.5 36 36171.0 37 40019.5 38 41907.5 39 42777.5 40 44391.5 41 48903.5 42 53239.5 43 56323.5 44 59223.5 45 61606.5 46 61854.0 47 61698.0 48 64623.5 49 64249.0 50 60416.5 51 57197.0 52 55034.5 53 54776.0 54 54134.5 55 50112.5 56 44009.5 57 39054.5 58 35460.0 59 31541.5 60 26987.0 61 22756.5 62 18693.0 63 14640.0 64 11432.5 65 9014.5 66 6919.0 67 5718.0 68 4223.0 69 2657.0 70 2029.0 71 1622.0 72 1323.0 73 1077.0 74 789.5 75 645.5 76 447.5 77 273.5 78 192.0 79 111.5 80 73.0 81 55.5 82 46.5 83 29.5 84 16.0 85 19.0 86 13.0 87 7.0 88 7.5 89 8.0 90 6.0 91 5.0 92 3.5 93 2.0 94 2.5 95 1.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33205469605951776 2 0.2921856711098688 3 0.29156174270690094 4 0.29268481383224315 5 0.30129502579320017 6 0.20140408847803895 7 0.20090494575566464 8 0.20283912380486516 9 0.19897076770646416 10-11 0.20717542620549206 12-13 0.19619428631325697 14-15 0.19772291090052835 16-17 0.20595876581970465 18-19 0.1972549645983024 20-21 0.18886312757838414 22-23 0.18867594905749374 24-25 0.1922011445342624 26-27 0.18792723497393227 28-29 0.20414937345109774 30-31 0.20842328301142787 32-33 0.2126036033113128 34-35 0.20985831833825402 36-37 0.18249905786811152 38-39 0.17273457836166384 40-41 0.1795665943741624 42-43 0.1922011445342624 44-45 0.19766051806023155 46-47 0.20137289205789058 48-49 0.1993451247482449 50-51 0.20274553454442 52-53 0.23235093726524692 54-55 0.18920628820001648 56-57 0.17257859626092187 58-59 0.17273457836166384 60-61 0.16612093729020408 62-63 0.17257859626092187 64-65 0.16958373992667594 66-67 0.1684606688013337 68-69 0.17906745165178806 70-71 0.1729217568825542 72-73 0.1678991332386626 74-75 0.17338970318478014 76-77 0.1787554874503041 78-79 0.18215589724647915 80-81 0.17133073945498606 82-83 0.16992690054830828 84-85 0.1639683842999648 86-87 0.1608175458649769 88-89 0.16671366927302358 90-91 0.17438798862952878 92-93 0.16434274134174554 94-95 0.1704884361109794 96-97 0.16880382942296607 98-99 0.17323372108403817 100-101 0.1499611916533354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1602748.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.792881263501734 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89716840212601 35.317585951518005 2 13.602437718721157 13.001993815700356 3 4.961435159222805 7.11363844383895 4 2.3873678581247106 4.56396754302619 5 1.2963964668229293 3.0979261204645714 6 0.7939390777137454 2.276678164297627 7 0.5475692238378816 1.8318937628902177 8 0.38225091287781127 1.4615097993627504 9 0.2812021020944163 1.209551280880098 >10 1.606611573140737 14.305139979631342 >50 0.1478444466722161 4.8974985559292366 >100 0.09092933545314452 7.897915941028975 >500 0.0031444690978326023 0.9881122913587257 >1k 0.0014412150031732761 1.2151652171170741 >5k 2.6203909148605017E-4 0.8214231329558785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7548 0.4709411585601729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5570 0.34752812045312176 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.2352210079188993 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2185 0.13632835604848673 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2167 0.1352052849231445 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2039 0.12721900136515535 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1753 0.10937464904027332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 9.35892604451854E-5 0.0 26-27 0.0 0.0 0.0 3.431606216323464E-4 0.0 28-29 0.0 0.0 0.0 6.239284029679026E-4 0.0 30-31 0.0 0.0 0.0 0.001185463965639015 0.0 32-33 0.0 0.0 0.0 0.0019653744693488933 0.0 34-35 0.0 0.0 0.0 0.002838874233503957 0.0 36-37 0.0 0.0 0.0 0.004086731039439762 0.0 38-39 0.0 0.0 0.0 0.005584159206562729 0.0 40-41 0.0 0.0 0.0 0.007892694297543969 0.0 42-43 0.0 0.0 0.0 0.01160506829520299 0.0 44-45 0.0 0.0 0.0 0.018811441349482264 0.0 46-47 0.0 0.0 0.0 0.03403529438189909 0.0 48-49 0.0 0.0 0.0 0.046482666021108746 0.0 50-51 0.0 0.0 0.0 0.06139455485204162 0.0 52-53 0.0 0.0 0.0 0.08242094203205994 0.0 54-55 0.0 0.0 0.0 0.10684773900825333 0.0 56-57 0.0 0.0 0.0 0.13233521426949213 0.0 58-59 0.0 0.0 0.0 0.16571538382827494 0.0 60-61 0.0 0.0 0.0 0.20839208659127947 0.0 62-63 0.0 3.119642014839513E-5 0.0 0.2596790013152411 0.0 64-65 0.0 6.239284029679026E-5 0.0 0.30447706064833646 0.0 66-67 0.0 6.239284029679026E-5 0.0 0.3707694534636761 0.0 68-69 0.0 6.239284029679026E-5 0.0 0.45496859144419455 0.0 70-71 0.0 6.239284029679026E-5 0.0 0.5220096983430957 0.0 72-73 0.0 6.239284029679026E-5 0.0 0.622056617758999 0.0 74-75 0.0 6.239284029679026E-5 0.0 0.7108728259214798 0.0 76-77 0.0 6.239284029679026E-5 0.0 0.796943749110902 0.0 78-79 0.0 6.239284029679026E-5 0.0 0.9056944697482074 0.0 80-81 0.0 6.239284029679026E-5 0.0 1.0265805978232385 0.0 82-83 0.0 6.239284029679026E-5 0.0 1.1626905789306865 0.0 84-85 0.0 6.239284029679026E-5 0.0 1.3424755482458877 0.0 86-87 0.0 6.239284029679026E-5 0.0 1.568836772842643 0.0 88-89 0.0 6.239284029679026E-5 0.0 1.8335383978017754 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5505 0.0 39.13748 1 GTATCAA 8735 0.0 36.91635 1 TATCAAC 10860 0.0 29.681698 2 ATCAACG 11020 0.0 29.20669 3 TCAACGC 11100 0.0 28.913246 4 CAACGCA 11210 0.0 28.502285 5 AACGCAG 11505 0.0 27.790173 6 GTACATG 9545 0.0 26.757797 1 CGTATAG 90 8.882158E-4 26.422838 1 TACATGG 10045 0.0 25.463682 2 GTATAGG 380 0.0 25.032164 1 GTACGGA 95 0.0012176041 25.032164 1 ACATGGG 9965 0.0 24.426876 3 ACGCAGA 12970 0.0 24.394789 7 CGCAGAG 13025 0.0 24.14588 8 GAGTACT 6215 0.0 23.540997 12-13 GTACTTT 6500 0.0 22.435383 14-15 CATGGGG 3910 0.0 22.374695 4 GCAGAGT 13805 0.0 22.16217 9 GGACCGA 390 0.0 21.926569 6 >>END_MODULE