##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1602748 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21942314075575 33.0 31.0 34.0 31.0 34.0 2 32.49327717145802 34.0 31.0 34.0 31.0 34.0 3 32.57925263360179 34.0 31.0 34.0 31.0 34.0 4 36.19638895197498 37.0 35.0 37.0 35.0 37.0 5 36.114804697931305 37.0 35.0 37.0 35.0 37.0 6 36.16053553022684 37.0 36.0 37.0 35.0 37.0 7 36.10714847249849 37.0 35.0 37.0 35.0 37.0 8 36.08080683925358 37.0 35.0 37.0 35.0 37.0 9 37.790609004035566 39.0 38.0 39.0 35.0 39.0 10-11 37.70390136191092 39.0 37.0 39.0 35.0 39.0 12-13 37.602379475750396 39.0 37.0 39.0 35.0 39.0 14-15 38.84813426689661 40.0 38.0 41.0 35.0 41.0 16-17 38.805270697576915 40.0 38.0 41.0 35.0 41.0 18-19 38.787827531215136 40.0 38.0 41.0 35.0 41.0 20-21 38.69532577797632 40.0 38.0 41.0 34.5 41.0 22-23 38.64253862740743 40.0 38.0 41.0 34.0 41.0 24-25 38.658414485620945 40.0 38.0 41.0 34.0 41.0 26-27 38.53026551897116 40.0 38.0 41.0 34.0 41.0 28-29 38.37796677955612 40.0 38.0 41.0 34.0 41.0 30-31 38.419651124194196 40.0 38.0 41.0 34.0 41.0 32-33 38.33881433637727 40.0 38.0 41.0 34.0 41.0 34-35 38.18952885918435 40.0 38.0 41.0 34.0 41.0 36-37 38.04269448472249 40.0 37.0 41.0 33.5 41.0 38-39 37.90469836805287 40.0 37.0 41.0 33.0 41.0 40-41 37.71656648456276 40.0 37.0 41.0 33.0 41.0 42-43 37.48715830561011 40.0 37.0 41.0 33.0 41.0 44-45 37.157646117792694 40.0 36.5 41.0 32.0 41.0 46-47 36.7526919390946 40.0 36.0 41.0 31.0 41.0 48-49 36.394420395470775 39.0 36.0 41.0 30.0 41.0 50-51 36.12534472044264 39.0 35.5 41.0 30.0 41.0 52-53 35.88034519462822 39.0 35.0 40.5 29.0 41.0 54-55 35.64518657954963 39.0 35.0 40.0 28.5 41.0 56-57 35.370109337213336 38.0 35.0 40.0 28.0 41.0 58-59 35.104754771180495 38.0 34.5 40.0 27.5 41.0 60-61 34.77566654271289 38.0 34.0 40.0 27.0 41.0 62-63 34.41367669777158 37.0 34.0 40.0 26.0 41.0 64-65 34.00671128586653 37.0 33.5 39.5 26.0 41.0 66-67 33.57776082079029 36.0 33.0 39.0 25.0 40.5 68-69 33.13738934629774 35.5 33.0 39.0 24.0 40.0 70-71 32.74160067583925 35.0 32.0 38.0 23.5 40.0 72-73 32.27071255119333 35.0 32.0 37.5 22.0 39.5 74-75 31.803398288439606 35.0 31.0 37.0 20.5 39.0 76-77 30.573211134875848 33.5 29.5 35.5 20.0 38.0 78-79 31.16358061279752 34.0 31.0 36.0 20.0 38.0 80-81 30.981361074853936 34.0 31.0 35.5 19.5 37.0 82-83 30.628306196607326 34.0 31.0 35.0 18.0 37.0 84-85 30.2760683526044 34.0 31.0 35.0 17.0 36.0 86-87 29.906621003426615 34.0 30.0 35.0 10.0 36.0 88-89 29.49477085605473 34.0 30.0 35.0 7.0 36.0 90-91 28.979796574383496 34.0 29.0 35.0 2.0 35.0 92-93 28.55840219423141 34.0 29.0 35.0 2.0 35.0 94-95 28.104165782768096 33.0 29.0 35.0 2.0 35.0 96-97 27.549481265925774 33.0 27.0 35.0 2.0 35.0 98-99 26.67556814920374 33.0 25.5 34.5 2.0 35.0 100-101 23.85417295794473 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3247361148383803 1101 2 0.2710655720728852 1101 3 0.25423088527136883 1101 4 0.17971792333738534 1101 5 0.11141436832971863 1101 6 0.14955243566756593 1101 7 0.1898308026203992 1101 8 0.22342467206243555 1101 9 0.20376759164610547 1101 10-11 0.27752955687591907 1101 12-13 0.3109495790141992 1101 14-15 0.41463745666454344 1101 16-17 0.28751305639117675 1101 18-19 0.3044767832320261 1101 20-21 0.3544920208702962 1101 22-23 0.2360213633731405 1101 24-25 0.31112431963720155 1101 26-27 0.2003115610635291 1101 28-29 0.15382620177727802 1101 30-31 0.25643424331735076 1101 32-33 0.3173754184563151 1101 34-35 0.4015173710419546 1101 36-37 0.30939398808304475 1101 38-39 0.3715252671423386 1101 40-41 0.3608090855954842 1101 42-43 0.28002924609575786 1101 44-45 0.14974023409310178 1101 46-47 0.03001434082146659 1101 48-49 0.07821221706922188 1101 50-51 -0.06651275449564054 1101 52-53 0.08364901973420302 1101 54-55 -0.12368510565748636 1101 56-57 -0.2289231933790461 1101 58-59 -0.2679493476967849 1101 60-61 0.007028943148057465 1101 62-63 -0.1559769921806904 1101 64-65 -0.1706626560354465 1101 66-67 -0.1756773631959092 1101 68-69 -0.23884848507880463 1101 70-71 -0.35032485503442246 1101 72-73 -0.3306930762373632 1101 74-75 -0.4608103341355587 1101 76-77 -0.3135821594385355 1101 78-79 -0.38915068867343194 1101 80-81 -0.40697047134750264 1101 82-83 -0.3560565740731576 1101 84-85 -0.207947425965191 1101 86-87 -0.3148340505322089 1101 88-89 -0.16331022622344804 1101 90-91 -0.4523643798185262 1101 92-93 -0.5463376346489142 1101 94-95 -0.47310437423744034 1101 96-97 -0.5430063713697031 1101 98-99 -0.6762479854081072 1101 100-101 -0.5361639582484621 1102 1 0.2967788210254767 1102 2 0.2032062265538812 1102 3 0.15603863433369014 1102 4 0.05992131223871411 1102 5 0.0693751798751947 1102 6 0.10855440468411359 1102 7 0.08573607053913435 1102 8 0.09222147046894946 1102 9 0.24240737126244483 1102 10-11 0.217542851163941 1102 12-13 0.2388529647175801 1102 14-15 0.38359209618832324 1102 16-17 0.2177788050971543 1102 18-19 0.286233228220631 1102 20-21 0.17113729180422865 1102 22-23 0.13760479987806917 1102 24-25 0.15406715419869244 1102 26-27 0.12056821848663901 1102 28-29 0.16885742736444342 1102 30-31 0.3025236211355278 1102 32-33 0.356784302330027 1102 34-35 0.2685827140665751 1102 36-37 0.19146885639649724 1102 38-39 0.34459405480377825 1102 40-41 0.40063530046955975 1102 42-43 0.3496394630530517 1102 44-45 0.3189146176904174 1102 46-47 0.24516708807922782 1102 48-49 0.34787360104571974 1102 50-51 0.4143601457149657 1102 52-53 0.5273244106822119 1102 54-55 0.5840888548982264 1102 56-57 0.5159900282700818 1102 58-59 0.34260339316509913 1102 60-61 0.2462941482044556 1102 62-63 0.3477460937387846 1102 64-65 0.2249251022552059 1102 66-67 0.3044326002105393 1102 68-69 0.3501268496768546 1102 70-71 0.3454120642541696 1102 72-73 0.27699613894279196 1102 74-75 0.23896215536835186 1102 76-77 0.19444398142609387 1102 78-79 0.4740570265821056 1102 80-81 0.5054361602636668 1102 82-83 0.4304565763076269 1102 84-85 0.48692353720826276 1102 86-87 0.30264930876533924 1102 88-89 0.23979428698175553 1102 90-91 0.1611752357892442 1102 92-93 0.1155141545977898 1102 94-95 0.002278350497700643 1102 96-97 -0.04563096534569766 1102 98-99 0.07985276793487728 1102 100-101 0.05150456169982576 1103 1 0.3046411355855696 1103 2 0.16029429257808658 1103 3 0.24561771316780323 1103 4 0.1360997733617353 1103 5 0.10776271763826628 1103 6 0.1833501887215121 1103 7 0.133758888719413 1103 8 0.1831157169795503 1103 9 0.17737851099004587 1103 10-11 0.1916844268335609 1103 12-13 0.26854001641290637 1103 14-15 0.3015257597551866 1103 16-17 0.20720535486002234 1103 18-19 0.23553007553122285 1103 20-21 0.1976876875781386 1103 22-23 0.15592622061357986 1103 24-25 0.08508350835305833 1103 26-27 0.11052682678016623 1103 28-29 0.22676974108066617 1103 30-31 0.24609334991647813 1103 32-33 0.16684974426349442 1103 34-35 0.25135958703074124 1103 36-37 0.315016349265818 1103 38-39 0.23426680294304703 1103 40-41 0.1813409003750408 1103 42-43 0.3715317095974129 1103 44-45 0.30861837796450686 1103 46-47 0.24950034827736545 1103 48-49 0.1451405248107207 1103 50-51 0.14515899299181 1103 52-53 0.054179653631692304 1103 54-55 -0.09758170762393092 1103 56-57 0.0014514550814510585 1103 58-59 -0.006862227668300136 1103 60-61 0.1234375719441374 1103 62-63 -0.019370284043866093 1103 64-65 0.015395305289757744 1103 66-67 0.06614548269353548 1103 68-69 0.06560380365230856 1103 70-71 -0.05399976001883999 1103 72-73 -0.06912270293329215 1103 74-75 -0.010557262025447045 1103 76-77 0.026546393199737395 1103 78-79 0.04654483269508347 1103 80-81 0.09721984323947908 1103 82-83 0.032497681099105336 1103 84-85 -0.08652459024950332 1103 86-87 -0.0800824172814778 1103 88-89 -0.02256225552385871 1103 90-91 -0.012856426123637021 1103 92-93 0.06518441332760005 1103 94-95 -0.017178072178712256 1103 96-97 -0.022705191941032155 1103 98-99 0.07286090281867885 1103 100-101 -0.043193840014911444 1104 1 0.22442404626563217 1104 2 0.12974910197007716 1104 3 0.05329019659797041 1104 4 0.09027290403781052 1104 5 0.05853653983660223 1104 6 0.10565744294550683 1104 7 0.08143843608666401 1104 8 0.10355136586470337 1104 9 0.12765844907075063 1104 10-11 0.1603819331623697 1104 12-13 0.16070745369089678 1104 14-15 0.13277096455866655 1104 16-17 0.1353080371281976 1104 18-19 0.2377495966706462 1104 20-21 0.11619870934790555 1104 22-23 0.18529077891471957 1104 24-25 0.1877716912988845 1104 26-27 0.11458922432773022 1104 28-29 0.15296025951956693 1104 30-31 0.19520004916513045 1104 32-33 0.2559303424336363 1104 34-35 0.30277021977698837 1104 36-37 0.31703857024875504 1104 38-39 0.33841086670099685 1104 40-41 0.4428677500957008 1104 42-43 0.5027449745896959 1104 44-45 0.4875233578711544 1104 46-47 0.29471546037089524 1104 48-49 0.168779576215222 1104 50-51 0.29305066971360105 1104 52-53 0.3442100387007656 1104 54-55 0.23017124216127627 1104 56-57 0.38733127013473023 1104 58-59 0.3347139851150587 1104 60-61 0.46615216860685393 1104 62-63 0.5258395469962167 1104 64-65 0.4254779770127115 1104 66-67 0.3502636228163567 1104 68-69 0.5014356244919398 1104 70-71 0.35594940667317587 1104 72-73 0.500473342951878 1104 74-75 0.39653379854527415 1104 76-77 0.33175410336876254 1104 78-79 0.26873396031575325 1104 80-81 0.283291635147382 1104 82-83 0.17089993851708485 1104 84-85 0.107298098170034 1104 86-87 0.21943379597506407 1104 88-89 0.3186996289588002 1104 90-91 0.18189784012257704 1104 92-93 0.06325049155816842 1104 94-95 -0.09313504131031891 1104 96-97 -0.13922339763341185 1104 98-99 0.2096701832772574 1104 100-101 -0.022647162262263265 1105 1 0.24397482094248346 1105 2 0.20618139201374674 1105 3 0.16749322882735385 1105 4 0.08332428740417441 1105 5 0.0924793936555588 1105 6 0.13926666012509514 1105 7 0.15184740064971436 1105 8 0.0881114266921017 1105 9 0.10191482992341605 1105 10-11 0.11514446115585031 1105 12-13 0.08579480998407973 1105 14-15 0.17525063682194997 1105 16-17 0.12111289916549595 1105 18-19 0.21995591956277138 1105 20-21 3.20821525718884E-5 1105 22-23 0.007957919237391309 1105 24-25 0.10074965798508373 1105 26-27 0.08722520307138382 1105 28-29 0.1944824403315195 1105 30-31 0.11636000778255351 1105 32-33 0.16421080495456408 1105 34-35 0.28456022397342196 1105 36-37 0.3018373287714411 1105 38-39 0.26521792666833477 1105 40-41 0.27436712063180124 1105 42-43 0.3022266536586713 1105 44-45 0.3952834220224588 1105 46-47 0.3389511914838579 1105 48-49 0.2947812132368455 1105 50-51 0.2585830549039514 1105 52-53 0.3176115535689945 1105 54-55 0.3447415655353723 1105 56-57 0.3845204957071644 1105 58-59 0.45798752976544677 1105 60-61 0.3165227836533262 1105 62-63 0.24006958592742222 1105 64-65 0.42885360699597186 1105 66-67 0.3880110976897342 1105 68-69 0.42513566728717933 1105 70-71 0.2596219026325599 1105 72-73 0.12280206852639708 1105 74-75 0.1427817366874642 1105 76-77 0.1828597161923753 1105 78-79 0.22011088662069866 1105 80-81 0.1336732606184441 1105 82-83 0.12775320071892082 1105 84-85 0.367911731984087 1105 86-87 0.2548557860781493 1105 88-89 0.19674775074656026 1105 90-91 0.23366844104246454 1105 92-93 0.36478384898979144 1105 94-95 0.2573438082872812 1105 96-97 0.274178574271307 1105 98-99 0.3066510417579842 1105 100-101 0.2378455646251929 1106 1 0.12978802139811307 1106 2 0.15243247456032094 1106 3 0.11599814134905984 1106 4 0.07740878363072312 1106 5 0.04932940709407063 1106 6 -0.004146123950057756 1106 7 0.011152181772665415 1106 8 0.025740105750642783 1106 9 0.07220505906066421 1106 10-11 -0.035760185155382374 1106 12-13 -0.1337112928055859 1106 14-15 0.005035705998274409 1106 16-17 0.12067134814822111 1106 18-19 0.049573114028660825 1106 20-21 0.19649711429833872 1106 22-23 0.21439965796717786 1106 24-25 0.1547705452679864 1106 26-27 0.10082156144869003 1106 28-29 0.07690998970203822 1106 30-31 0.1501004781730444 1106 32-33 0.10575179667387857 1106 34-35 -0.31808950874956565 1106 36-37 -0.0074823840284139465 1106 38-39 0.09606399814414601 1106 40-41 -0.021943901932097276 1106 42-43 -0.2351390145765393 1106 44-45 0.009149830442211737 1106 46-47 -0.07927313349768639 1106 48-49 -0.18800981581166099 1106 50-51 -0.01786755750811153 1106 52-53 0.1545843053917224 1106 54-55 -0.027210345932587643 1106 56-57 -0.07671687102761382 1106 58-59 0.030491060040489515 1106 60-61 0.005094718073394233 1106 62-63 -0.09239955882878803 1106 64-65 -0.22256404907204796 1106 66-67 -0.20431615347386867 1106 68-69 0.15761739731392765 1106 70-71 0.05320085494077631 1106 72-73 0.05121022378170892 1106 74-75 0.09676785449412861 1106 76-77 -0.054434279993650136 1106 78-79 -0.05806988052620099 1106 80-81 -0.21391996637290944 1106 82-83 -0.4271501486695186 1106 84-85 -0.5440756315510491 1106 86-87 -0.12866817499710592 1106 88-89 -0.04841758557879672 1106 90-91 -0.17598296829452664 1106 92-93 -0.21470962586740328 1106 94-95 -0.08321617225887579 1106 96-97 -0.14209524708611454 1106 98-99 -0.5961605930046652 1106 100-101 -1.0504153642957554 1107 1 0.2605353304966229 1107 2 0.2023597359627871 1107 3 0.23055055366811672 1107 4 0.11326059313665837 1107 5 0.10012270575747095 1107 6 0.027801691972229037 1107 7 0.10598752803824851 1107 8 0.12123244580038772 1107 9 0.18025877896800324 1107 10-11 0.2090935057418477 1107 12-13 0.13643175265924157 1107 14-15 0.1520022291786418 1107 16-17 0.17971162528014162 1107 18-19 0.18205783039079648 1107 20-21 0.22232507578314653 1107 22-23 0.1713337154157344 1107 24-25 0.14183246053284648 1107 26-27 0.2056965406509974 1107 28-29 0.22505568873915394 1107 30-31 0.2927931288016623 1107 32-33 0.19828723807558646 1107 34-35 0.26672582906896025 1107 36-37 0.29387554533195726 1107 38-39 0.15152365915241006 1107 40-41 -0.009072369814056458 1107 42-43 0.24999735429560843 1107 44-45 0.09560189336004044 1107 46-47 -0.04321441361167899 1107 48-49 -0.017320697591181045 1107 50-51 -0.040143059745730625 1107 52-53 -0.08684704515712838 1107 54-55 0.0450600368729539 1107 56-57 0.16700457214029285 1107 58-59 0.13091746607118182 1107 60-61 0.11620431231574457 1107 62-63 -0.07808328031462253 1107 64-65 -0.012229195551803684 1107 66-67 -0.02759399785166039 1107 68-69 0.12577260996233264 1107 70-71 0.1214053421230048 1107 72-73 0.10114601757098285 1107 74-75 -0.04830106586030425 1107 76-77 0.001649525109574057 1107 78-79 -0.022882743952553852 1107 80-81 0.01042485297513096 1107 82-83 6.831370246516144E-4 1107 84-85 0.06568243262102769 1107 86-87 -0.07189580627854042 1107 88-89 -0.006755433966816327 1107 90-91 0.17124805887685923 1107 92-93 -0.029483666933778352 1107 94-95 -0.04074536368724324 1107 96-97 9.301666279917242E-4 1107 98-99 -0.10131074168150533 1107 100-101 -0.028714944928942998 1108 1 0.15340788932170568 1108 2 0.04655375850076382 1108 3 0.1062953068882635 1108 4 0.056074640698824396 1108 5 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-0.17224139961329854 2316 58-59 -0.3247289829468585 2316 60-61 -0.2601480844510391 2316 62-63 -0.1968780459841497 2316 64-65 -0.24037646774478105 2316 66-67 -0.11062154677303937 2316 68-69 0.07706490610704009 2316 70-71 0.10344738246388374 2316 72-73 -0.07761925542214954 2316 74-75 -0.07096971050718892 2316 76-77 0.003211610202846771 2316 78-79 -0.1069021744217089 2316 80-81 -0.13059634422218025 2316 82-83 -0.06184859504163853 2316 84-85 -0.1362085683951051 2316 86-87 -0.10248781953431418 2316 88-89 -0.03367396266109779 2316 90-91 0.08594270523028413 2316 92-93 0.2924823580694955 2316 94-95 0.24323016411836917 2316 96-97 0.1883825021434795 2316 98-99 0.3965104116131428 2316 100-101 0.5140094277763474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 11.0 5 37.0 6 60.0 7 166.0 8 354.0 9 720.0 10 1133.0 11 1913.0 12 3163.0 13 4700.0 14 6006.0 15 8707.0 16 12295.0 17 17673.0 18 15453.0 19 8709.0 20 7398.0 21 7454.0 22 7587.0 23 8163.0 24 9399.0 25 11391.0 26 13940.0 27 17254.0 28 21992.0 29 27379.0 30 34659.0 31 44237.0 32 57334.0 33 76698.0 34 107544.0 35 158817.0 36 248129.0 37 368825.0 38 273774.0 39 19670.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43986136566304 19.23083403380616 15.089963176034281 22.239341424496516 2 15.821576442460076 20.154821593912455 39.778492938378335 24.245109025249135 3 19.34957959704208 24.528122948835378 29.852603154082864 26.26969430003968 4 12.945391290458637 16.710549631008742 36.33353465423136 34.010524424301266 5 13.699109807842829 37.878294216559745 33.28333726560832 15.1392587099891 6 32.472728089506276 35.83489107457941 16.832527633788967 14.85985320212535 7 29.60970782680746 33.17876547030475 19.417712578646178 17.793814124241614 8 27.618689900096584 34.43560684524329 18.811441349482262 19.13426190517786 9 27.30635134157085 16.493609725296803 19.84859753373581 36.35144139939654 10-11 25.68738192154974 26.782875411480784 26.10987503961945 21.419867627350026 12-13 26.340998798267407 23.974128431894957 27.52304568705286 22.161827082784775 14-15 23.030788370972854 25.22099544033123 26.144066316102094 25.60414987259382 16-17 22.12755841841637 28.958576145470154 26.667542246192166 22.24632318992131 18-19 22.04488790502312 26.59962763952911 29.85172965431871 21.50375480112906 20-21 22.839148762001262 25.648729556985877 30.06458282899121 21.44753885202165 22-23 22.504656103473124 25.612780653071155 30.01403850293405 21.86852474052167 24-25 22.398697235056105 25.875388786113824 30.2263304518186 21.49958352701147 26-27 22.118866966843022 26.328916791376184 30.026027554954084 21.526188686826714 28-29 21.71472781640105 26.247705091149022 30.342555604257104 21.69501148819282 30-31 21.775818781243213 25.86423442737099 30.390990973003866 21.96895581838193 32-33 21.950549930494375 25.994198713709203 30.12522866975969 21.930022686036732 34-35 22.41306805561448 26.05219285876507 29.74107595205235 21.793663133568096 36-37 22.553514339122557 25.7309321240769 29.780601816380365 21.93495172042018 38-39 22.001368898916112 26.07661965574126 29.923918170542095 21.99809327480053 40-41 22.0014194464159 26.02135408948752 29.97892668013321 21.998299783963375 42-43 22.272547589920325 25.860219029228958 29.539430845987212 22.3278025348635 44-45 22.08803255408835 26.22274368771635 29.367280445834282 22.32194331236102 46-47 22.392821578938175 26.66080381944011 28.75782718181523 22.188547419806483 48-49 22.691901658900836 26.5599145966802 28.553490629843242 22.194693114575717 50-51 22.694834122394784 26.742132886766978 28.211671454277276 22.351361536560958 52-53 22.915462354488152 26.96975038176631 27.857008043999443 22.257779219746094 54-55 22.453190478463362 26.85760153436712 28.041507525680153 22.64770046148936 56-57 22.55285921429944 27.248388392935137 28.032915966826977 22.16583642593845 58-59 22.87789471582557 26.58284396548927 28.1160232301023 22.42323808858286 60-61 22.577972332518897 26.69081477562287 28.720734638258786 22.010478253599445 62-63 22.42401799908657 26.87172281606341 28.506259249738577 22.197999935111447 64-65 22.37386811517975 26.65345809413463 28.356346946835654 22.616326843849965 66-67 22.409698842238456 27.04636037605413 28.043429160416984 22.500511621290435 68-69 22.287471268097043 26.74980720612348 28.395231190430437 22.56749033534904 70-71 22.42982053323417 26.939918190507804 28.25678147781186 22.37347979844617 72-73 22.571920227010107 26.84377082361045 28.080272132612237 22.504036816767204 74-75 22.36820760344109 27.266326334520464 27.73570767207321 22.629758389965236 76-77 22.42613935565666 27.187586570065914 27.929562226875344 22.456711847402087 78-79 22.527652506819535 26.913182858440628 27.957139862286525 22.602024772453312 80-81 22.194069186172747 27.632946664104402 27.8623027450354 22.31068140468745 82-83 22.42915315721153 27.41147868753141 27.619591629436048 22.539776525821008 84-85 22.32890011405411 27.110562608719523 27.65933883554994 22.90119844167642 86-87 22.41063473484291 26.844082787811935 27.90915976809829 22.83612270924687 88-89 22.393632685862034 27.049667196589855 27.74924691841762 22.807453199130492 90-91 22.307669747395234 27.073064498113308 27.96548984957034 22.653775904921115 92-93 22.381018043054254 26.629696229289923 28.098197940753295 22.89108778690253 94-95 22.184273510246154 26.6201860804069 28.09665025319015 23.098890156156802 96-97 22.284195643981462 26.669757192022704 28.03856251887383 23.007484645122002 98-99 22.358348149259683 26.773769773763224 27.622013404058777 23.245868672918316 100-101 22.150561187031244 26.57637376232604 28.214301649691116 23.058763400951605 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 460.0 1 323.0 2 316.0 3 441.0 4 332.5 5 283.0 6 625.0 7 1434.5 8 2324.5 9 2755.0 10 2491.0 11 2069.5 12 2124.5 13 2411.0 14 2634.5 15 2833.5 16 3059.5 17 3087.0 18 3076.0 19 3208.5 20 3380.5 21 3718.5 22 4360.5 23 5058.5 24 5846.0 25 7134.5 26 8873.0 27 10761.5 28 12092.0 29 13864.5 30 16606.0 31 18975.5 32 21734.0 33 24716.0 34 28538.5 35 32076.0 36 35695.0 37 39630.5 38 41691.0 39 42689.0 40 43829.0 41 48176.5 42 53198.5 43 56688.0 44 59161.5 45 61481.0 46 61536.5 47 61820.0 48 65239.0 49 64173.0 50 60828.5 51 58021.0 52 55686.5 53 54886.5 54 54153.5 55 50295.0 56 43597.5 57 38470.5 58 35332.0 59 31814.0 60 27419.5 61 22890.5 62 18713.0 63 14747.5 64 10943.5 65 8490.5 66 6775.5 67 5814.0 68 4305.5 69 2523.0 70 1800.5 71 1384.0 72 1231.5 73 1050.5 74 809.0 75 703.0 76 482.5 77 266.0 78 159.5 79 116.5 80 83.0 81 57.5 82 31.5 83 20.0 84 18.5 85 7.5 86 3.5 87 5.0 88 3.5 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016159745636868676 2 0.0 3 0.0 4 0.0 5 0.038808346664603546 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038683560984009964 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.111069238582734E-4 24-25 1.871785208903708E-4 26-27 0.0014662317469745713 28-29 9.670890246002491E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.551248231162978E-4 42-43 0.011012336312383481 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.119642014839513E-5 54-55 1.5598210074197567E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.239284029679026E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.799105037098783E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012790532260842003 92-93 0.0016222138477165469 94-95 0.0 96-97 0.0 98-99 0.0015286245872713612 100-101 0.018499477147998312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1602748.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.45862575975223 #Duplication Level Percentage of deduplicated Percentage of total 1 72.03257177420674 32.74501722796156 2 14.501356806013105 13.184235041063713 3 5.296523123070207 7.223179874385679 4 2.530976034590133 4.602187694533383 5 1.409407910600914 3.2034873375420645 6 0.8774281047099757 2.3932005505859686 7 0.5844335617186124 1.8597282594523818 8 0.44116777669361623 1.6043904686381634 9 0.31300214098969054 1.280578247032639 >10 1.749004231851687 14.83110344663649 >50 0.16183187932545093 5.16253222679217 >100 0.09540343764039648 8.070304195976282 >500 0.005100981756174895 1.483240445997425 >1k 0.0013786437178851068 0.9767314129746005 >5k 4.13593115365532E-4 1.38008357042757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9135 0.569958596111179 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7692 0.4799257275629107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5194 0.3240684125015286 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2585 0.16128549216720284 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2214 0.13813774841709364 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1858 0.1159258972714363 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1722 0.10744047099107282 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 9.35892604451854E-5 0.0 26-27 0.0 0.0 0.0 3.1196420148395135E-4 0.0 28-29 0.0 0.0 0.0 5.615355626711123E-4 0.0 30-31 0.0 0.0 0.0 0.0011230711253422247 0.0 32-33 0.0 0.0 0.0 0.0019653744693488933 0.0 34-35 0.0 0.0 0.0 0.002838874233503957 0.0 36-37 0.0 0.0 0.0 0.004055534619291367 0.0 38-39 0.0 0.0 0.0 0.005552962786414334 0.0 40-41 0.0 0.0 0.0 0.008048676398285944 0.0 42-43 0.0 0.0 0.0 0.01172985397579657 0.0 44-45 0.0 0.0 0.0 0.018811441349482264 0.0 46-47 0.0 0.0 0.0 0.034097687222195874 0.0 48-49 0.0 0.0 0.0 0.046732237382295906 0.0 50-51 0.0 0.0 0.0 0.06173771547367396 0.0 52-53 0.0 0.0 0.0 0.08307606685517624 0.0 54-55 0.0 0.0 0.0 0.10793961371344715 0.0 56-57 0.0 0.0 0.0 0.13342708897468597 0.0 58-59 0.0 0.0 0.0 0.16696324063421075 0.0 60-61 0.0 0.0 0.0 0.20979592549795725 0.0 62-63 0.0 0.0 0.0 0.26161317936444156 0.0 64-65 0.0 0.0 0.0 0.3066920064788725 0.0 66-67 0.0 0.0 0.0 0.37329636349569617 0.0 68-69 0.0 0.0 0.0 0.45799464419858893 0.0 70-71 0.0 0.0 0.0 0.5253789117191223 0.0 72-73 0.0 0.0 0.0 0.6263617237394774 3.119642014839513E-5 74-75 0.0 0.0 0.0 0.7157394674646295 6.239284029679026E-5 76-77 0.0 0.0 0.0 0.802933461779394 6.239284029679026E-5 78-79 0.0 0.0 0.0 0.9131816105838222 6.239284029679026E-5 80-81 0.0 0.0 0.0 1.0346604706416729 6.239284029679026E-5 82-83 0.0 0.0 0.0 1.1728606118990634 6.239284029679026E-5 84-85 0.0 0.0 0.0 1.3563579552119236 6.239284029679026E-5 86-87 0.0 0.0 0.0 1.5862443752854474 6.239284029679026E-5 88-89 0.0 0.0 0.0 1.8548143563429809 6.239284029679026E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5965 0.0 36.25374 1 GTATCAA 9235 0.0 34.944973 1 GTACATG 8820 0.0 27.266773 1 ATCAACG 11780 0.0 27.147305 3 TATCAAC 11960 0.0 26.937387 2 TCAACGC 11890 0.0 26.816225 4 CAACGCA 12070 0.0 26.495049 5 AACGCAG 12355 0.0 25.911013 6 TACATGG 9090 0.0 25.823792 2 ACATGGG 9025 0.0 24.851448 3 CATGGGG 3290 0.0 23.97558 4 ACGCAGA 14035 0.0 22.743889 7 CGCAGAG 14035 0.0 22.743889 8 AGTACTT 7790 0.0 22.134872 12-13 GAGTACT 7625 0.0 21.772846 12-13 GCAGAGT 14805 0.0 21.208057 9 AGAGTAC 12840 0.0 20.6617 10-11 GTACTTT 7955 0.0 20.658707 14-15 GGACCGA 350 3.6379788E-12 20.355907 6 CATGGGA 5575 0.0 20.200422 4 >>END_MODULE