FastQCFastQC Report
Thu 26 May 2016
SRR937605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558662
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89140.5719007713025659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73470.4713658253040108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51600.3310531725287458No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG25650.16456422239074284No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG22070.1415958046067717No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.11689513185026644No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC17130.10990195436855457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53750.042.3471641
GTATCAA86750.036.810111
ATCAACG111750.028.4800823
TCAACGC113100.028.1401354
TATCAAC113600.027.9326522
CAACGCA114850.027.7527185
AACGCAG117600.027.2222986
GTACATG90500.026.2010841
TACATGG91750.025.5243592
ACATGGG92250.024.2016773
GGACCGA2801.8189894E-1223.7488296
ACGCAGA134400.023.6450827
CGCAGAG136050.023.253578
ATGGGAG24100.023.0612495
GAGTACT76150.022.67506212-13
AGTACTT78350.022.49307812-13
GTACTTT79100.021.61713814-15
TACACCG1100.002877036821.5919235
GCAGAGT145200.021.5264939
CATGGGA54100.020.8974694
Produced by FastQC (version 0.11.5)