FastQCFastQC Report
Thu 26 May 2016
SRR937604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368147
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68220.4986306296033979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52750.3855579846317683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34280.2505578713398487No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG19690.14391728374217097No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19630.14347873437576517No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG19030.13909324071170714No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC14590.10664058759767774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.038.4079061
GTATCAA74000.037.204831
TATCAAC93950.028.941722
ATCAACG93850.028.8712523
TCAACGC94550.028.6585544
CAACGCA96150.028.0817455
AACGCAG99200.027.1544936
GTACATG84550.026.094971
TACATGG87200.024.6403582
ACGCAGA112000.024.0078247
CGCAGAG112250.023.9120358
GGACCGA2602.3646862E-1123.7541966
ACATGGG86250.023.589013
GAGTACT53800.022.42427312-13
GCAGAGT117900.022.2414849
ATGGGCG1503.0159257E-522.183595
CATGGGG35200.021.8782884
AGTACTT55550.021.41857312-13
GTACTTT56700.021.19319314-15
GTCTACG1353.7694568E-421.1334291
Produced by FastQC (version 0.11.5)