##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937606_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1618917 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65726964384215 33.0 31.0 34.0 30.0 34.0 2 31.76804616913653 33.0 31.0 34.0 30.0 34.0 3 31.817348264302616 33.0 31.0 34.0 30.0 34.0 4 35.410996981315286 37.0 35.0 37.0 33.0 37.0 5 35.39179834420171 37.0 35.0 37.0 33.0 37.0 6 35.493281002052605 37.0 35.0 37.0 33.0 37.0 7 35.42226994960211 37.0 35.0 37.0 33.0 37.0 8 35.40910065185553 37.0 35.0 37.0 33.0 37.0 9 36.99935327135363 39.0 37.0 39.0 33.0 39.0 10-11 36.88535298597766 39.0 37.0 39.0 33.0 39.0 12-13 36.75826988042006 39.0 37.0 39.0 33.0 39.0 14-15 37.87681888571187 40.0 38.0 41.0 33.0 41.0 16-17 37.82342176899742 40.0 38.0 41.0 33.0 41.0 18-19 37.77303901311803 40.0 38.0 41.0 33.0 41.0 20-21 37.601401739557986 40.0 37.0 41.0 32.0 41.0 22-23 37.45499614865987 40.0 37.0 41.0 32.0 41.0 24-25 37.300889730603856 39.5 37.0 41.0 32.0 41.0 26-27 37.401013764139854 40.0 37.0 41.0 32.0 41.0 28-29 37.33795401493715 40.0 37.0 41.0 32.0 41.0 30-31 37.12311224108463 39.0 36.5 41.0 31.0 41.0 32-33 36.88992363413319 39.0 36.0 41.0 31.0 41.0 34-35 36.61785100780337 39.0 35.5 40.5 30.0 41.0 36-37 36.64018723628203 39.0 36.0 41.0 30.0 41.0 38-39 36.58513530959277 39.0 35.5 41.0 30.0 41.0 40-41 36.37866240208732 39.0 35.0 41.0 30.0 41.0 42-43 36.114638057417395 39.0 35.0 41.0 29.5 41.0 44-45 35.85450767395734 39.0 35.0 40.5 28.5 41.0 46-47 35.45696814598895 39.0 35.0 40.0 27.0 41.0 48-49 35.106765510523395 38.5 35.0 40.0 26.0 41.0 50-51 34.196565975896235 37.5 33.5 39.5 24.0 40.5 52-53 34.28957630317058 38.0 33.5 39.5 24.0 40.5 54-55 34.57735201989972 38.0 34.0 40.0 24.0 41.0 56-57 34.29570787137327 38.0 34.0 40.0 23.0 41.0 58-59 34.06581436849449 38.0 34.0 40.0 23.0 41.0 60-61 33.75970479030117 37.5 33.0 40.0 22.0 41.0 62-63 33.377828820130986 37.0 33.0 40.0 21.0 41.0 64-65 33.002016471505335 36.0 32.0 39.0 20.0 41.0 66-67 32.58061006215884 36.0 32.0 39.0 18.5 40.5 68-69 32.1001153857795 35.0 31.0 39.0 15.5 40.0 70-71 31.650107757222884 35.0 31.0 38.0 12.0 40.0 72-73 31.17858080432783 35.0 31.0 37.5 8.0 39.5 74-75 30.68243337984591 35.0 30.0 37.0 7.0 39.0 76-77 30.21357487752615 34.0 30.0 36.0 3.5 39.0 78-79 29.680105280258346 34.0 29.0 36.0 2.0 38.0 80-81 29.15628719693474 34.0 29.0 35.0 2.0 37.0 82-83 28.65179561398145 34.0 28.5 35.0 2.0 37.0 84-85 28.179153409347112 34.0 27.0 35.0 2.0 36.0 86-87 27.634715986057344 33.0 26.0 35.0 2.0 36.0 88-89 27.1471733881354 33.0 25.5 35.0 2.0 35.0 90-91 26.63373971611886 32.5 25.0 35.0 2.0 35.0 92-93 26.062992729089878 32.0 24.0 34.5 2.0 35.0 94-95 25.408108321797844 32.0 21.5 34.0 2.0 35.0 96-97 24.599346661996876 31.5 15.5 34.0 2.0 35.0 98-99 23.63148666670373 31.0 2.0 34.0 2.0 35.0 100-101 21.100736171156395 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23748770630551874 1101 2 0.36177808818336743 1101 3 0.3337417706694268 1101 4 0.35815538412926173 1101 5 0.3109368975904161 1101 6 0.3315917319010069 1101 7 0.2745979001441228 1101 8 0.2856149997034265 1101 9 0.5305401433581736 1101 10-11 0.21006060435049534 1101 12-13 0.2687665014213536 1101 14-15 0.34938688268611173 1101 16-17 0.45410791297108233 1101 18-19 0.4866672528185134 1101 20-21 0.48929059768830996 1101 22-23 0.4858924903586157 1101 24-25 0.412262476060576 1101 26-27 0.46635964044665457 1101 28-29 0.45732555103588624 1101 30-31 0.3869121549975034 1101 32-33 0.38240730938847634 1101 34-35 0.5200226868224291 1101 36-37 0.5724052722745014 1101 38-39 0.6601563139531663 1101 40-41 0.5625856885130958 1101 42-43 0.4492524662466053 1101 44-45 0.7690702636031048 1101 46-47 0.9184925258354539 1101 48-49 0.9109774333521443 1101 50-51 0.7774928011364182 1101 52-53 0.6535951352909564 1101 54-55 0.8048754860972522 1101 56-57 0.8183364107098043 1101 58-59 0.8918400878762114 1101 60-61 1.012429155302506 1101 62-63 0.8964622975750984 1101 64-65 0.9347207545205691 1101 66-67 1.076104876299091 1101 68-69 1.3369180550117647 1101 70-71 1.2209953023007536 1101 72-73 1.0987000941147116 1101 74-75 1.0610866228677338 1101 76-77 0.9740385777454925 1101 78-79 0.8443108805236612 1101 80-81 0.9841267329176269 1101 82-83 1.1035389594589518 1101 84-85 1.3055910283451908 1101 86-87 1.3972525742635575 1101 88-89 1.2731085863256837 1101 90-91 1.2774221762338662 1101 92-93 1.4014519778663406 1101 94-95 1.5390909539751476 1101 96-97 1.3504510745772222 1101 98-99 1.845726505168031 1101 100-101 2.2476142551975613 1102 1 0.3209636088547718 1102 2 0.49640017068137965 1102 3 0.41598258366224883 1102 4 0.3449261091004203 1102 5 0.35008391649681414 1102 6 0.32887851533281776 1102 7 0.32558715827133966 1102 8 0.35658576401986153 1102 9 0.33002467431118987 1102 10-11 0.4173891596057331 1102 12-13 0.4812673530092866 1102 14-15 0.5694814108604689 1102 16-17 0.6250824663754031 1102 18-19 0.55293916541995 1102 20-21 0.5904447002753841 1102 22-23 0.7427220342213232 1102 24-25 0.7602258987321804 1102 26-27 0.7831484382188236 1102 28-29 0.6304399822555169 1102 30-31 0.609966381820719 1102 32-33 0.4976120387825418 1102 34-35 0.7343761727450087 1102 36-37 0.8723605686921658 1102 38-39 0.921777331679209 1102 40-41 0.847506726217965 1102 42-43 0.8757196661412792 1102 44-45 0.6796929523551825 1102 46-47 0.7978253861069078 1102 48-49 0.7972623130543468 1102 50-51 0.5932304140722877 1102 52-53 0.6742950372339607 1102 54-55 0.7365787078936208 1102 56-57 0.8078821641373963 1102 58-59 0.7967146537577392 1102 60-61 0.7900875831908039 1102 62-63 0.9567172151605163 1102 64-65 0.9394396998834509 1102 66-67 0.729571929988559 1102 68-69 0.7368271934497272 1102 70-71 0.8843767532228846 1102 72-73 0.9729085000722364 1102 74-75 0.8417577583616946 1102 76-77 1.04955911804646 1102 78-79 1.1899922389001851 1102 80-81 1.2049777390926941 1102 82-83 1.1808309940307424 1102 84-85 1.3964786164414846 1102 86-87 1.4730863841033965 1102 88-89 1.481076502319688 1102 90-91 1.7141578043000614 1102 92-93 1.7780429163583484 1102 94-95 1.7882998383528488 1102 96-97 1.9860097124345728 1102 98-99 2.2469625898384535 1102 100-101 2.275858269395542 1103 1 0.15182902301881285 1103 2 0.3401698212572235 1103 3 0.19811905595348378 1103 4 0.07477232897452524 1103 5 0.18442876770012617 1103 6 0.18200679354422533 1103 7 0.20146804615398395 1103 8 0.21408548923891857 1103 9 0.19385445518179978 1103 10-11 0.400067492436861 1103 12-13 0.2557566903871944 1103 14-15 0.43155128522388964 1103 16-17 0.24646591925402817 1103 18-19 0.3477189256913391 1103 20-21 0.4954318061329559 1103 22-23 0.4271798633845876 1103 24-25 0.396672020358082 1103 26-27 0.27214635885610505 1103 28-29 0.41031483916381006 1103 30-31 0.415019023867508 1103 32-33 0.40072988653370345 1103 34-35 0.3785380310567419 1103 36-37 0.44353406650027694 1103 38-39 0.440120922254863 1103 40-41 0.6272041905319554 1103 42-43 0.6338619675269968 1103 44-45 0.29851234388922876 1103 46-47 0.5432858927954882 1103 48-49 0.548575840725718 1103 50-51 0.34321022608474294 1103 52-53 0.37752040471140447 1103 54-55 0.43335644338462487 1103 56-57 0.8803815267203845 1103 58-59 0.8455353762035642 1103 60-61 0.7371410342525024 1103 62-63 0.672661762497242 1103 64-65 0.878764890230002 1103 66-67 0.9033948020812019 1103 68-69 0.8043350761111583 1103 70-71 0.7685506046946209 1103 72-73 0.7261518498126662 1103 74-75 0.751922335322412 1103 76-77 0.8746707688331519 1103 78-79 0.8863310725878044 1103 80-81 0.9865541816552508 1103 82-83 0.6964740674200556 1103 84-85 0.8716507564205038 1103 86-87 1.0651847310062976 1103 88-89 0.8810175459190148 1103 90-91 1.0758214888769615 1103 92-93 1.3137965900113038 1103 94-95 1.0469429139852764 1103 96-97 1.2189144124223823 1103 98-99 1.3642106830082597 1103 100-101 1.5226160412099716 1104 1 0.37284378068598656 1104 2 0.395354816882044 1104 3 0.5087341041983464 1104 4 0.3222618116503213 1104 5 0.4395730384216776 1104 6 0.36026433374433964 1104 7 0.38814376978755405 1104 8 0.45672218300573064 1104 9 0.5168225930552524 1104 10-11 0.4269227358467802 1104 12-13 0.3610134656195356 1104 14-15 0.5472440134700705 1104 16-17 0.5549672821231297 1104 18-19 0.5846085336866267 1104 20-21 0.6639943821481964 1104 22-23 0.7262918953921655 1104 24-25 0.5370418386448179 1104 26-27 0.5756907619729006 1104 28-29 0.7021020884953444 1104 30-31 0.5856862440154913 1104 32-33 0.7609510903712859 1104 34-35 0.6596363691190348 1104 36-37 0.7682461815300456 1104 38-39 0.8691654436940013 1104 40-41 0.9494629839269706 1104 42-43 0.8991397027192107 1104 44-45 0.9694639700943668 1104 46-47 0.8809425126756878 1104 48-49 0.8740491891068274 1104 50-51 0.9264873464724275 1104 52-53 1.0003580735173117 1104 54-55 0.9565460524019684 1104 56-57 1.0096160715016609 1104 58-59 1.0732994217745713 1104 60-61 1.0269773405823983 1104 62-63 1.1675499223212569 1104 64-65 1.1063150907490495 1104 66-67 1.0415960978878758 1104 68-69 1.0955075209183036 1104 70-71 1.1622816033431498 1104 72-73 1.0708816699548294 1104 74-75 1.030377878744357 1104 76-77 1.1400520852224112 1104 78-79 1.1012891801848887 1104 80-81 1.3319080441135718 1104 82-83 1.441638181704512 1104 84-85 1.4201715697549986 1104 86-87 1.3596802257155964 1104 88-89 1.450128610906681 1104 90-91 1.5893237991110958 1104 92-93 1.5495356015597217 1104 94-95 1.6628392667478522 1104 96-97 1.7757567071999283 1104 98-99 2.050950088440395 1104 100-101 2.054944971064863 1105 1 0.27010788383244133 1105 2 0.2570487668112911 1105 3 0.33365242911666826 1105 4 0.2253465382644606 1105 5 0.2939409810249103 1105 6 0.22961623250800756 1105 7 0.26954747472067453 1105 8 0.40242645223941054 1105 9 0.4026871259786091 1105 10-11 0.43375083091016364 1105 12-13 0.45551925130178716 1105 14-15 0.59110833674616 1105 16-17 0.4716004634621953 1105 18-19 0.4394423591086891 1105 20-21 0.6916387039101579 1105 22-23 0.6064799373449077 1105 24-25 0.6671381599176129 1105 26-27 0.7035115368524956 1105 28-29 0.6323798128907256 1105 30-31 0.74942076069118 1105 32-33 0.6716821187493665 1105 34-35 0.7315992499054502 1105 36-37 0.7528909749983725 1105 38-39 0.8016575673626036 1105 40-41 0.8671720634007656 1105 42-43 0.8037807691249839 1105 44-45 0.7610032469277641 1105 46-47 0.7465993783325544 1105 48-49 0.9267987712681744 1105 50-51 0.8535461117664838 1105 52-53 0.8923183919482227 1105 54-55 0.7516857298945823 1105 56-57 0.9408736057003679 1105 58-59 0.917809575108258 1105 60-61 0.8185501027433943 1105 62-63 0.9744706115948816 1105 64-65 1.0466893693586172 1105 66-67 1.079996489229444 1105 68-69 1.0307498091017706 1105 70-71 1.2965388405415688 1105 72-73 1.264297289841032 1105 74-75 1.370366056967832 1105 76-77 1.2269829074473542 1105 78-79 1.1904489605211381 1105 80-81 1.3460388523620281 1105 82-83 1.4932914833578153 1105 84-85 1.2162284540471795 1105 86-87 1.321383202124455 1105 88-89 1.278166894827315 1105 90-91 1.310951535444719 1105 92-93 1.3321255606202698 1105 94-95 1.4889703428789254 1105 96-97 1.6352115078311975 1105 98-99 2.054578150891988 1105 100-101 2.1259993803545676 1106 1 0.2620895211954988 1106 2 0.3782680606507647 1106 3 0.2121537074883264 1106 4 0.19003264701674993 1106 5 0.2788267444932657 1106 6 0.2995071132333962 1106 7 0.30952575727453535 1106 8 0.3072961622252066 1106 9 0.4010700476202729 1106 10-11 0.19830443140215692 1106 12-13 0.10832435858824141 1106 14-15 0.25008997117669196 1106 16-17 0.19688440524569728 1106 18-19 0.24165021567970513 1106 20-21 0.40785360105601853 1106 22-23 0.2634328925137197 1106 24-25 0.2318246457088975 1106 26-27 0.17361004038472316 1106 28-29 0.5966059340685419 1106 30-31 0.48849804572553523 1106 32-33 0.5946618495519047 1106 34-35 0.3695328918801053 1106 36-37 0.444081794015041 1106 38-39 0.42672663881191397 1106 40-41 0.4727622530136699 1106 42-43 0.3495403162760198 1106 44-45 0.18019666835137116 1106 46-47 0.3782930249322263 1106 48-49 0.56288722629224 1106 50-51 0.2606007627310163 1106 52-53 0.28838591711871686 1106 54-55 0.3620077589006101 1106 56-57 0.4411695813118115 1106 58-59 0.44822112155045346 1106 60-61 0.21313544572948473 1106 62-63 0.3989052939877098 1106 64-65 0.4094212012109608 1106 66-67 0.6180204529901303 1106 68-69 0.6188866541483833 1106 70-71 0.3382056293967963 1106 72-73 0.3075591631131971 1106 74-75 0.43789374105607237 1106 76-77 0.6360989143865474 1106 78-79 0.5841363222996883 1106 80-81 0.40856427631095826 1106 82-83 0.28262366769972047 1106 84-85 0.2191253703290208 1106 86-87 0.25087544370926196 1106 88-89 0.6064777894528817 1106 90-91 0.9335455553548897 1106 92-93 0.7375120234959631 1106 94-95 0.6837884864981056 1106 96-97 0.49970285499571787 1106 98-99 0.8576567580764198 1106 100-101 0.8203030752110401 1107 1 0.4612575693760199 1107 2 0.5712918035925121 1107 3 0.5305135790164925 1107 4 0.4043099213227421 1107 5 0.4198378336036015 1107 6 0.5116123076061285 1107 7 0.48604652653009595 1107 8 0.5740739467663332 1107 9 0.6418065944204727 1107 10-11 0.6774342477533182 1107 12-13 0.669098286825907 1107 14-15 0.8343848441191923 1107 16-17 0.7868972685420701 1107 18-19 0.8137285376685099 1107 20-21 0.8578747265810307 1107 22-23 0.8581481992097935 1107 24-25 0.8880141112331188 1107 26-27 0.9503756777489727 1107 28-29 0.8548055022484888 1107 30-31 0.9583691084110626 1107 32-33 0.9540741660910399 1107 34-35 1.0457963056933366 1107 36-37 1.114637511692095 1107 38-39 1.1567466812061866 1107 40-41 1.1996376356900242 1107 42-43 1.2097012014713187 1107 44-45 1.1268923139868008 1107 46-47 1.3532337660442906 1107 48-49 1.2058844280553274 1107 50-51 1.2151564911498625 1107 52-53 1.1831655850541054 1107 54-55 1.2443414938576254 1107 56-57 1.1077783616528976 1107 58-59 1.2849671372406348 1107 60-61 1.446687747516556 1107 62-63 1.4252421548063055 1107 64-65 1.338700149446268 1107 66-67 1.5041032972735238 1107 68-69 1.6642132135880843 1107 70-71 1.6462269733382477 1107 72-73 1.5508224927823875 1107 74-75 1.5705260083261905 1107 76-77 1.517182125681181 1107 78-79 1.4626347898039747 1107 80-81 1.6170499342278397 1107 82-83 1.6689676718102469 1107 84-85 1.7562015618623406 1107 86-87 1.7120852649300282 1107 88-89 2.0821702322438824 1107 90-91 2.1235542114492425 1107 92-93 2.377640047758092 1107 94-95 2.3026504473048846 1107 96-97 2.4111273777915905 1107 98-99 2.3816463134736345 1107 100-101 2.329771741402684 1108 1 0.24539678481793814 1108 2 0.209738749687034 1108 3 0.2951426932385104 1108 4 0.2480560269182206 1108 5 0.2940572218532296 1108 6 0.040512250834368047 1108 7 0.1906568091427019 1108 8 0.2392867024123504 1108 9 0.10354277188069716 1108 10-11 0.302312223078367 1108 12-13 0.19829247395117733 1108 14-15 0.28336641406615826 1108 16-17 0.3129941059394312 1108 18-19 0.3179621880929133 1108 20-21 0.2547872426778923 1108 22-23 0.428418834361203 1108 24-25 0.4417698749518024 1108 26-27 0.2987907416518212 1108 28-29 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0.44617066623246515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6024.0 3 1262.0 4 1224.0 5 1423.0 6 1679.0 7 1994.0 8 2385.0 9 3129.0 10 3790.0 11 4655.0 12 6333.0 13 8085.0 14 9326.0 15 11191.0 16 14645.0 17 18960.0 18 16013.0 19 11280.0 20 11551.0 21 12225.0 22 13073.0 23 14572.0 24 16579.0 25 18921.0 26 22230.0 27 25467.0 28 30359.0 29 36459.0 30 44646.0 31 54921.0 32 69895.0 33 90163.0 34 120657.0 35 167350.0 36 232203.0 37 292681.0 38 201681.0 39 19886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36378440780558 18.685326090661057 14.466754535500433 22.484134966032933 2 15.3609782505014 19.787683171113315 40.80932861105227 24.042009967333016 3 19.245302417244567 24.742553451087478 29.348318197555194 26.66382593411276 4 12.539285143557235 16.245064968367483 36.69223117317176 34.523418714903514 5 13.749578025748946 37.79012471855479 33.33903192138426 15.121265334312003 6 32.21586804831263 35.69763073013028 17.288978874830477 14.797522346726616 7 29.63224745095952 33.00501452615761 19.585184433306555 17.77755358957632 8 27.298490789078723 34.69167473827863 18.98517402189083 19.02466045075182 9 27.371478196494927 16.896114804743668 19.89677773974447 35.83562925901694 10-11 25.583312752196303 26.76875529616101 26.308821044382835 21.339110907259847 12-13 26.3392511743148 24.03555780030366 27.455445979662336 22.169745045719207 14-15 23.123890885750168 25.286845494930336 26.010295922668547 25.578967696650945 16-17 22.17446169613372 29.114732122964497 26.330070998182965 22.380735182718826 18-19 22.115536069550515 26.96409987581592 29.409068811227502 21.511295243406067 20-21 23.171616945225555 25.98902431779476 29.239740930333873 21.599617806645806 22-23 22.650140769058904 25.985336994752338 29.261025878112378 22.10349635807638 24-25 22.487787760467434 26.217278531894788 29.447232542206724 21.847701165431054 26-27 22.106203228844034 26.65441045637725 29.326866691897006 21.912519622881714 28-29 21.824214017012544 26.59648514690613 29.736393755154904 21.842907080926423 30-31 21.84911424809966 26.306778829102896 29.681059993203903 22.16304692959354 32-33 21.930520930534858 26.398549076227663 29.421472000544714 22.24945799269277 34-35 22.530265302244512 26.522580369626937 28.98997851610735 21.9571758120212 36-37 22.599498354148604 26.131967638706065 29.086888205257686 22.18164580188764 38-39 22.050672951799065 26.434002087756305 29.227443538072468 22.28788142237216 40-41 22.16890550472936 26.405542628571926 29.271929800136558 22.153622066562157 42-43 22.40625844168342 26.24503710240735 28.857073809670418 22.49163064623881 44-45 22.18860353229354 26.682505752447344 28.652881422225303 22.476009293033812 46-47 22.385928735165535 26.944725689304335 28.182942286079243 22.486403289450884 48-49 22.629910171996258 26.80387165659551 28.139422965329953 22.426795206078285 50-51 22.639203001617915 26.994662249901282 27.800028718878085 22.566106029602725 52-53 22.800779039720872 27.22296235673135 27.55719912190882 22.419059481638957 54-55 22.415463571394312 27.046087148915625 27.700748392047892 22.83770088764217 56-57 22.597392552625394 27.466184860222505 27.64692415261054 22.289498434541564 58-59 22.622965730907524 26.84279209291878 27.824308947164226 22.70993322900946 60-61 22.632265552905757 26.957978129212062 28.24245150317197 22.167304814710207 62-63 22.395937101298983 27.112442503523727 28.11625642098125 22.375363974196038 64-65 22.340249455590104 26.98871643011893 27.942218766928217 22.728815347362747 66-67 22.334381051967256 27.342455497738538 27.648789451865785 22.674373998428422 68-69 22.137309169929022 27.108682725690414 27.990307908298856 22.763700196081707 70-71 22.355310664022497 27.206483369272988 27.88897585907118 22.549230107633335 72-73 22.459090997683735 27.09991501557532 27.75216351411679 22.688830472624154 74-75 22.17636709842267 27.6242466547728 27.488878121288966 22.71050812551556 76-77 22.384990185862197 27.407464932711896 27.494065726258572 22.713479155167335 78-79 22.53349426712059 27.245510556870716 27.548236045434816 22.672759130573883 80-81 22.27323240885921 27.903269918310826 27.408630267689528 22.41486740514044 82-83 22.52911792026919 27.65148386709614 27.27657701381742 22.54282119881725 84-85 22.417463445114315 27.34648803981675 27.243698206823165 22.99235030824577 86-87 22.52405362373491 27.19588402538704 27.531179305794307 22.748883045083744 88-89 22.525020788785934 27.227060564663024 27.351824215965788 22.896094430585254 90-91 22.422028963985284 27.37740116140569 27.625769964947448 22.57479990966157 92-93 22.557928017670754 26.977843499996908 27.63223674848258 22.83199173384976 94-95 22.30713117660052 27.09558784859436 27.578776927450793 23.018504047354323 96-97 22.41874581373297 27.17366453247615 27.510422161343566 22.897167492447316 98-99 22.502504658138143 27.19378040320819 27.205847317030003 23.097867621623664 100-101 22.58842381561506 26.61631480688056 27.800258925906046 22.995002451598335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2145.0 1 1272.0 2 418.0 3 423.0 4 366.0 5 365.5 6 614.5 7 1213.5 8 1987.5 9 2412.0 10 2211.0 11 1980.0 12 2080.5 13 2232.0 14 2371.0 15 2512.5 16 2644.5 17 2676.0 18 2707.5 19 2867.0 20 3126.0 21 3501.5 22 4019.5 23 4808.5 24 5618.5 25 6848.0 26 8825.5 27 10815.0 28 12208.0 29 13958.5 30 16540.0 31 19108.5 32 22226.5 33 25691.0 34 29612.5 35 32737.5 36 36369.0 37 40160.0 38 42239.5 39 43530.0 40 44861.5 41 49317.0 42 53832.5 43 56789.0 44 59564.0 45 62325.0 46 62738.0 47 62300.5 48 64984.0 49 64559.5 50 61102.0 51 57946.0 52 55813.0 53 55508.0 54 54790.5 55 50603.0 56 44354.5 57 39474.5 58 35780.0 59 31850.0 60 27327.0 61 23086.5 62 19009.5 63 14924.5 64 11585.0 65 9184.0 66 6900.5 67 5650.0 68 4341.0 69 2820.0 70 2196.5 71 1678.5 72 1334.5 73 1118.5 74 847.5 75 651.5 76 464.0 77 294.5 78 192.5 79 137.0 80 83.5 81 52.0 82 41.5 83 33.5 84 23.5 85 14.5 86 9.5 87 7.0 88 10.0 89 10.5 90 5.5 91 6.5 92 5.5 93 3.0 94 1.5 95 0.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31799036022229676 2 0.2744427293060731 3 0.2662273606367714 4 0.27419565054910167 5 0.27685174718654504 6 0.20729907709907303 7 0.1983424721588568 8 0.19593345427838488 9 0.20841093150544468 10-11 0.20754615585604452 12-13 0.20346935636601504 14-15 0.2054459864217869 16-17 0.19154780634214108 18-19 0.19584079974452057 20-21 0.1841354436329966 22-23 0.19457452111504173 24-25 0.1927214304377556 26-27 0.19380239999950585 28-29 0.20658872567278003 30-31 0.20285165947358635 32-33 0.20967721013492352 34-35 0.20309873823055785 36-37 0.1875327765413545 38-39 0.17468468117883745 40-41 0.17224477845374409 42-43 0.188490206724619 44-45 0.1904359519357694 46-47 0.19055949131425515 48-49 0.20566218033413697 50-51 0.2010603384855431 52-53 0.22206203282811904 54-55 0.19269054559313417 56-57 0.17153442702745106 58-59 0.17270805112306561 60-61 0.16378233102747083 62-63 0.16869302132227904 64-65 0.1678591305175003 66-67 0.16721054878045014 68-69 0.17011372417486506 70-71 0.17184327547366543 72-73 0.16915629399160056 74-75 0.1827147407804106 76-77 0.17795847470870957 78-79 0.18107784401547455 80-81 0.17239920267685124 82-83 0.15538165329044046 84-85 0.15516545937809043 86-87 0.15939668309122704 88-89 0.16436914307527808 90-91 0.17122557858123671 92-93 0.16597515499559273 94-95 0.16529568841392117 96-97 0.17107115435812953 98-99 0.1808616501031245 100-101 0.16211454941791334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1618917.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.75115333262529 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78001451626187 35.2308078604934 2 13.712700148279321 13.095944947695989 3 4.954790786875449 7.0979092378550614 4 2.3633404807863445 4.514089347009165 5 1.315798192596362 3.1415440624730047 6 0.7854779673821939 2.250448731591959 7 0.5488435967352536 1.8345540320334264 8 0.3837594771006712 1.4659966107105804 9 0.2817851676375558 1.2110010072048403 >10 1.633775068729245 14.4814070046174 >50 0.14505030690290802 4.8357003517215 >100 0.08946306359533351 7.71790877121852 >500 0.003640858982218099 1.1431601332079342 >1k 0.0013003067793636066 1.148810427712411 >5k 2.6006135587272134E-4 0.8307174744548885 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7643 0.47210573488325835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5736 0.35431093749710457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3844 0.23744268544959377 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2251 0.13904357048570123 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2141 0.1322489046689855 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2125 0.13126058964109957 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1796 0.11093836188019522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.0884844621435195E-5 0.0 24-25 0.0 0.0 0.0 1.8530906772861117E-4 0.0 26-27 0.0 0.0 0.0 3.3973329083578714E-4 0.0 28-29 0.0 0.0 0.0 9.883150278859262E-4 0.0 30-31 0.0 0.0 0.0 0.002409017880471945 0.0 32-33 0.0 0.0 0.0 0.0033973329083578714 0.0 34-35 3.0884844621435195E-5 0.0 0.0 0.004169454023893751 0.0 36-37 6.176968924287039E-5 0.0 0.0 0.005621041721101205 0.0 38-39 6.176968924287039E-5 0.0 0.0 0.007041744573687224 0.0 40-41 6.176968924287039E-5 0.0 0.0 0.009821380589616392 0.0 42-43 6.176968924287039E-5 0.0 0.0 0.013836410390402967 0.0 44-45 6.176968924287039E-5 0.0 0.0 0.023009209242969217 0.0 46-47 6.176968924287039E-5 0.0 0.0 0.04027383738635149 0.0 48-49 6.176968924287039E-5 0.0 0.0 0.05315281759348997 0.0 50-51 6.176968924287039E-5 0.0 0.0 0.07020125182452219 0.0 52-53 6.176968924287039E-5 0.0 0.0 0.09188241274876971 0.0 54-55 6.176968924287039E-5 0.0 0.0 0.11881399725866118 0.0 56-57 6.176968924287039E-5 0.0 0.0 0.1480310602705389 0.0 58-59 6.176968924287039E-5 0.0 0.0 0.1840427890991323 0.0 60-61 6.176968924287039E-5 0.0 0.0 0.2285787350432419 0.0 62-63 6.176968924287039E-5 0.0 0.0 0.2816388981028676 0.0 64-65 6.176968924287039E-5 0.0 0.0 0.3279661650350203 0.0 66-67 6.176968924287039E-5 0.0 0.0 0.3990939621981856 0.0 68-69 6.176968924287039E-5 0.0 0.0 0.48813496924178323 0.0 70-71 6.176968924287039E-5 0.0 0.0 0.5593554209388127 0.0 72-73 6.176968924287039E-5 0.0 0.0 0.6684098072971005 0.0 74-75 6.176968924287039E-5 0.0 0.0 0.7660368011454571 0.0 76-77 6.176968924287039E-5 0.0 0.0 0.8577339048264983 0.0 78-79 6.176968924287039E-5 0.0 0.0 0.9683943031051006 0.0 80-81 6.176968924287039E-5 0.0 0.0 1.0950839357422277 0.0 82-83 6.176968924287039E-5 0.0 0.0 1.2392852752797086 0.0 84-85 6.176968924287039E-5 0.0 0.0 1.4277137123150847 0.0 86-87 6.176968924287039E-5 0.0 0.0 1.6578366895893983 0.0 88-89 6.176968924287039E-5 0.0 0.0 1.9328971157879002 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5150 0.0 40.717968 1 GTATCAA 8500 0.0 38.09631 1 TATCAAC 10640 0.0 30.424656 2 ATCAACG 10585 0.0 30.35632 3 TCAACGC 10730 0.0 29.992254 4 CAACGCA 10985 0.0 29.380758 5 AACGCAG 11310 0.0 28.601933 6 GTACATG 9795 0.0 26.748652 1 TACATGG 10085 0.0 25.78289 2 ACGCAGA 12935 0.0 24.900097 7 CGCAGAG 12970 0.0 24.75965 8 GAGTACT 6255 0.0 24.452652 12-13 ACATGGG 10020 0.0 24.430523 3 GCAGAGT 13490 0.0 23.277016 9 GTACTTT 6665 0.0 22.59035 14-15 GTCCTAC 295 5.456968E-12 22.566322 1 AGTACTT 6500 0.0 22.288658 12-13 CATGGGA 5915 0.0 21.377014 4 CATGGGG 3990 0.0 20.72987 4 GTACCTG 1175 0.0 20.638924 1 >>END_MODULE