##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1558662 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31836023461148 33.0 31.0 34.0 31.0 34.0 2 32.51921519867681 34.0 31.0 34.0 31.0 34.0 3 32.58259969127367 34.0 31.0 34.0 31.0 34.0 4 36.16826739857647 37.0 35.0 37.0 35.0 37.0 5 36.09491281624881 37.0 35.0 37.0 35.0 37.0 6 36.145822506739755 37.0 35.0 37.0 35.0 37.0 7 36.104868149733555 37.0 35.0 37.0 35.0 37.0 8 36.07052651569102 37.0 35.0 37.0 35.0 37.0 9 37.77692148778888 39.0 38.0 39.0 35.0 39.0 10-11 37.69394070042126 39.0 37.0 39.0 35.0 39.0 12-13 37.59200776050228 39.0 37.0 39.0 35.0 39.0 14-15 38.84254058930031 40.0 38.0 41.0 35.0 41.0 16-17 38.80555983272833 40.0 38.0 41.0 35.0 41.0 18-19 38.786916919768366 40.0 38.0 41.0 35.0 41.0 20-21 38.68913914626776 40.0 38.0 41.0 34.0 41.0 22-23 38.63892781116111 40.0 38.0 41.0 34.0 41.0 24-25 38.65063400531995 40.0 38.0 41.0 34.0 41.0 26-27 38.47912536521709 40.0 38.0 41.0 34.0 41.0 28-29 38.44075014339222 40.0 38.0 41.0 34.0 41.0 30-31 38.44425411025611 40.0 38.0 41.0 34.0 41.0 32-33 38.31375532347616 40.0 38.0 41.0 34.0 41.0 34-35 38.15875571483747 40.0 38.0 41.0 34.0 41.0 36-37 37.9856989520499 40.0 37.0 41.0 33.0 41.0 38-39 37.83619059167414 40.0 37.0 41.0 33.0 41.0 40-41 37.65532905787143 40.0 37.0 41.0 33.0 41.0 42-43 37.41079207679407 40.0 37.0 41.0 33.0 41.0 44-45 37.07758898337164 40.0 36.0 41.0 31.5 41.0 46-47 36.68729269078223 40.0 36.0 41.0 31.0 41.0 48-49 36.30881005631753 39.0 36.0 41.0 30.0 41.0 50-51 36.05458592048821 39.0 35.0 41.0 29.5 41.0 52-53 35.78743852098788 39.0 35.0 40.5 28.5 41.0 54-55 35.54331311086047 39.0 35.0 40.0 28.0 41.0 56-57 35.26762986458898 38.0 35.0 40.0 28.0 41.0 58-59 35.0108923551097 38.0 34.5 40.0 27.0 41.0 60-61 34.66503834699249 38.0 34.0 40.0 26.0 41.0 62-63 34.269942424977316 37.0 34.0 40.0 26.0 41.0 64-65 33.87310205804722 36.5 33.5 39.5 25.5 41.0 66-67 33.456961804419436 36.0 33.0 39.0 24.5 40.5 68-69 32.99439775910364 35.5 32.5 39.0 23.0 40.0 70-71 32.57710010252383 35.0 32.0 38.0 22.0 40.0 72-73 32.11521067428345 35.0 31.5 37.5 21.5 39.5 74-75 31.639358308600585 35.0 31.0 37.0 20.0 39.0 76-77 30.380220663620463 33.5 29.5 35.5 19.5 38.0 78-79 30.990287502999365 34.0 31.0 36.0 19.5 38.0 80-81 30.81759708005969 34.0 31.0 35.5 18.0 37.0 82-83 30.451300538538824 34.0 31.0 35.0 16.5 37.0 84-85 30.065446196802128 34.0 30.0 35.0 11.0 36.0 86-87 29.732721719012844 34.0 30.0 35.0 7.0 36.0 88-89 29.348994522224828 34.0 29.5 35.0 2.0 36.0 90-91 28.870522922865895 34.0 29.0 35.0 2.0 35.0 92-93 28.43304449585606 34.0 29.0 35.0 2.0 35.0 94-95 27.968634636630647 33.0 28.5 35.0 2.0 35.0 96-97 27.47368640539129 33.0 27.0 35.0 2.0 35.0 98-99 26.692874401249277 33.0 25.5 35.0 2.0 35.0 100-101 24.24572902912883 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32541143705223163 1101 2 0.29286903213829163 1101 3 0.20559106067289434 1101 4 0.18354205996296713 1101 5 0.2094615416013852 1101 6 0.1289901266615061 1101 7 0.13941755320662708 1101 8 0.17674494213672176 1101 9 0.28992503507652145 1101 10-11 0.341888840533521 1101 12-13 0.2688064774873169 1101 14-15 0.3297274502058656 1101 16-17 0.3793556780576779 1101 18-19 0.5294461190827846 1101 20-21 0.3661892775843043 1101 22-23 0.31939060114427775 1101 24-25 0.2541910443477491 1101 26-27 0.22671501119108228 1101 28-29 0.38215245371468853 1101 30-31 0.5338796911254278 1101 32-33 0.42342211700881904 1101 34-35 0.4520360023158503 1101 36-37 0.34080540859502406 1101 38-39 0.3067781765454427 1101 40-41 0.19550463811193453 1101 42-43 0.3754269266803547 1101 44-45 0.38370223679595483 1101 46-47 0.28126132424886663 1101 48-49 0.365932166989559 1101 50-51 0.34751429880797247 1101 52-53 0.3400924667649079 1101 54-55 0.43330856125843553 1101 56-57 0.46011857488652197 1101 58-59 0.47458665554561463 1101 60-61 0.576115685430949 1101 62-63 0.32778641159749355 1101 64-65 0.49762212616631274 1101 66-67 0.5035284010571957 1101 68-69 0.45695136296205163 1101 70-71 0.568659835821542 1101 72-73 0.47457391806398874 1101 74-75 0.6732494205195643 1101 76-77 0.48894544367979265 1101 78-79 0.40535122782021915 1101 80-81 0.5758060863034764 1101 82-83 0.5671396657927126 1101 84-85 0.426007729982139 1101 86-87 0.5202872234613416 1101 88-89 0.7484718888076749 1101 90-91 0.7691477313393413 1101 92-93 0.8689817328532747 1101 94-95 0.7890879700925346 1101 96-97 0.7586295535029457 1101 98-99 1.0668058959038405 1101 100-101 1.6531429508080464 1102 1 0.21344910969216357 1102 2 0.20079229625763162 1102 3 0.17258799503022004 1102 4 0.1278445472421268 1102 5 0.11048948003569592 1102 6 0.16383257791146377 1102 7 0.10584560541736465 1102 8 0.12180672905875412 1102 9 0.2628604669528798 1102 10-11 0.2975824026838936 1102 12-13 0.22880260592826573 1102 14-15 0.1684264483411937 1102 16-17 0.30755010933354754 1102 18-19 0.3751830584968303 1102 20-21 0.4320986198933312 1102 22-23 0.4359901081130886 1102 24-25 0.40508110787292395 1102 26-27 0.24312410066360712 1102 28-29 0.26462693302950413 1102 30-31 0.32413837447929694 1102 32-33 0.2538829905589566 1102 34-35 0.22202707402660593 1102 36-37 0.3008584252506239 1102 38-39 0.16090020176588382 1102 40-41 0.4096069894914791 1102 42-43 0.32486357533241517 1102 44-45 0.26572042522236927 1102 46-47 0.29236400747632274 1102 48-49 0.1792183305115671 1102 50-51 0.26258295972260726 1102 52-53 0.23698494803920056 1102 54-55 0.38848696902051216 1102 56-57 0.30630982990375344 1102 58-59 0.17504215631992537 1102 60-61 0.3243090895585041 1102 62-63 0.3660445260692242 1102 64-65 0.45293169286774315 1102 66-67 0.39339281747893295 1102 68-69 0.455484911206419 1102 70-71 0.2962431138468915 1102 72-73 0.5552299497949704 1102 74-75 0.4455025888770727 1102 76-77 0.43420199372561896 1102 78-79 0.47367555385289606 1102 80-81 0.4572411705296382 1102 82-83 0.5910133581538837 1102 84-85 0.5683211050334975 1102 86-87 0.49268024201321836 1102 88-89 0.752455249004889 1102 90-91 0.7721146097565814 1102 92-93 1.0413601342944396 1102 94-95 1.0554539983470157 1102 96-97 0.9998924718683604 1102 98-99 1.2150569783601455 1102 100-101 1.8743812176137737 1103 1 0.19310284813987266 1103 2 0.18511545646018845 1103 3 0.10953948348924314 1103 4 0.02206710792197697 1103 5 0.06026166787320619 1103 6 0.12806008610882458 1103 7 0.13751133431927087 1103 8 0.11001999616649272 1103 9 0.0644181265507413 1103 10-11 0.21907370734102471 1103 12-13 0.2394312801875884 1103 14-15 0.19281722461639106 1103 16-17 0.26008951162170035 1103 18-19 0.11249776911556353 1103 20-21 0.2965637221096955 1103 22-23 0.23073199644793618 1103 24-25 0.22014621785009325 1103 26-27 0.23880068176731584 1103 28-29 0.2365998930677904 1103 30-31 0.1473592083785249 1103 32-33 0.12182186446516852 1103 34-35 0.18989690904468404 1103 36-37 0.2067775802275733 1103 38-39 0.21236327647016395 1103 40-41 0.18983090507971667 1103 42-43 0.18427081099593323 1103 44-45 0.09308884712596921 1103 46-47 0.12671918102747526 1103 48-49 0.06121640000963424 1103 50-51 0.21675011144001388 1103 52-53 -0.04880775421079164 1103 54-55 0.1150379995916424 1103 56-57 0.17314521338485633 1103 58-59 0.1822746670072135 1103 60-61 0.24810398100415654 1103 62-63 0.13838838823721034 1103 64-65 0.25061379708250797 1103 66-67 0.313724850876298 1103 68-69 0.32537005119205986 1103 70-71 0.2764291954776965 1103 72-73 0.21828096743141145 1103 74-75 0.34269163901838695 1103 76-77 0.19983881683731752 1103 78-79 0.3880398463578949 1103 80-81 0.4053403375358364 1103 82-83 0.5091444738352529 1103 84-85 0.432035995444366 1103 86-87 0.41578636141330705 1103 88-89 0.40796534452754685 1103 90-91 0.6372179579001305 1103 92-93 0.7794222272560312 1103 94-95 0.7890976833097945 1103 96-97 0.9052639237222238 1103 98-99 0.843650800107234 1103 100-101 1.3653026846658918 1104 1 0.1809525344280729 1104 2 0.19985233294071492 1104 3 0.062346645144813806 1104 4 0.02398881771616601 1104 5 0.13459708703621232 1104 6 0.18238418434964387 1104 7 0.17379187547945207 1104 8 0.07409909524884739 1104 9 0.13006817105569723 1104 10-11 0.14049001164607233 1104 12-13 0.17295958961049962 1104 14-15 0.20787752611130372 1104 16-17 0.17983279798828278 1104 18-19 0.1804220462135575 1104 20-21 0.1175989501065331 1104 22-23 0.15520712684369187 1104 24-25 0.09267510420461633 1104 26-27 0.16782323066625082 1104 28-29 0.22464865844251136 1104 30-31 0.2681455770503831 1104 32-33 0.1929515032590885 1104 34-35 0.2396851022941604 1104 36-37 0.2882896853245782 1104 38-39 0.26338195013034493 1104 40-41 0.10812662900328718 1104 42-43 0.1504215048629618 1104 44-45 0.1699199769824702 1104 46-47 0.18649879985068196 1104 48-49 0.07841319062867314 1104 50-51 0.059507358881710104 1104 52-53 -0.04156010317654335 1104 54-55 0.004670521787609516 1104 56-57 0.0389301125139383 1104 58-59 -0.010329848466533065 1104 60-61 -0.04036663944435048 1104 62-63 -0.014785698573838602 1104 64-65 0.2335687754831568 1104 66-67 0.18189534965837595 1104 68-69 0.14668244081934745 1104 70-71 0.2683779013170948 1104 72-73 0.154166197396016 1104 74-75 0.28039537584378493 1104 76-77 0.17291855146552493 1104 78-79 0.26662860799805443 1104 80-81 0.1786145877131453 1104 82-83 0.1509501190566418 1104 84-85 0.05374574776569929 1104 86-87 0.11588470773807202 1104 88-89 0.01795790051880175 1104 90-91 0.14216876799082456 1104 92-93 0.3554488766398691 1104 94-95 0.4197320819988448 1104 96-97 0.4276926634574032 1104 98-99 0.840932983303535 1104 100-101 0.9591936990188472 1105 1 0.14102394182480538 1105 2 0.19491735785648245 1105 3 0.16563780042812226 1105 4 0.16453274138160623 1105 5 0.12983529181641273 1105 6 0.12041921355800866 1105 7 0.10894230975291919 1105 8 0.05399938010585004 1105 9 0.22117857517243067 1105 10-11 0.19087558641441404 1105 12-13 0.1291936958005806 1105 14-15 0.2801940086242638 1105 16-17 0.34020169280125856 1105 18-19 0.21870499606995253 1105 20-21 0.40474223322349445 1105 22-23 0.37125725438480117 1105 24-25 0.2429351127203816 1105 26-27 0.3155350211767711 1105 28-29 0.29631430585092033 1105 30-31 0.3329604199970859 1105 32-33 0.35836952429502134 1105 34-35 0.3322406251042054 1105 36-37 0.2911721100857392 1105 38-39 0.22560204654682536 1105 40-41 0.22888160640527389 1105 42-43 0.33447090148955283 1105 44-45 0.2690461348244142 1105 46-47 0.46372699318538224 1105 48-49 0.491551552659665 1105 50-51 0.426457913209191 1105 52-53 0.54469301807584 1105 54-55 0.6384098656920045 1105 56-57 0.6042414912122922 1105 58-59 0.6055441911773372 1105 60-61 0.6108805811056328 1105 62-63 0.5994576725219716 1105 64-65 0.7307416901523638 1105 66-67 0.667992131594545 1105 68-69 0.7084349516018591 1105 70-71 0.7954961753518788 1105 72-73 0.7069619671197458 1105 74-75 0.7653571569140531 1105 76-77 0.8506175856948062 1105 78-79 0.9124286924612157 1105 80-81 0.8819223231238276 1105 82-83 0.8547882449293276 1105 84-85 0.9437784870067389 1105 86-87 1.0766510047662763 1105 88-89 1.1415414823731993 1105 90-91 1.0213766806178946 1105 92-93 1.0888603940991324 1105 94-95 1.1184811504517675 1105 96-97 1.3184222454862855 1105 98-99 1.5061218339925446 1105 100-101 1.6697439552512598 1106 1 -0.35036458242433355 1106 2 -0.27104606220133576 1106 3 -0.2799029543402938 1106 4 -0.22627484001674958 1106 5 -0.18184247544061094 1106 6 -0.11020900095059005 1106 7 -0.15441740317398 1106 8 -0.2818952485713311 1106 9 -0.5303529117686807 1106 10-11 -0.5130652576608412 1106 12-13 -0.6125817351318688 1106 14-15 -0.5144587437482926 1106 16-17 -0.407581327420111 1106 18-19 -0.4749716127086572 1106 20-21 -0.476425857331499 1106 22-23 -0.3613534645891363 1106 24-25 -0.43032914230268915 1106 26-27 -0.5308701987906588 1106 28-29 -0.483014162231747 1106 30-31 -0.5038777140277446 1106 32-33 -0.5949107091222388 1106 34-35 -0.8306354320521763 1106 36-37 -0.6485255916078572 1106 38-39 -0.5341999630366203 1106 40-41 -0.8111473196888355 1106 42-43 -0.9207158000390621 1106 44-45 -0.9894971951374885 1106 46-47 -0.9404786331461494 1106 48-49 -0.9246123146497212 1106 50-51 -0.9349149464750468 1106 52-53 -0.9519064173900986 1106 54-55 -1.193939612813388 1106 56-57 -1.1110913561339686 1106 58-59 -1.193009459284987 1106 60-61 -1.1378082849484414 1106 62-63 -1.2308512430992025 1106 64-65 -1.5736106289564376 1106 66-67 -1.6855654820833976 1106 68-69 -1.5097393958125451 1106 70-71 -1.420212146324939 1106 72-73 -1.421007082138896 1106 74-75 -1.636414050772789 1106 76-77 -1.4967869564013583 1106 78-79 -1.44773256199516 1106 80-81 -1.5431303105315841 1106 82-83 -1.8292668061082829 1106 84-85 -1.6241989549438074 1106 86-87 -1.542571622146287 1106 88-89 -1.6587303834585043 1106 90-91 -2.246233177567131 1106 92-93 -2.162098354385517 1106 94-95 -2.3129218189113203 1106 96-97 -2.398036352360311 1106 98-99 -2.401810963499692 1106 100-101 -1.6528230159194877 1107 1 0.1698096069963455 1107 2 0.17221209518829994 1107 3 0.08262361780178651 1107 4 0.06777171024845785 1107 5 0.10748300492180363 1107 6 -0.031492254825479904 1107 7 -0.013475407838292597 1107 8 0.021423173404606644 1107 9 0.1614360383479223 1107 10-11 0.11954972489957782 1107 12-13 0.25266009715920745 1107 14-15 0.17357334404813685 1107 16-17 0.2461152961119737 1107 18-19 0.30354829317952436 1107 20-21 0.1870315137291101 1107 22-23 0.2293671822232355 1107 24-25 0.13683388165868138 1107 26-27 0.2376464565248 1107 28-29 0.1992404195278894 1107 30-31 0.2566950317258616 1107 32-33 0.20684176234495766 1107 34-35 0.24112870019969534 1107 36-37 0.2842094815558269 1107 38-39 0.24869080224106455 1107 40-41 0.3431452421752468 1107 42-43 0.371720942783881 1107 44-45 0.46602155499149944 1107 46-47 0.21576620721461381 1107 48-49 0.39718858003171675 1107 50-51 0.31814407463819805 1107 52-53 0.35639879590939216 1107 54-55 0.37960137020107965 1107 56-57 0.3631304412779599 1107 58-59 0.4709464619170092 1107 60-61 0.3231261515157655 1107 62-63 0.4053658126042663 1107 64-65 0.29303913989172514 1107 66-67 0.5213044485710583 1107 68-69 0.40497886208686396 1107 70-71 0.37546098021005747 1107 72-73 0.5496158669045101 1107 74-75 0.5355238323304228 1107 76-77 0.31671014388139085 1107 78-79 0.4848928688347165 1107 80-81 0.7246846338475059 1107 82-83 0.7810914276440108 1107 84-85 0.6698662990136341 1107 86-87 0.829359037461991 1107 88-89 0.8637243665053802 1107 90-91 0.9254086712347984 1107 92-93 0.925119542339143 1107 94-95 0.946484848374503 1107 96-97 0.5587110368622348 1107 98-99 0.8723322784048335 1107 100-101 1.1919447229202156 1108 1 0.13596883299059215 1108 2 0.12828599886736924 1108 3 0.1094510635534931 1108 4 0.03407257030912092 1108 5 -0.004233306927105218 1108 6 0.05505714860206723 1108 7 7.527109194285231E-4 1108 8 -0.027991242989784837 1108 9 0.08431506820340928 1108 10-11 0.03356991019600741 1108 12-13 0.12801479281690575 1108 14-15 0.1469162254711165 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quality scores pass #Quality Count 2 2.0 3 5.0 4 15.0 5 39.0 6 67.0 7 172.0 8 360.0 9 729.0 10 1169.0 11 2045.0 12 3233.0 13 4790.0 14 6275.0 15 8698.0 16 12237.0 17 17723.0 18 15750.0 19 9132.0 20 7707.0 21 7996.0 22 8062.0 23 8861.0 24 9769.0 25 11722.0 26 14138.0 27 17147.0 28 21388.0 29 26661.0 30 33898.0 31 42523.0 32 54697.0 33 73250.0 34 103038.0 35 150911.0 36 235407.0 37 355895.0 38 272290.0 39 20861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51601716764642 19.170538501085012 15.042870978860073 22.270573352408498 2 15.744465445362755 20.19276789964726 39.86233063999764 24.200436014992345 3 19.38630697354526 24.58685718905061 29.847330595087325 26.179505242316807 4 12.972023440617658 16.758989440943576 36.350664865121495 33.91832225331727 5 13.639093498960552 37.89171778353823 33.33566460488156 15.133524112619664 6 32.3739848665073 35.946856983746315 16.850863112079466 14.828295037666923 7 29.619506987403298 33.222789803049025 19.38380482747382 17.773898382073856 8 27.598222064822263 34.41214323567265 18.81376462632694 19.17587007317815 9 27.404722768631046 16.592949593946603 19.81853666798831 36.183790969434035 10-11 25.668650419398176 26.820182951788134 26.10665429708301 21.404512331730675 12-13 26.363425507840933 24.0288056430727 27.523292784762575 22.08447606432379 14-15 23.059136618458652 25.252011019707933 26.15935975856215 25.52949260327127 16-17 22.122660333029227 28.98511672190635 26.629153722872566 22.263069222191852 18-19 22.01186017237862 26.593482101956678 29.894518503690986 21.50013922197372 20-21 22.807184128910897 25.68000805821418 30.044820608497822 21.4679872043771 22-23 22.516617050762203 25.615151670687265 30.077054355078786 21.791176923471745 24-25 22.415154793021195 25.917325244344187 30.1507318456471 21.516788116987517 26-27 22.075759442181795 26.335038661811165 30.025810897164458 21.563390998842582 28-29 21.695365886989723 26.322581845548388 30.38577937005217 21.596272897409722 30-31 21.864850065264328 25.88576801313178 30.364675075377175 21.884706846226713 32-33 21.882101443417497 26.099308252847635 30.08233985302779 21.93625045070708 34-35 22.422693310031296 26.05860026099308 29.729473099363428 21.78923332961219 36-37 22.629824229921237 25.712641440010316 29.753624197264887 21.90391013280356 38-39 22.010929887300776 26.079483557050853 29.956590973540127 21.952995582108244 40-41 21.99240564883199 26.10231811522537 29.928525074607425 21.976751161335212 42-43 22.277010112729435 25.908807681028378 29.518177437043303 22.29600476919888 44-45 22.089362543001627 26.266406700105605 29.338015554366503 22.306215202526268 46-47 22.372746624989894 26.715477762337187 28.735319139107773 22.17645647356515 48-49 22.690230466900456 26.552677873714764 28.574957238965215 22.182134420419565 50-51 22.672907917175117 26.75272124424667 28.176731068057094 22.397639770521128 52-53 22.917284183485577 26.967809570002988 27.890780682405808 22.224125564105623 54-55 22.458012064193518 26.886810610639124 27.965460118999502 22.689717206167852 56-57 22.530445985082075 27.298413639390706 28.019256259535425 22.151884115991795 58-59 22.87086616598082 26.56060133627432 28.137787409970862 22.430745087774 60-61 22.65192838472998 26.69064877439753 28.696086771859452 21.961336069013036 62-63 22.374478879962428 26.893996260895563 28.53469835025169 22.196826508890318 64-65 22.301307146770753 26.745728066764958 28.325737074490814 22.627227711973475 66-67 22.372201285461504 27.06516871521857 28.04803093935696 22.51459905996297 68-69 22.272307915378704 26.8249626923605 28.372668352728176 22.530061039532626 70-71 22.405242445122802 26.997995716839185 28.269021763538216 22.327740074499797 72-73 22.563262593172862 26.8866181378644 28.015246410061962 22.534872858900776 74-75 22.381664530218867 27.303225458758856 27.719672385674382 22.595437625347895 76-77 22.452526590113827 27.145397783483528 27.93318885043711 22.46888677596554 78-79 22.536123931936494 26.949556735199803 27.91471146406341 22.59960786880029 80-81 22.23820815545641 27.650382186773015 27.81792973717201 22.293479920598564 82-83 22.44469608509874 27.450277802727985 27.54686718078349 22.558158931389784 84-85 22.32437925727084 27.175061607524942 27.623267980062323 22.877291155141894 86-87 22.342047217421097 26.92001216427936 27.89758138711279 22.84035923118675 88-89 22.382658972888287 27.055833785644356 27.715181354264107 22.846325887203257 90-91 22.30468100833564 27.060312905641297 27.99634774451408 22.63865834150899 92-93 22.341608038621207 26.671063703243657 28.118236228009874 22.86909203012526 94-95 22.150504727772923 26.698668473344444 28.110456275959766 23.040370522922863 96-97 22.253830529005008 26.700946067845372 28.01806934409128 23.027154059058347 98-99 22.330799088955185 26.805119815224714 27.689827735540373 23.174253360279728 100-101 22.072194623646254 26.64579769438174 28.158963947604487 23.12304373436752 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 460.0 1 333.0 2 300.0 3 411.0 4 336.5 5 295.0 6 585.5 7 1374.5 8 2325.5 9 2774.0 10 2506.5 11 2121.5 12 2156.5 13 2383.5 14 2614.5 15 2804.0 16 2932.0 17 2992.0 18 3011.0 19 3099.0 20 3326.0 21 3751.0 22 4288.5 23 4878.5 24 5589.0 25 6804.0 26 8570.0 27 10425.0 28 11773.5 29 13350.5 30 15894.0 31 18354.0 32 21203.0 33 24223.0 34 27969.5 35 31127.0 36 34657.0 37 38547.0 38 40446.5 39 41459.5 40 43035.5 41 47496.0 42 52000.0 43 55083.0 44 57458.0 45 59922.5 46 60134.0 47 60120.5 48 63611.0 49 62975.0 50 59031.5 51 55918.5 52 53898.5 53 53265.5 54 52544.0 55 48626.0 56 41984.5 57 37395.5 58 34351.5 59 30535.0 60 26278.5 61 22100.5 62 18175.5 63 14337.0 64 10723.0 65 8358.5 66 6617.5 67 5690.0 68 4158.5 69 2381.5 70 1781.0 71 1393.5 72 1227.0 73 1040.5 74 844.5 75 719.5 76 469.0 77 265.0 78 160.0 79 113.0 80 78.0 81 55.0 82 36.5 83 21.5 84 9.0 85 3.5 86 6.0 87 5.0 88 2.5 89 1.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03945691881883307 2 0.0 3 0.0 4 0.0 5 0.009110378003698044 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00503637093866406 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.5663036630135333E-4 22-23 0.0014114670146574435 24-25 0.0 26-27 0.0011548366483560902 28-29 0.0016039397893834583 30-31 3.2078795787669166E-5 32-33 0.0 34-35 0.0 36-37 4.170243452396992E-4 38-39 0.0 40-41 2.2455157051368417E-4 42-43 0.02110784762828631 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2831518315067667E-4 84-85 3.207879578766917E-4 86-87 0.0 88-89 0.0 90-91 0.011099263342533533 92-93 0.0023417520924998493 94-95 0.0 96-97 0.0 98-99 7.6989109890406E-4 100-101 0.013344779047670374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1558662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.85009674261419 #Duplication Level Percentage of deduplicated Percentage of total 1 72.15876213510701 33.08486224721943 2 14.461278297387764 13.261020179141921 3 5.367652173137281 7.383221142571431 4 2.4483935836377 4.490363306951377 5 1.385735059162142 3.1768043261108208 6 0.8670015329182865 2.3851262496178944 7 0.5844232432505188 1.8757103569168043 8 0.434334580707846 1.593142603529401 9 0.3118409386340019 1.2868143484208945 >10 1.7235373813568728 14.768666106352937 >50 0.1554472051806317 4.956146405329994 >100 0.09498963289107348 8.002412377670765 >500 0.004918048553412732 1.4378078070616003 >1k 0.0012646410565918453 0.9178209445444979 >5k 4.2154701886394847E-4 1.3800815985601353 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8914 0.5719007713025659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7347 0.4713658253040108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5160 0.3310531725287458 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2565 0.16456422239074284 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2207 0.1415958046067717 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.11689513185026644 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1713 0.10990195436855457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.2078795787669166E-5 0.0 24-25 0.0 0.0 0.0 9.62363873630075E-5 0.0 26-27 0.0 0.0 0.0 1.92472774726015E-4 0.0 28-29 0.0 0.0 0.0 7.057335073287216E-4 0.0 30-31 0.0 0.0 0.0 0.0017001761767464658 0.0 32-33 0.0 0.0 0.0 0.00269461884616421 0.0 34-35 0.0 0.0 0.0 0.003560746332431278 0.0 36-37 0.0 0.0 0.0 0.004940134551301052 0.0 38-39 0.0 0.0 0.0 0.006864862298561201 0.0 40-41 0.0 0.0 0.0 0.010136899468903457 0.0 42-43 0.0 0.0 0.0 0.013697645801334736 0.0 44-45 0.0 0.0 0.0 0.02078705967040962 0.0 46-47 0.0 0.0 0.0 0.038494554945203 0.0 48-49 0.0 0.0 0.0 0.052545067500202096 0.0 50-51 0.0 0.0 0.0 0.06903356853506404 0.0 52-53 0.0 0.0 0.0 0.09241901066427487 0.0 54-55 0.0 0.0 0.0 0.1190123323722526 0.0 56-57 0.0 0.0 0.0 0.14746622423591516 0.0 58-59 0.0 0.0 0.0 0.1805073838972144 0.0 60-61 0.0 0.0 0.0 0.226797086218821 0.0 62-63 0.0 0.0 0.0 0.2835444759672078 0.0 64-65 0.0 0.0 0.0 0.3309248573455951 0.0 66-67 0.0 0.0 0.0 0.4024605719520974 0.0 68-69 0.0 0.0 0.0 0.4929548548691121 0.0 70-71 0.0 0.0 0.0 0.5651642241871554 0.0 72-73 0.0 0.0 0.0 0.6806478890227644 0.0 74-75 0.0 0.0 0.0 0.7790335557035457 0.0 76-77 0.0 0.0 0.0 0.8741151064181971 0.0 78-79 0.0 0.0 0.0 0.9894062984790801 0.0 80-81 0.0 0.0 0.0 1.1156363599035584 0.0 82-83 0.0 0.0 0.0 1.2639045540341651 0.0 84-85 0.0 0.0 0.0 1.46064380859994 0.0 86-87 0.0 0.0 0.0 1.7039293958536232 0.0 88-89 0.0 0.0 0.0 1.985902010827235 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5375 0.0 42.347164 1 GTATCAA 8675 0.0 36.81011 1 ATCAACG 11175 0.0 28.480082 3 TCAACGC 11310 0.0 28.140135 4 TATCAAC 11360 0.0 27.932652 2 CAACGCA 11485 0.0 27.752718 5 AACGCAG 11760 0.0 27.222298 6 GTACATG 9050 0.0 26.201084 1 TACATGG 9175 0.0 25.524359 2 ACATGGG 9225 0.0 24.201677 3 GGACCGA 280 1.8189894E-12 23.748829 6 ACGCAGA 13440 0.0 23.645082 7 CGCAGAG 13605 0.0 23.25357 8 ATGGGAG 2410 0.0 23.061249 5 GAGTACT 7615 0.0 22.675062 12-13 AGTACTT 7835 0.0 22.493078 12-13 GTACTTT 7910 0.0 21.617138 14-15 TACACCG 110 0.0028770368 21.591923 5 GCAGAGT 14520 0.0 21.526493 9 CATGGGA 5410 0.0 20.897469 4 >>END_MODULE