FastQCFastQC Report
Thu 26 May 2016
SRR937605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558662
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75940.4872127504231193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58920.37801652956189347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38580.24751998829765529No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21970.14095422869101834No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21870.14031265277526492No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG20450.1312022747715669No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17030.10926037845280119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53100.040.1113131
GTATCAA84100.037.3105241
ATCAACG107750.028.9317263
TATCAAC108900.028.673532
TCAACGC109300.028.4779624
CAACGCA110350.028.1639275
AACGCAG112900.027.6872676
ATGGGCG2007.1486284E-1026.1366845
GTACATG95150.025.732491
CCCTATA2701.8189894E-1224.6438162
TACATGG98000.024.5396352
ACGCAGA127050.024.4540757
CGCAGAG128800.023.9742838
ACATGGG99250.023.7486533
GAGTACT62050.022.96454212-13
GCAGAGT134200.022.5486779
AGTACTT63300.022.4735412-13
GTATAGG4550.021.9427621
GTACTTT67050.021.2173114-15
CATGGGG38400.021.0380524
Produced by FastQC (version 0.11.5)