FastQCFastQC Report
Thu 26 May 2016
SRR937607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602748
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91350.569958596111179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76920.4799257275629107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51940.3240684125015286No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG25850.16128549216720284No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG22140.13813774841709364No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.1159258972714363No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17220.10744047099107282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59650.036.253741
GTATCAA92350.034.9449731
GTACATG88200.027.2667731
ATCAACG117800.027.1473053
TATCAAC119600.026.9373872
TCAACGC118900.026.8162254
CAACGCA120700.026.4950495
AACGCAG123550.025.9110136
TACATGG90900.025.8237922
ACATGGG90250.024.8514483
CATGGGG32900.023.975584
ACGCAGA140350.022.7438897
CGCAGAG140350.022.7438898
AGTACTT77900.022.13487212-13
GAGTACT76250.021.77284612-13
GCAGAGT148050.021.2080579
AGAGTAC128400.020.661710-11
GTACTTT79550.020.65870714-15
GGACCGA3503.6379788E-1220.3559076
CATGGGA55750.020.2004224
Produced by FastQC (version 0.11.5)