FastQCFastQC Report
Thu 26 May 2016
SRR937606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618917
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93820.57952322447661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75450.4660523053374571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.32527918355295543No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG25830.1595511073143342No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG22110.13657278291598643No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18310.11310030100369567No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17500.10809695617502318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.041.6261441
GTATCAA89300.036.020241
ATCAACG116500.027.3614373
TCAACGC117250.027.1864194
TATCAAC117600.027.1459032
CAACGCA118500.026.939735
AACGCAG120700.026.523326
GTACATG94050.026.1180741
TACATGG96650.025.2640882
ACATGGG96050.024.1359713
GAGTACT76850.023.17800712-13
AGTACTT78900.023.05740712-13
ACGCAGA139100.022.8789657
GGACCGA2704.0017767E-1122.8694046
CGCAGAG139200.022.7601578
GTACTTT81000.021.90185214-15
CATGGGG37850.021.7131374
CGGTTAC3650.021.47169528-29
GCAGAGT147400.021.2039649
ACTTGAC7900.021.0440658
Produced by FastQC (version 0.11.5)