FastQCFastQC Report
Thu 26 May 2016
SRR937606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618917
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76430.47210573488325835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.35431093749710457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38440.23744268544959377No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22510.13904357048570123No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG21410.1322489046689855No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21250.13126058964109957No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17960.11093836188019522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51500.040.7179681
GTATCAA85000.038.096311
TATCAAC106400.030.4246562
ATCAACG105850.030.356323
TCAACGC107300.029.9922544
CAACGCA109850.029.3807585
AACGCAG113100.028.6019336
GTACATG97950.026.7486521
TACATGG100850.025.782892
ACGCAGA129350.024.9000977
CGCAGAG129700.024.759658
GAGTACT62550.024.45265212-13
ACATGGG100200.024.4305233
GCAGAGT134900.023.2770169
GTACTTT66650.022.5903514-15
GTCCTAC2955.456968E-1222.5663221
AGTACTT65000.022.28865812-13
CATGGGA59150.021.3770144
CATGGGG39900.020.729874
GTACCTG11750.020.6389241
Produced by FastQC (version 0.11.5)