##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558662 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.757678059771777 33.0 31.0 34.0 30.0 34.0 2 31.880979327140842 33.0 31.0 34.0 30.0 34.0 3 31.898885710949518 33.0 31.0 34.0 30.0 34.0 4 35.50777333379527 37.0 35.0 37.0 33.0 37.0 5 35.50354983954186 37.0 35.0 37.0 33.0 37.0 6 35.56728014155731 37.0 35.0 37.0 33.0 37.0 7 35.509498531432726 37.0 35.0 37.0 33.0 37.0 8 35.4864544076907 37.0 35.0 37.0 33.0 37.0 9 37.10032130121861 39.0 37.0 39.0 33.0 39.0 10-11 36.97966236425857 39.0 37.0 39.0 33.0 39.0 12-13 36.854552173595046 39.0 37.0 39.0 33.0 39.0 14-15 38.006171318733635 40.0 38.0 41.0 33.0 41.0 16-17 37.94109691517468 40.0 38.0 41.0 33.0 41.0 18-19 37.88737070641358 40.0 38.0 41.0 33.0 41.0 20-21 37.79498537848488 40.0 38.0 41.0 33.0 41.0 22-23 37.59819030681443 40.0 37.0 41.0 32.0 41.0 24-25 37.42453334975768 40.0 37.0 41.0 32.0 41.0 26-27 37.369972450730174 39.5 37.0 41.0 32.0 41.0 28-29 37.41604080936085 40.0 37.0 41.0 32.0 41.0 30-31 37.22788038715257 39.5 37.0 41.0 31.5 41.0 32-33 36.99260904545052 39.0 36.0 41.0 31.0 41.0 34-35 37.01259028577074 39.0 36.0 41.0 31.0 41.0 36-37 36.919202495473684 39.0 36.0 41.0 31.0 41.0 38-39 36.784036243906634 39.0 36.0 41.0 30.5 41.0 40-41 36.54047638294897 39.0 35.5 41.0 30.0 41.0 42-43 36.270006261780935 39.0 35.0 41.0 30.0 41.0 44-45 36.013777201214886 39.0 35.0 41.0 29.5 41.0 46-47 35.64441168130101 39.0 35.0 40.5 27.5 41.0 48-49 35.27108763798694 39.0 35.0 40.0 26.0 41.0 50-51 34.33007316531743 38.0 34.0 39.5 25.0 40.5 52-53 34.41230587516729 38.0 34.0 39.5 24.5 40.5 54-55 34.748952627317536 38.0 34.0 40.0 25.0 41.0 56-57 34.548276021356784 38.0 34.0 40.0 24.5 41.0 58-59 34.300984754873085 38.0 34.0 40.0 23.5 41.0 60-61 33.976188230674765 37.5 34.0 40.0 23.0 41.0 62-63 33.60153259654756 37.0 33.0 40.0 22.0 41.0 64-65 33.22535738986387 36.5 33.0 39.5 21.0 41.0 66-67 32.8117696460169 36.0 32.0 39.0 19.5 41.0 68-69 32.33584734855921 35.5 32.0 39.0 18.0 40.0 70-71 31.889059013435883 35.0 31.0 38.0 15.5 40.0 72-73 31.43220659771009 35.0 31.0 37.5 12.0 39.5 74-75 30.972790123836983 35.0 30.5 37.0 7.5 39.0 76-77 30.481752297804142 34.5 30.0 36.0 7.0 39.0 78-79 29.95811182924842 34.0 29.5 36.0 2.0 38.5 80-81 29.4376644840254 34.0 29.0 35.0 2.0 37.0 82-83 28.92079841556412 34.0 29.0 35.0 2.0 37.0 84-85 28.46675032816608 34.0 28.0 35.0 2.0 36.0 86-87 27.984195418891332 33.5 27.0 35.0 2.0 36.0 88-89 27.512393963540525 33.0 26.0 35.0 2.0 35.5 90-91 27.022014715185204 33.0 25.5 35.0 2.0 35.0 92-93 26.456352948875384 32.5 25.0 35.0 2.0 35.0 94-95 25.8060596203667 32.0 23.5 34.5 2.0 35.0 96-97 25.053628047645994 32.0 19.0 34.0 2.0 35.0 98-99 24.10462820034106 31.5 6.0 34.0 2.0 35.0 100-101 21.73195792288514 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22996939406261774 1101 2 0.34158233560637896 1101 3 0.2253886817833397 1101 4 0.1728093720732602 1101 5 0.21996180024956402 1101 6 0.13139182505195635 1101 7 0.20210165855609574 1101 8 0.17183707276486615 1101 9 0.2872434937123103 1101 10-11 0.2636610496981575 1101 12-13 0.3768439049875667 1101 14-15 0.5033577902408553 1101 16-17 0.6200179861557089 1101 18-19 0.48715036103075704 1101 20-21 0.7167887788679153 1101 22-23 0.655264781391871 1101 24-25 0.6245883192446158 1101 26-27 0.7422401173716437 1101 28-29 0.6002893719597537 1101 30-31 0.575421795297963 1101 32-33 0.4571079952930077 1101 34-35 0.6616979493334796 1101 36-37 0.5717581374610816 1101 38-39 0.7557625168191819 1101 40-41 0.7483127691701696 1101 42-43 0.8002746892212684 1101 44-45 0.7407819972802159 1101 46-47 0.790292260493132 1101 48-49 0.817597973104462 1101 50-51 0.8873367335888247 1101 52-53 0.9761594316360629 1101 54-55 0.9657733277219975 1101 56-57 0.9404298051291065 1101 58-59 0.8191221661089756 1101 60-61 1.0356874072622801 1101 62-63 1.252529081505564 1101 64-65 1.1593466779746038 1101 66-67 1.1086029094215561 1101 68-69 1.1098182144476212 1101 70-71 1.3634806854525543 1101 72-73 1.2030217023918937 1101 74-75 1.2115279089747304 1101 76-77 1.2884698862243589 1101 78-79 1.39905155378462 1101 80-81 1.3073840545465956 1101 82-83 1.2711703861563706 1101 84-85 1.562032495560704 1101 86-87 1.6211991935503782 1101 88-89 1.5927426562285767 1101 90-91 2.010322436372924 1101 92-93 1.9787843218720802 1101 94-95 1.9846515207586428 1101 96-97 2.1016930186340232 1101 98-99 2.3558796598388483 1101 100-101 2.6231385793120126 1102 1 0.2859189531789532 1102 2 0.2357979103353678 1102 3 0.2962445333683128 1102 4 0.20966266368856878 1102 5 0.21970817362587525 1102 6 0.18771036744947622 1102 7 0.2533792730436275 1102 8 0.25207315885569415 1102 9 0.3433147303988875 1102 10-11 0.2454738084605239 1102 12-13 0.3941730823207763 1102 14-15 0.44966874754308606 1102 16-17 0.4459831105196628 1102 18-19 0.3208866525512519 1102 20-21 0.5170748001693894 1102 22-23 0.5482015564621818 1102 24-25 0.6720117571890469 1102 26-27 0.4770711119672626 1102 28-29 0.35062398527791316 1102 30-31 0.5587476225474219 1102 32-33 0.49017585029127275 1102 34-35 0.6178592590266021 1102 36-37 0.7250667402233191 1102 38-39 0.6536596281620604 1102 40-41 0.4986066916125722 1102 42-43 0.6092732986204652 1102 44-45 0.7172504243210795 1102 46-47 0.7809953830240275 1102 48-49 0.8775083741663678 1102 50-51 0.8346511357885049 1102 52-53 0.7435853979456013 1102 54-55 0.8992646838738239 1102 56-57 0.881727883571628 1102 58-59 0.8117646236798493 1102 60-61 1.005855859586795 1102 62-63 0.8981554049466709 1102 64-65 0.957247344830904 1102 66-67 0.9721134653458279 1102 68-69 0.8241011068972952 1102 70-71 0.986311427211664 1102 72-73 0.9918328967283543 1102 74-75 1.001218500296119 1102 76-77 1.0362820603022946 1102 78-79 1.041849268774662 1102 80-81 1.1164648358114135 1102 82-83 0.9534319297232621 1102 84-85 0.9147619446931614 1102 86-87 1.097903864704893 1102 88-89 1.1799882394908003 1102 90-91 1.2663288837039204 1102 92-93 1.1736643825528397 1102 94-95 1.4755525668697054 1102 96-97 1.5937251130684622 1102 98-99 1.6311328914054855 1102 100-101 2.3237722508141765 1103 1 0.24072192556986494 1103 2 0.2292004119036939 1103 3 0.24376366053568077 1103 4 0.21073220056212705 1103 5 0.17058466030638186 1103 6 0.23122184374989985 1103 7 0.2726220441708165 1103 8 0.21479377609892225 1103 9 0.2989697752499083 1103 10-11 0.33874057666447754 1103 12-13 0.481358122709338 1103 14-15 0.5673946033342787 1103 16-17 0.32442171603113934 1103 18-19 0.3703246404314484 1103 20-21 0.17271141309244342 1103 22-23 0.3521784066073721 1103 24-25 0.4544042537775326 1103 26-27 0.3884325362055705 1103 28-29 0.12050257707861789 1103 30-31 0.16893215201586997 1103 32-33 0.21959676438554965 1103 34-35 0.2696397185960109 1103 36-37 0.2920211228184044 1103 38-39 0.3859815498684043 1103 40-41 0.3191476201937036 1103 42-43 0.30016905798356674 1103 44-45 0.23648389864249708 1103 46-47 0.2998877049942408 1103 48-49 0.43517039728065043 1103 50-51 0.36305004798138185 1103 52-53 0.1773444441418306 1103 54-55 0.41388896357348415 1103 56-57 0.41061144229188073 1103 58-59 0.42873460874028524 1103 60-61 0.782053449854736 1103 62-63 0.8026784222209429 1103 64-65 0.7435542008613751 1103 66-67 0.7545112166506129 1103 68-69 0.7665166919008186 1103 70-71 0.7590162479692459 1103 72-73 0.8007220982054974 1103 74-75 0.678468974029002 1103 76-77 0.7290711343727772 1103 78-79 0.6532270230544306 1103 80-81 0.6297657354188466 1103 82-83 0.7670604811030657 1103 84-85 0.97013089045155 1103 86-87 1.1186579730846269 1103 88-89 1.014165156896361 1103 90-91 0.9687389391290502 1103 92-93 1.1403139107601596 1103 94-95 1.400507133784071 1103 96-97 1.660462839668 1103 98-99 1.739627019057906 1103 100-101 2.392212909707098 1104 1 0.016631723275370547 1104 2 0.16840948806788347 1104 3 0.08175389540294731 1104 4 0.016162223949208965 1104 5 0.05613616589693038 1104 6 0.01527818375939205 1104 7 0.11374772231666697 1104 8 0.07261781446948135 1104 9 0.1801951311011578 1104 10-11 0.23115183260858885 1104 12-13 0.3686569607238539 1104 14-15 0.46417973775766086 1104 16-17 0.49229081200692804 1104 18-19 0.4138473898748245 1104 20-21 0.5775781954803634 1104 22-23 0.33762218759442675 1104 24-25 0.5104373758483902 1104 26-27 0.4774060249838783 1104 28-29 0.2903566024954287 1104 30-31 0.5135805461112426 1104 32-33 0.46178160188593864 1104 34-35 0.3697113954406177 1104 36-37 0.4788607182461604 1104 38-39 0.5129301594129743 1104 40-41 0.493986158890408 1104 42-43 0.7241306857513052 1104 44-45 0.7842107545996697 1104 46-47 0.6245256155137326 1104 48-49 0.5640195736249538 1104 50-51 0.558416759613543 1104 52-53 0.6349644630826035 1104 54-55 0.6919390184615324 1104 56-57 0.6331862570918076 1104 58-59 0.6396491667595896 1104 60-61 0.712514559723779 1104 62-63 0.7421625666497818 1104 64-65 0.7372039757408189 1104 66-67 0.6307022371161963 1104 68-69 0.758701320064624 1104 70-71 0.7893210671484958 1104 72-73 0.7616640793166383 1104 74-75 0.6814585097121011 1104 76-77 0.6621198536934543 1104 78-79 0.6779389968555343 1104 80-81 0.8244627793351462 1104 82-83 0.9143063653365893 1104 84-85 1.0036720531404058 1104 86-87 1.3286053961094737 1104 88-89 1.1833629121383602 1104 90-91 1.1413243882271864 1104 92-93 1.1329157467256898 1104 94-95 1.2418842251611615 1104 96-97 1.610411301002273 1104 98-99 1.7892672113461145 1104 100-101 2.336649748255841 1105 1 0.3165189176443768 1105 2 0.1698682788557555 1105 3 0.28875461546659764 1105 4 0.17180534842646722 1105 5 0.1398228396846548 1105 6 0.21345324125814358 1105 7 0.27425290404050173 1105 8 0.17885432955240788 1105 9 0.2334902578024156 1105 10-11 0.3741753967890915 1105 12-13 0.333644245675913 1105 14-15 0.4648451130262785 1105 16-17 0.31574196985972947 1105 18-19 0.30234581628184287 1105 20-21 0.2861561978180731 1105 22-23 0.41559284076897285 1105 24-25 0.3764801465005192 1105 26-27 0.3908605813287451 1105 28-29 0.40583436841441056 1105 30-31 0.5319330543763385 1105 32-33 0.47108188144772356 1105 34-35 0.6198362889393394 1105 36-37 0.3845920602859465 1105 38-39 0.5353988362103053 1105 40-41 0.5553237324538713 1105 42-43 0.6621003927301103 1105 44-45 0.6664211025957059 1105 46-47 0.7075228251043413 1105 48-49 0.9724213144515161 1105 50-51 0.940094546973846 1105 52-53 0.8063680403101898 1105 54-55 0.9279948926227064 1105 56-57 0.9860484775354479 1105 58-59 1.077761888187844 1105 60-61 1.0157594469029618 1105 62-63 0.997466796795841 1105 64-65 1.0634609512217637 1105 66-67 1.1866545245444229 1105 68-69 1.263723753150579 1105 70-71 1.4045963558575671 1105 72-73 1.4993341287054562 1105 74-75 1.5192145847879601 1105 76-77 1.4235448418551577 1105 78-79 1.5420166751863071 1105 80-81 1.6560661948413866 1105 82-83 1.6320461901514847 1105 84-85 1.791478541352241 1105 86-87 1.8456291069937194 1105 88-89 1.8924617561736135 1105 90-91 1.8506606492695745 1105 92-93 2.004913691137123 1105 94-95 2.1244726174226685 1105 96-97 2.281088087044413 1105 98-99 2.671928304427901 1105 100-101 2.672771248470905 1106 1 0.2624582356126268 1106 2 0.19027121773256894 1106 3 0.2375336401927548 1106 4 0.23854025054760086 1106 5 0.23312877123759534 1106 6 0.17667737993124177 1106 7 0.24354515622296447 1106 8 0.1687938591312701 1106 9 0.22908768117427059 1106 10-11 -0.005385663631123805 1106 12-13 -0.047544916274347315 1106 14-15 -0.0019374014815980445 1106 16-17 0.04087794760833674 1106 18-19 0.30286475543123714 1106 20-21 0.30091334774598266 1106 22-23 0.2666070922054118 1106 24-25 0.14380621334077404 1106 26-27 0.18955321333431385 1106 28-29 0.3367356592391175 1106 30-31 0.35553566220809074 1106 32-33 0.3439727244469921 1106 34-35 0.28054938978793587 1106 36-37 0.3046666888913947 1106 38-39 0.2053174468252621 1106 40-41 0.3737400870845562 1106 42-43 0.4631005666812058 1106 44-45 0.3648246529913166 1106 46-47 0.17324392411658351 1106 48-49 0.49014818612941013 1106 50-51 0.3464422823816804 1106 52-53 0.2158442054238492 1106 54-55 0.34075303963110315 1106 56-57 0.38876744968175103 1106 58-59 0.3576796828466442 1106 60-61 0.34017868338319346 1106 62-63 0.5595234008401135 1106 64-65 0.7408984633266087 1106 66-67 0.5470108014889092 1106 68-69 0.6722801742772049 1106 70-71 0.7714957493600387 1106 72-73 0.6357108888394478 1106 74-75 0.942335374992183 1106 76-77 0.6968260436599429 1106 78-79 0.7259789224192303 1106 80-81 0.6868284144573309 1106 82-83 0.40176229120810447 1106 84-85 0.5628326071452037 1106 86-87 0.8291254970602147 1106 88-89 0.8745683308076018 1106 90-91 0.8636991355005357 1106 92-93 1.110033053866399 1106 94-95 1.2906073434407297 1106 96-97 1.3042670701657784 1106 98-99 1.3932718793559928 1106 100-101 2.088293783207323 1107 1 0.23766745898372932 1107 2 0.2312297239138168 1107 3 0.2341321480250862 1107 4 0.2540307774499553 1107 5 0.13419784848770888 1107 6 0.1653661608116792 1107 7 0.2234973779636178 1107 8 0.21730478724717273 1107 9 0.36268621996443784 1107 10-11 0.37378912946734744 1107 12-13 0.16447110956512745 1107 14-15 0.35949016750276996 1107 16-17 0.48652953264951293 1107 18-19 0.4627355322665281 1107 20-21 0.5186140061159534 1107 22-23 0.6290349862470634 1107 24-25 0.6114888129734126 1107 26-27 0.6245256350625823 1107 28-29 0.5596997945867699 1107 30-31 0.5586983920700135 1107 32-33 0.6589990128089127 1107 34-35 0.5944753850823474 1107 36-37 0.5247917327483194 1107 38-39 0.586949518110842 1107 40-41 0.5673678036346175 1107 42-43 0.5331613730296212 1107 44-45 0.5291636774067285 1107 46-47 0.5870347368629538 1107 48-49 0.6673456764808847 1107 50-51 0.8042074104439223 1107 52-53 0.6836009748705294 1107 54-55 0.7377846991774746 1107 56-57 0.7807187994433775 1107 58-59 0.7291530694274613 1107 60-61 0.9511638133029123 1107 62-63 0.9338784459353562 1107 64-65 1.0422762159941428 1107 66-67 1.1525447725536466 1107 68-69 1.1255776997142277 1107 70-71 1.2196046125883235 1107 72-73 1.1962205580033825 1107 74-75 1.070593687170998 1107 76-77 1.1419700491910767 1107 78-79 1.1991101010872178 1107 80-81 1.2945790955297092 1107 82-83 1.1659783872186722 1107 84-85 1.1627290273875026 1107 86-87 1.3538691919810653 1107 88-89 1.4109882893240986 1107 90-91 1.581441715939313 1107 92-93 1.5083030268298891 1107 94-95 1.562741550816952 1107 96-97 1.9209484418042706 1107 98-99 2.1026855121547854 1107 100-101 2.8576114725144883 1108 1 0.14749783997936916 1108 2 0.08692536695689768 1108 3 0.13555239583586243 1108 4 0.1727877675795284 1108 5 0.23117933349647757 1108 6 0.19441213327606732 1108 7 0.193383045084488 1108 8 0.16380740259346993 1108 9 0.25200896493031877 1108 10-11 0.11899615940097164 1108 12-13 0.17055099606251645 1108 14-15 0.18481873411718652 1108 16-17 0.2880025269401898 1108 18-19 0.4818872663070479 1108 20-21 0.48746427937201986 1108 22-23 0.36423544729037616 1108 24-25 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-0.9350466087646119 2316 82-83 -0.942179289816039 2316 84-85 -0.9419327676383666 2316 86-87 -1.1077844333854223 2316 88-89 -1.099717780241587 2316 90-91 -1.1530859166280791 2316 92-93 -1.2076756277960676 2316 94-95 -0.8662705778796145 2316 96-97 -0.6151551121766303 2316 98-99 -0.541165843110214 2316 100-101 -0.29719974808842053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5832.0 3 1038.0 4 1119.0 5 1311.0 6 1424.0 7 1770.0 8 2076.0 9 2731.0 10 3381.0 11 4205.0 12 5673.0 13 7506.0 14 8329.0 15 10528.0 16 13476.0 17 17714.0 18 15033.0 19 10432.0 20 10527.0 21 10823.0 22 12081.0 23 13153.0 24 14948.0 25 17203.0 26 19939.0 27 23046.0 28 27939.0 29 33346.0 30 40568.0 31 50765.0 32 63911.0 33 84084.0 34 113018.0 35 159370.0 36 225409.0 37 293268.0 38 210049.0 39 21637.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4178324603287 18.716683182970616 14.458951622244243 22.40653273445644 2 15.448422905539037 19.784869723878188 40.73391862905436 24.032788741528417 3 19.238768437825872 24.75546500729182 29.386271044805305 26.619495510076995 4 12.56571207674283 16.229250806747803 36.73431320146215 34.470723915047216 5 13.701409921872537 37.753566933180345 33.36512802239821 15.179895122548908 6 32.153329745875546 35.71108492309789 17.30074018640636 14.834845144620196 7 29.540796778531774 33.11429166527382 19.56435093638063 17.78056061981378 8 27.248447628269172 34.70053916708779 18.962016559489044 19.08899664515399 9 27.320597111654326 16.880208621317077 19.923438651265243 35.87575561576335 10-11 25.555191214669648 26.79908314687653 26.31947123421547 21.326254404238355 12-13 26.2915375587194 24.077036899879097 27.48108265191019 22.150342889491313 14-15 23.098759701484862 25.28705851493347 26.060380653091876 25.553801130489795 16-17 22.164914817337603 29.147453327860113 26.306574007611296 22.381057847190995 18-19 22.137359429927763 26.907516271797522 29.383613251967915 21.571511046306796 20-21 23.076846431204757 26.021641685024967 29.31867840826326 21.58283347550702 22-23 22.588789825539447 25.945845011870567 29.368174664315372 22.097190498274614 24-25 22.4132090522658 26.287136318144448 29.492595394675526 21.80705923491423 26-27 22.137307414888404 26.684360346784985 29.309933618163655 21.868398620162953 28-29 21.804580407890338 26.59648818249621 29.814989841318827 21.783941568294626 30-31 21.86491827899196 26.27130140271336 29.70189930140786 22.161881016886824 32-33 21.874169189971678 26.477744819522748 29.424795759368806 22.223290231136776 34-35 22.44446223135998 26.546296394517217 29.050779822308716 21.958461551814086 36-37 22.540440644736208 26.126054606521286 29.13622703970945 22.197277709033056 38-39 22.051634286493023 26.487417019309824 29.229387651200504 22.231561042996645 40-41 22.129707864677425 26.394702952206817 29.338328615840297 22.137260567275458 42-43 22.358410724852618 26.29550237228993 28.89905883041172 22.447028072445725 44-45 22.10007453569469 26.687989210805522 28.70434206580547 22.507594187694313 46-47 22.35037475616502 26.932495677744733 28.27396171859105 22.443167847499197 48-49 22.578293385022636 26.837260659713685 28.15787739541193 22.42656855985175 50-51 22.591682215469763 27.014250461963407 27.864944660834635 22.5291226617322 52-53 22.816174052107215 27.15361928545087 27.634964621712594 22.39524204072932 54-55 22.399216975810145 27.09948360904721 27.690962595808767 22.81033681933388 56-57 22.482365681929924 27.586082787298793 27.633868174032646 22.29768335673864 58-59 22.617755282397365 26.818863983289148 27.88016389491444 22.68321683939905 60-61 22.55511005298895 26.958097334097786 28.27131577661853 22.21547683629473 62-63 22.376230686581135 27.17951206750203 28.128321483795098 22.31593576212174 64-65 22.28188264557627 26.982390569739543 28.00225601241771 22.73347077226647 66-67 22.28293496887088 27.33924763044599 27.737665656668238 22.64015174401489 68-69 22.17256610290098 27.113173206007975 27.99184546887108 22.722415222219972 70-71 22.306987255464055 27.25449349661011 27.931892449177404 22.506626798748428 72-73 22.42816565719674 27.137450530831416 27.81901145865673 22.615372353315117 74-75 22.200178180705574 27.586480635382628 27.536889576814396 22.676451607097402 76-77 22.384831931989783 27.415911351172046 27.47049078417595 22.728765932662224 78-79 22.55521153707685 27.191778444099285 27.53726843041855 22.71574158840532 80-81 22.234368350610765 27.85942218856591 27.51215934234768 22.394050118475644 82-83 22.474530194299174 27.63846359305137 27.32685080159538 22.560155411054076 84-85 22.363219073496097 27.357053072603254 27.310225533896613 22.969502320004036 86-87 22.54931485496009 27.147267213797072 27.55550984529398 22.74790808594886 88-89 22.47231639725536 27.23741236579254 27.425280307325593 22.8649909296265 90-91 22.407034568586916 27.38337215999671 27.649984057926297 22.55960921349008 92-93 22.536149227069117 26.99786169234532 27.680300989381102 22.785688091204463 94-95 22.30608452947739 27.024183006746018 27.62585747450356 23.043874989273032 96-97 22.397565553964057 27.172713842360686 27.52056530361641 22.909155300058842 98-99 22.40374951911318 27.25108864419325 27.274100504621817 23.07106133207175 100-101 22.45069597832413 26.64735866319714 27.881393325691118 23.020552032787613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2261.0 1 1338.5 2 409.0 3 420.5 4 391.0 5 367.0 6 582.0 7 1226.0 8 2039.5 9 2385.5 10 2133.5 11 1927.5 12 1986.0 13 2111.0 14 2284.0 15 2447.5 16 2544.0 17 2605.5 18 2687.5 19 2790.0 20 3025.0 21 3374.5 22 3886.5 23 4601.5 24 5469.5 25 6686.5 26 8434.5 27 10361.5 28 11712.5 29 13430.5 30 16117.5 31 18621.0 32 21571.0 33 24802.0 34 28387.5 35 31456.5 36 34928.5 37 38795.0 38 41087.5 39 41997.5 40 43201.0 41 47528.5 42 51630.5 43 54639.5 44 57647.5 45 60310.5 46 60478.5 47 60143.0 48 63416.0 49 62571.5 50 58333.5 51 55538.0 52 53572.5 53 53190.0 54 52736.0 55 48814.5 56 42547.5 57 37646.5 58 34095.5 59 30282.5 60 25895.0 61 21928.5 62 18132.5 63 14230.0 64 11157.0 65 8817.0 66 6692.0 67 5510.0 68 4121.5 69 2660.5 70 1991.0 71 1565.0 72 1312.0 73 1098.0 74 800.0 75 628.5 76 436.0 77 258.0 78 179.5 79 119.0 80 70.0 81 48.5 82 33.5 83 19.5 84 17.5 85 14.5 86 10.5 87 9.0 88 4.5 89 4.0 90 5.5 91 5.0 92 1.5 93 1.0 94 2.0 95 3.0 96 3.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29781954009272055 2 0.2789572081695711 3 0.2682428903764896 4 0.2542565354130658 5 0.2498938191859428 6 0.20806306947882222 7 0.19876021870039814 8 0.21172005219861648 9 0.21223331293121922 10-11 0.21441467104478074 12-13 0.2102765063881714 14-15 0.20722902078834285 16-17 0.2059779477526237 18-19 0.20594586895683603 20-21 0.19587312707950796 22-23 0.18756471897050161 24-25 0.18868747682307005 26-27 0.19991505534875426 28-29 0.21441467104478074 30-31 0.2083838574366989 32-33 0.2134843859669383 34-35 0.20604210534419906 36-37 0.1916387260355356 38-39 0.19433334488169982 40-41 0.18782134933680297 42-43 0.19872813990461047 44-45 0.19449373886063814 46-47 0.19571273310056958 48-49 0.20623457811892507 50-51 0.2145429862279314 52-53 0.2272141105640607 54-55 0.20238512262440478 56-57 0.1762409040574544 58-59 0.17880720772046793 60-61 0.18387565745491966 62-63 0.19090091373241921 64-65 0.18089232944666644 66-67 0.18503049410327577 68-69 0.19006686504193981 70-71 0.18336239672231697 72-73 0.18573622761060446 74-75 0.18849500404834402 76-77 0.20087741922238433 78-79 0.2046627171253293 80-81 0.19683549095313801 82-83 0.1953919451426929 84-85 0.18948944671776177 86-87 0.1905159681829672 88-89 0.19555233912163125 90-91 0.19439750247327517 92-93 0.19199159278919997 94-95 0.1926973262965287 96-97 0.18127727499611845 98-99 0.18878371321043305 100-101 0.18159806295399517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1558662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.12924768893306 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96581538349636 35.59919049106192 2 13.67803981337778 13.166275321542939 3 4.922211345816413 7.107069871202241 4 2.3555626997752266 4.53485842497175 5 1.2688558008367454 3.0534537560005623 6 0.7928606944023852 2.2895873246227114 7 0.5424397735537342 1.8275052752387506 8 0.3813105663884872 1.4681752556895082 9 0.27907624450451823 1.2088556726269681 >10 1.5742405288194996 14.102608103804664 >50 0.14635383282986103 4.891113369521493 >100 0.0880240857881501 7.590545512225702 >500 0.003472820273900532 1.0572515229125055 >1k 0.0014692701158809944 1.2365458496425905 >5k 2.671400210692717E-4 0.8669642489357929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7594 0.4872127504231193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5892 0.37801652956189347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3858 0.24751998829765529 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2197 0.14095422869101834 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2187 0.14031265277526492 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2045 0.1312022747715669 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1703 0.10926037845280119 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.2078795787669166E-5 0.0 24-25 0.0 0.0 0.0 9.62363873630075E-5 0.0 26-27 0.0 0.0 0.0 1.92472774726015E-4 0.0 28-29 0.0 0.0 0.0 7.057335073287216E-4 0.0 30-31 0.0 0.0 0.0 0.0016360185851711276 0.0 32-33 0.0 0.0 0.0 0.002630461254588872 0.0 34-35 0.0 0.0 0.0 0.0035286675366436085 0.0 36-37 0.0 0.0 0.0 0.00500429214287639 0.0 38-39 0.0 0.0 0.0 0.0069610986859242096 0.0 40-41 0.0 0.0 0.0 0.010201057060478795 0.0 42-43 0.0 0.0 0.0 0.013633488209759397 0.0 44-45 0.0 0.0 0.0 0.020626665691471275 0.0 46-47 0.0 0.0 0.0 0.0379812942126003 0.0 48-49 0.0 0.0 0.0 0.051935570380236384 0.0 50-51 0.0 0.0 0.0 0.068359913823523 0.0 52-53 0.0 0.0 0.0 0.09171327715694615 0.0 54-55 0.0 0.0 0.0 0.11808204729441021 0.0 56-57 0.0 0.0 0.0 0.14589436324231936 0.0 58-59 0.0 0.0 0.0 0.1788392865162556 0.0 60-61 0.0 0.0 0.0 0.2249365160631362 0.0 62-63 0.0 0.0 0.0 0.2819084573820366 0.0 64-65 0.0 0.0 0.0 0.32938507514778703 0.0 66-67 0.0 0.0 0.0 0.40101702614165224 0.0 68-69 0.0 0.0 0.0 0.4909980483260643 0.0 70-71 0.0 0.0 0.0 0.5632394964398952 0.0 72-73 0.0 0.0 0.0 0.6793968159870454 0.0 74-75 0.0 0.0 0.0 0.7776862462804637 0.0 76-77 0.0 0.0 0.0 0.8722545362625123 0.0 78-79 0.0 0.0 0.0 0.9869362312034295 0.0 80-81 0.0 0.0 0.0 1.111883140796401 0.0 82-83 0.0 0.0 0.0 1.2585473951376245 0.0 84-85 0.0 0.0 0.0 1.4528807400193242 0.0 86-87 0.0 0.0 0.0 1.693182999264754 0.0 88-89 0.0 0.0 0.0 1.9708570556028182 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5310 0.0 40.111313 1 GTATCAA 8410 0.0 37.310524 1 ATCAACG 10775 0.0 28.931726 3 TATCAAC 10890 0.0 28.67353 2 TCAACGC 10930 0.0 28.477962 4 CAACGCA 11035 0.0 28.163927 5 AACGCAG 11290 0.0 27.687267 6 ATGGGCG 200 7.1486284E-10 26.136684 5 GTACATG 9515 0.0 25.73249 1 CCCTATA 270 1.8189894E-12 24.643816 2 TACATGG 9800 0.0 24.539635 2 ACGCAGA 12705 0.0 24.454075 7 CGCAGAG 12880 0.0 23.974283 8 ACATGGG 9925 0.0 23.748653 3 GAGTACT 6205 0.0 22.964542 12-13 GCAGAGT 13420 0.0 22.548677 9 AGTACTT 6330 0.0 22.47354 12-13 GTATAGG 455 0.0 21.942762 1 GTACTTT 6705 0.0 21.21731 14-15 CATGGGG 3840 0.0 21.038052 4 >>END_MODULE