##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937606_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1618917 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26142476729814 33.0 31.0 34.0 31.0 34.0 2 32.45731436509716 34.0 31.0 34.0 31.0 34.0 3 32.54812630913135 34.0 31.0 34.0 31.0 34.0 4 36.16375453466731 37.0 35.0 37.0 35.0 37.0 5 36.082787443704646 37.0 35.0 37.0 35.0 37.0 6 36.1207523301071 37.0 35.0 37.0 35.0 37.0 7 36.06937477338245 37.0 35.0 37.0 35.0 37.0 8 36.04870787075557 37.0 35.0 37.0 35.0 37.0 9 37.73132100039718 39.0 37.0 39.0 35.0 39.0 10-11 37.6626985818297 39.0 37.0 39.0 35.0 39.0 12-13 37.56620073790071 39.0 37.0 39.0 35.0 39.0 14-15 38.79461393017678 40.0 38.0 41.0 35.0 41.0 16-17 38.75833195895775 40.0 38.0 41.0 35.0 41.0 18-19 38.75747706645863 40.0 38.0 41.0 35.0 41.0 20-21 38.65417590895642 40.0 38.0 41.0 34.0 41.0 22-23 38.49609831757897 40.0 38.0 41.0 34.0 41.0 24-25 38.56425035996287 40.0 38.0 41.0 34.0 41.0 26-27 38.448156699818455 40.0 38.0 41.0 34.0 41.0 28-29 38.30482600405085 40.0 38.0 41.0 34.0 41.0 30-31 38.34051467740471 40.0 38.0 41.0 34.0 41.0 32-33 38.26029499968189 40.0 38.0 41.0 34.0 41.0 34-35 38.130164795353934 40.0 37.5 41.0 34.0 41.0 36-37 37.96227817732472 40.0 37.0 41.0 33.0 41.0 38-39 37.817970902770185 40.0 37.0 41.0 33.0 41.0 40-41 37.6304733349517 40.0 37.0 41.0 33.0 41.0 42-43 37.39836384447133 40.0 37.0 41.0 33.0 41.0 44-45 37.0594301622628 40.0 36.0 41.0 31.5 41.0 46-47 36.66940182850634 39.5 36.0 41.0 31.0 41.0 48-49 36.30607622256113 39.0 36.0 41.0 30.0 41.0 50-51 36.043915160567224 39.0 35.0 41.0 29.5 41.0 52-53 35.79059951807288 39.0 35.0 40.0 29.0 41.0 54-55 35.55933225730534 39.0 35.0 40.0 28.0 41.0 56-57 35.29381123306507 38.0 35.0 40.0 28.0 41.0 58-59 35.01912451348649 38.0 34.0 40.0 27.0 41.0 60-61 34.670269383791755 38.0 34.0 40.0 26.0 41.0 62-63 34.32083917829018 37.0 34.0 40.0 26.0 41.0 64-65 33.93637876432207 36.5 33.5 39.5 26.0 41.0 66-67 33.49669686586773 36.0 33.0 39.0 25.0 40.5 68-69 33.05800142935061 35.5 32.5 39.0 23.5 40.0 70-71 32.651836999673236 35.0 32.0 38.0 22.5 40.0 72-73 32.17511367167063 35.0 31.5 37.5 22.0 39.5 74-75 31.68185645094838 35.0 31.0 37.0 20.0 39.0 76-77 30.440477492051784 33.5 29.5 35.5 20.0 38.0 78-79 31.046826366021236 34.0 31.0 36.0 20.0 38.0 80-81 30.854613300125948 34.0 31.0 35.5 18.5 37.0 82-83 30.51083007961495 34.0 31.0 35.0 18.0 37.0 84-85 30.144233768624332 34.0 30.0 35.0 14.5 36.0 86-87 29.723431466838633 34.0 30.0 35.0 7.5 36.0 88-89 29.36920144763444 34.0 29.5 35.0 4.5 35.5 90-91 28.86548723622026 34.0 29.0 35.0 2.0 35.0 92-93 28.399827477257944 34.0 29.0 35.0 2.0 35.0 94-95 27.936599282112674 33.0 28.0 35.0 2.0 35.0 96-97 27.390666414646333 33.0 27.0 35.0 2.0 35.0 98-99 26.5260822512828 33.0 25.5 34.5 2.0 35.0 100-101 23.844191209308445 30.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3602517444464155 1101 2 0.2986634899562546 1101 3 0.23095702477388613 1101 4 0.20440591441813183 1101 5 0.19065055226059968 1101 6 0.22245595402876717 1101 7 0.24877695619891682 1101 8 0.21393280685698102 1101 9 0.3376320222162974 1101 10-11 0.3647447282982057 1101 12-13 0.3114265476328413 1101 14-15 0.25306686404546497 1101 16-17 0.23292078319304466 1101 18-19 0.39173791170978944 1101 20-21 0.39134263436394434 1101 22-23 0.3198717600672154 1101 24-25 0.36990874451604583 1101 26-27 0.28311758431652834 1101 28-29 0.2974250339610123 1101 30-31 0.4523285199218918 1101 32-33 0.49071005178601723 1101 34-35 0.4461421098592666 1101 36-37 0.4539900975848852 1101 38-39 0.25813751081425096 1101 40-41 0.30118328009510265 1101 42-43 0.5125993252017622 1101 44-45 0.6364463202022392 1101 46-47 0.5652598724579789 1101 48-49 0.6694305362948683 1101 50-51 0.4663154851823208 1101 52-53 0.48724606278845783 1101 54-55 0.5172003607308753 1101 56-57 0.478736027558611 1101 58-59 0.5699907260979487 1101 60-61 0.47111520126925654 1101 62-63 0.49698547783555114 1101 64-65 0.6271980710814802 1101 66-67 0.6590873781855677 1101 68-69 0.6544839261256854 1101 70-71 0.7153923616820634 1101 72-73 0.459960886605721 1101 74-75 0.5603074074142675 1101 76-77 0.4383104778081055 1101 78-79 0.4592222667530734 1101 80-81 0.4738890240903366 1101 82-83 0.45222663657549234 1101 84-85 0.3661229183652317 1101 86-87 0.22325398352730375 1101 88-89 0.32500178446811034 1101 90-91 0.5001734804195586 1101 92-93 0.572530330217635 1101 94-95 0.6256316343701336 1101 96-97 0.9892237635997994 1101 98-99 0.8331375209530059 1101 100-101 1.3386475878765083 1102 1 0.23151040289496905 1102 2 0.25046093373805434 1102 3 0.19070619481053797 1102 4 0.09811855458567464 1102 5 0.11697231241822692 1102 6 0.18403728201604252 1102 7 0.16919807313017543 1102 8 0.25803893806046574 1102 9 0.30031869220511 1102 10-11 0.29006582479134835 1102 12-13 0.28111154812656736 1102 14-15 0.3554177825739515 1102 16-17 0.2922215395562162 1102 18-19 0.4012316819134796 1102 20-21 0.27595055562440507 1102 22-23 0.41100142877846224 1102 24-25 0.38801541767391967 1102 26-27 0.44467280969836764 1102 28-29 0.4538173400458234 1102 30-31 0.47997565709376033 1102 32-33 0.45559381864459425 1102 34-35 0.4579692310021386 1102 36-37 0.4753262983774533 1102 38-39 0.43853579370678375 1102 40-41 0.37141670321833686 1102 42-43 0.4289448762384822 1102 44-45 0.3897133559796515 1102 46-47 0.36118821047217864 1102 48-49 0.20912276284710885 1102 50-51 0.22552430832224246 1102 52-53 0.3025865869685731 1102 54-55 0.3352640526190882 1102 56-57 0.2743675536883927 1102 58-59 0.31190580766284626 1102 60-61 0.09089075713610839 1102 62-63 0.1629259026573706 1102 64-65 0.14136811156731 1102 66-67 0.20641767871764927 1102 68-69 0.06705301956918674 1102 70-71 0.020935031787232106 1102 72-73 0.31647138161368815 1102 74-75 0.12063561897243957 1102 76-77 0.0956636429977955 1102 78-79 0.20515933115149565 1102 80-81 0.025377561525377956 1102 82-83 -0.08000855019592379 1102 84-85 0.00456776953062743 1102 86-87 0.010089276086986843 1102 88-89 0.04345667097535966 1102 90-91 0.20703337525897325 1102 92-93 0.14762029234971763 1102 94-95 0.19120505349760109 1102 96-97 0.37724090711743585 1102 98-99 0.4953594727083477 1102 100-101 1.1443967441346636 1103 1 0.2807193702494999 1103 2 0.25286554736614164 1103 3 0.2474112610234016 1103 4 0.11292158338196856 1103 5 0.10989604893059379 1103 6 0.22991747453467326 1103 7 0.1699880216059455 1103 8 0.2067879931504919 1103 9 0.31983187757793985 1103 10-11 0.2086235575756632 1103 12-13 0.24810830085139202 1103 14-15 0.3844862415351358 1103 16-17 0.40792039160743343 1103 18-19 0.2946873345126093 1103 20-21 0.1792835549836198 1103 22-23 0.26305918287232544 1103 24-25 0.2784920336288579 1103 26-27 0.06799038393969425 1103 28-29 0.1674871871928545 1103 30-31 0.3096139033109466 1103 32-33 0.3440665899094526 1103 34-35 0.47129909748477417 1103 36-37 0.4669372549216888 1103 38-39 0.3532032265200442 1103 40-41 0.3348063485752135 1103 42-43 0.47358593755837575 1103 44-45 0.49399683570081265 1103 46-47 0.5251914958802217 1103 48-49 0.4968425221763866 1103 50-51 0.45812618849681286 1103 52-53 0.38004864289827367 1103 54-55 0.46775818875641306 1103 56-57 0.2929319036464051 1103 58-59 0.3464719416408073 1103 60-61 0.3473453053775444 1103 62-63 0.47484694669035576 1103 64-65 0.4702270640799284 1103 66-67 0.34475511569561235 1103 68-69 0.3872571201233086 1103 70-71 0.5280069959812224 1103 72-73 0.3730327018781665 1103 74-75 0.38248035988952367 1103 76-77 0.2929028983152726 1103 78-79 0.3262913688148039 1103 80-81 0.2931930866859638 1103 82-83 0.3049965603557432 1103 84-85 0.4099455503324485 1103 86-87 0.46416814681582963 1103 88-89 0.43397814912485444 1103 90-91 0.49277919802787906 1103 92-93 0.5919035028977184 1103 94-95 0.7572414561776704 1103 96-97 0.7387090672964085 1103 98-99 0.5992023682938346 1103 100-101 1.0206892426523417 1104 1 0.2633837695296606 1104 2 0.23439310861270002 1104 3 0.11732664285081995 1104 4 0.09777435656705791 1104 5 0.07422402119992455 1104 6 0.09963787999118523 1104 7 0.15494417943931182 1104 8 0.10098697439894977 1104 9 0.2015527641858199 1104 10-11 0.28699403469385487 1104 12-13 0.25271284867523747 1104 14-15 0.2740566960109021 1104 16-17 0.25244855272081423 1104 18-19 0.17151219148407648 1104 20-21 0.19465720597119684 1104 22-23 0.2636433257900066 1104 24-25 0.22932437832192676 1104 26-27 0.18927394169239875 1104 28-29 0.15057770418685124 1104 30-31 0.2634538371447732 1104 32-33 0.37271219598329 1104 34-35 0.32847988853850296 1104 36-37 0.3304591021026724 1104 38-39 0.4432876166960611 1104 40-41 0.5076226328759219 1104 42-43 0.47662969167115676 1104 44-45 0.5453408754382565 1104 46-47 0.4781186572679843 1104 48-49 0.424420986407263 1104 50-51 0.4272527022170962 1104 52-53 0.5012286194539399 1104 54-55 0.477814016466489 1104 56-57 0.6514187612169593 1104 58-59 0.6329523744254502 1104 60-61 0.604557072516009 1104 62-63 0.6503390458232658 1104 64-65 0.5629965068799194 1104 66-67 0.4464067935537699 1104 68-69 0.6566220868004322 1104 70-71 0.6009126313486703 1104 72-73 0.5002400427385325 1104 74-75 0.5842376630518338 1104 76-77 0.6855493409006321 1104 78-79 0.9557059522611482 1104 80-81 0.8615806556844099 1104 82-83 0.9680889878036965 1104 84-85 1.089880044561383 1104 86-87 1.0509153355788996 1104 88-89 0.8938014203409175 1104 90-91 0.7676783881683669 1104 92-93 0.8841434040258314 1104 94-95 1.0194703269893104 1104 96-97 1.1544030141449078 1104 98-99 1.376147631036286 1104 100-101 1.5142453915230938 1105 1 0.25342552427729714 1105 2 0.21286932238302825 1105 3 0.2269313995143989 1105 4 0.09311261661120085 1105 5 -0.005243940620978549 1105 6 0.10642229854030916 1105 7 0.18891286046682154 1105 8 0.14558651899849906 1105 9 0.2839324936243699 1105 10-11 0.18651621362779025 1105 12-13 0.2370024064942058 1105 14-15 0.3703066304961382 1105 16-17 0.27375250049534117 1105 18-19 0.3188010446552809 1105 20-21 0.3005611059927489 1105 22-23 0.35590268275524295 1105 24-25 0.33882576723507185 1105 26-27 0.10901980261473199 1105 28-29 0.0846844029994358 1105 30-31 0.20498117573093566 1105 32-33 0.21967405765102654 1105 34-35 0.18692284992263808 1105 36-37 0.2094911723112176 1105 38-39 0.23426492237924634 1105 40-41 0.1952714969953746 1105 42-43 0.34087435003345945 1105 44-45 0.30590622423889613 1105 46-47 0.31417594920762326 1105 48-49 0.43250987684910314 1105 50-51 0.43702459434192775 1105 52-53 0.37992583638644817 1105 54-55 0.27974102427584313 1105 56-57 0.2647971687130095 1105 58-59 0.4111572114538191 1105 60-61 0.4133427489487431 1105 62-63 0.49270759407416875 1105 64-65 0.2939724731499993 1105 66-67 0.3597511333686967 1105 68-69 0.21892809028290827 1105 70-71 0.3769613044561666 1105 72-73 0.45600883674261894 1105 74-75 0.4354026612756563 1105 76-77 0.38264543505554727 1105 78-79 0.55842292262518 1105 80-81 0.48755828166203585 1105 82-83 0.6052871102959365 1105 84-85 0.6005604081829254 1105 86-87 0.7056379314779662 1105 88-89 0.567550961737517 1105 90-91 0.541415117787448 1105 92-93 0.5758964046023571 1105 94-95 0.5154156905817366 1105 96-97 0.5905712444307838 1105 98-99 0.7227455217518219 1105 100-101 0.9955707699072924 1106 1 0.12446520092865399 1106 2 0.1594676137260862 1106 3 0.19973976609349364 1106 4 0.04969280589315872 1106 5 0.13233842668957152 1106 6 0.09921666440470744 1106 7 0.17337256987029548 1106 8 0.17069815396106947 1106 9 0.10117834106350188 1106 10-11 -0.028748126317267975 1106 12-13 -0.01692372151220667 1106 14-15 0.09137778990361767 1106 16-17 0.17301417435577804 1106 18-19 0.20650102718580854 1106 20-21 0.19444117659120508 1106 22-23 0.21031284504539371 1106 24-25 0.32037012733915304 1106 26-27 0.3641480566183546 1106 28-29 0.35622474554354966 1106 30-31 0.2432678099917922 1106 32-33 0.18099383657212087 1106 34-35 0.09823489954432318 1106 36-37 0.1829717156327817 1106 38-39 0.22201743900981086 1106 40-41 0.31497572247139516 1106 42-43 0.06697793269988495 1106 44-45 0.05826009963421086 1106 46-47 0.15885997167747234 1106 48-49 0.0782630981236494 1106 50-51 0.2227150009198411 1106 52-53 0.20369047859110623 1106 54-55 0.2371318473980537 1106 56-57 -0.05399181554812316 1106 58-59 0.0013174991094402344 1106 60-61 0.11470482326647868 1106 62-63 0.14090339964101872 1106 64-65 0.36190568362447806 1106 66-67 0.23423946003192242 1106 68-69 0.1577109916482513 1106 70-71 0.32251435991735633 1106 72-73 0.2427469927957091 1106 74-75 0.15828402679359854 1106 76-77 0.009221615156182139 1106 78-79 3.2540274579773154E-4 1106 80-81 -0.006092591120399504 1106 82-83 -0.24344693938355988 1106 84-85 -0.1558150070312685 1106 86-87 -0.007466812887500396 1106 88-89 -0.04248263243698247 1106 90-91 -0.033666123642770884 1106 92-93 0.25605904246771516 1106 94-95 0.632608207949616 1106 96-97 0.7528452671561787 1106 98-99 0.729499756423408 1106 100-101 0.6112006701050738 1107 1 0.3230746759056444 1107 2 0.3754237406654326 1107 3 0.26237470986021094 1107 4 0.1979675845227149 1107 5 0.19445731684758272 1107 6 0.2478741193876388 1107 7 0.2802650409480236 1107 8 0.23501642610796125 1107 9 0.36076673348265587 1107 10-11 0.3221110380428982 1107 12-13 0.3271278319300066 1107 14-15 0.40909583933733984 1107 16-17 0.46194578673461706 1107 18-19 0.5456460431731713 1107 20-21 0.5350117179417353 1107 22-23 0.4805803693845121 1107 24-25 0.5050501830532497 1107 26-27 0.4895665389463204 1107 28-29 0.5848895317997815 1107 30-31 0.5363954350173117 1107 32-33 0.5121163001663902 1107 34-35 0.45320302327544937 1107 36-37 0.42314230424720023 1107 38-39 0.5018946155654973 1107 40-41 0.48034718260323217 1107 42-43 0.6490416458510637 1107 44-45 0.798796580551425 1107 46-47 0.45003527593874537 1107 48-49 0.46234841659988746 1107 50-51 0.3945883784616129 1107 52-53 0.41589190152053845 1107 54-55 0.4541453695243618 1107 56-57 0.299802543578636 1107 58-59 0.2879035963656307 1107 60-61 0.3687927466273777 1107 62-63 0.4570457624056772 1107 64-65 0.5914989635591681 1107 66-67 0.5683514987167015 1107 68-69 0.5005509604254357 1107 70-71 0.5563204365492922 1107 72-73 0.6917237027995427 1107 74-75 0.6961396696361639 1107 76-77 0.5931767789148061 1107 78-79 0.6867373486930965 1107 80-81 0.6219459933978122 1107 82-83 0.658452299023363 1107 84-85 0.6707193684946269 1107 86-87 0.7754339544345328 1107 88-89 0.9798607432786923 1107 90-91 0.9960620105436995 1107 92-93 1.1050925496987318 1107 94-95 1.225529187744307 1107 96-97 1.2830769228602499 1107 98-99 1.5040398286798684 1107 100-101 1.8057014094984467 1108 1 0.09698805050236103 1108 2 0.11521001574539724 1108 3 0.07575311443518729 1108 4 0.07162790936798302 1108 5 0.03451325780495296 1108 6 0.026973109957992847 1108 7 0.05951980769915366 1108 8 0.033961302110121494 1108 9 0.128464638466113 1108 10-11 0.1238581544527051 1108 12-13 0.16457019474311352 1108 14-15 0.2192998715172365 1108 16-17 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70-71 -0.007405081740820663 2316 72-73 -0.010772896697638146 2316 74-75 0.17762269004682452 2316 76-77 -0.01154342465686753 2316 78-79 0.022445780411768368 2316 80-81 0.012967519726874599 2316 82-83 0.0891632333592689 2316 84-85 0.14831043701667213 2316 86-87 0.128116721908917 2316 88-89 0.1362810072188445 2316 90-91 0.31053926920352026 2316 92-93 0.33611182176188237 2316 94-95 0.20120986142594077 2316 96-97 0.39188011780458254 2316 98-99 0.4732815165519142 2316 100-101 1.1821389816158536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 11.0 5 47.0 6 79.0 7 166.0 8 397.0 9 752.0 10 1178.0 11 2118.0 12 3229.0 13 4726.0 14 6364.0 15 8970.0 16 12617.0 17 18105.0 18 15692.0 19 9025.0 20 7577.0 21 7841.0 22 7820.0 23 8672.0 24 10150.0 25 11991.0 26 14780.0 27 18092.0 28 22920.0 29 29132.0 30 36424.0 31 46387.0 32 59246.0 33 79311.0 34 111647.0 35 161892.0 36 247846.0 37 361979.0 38 270465.0 39 21258.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47764304368214 19.198877533935633 15.06000016063473 22.263479261747495 2 15.7244009421113 20.22395218531895 39.88184693841624 24.16979993415351 3 19.40408309999833 24.539244445515116 29.872933572258493 26.18373888222806 4 12.902600138117283 16.78452345214137 36.299148688565296 34.013727721176046 5 13.695142324744586 37.842713734251355 33.3225623890984 15.139581551905653 6 32.407838079407405 35.89980215168536 16.839467372323597 14.852892396583641 7 29.586136905103842 33.208867409508954 19.375916121703586 17.82907956368362 8 27.603824037921648 34.41516767073296 18.849205981529625 19.13180230981576 9 27.40646988079068 16.45470397802976 19.83875640320041 36.30006973797916 10-11 25.69626836722844 26.801544984421678 26.096103812674652 21.40608283567523 12-13 26.320818994565936 23.970805435666882 27.51665829659459 22.19171727317259 14-15 23.05698809759858 25.257286198118866 26.14785069277795 25.537875011504607 16-17 22.114135560995408 28.980299793009774 26.60040632101584 22.305158324978983 18-19 22.09591350266876 26.529957990434344 29.866571294266475 21.507557212630417 20-21 22.883940313184677 25.61678578951237 30.03165078876805 21.4676231085349 22-23 22.500884858450444 25.62797153381024 30.025826130410532 21.845317477328784 24-25 22.44024863535314 25.849286899822538 30.185302890759687 21.525161574064636 26-27 22.084519831244478 26.327374591546164 30.004632746724646 21.58347283048471 28-29 21.675937899674253 26.279691135288758 30.412382755784574 21.631988209252416 30-31 21.805348884470295 25.88335288343998 30.361284735412625 21.950013496677098 32-33 21.94315706117114 26.00161095349545 30.113155893724013 21.94207609160939 34-35 22.400746919082327 26.079348107407608 29.724501009007874 21.795403964502196 36-37 22.58750757450814 25.72500628506588 29.756806556481898 21.930679583944084 38-39 21.983162786905297 26.062656818089163 29.965550980472095 21.98862941453345 40-41 22.004064463126426 26.10960461050954 29.90678913590008 21.979541790463955 42-43 22.269668245420732 25.87355820153625 29.524492619456023 22.332280933586997 44-45 22.132172310254326 26.22466130135146 29.341343626634348 22.30182276175987 46-47 22.359206803066495 26.680799571565434 28.811884735289084 22.148108890078984 48-49 22.697025233535754 26.603710999390334 28.50600123415839 22.193262532915522 50-51 22.669105333997976 26.737782109891985 28.182050098924154 22.411062457185885 52-53 22.903305110762318 26.95845432471214 27.8842893119289 22.253951252596643 54-55 22.44691976179137 26.843655357254264 27.99819261889275 22.71123226206161 56-57 22.555727069392688 27.287563228998152 27.996926340263272 22.159783361345887 58-59 22.83254793173461 26.61436627078473 28.097641818573777 22.455443978906885 60-61 22.65823386869123 26.64000069182052 28.72874273356818 21.97302270592007 62-63 22.39889382840504 26.83868289727021 28.557455385297704 22.204967889027046 64-65 22.35797140928164 26.685401413414027 28.310160434413874 22.646466742890464 66-67 22.44305915621369 27.051850094847357 28.009403817490337 22.495686931448617 68-69 22.2647164286418 26.790560459221567 28.37480198911183 22.569921123024795 70-71 22.40692388800661 27.024794970958983 28.21966783967306 22.348613301361343 72-73 22.570953297791053 26.890476781700357 28.03364842051816 22.504921499990427 74-75 22.405286991241677 27.283146696217287 27.682518622017067 22.629047690523972 76-77 22.443337119815286 27.172918685763385 27.913444605251534 22.470299589169798 78-79 22.574443285233276 26.96061626383564 27.861959569267604 22.60298088166348 80-81 22.25720103538385 27.665849350476073 27.804491460801096 22.272458153338977 82-83 22.44174414090309 27.413571738090393 27.596662242803088 22.54802187820343 84-85 22.350875513270523 27.131228930621422 27.629960328184282 22.88793522792377 86-87 22.42369435863605 26.900946744027028 27.789256645028743 22.88610225230818 88-89 22.358187603193986 27.017845880919154 27.758433570096553 22.865532945790303 90-91 22.366315776468117 27.03998171417275 27.942659105229993 22.651043404129137 92-93 22.36524674071601 26.660079789952963 28.0692060630975 22.905467406233523 94-95 22.172909420309995 26.64867933315914 28.07515765168937 23.10325359484149 96-97 22.260653263879497 26.708410622657 27.99121264400831 23.039723469455197 98-99 22.380077003236163 26.82697374069812 27.605530445804632 23.187418810261086 100-101 22.11549510877825 26.59000231061884 28.18173449630363 23.112768084299283 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 486.0 1 341.0 2 317.0 3 432.5 4 346.5 5 309.0 6 622.0 7 1466.5 8 2379.5 9 2797.5 10 2506.5 11 2157.5 12 2299.5 13 2507.5 14 2681.0 15 2860.0 16 2983.0 17 3063.0 18 3154.0 19 3246.5 20 3464.5 21 3829.5 22 4310.0 23 5009.5 24 5894.0 25 7127.5 26 8820.5 27 10702.0 28 12116.5 29 13872.0 30 16668.5 31 19241.0 32 21912.0 33 25052.0 34 29092.5 35 32365.5 36 35972.0 37 39866.5 38 41916.5 39 43041.5 40 44552.5 41 49161.0 42 53736.5 43 57084.5 44 59921.0 45 62327.0 46 62375.5 47 62324.5 48 65523.5 49 64972.0 50 61549.0 51 58453.0 52 55979.5 53 55473.5 54 54705.0 55 50354.5 56 43748.0 57 38850.5 58 35787.5 59 32037.0 60 27440.0 61 23050.0 62 19041.0 63 15044.5 64 11260.0 65 8834.5 66 6993.0 67 5876.0 68 4351.5 69 2606.0 70 1867.5 71 1412.5 72 1258.0 73 1091.5 74 885.0 75 766.5 76 509.0 77 263.0 78 153.5 79 107.5 80 70.5 81 47.5 82 34.5 83 24.0 84 12.0 85 3.5 86 2.5 87 3.0 88 2.0 89 1.5 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020878154964090193 2 0.0 3 0.0 4 1.8530906772861117E-4 5 0.02254593657364769 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.176968924287039E-5 12-13 0.003335563219115001 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.647756494001855E-4 24-25 0.0 26-27 4.3238782470009273E-4 28-29 0.001266278629478843 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.8530906772861117E-4 40-41 4.3238782470009273E-4 42-43 0.014546761816695977 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.1619391235004636E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.0884844621435195E-5 82-83 0.0028105208605506025 84-85 5.868120478072687E-4 86-87 0.0 88-89 0.0 90-91 0.01093323499598806 92-93 0.002563442103579121 94-95 0.0 96-97 0.0 98-99 0.0014824725418288894 100-101 0.018716215840589728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1618917.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.26182190900302 #Duplication Level Percentage of deduplicated Percentage of total 1 71.84833094247324 32.519863595773344 2 14.468860045948082 13.097739332519826 3 5.424377763138709 7.365516608470212 4 2.546452693784974 4.61028353303186 5 1.4376833491335008 3.253608385500976 6 0.8827451476072408 2.397279219722131 7 0.6031780838354859 1.9110657306982817 8 0.43798826918097206 1.5859317630321308 9 0.32017978240951894 1.3042728261254686 >10 1.7614003972273664 14.799953036591223 >50 0.16289103139248373 5.102122293962492 >100 0.09958534905702358 8.34700457592609 >500 0.0045390386690481585 1.3559380551851368 >1k 0.001375466263347927 0.9677712152365909 >5k 4.126398790043781E-4 1.3816498282242304 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9382 0.57952322447661 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7545 0.4660523053374571 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.32527918355295543 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2583 0.1595511073143342 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2211 0.13657278291598643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1831 0.11310030100369567 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1750 0.10809695617502318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.0884844621435195E-5 0.0 24-25 0.0 0.0 0.0 1.8530906772861117E-4 0.0 26-27 0.0 0.0 0.0 3.7061813545722234E-4 0.0 28-29 0.0 0.0 0.0 0.0010500847171287966 0.0 30-31 0.0 0.0 0.0 0.002409017880471945 0.0 32-33 0.0 0.0 0.0 0.0033046783744935656 0.0 34-35 0.0 0.0 0.0 0.004138569179272316 0.0 36-37 0.0 0.0 0.0 0.005713696254965511 0.0 38-39 0.0 0.0 0.0 0.007196168796794401 0.0 40-41 0.0 0.0 0.0 0.009975804812723567 0.0 42-43 0.0 0.0 0.0 0.014052604302753013 0.0 44-45 0.0 0.0 0.0 0.02313274862145496 0.0 46-47 0.0 0.0 0.0 0.040490031298701545 0.0 48-49 0.0 0.0 0.0 0.05367785995205437 0.0 50-51 0.0 0.0 0.0 0.07066452449384372 0.0 52-53 0.0 3.0884844621435195E-5 0.0 0.09237657026271268 0.0 54-55 0.0 6.176968924287039E-5 0.0 0.11964788806343994 0.0 56-57 0.0 6.176968924287039E-5 0.0 0.1491120298322891 0.0 58-59 0.0 6.176968924287039E-5 0.0 0.1852472980393683 0.0 60-61 0.0 6.176968924287039E-5 0.0 0.229937668206585 0.0 62-63 0.0 6.176968924287039E-5 0.0 0.2829360615769678 0.0 64-65 0.0 6.176968924287039E-5 0.0 0.3294177527322278 0.0 66-67 0.0 6.176968924287039E-5 0.0 0.4009470528754717 0.0 68-69 0.0 6.176968924287039E-5 0.0 0.49063664165611953 0.0 70-71 0.0 6.176968924287039E-5 0.0 0.5623203660224706 0.0 72-73 0.0 6.176968924287039E-5 0.0 0.672208643185537 0.0 74-75 0.0 6.176968924287039E-5 0.0 0.7707312975279152 0.0 76-77 0.0 6.176968924287039E-5 0.0 0.8636020253045709 0.0 78-79 0.0 6.176968924287039E-5 0.0 0.9753742779895449 0.0 80-81 0.0 6.176968924287039E-5 0.0 1.103268419566908 0.0 82-83 0.0 6.176968924287039E-5 0.0 1.2492610800924322 0.0 84-85 0.0 6.176968924287039E-5 0.0 1.4408088864345732 0.0 86-87 0.0 6.176968924287039E-5 0.0 1.6733099967447376 0.0 88-89 0.0 6.176968924287039E-5 0.0 1.9530031496364544 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5650 0.0 41.626144 1 GTATCAA 8930 0.0 36.02024 1 ATCAACG 11650 0.0 27.361437 3 TCAACGC 11725 0.0 27.186419 4 TATCAAC 11760 0.0 27.145903 2 CAACGCA 11850 0.0 26.93973 5 AACGCAG 12070 0.0 26.52332 6 GTACATG 9405 0.0 26.118074 1 TACATGG 9665 0.0 25.264088 2 ACATGGG 9605 0.0 24.135971 3 GAGTACT 7685 0.0 23.178007 12-13 AGTACTT 7890 0.0 23.057407 12-13 ACGCAGA 13910 0.0 22.878965 7 GGACCGA 270 4.0017767E-11 22.869404 6 CGCAGAG 13920 0.0 22.760157 8 GTACTTT 8100 0.0 21.901852 14-15 CATGGGG 3785 0.0 21.713137 4 CGGTTAC 365 0.0 21.471695 28-29 GCAGAGT 14740 0.0 21.203964 9 ACTTGAC 790 0.0 21.044065 8 >>END_MODULE