##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937604_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1368147 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.821860516450354 33.0 31.0 34.0 30.0 34.0 2 31.91592131547268 33.0 31.0 34.0 30.0 34.0 3 31.96222920490269 33.0 31.0 34.0 30.0 34.0 4 35.574466047873514 37.0 35.0 37.0 33.0 37.0 5 35.58485966785733 37.0 35.0 37.0 33.0 37.0 6 35.65936262696918 37.0 35.0 37.0 33.0 37.0 7 35.576786704937405 37.0 35.0 37.0 33.0 37.0 8 35.57486439688133 37.0 35.0 37.0 33.0 37.0 9 37.140106289748104 39.0 37.0 39.0 34.0 39.0 10-11 37.05790386559339 39.0 37.0 39.0 33.0 39.0 12-13 36.95736715426047 39.0 37.0 39.0 33.0 39.0 14-15 38.09131438361521 40.0 38.0 41.0 33.0 41.0 16-17 38.011113937318136 40.0 38.0 41.0 33.0 41.0 18-19 37.94030100566679 40.0 38.0 41.0 33.0 41.0 20-21 37.95249596717312 40.0 38.0 41.0 33.0 41.0 22-23 37.95550880132033 40.0 38.0 41.0 33.0 41.0 24-25 37.877466383363775 40.0 38.0 41.0 33.0 41.0 26-27 37.774085313931906 40.0 38.0 41.0 33.0 41.0 28-29 37.626535379604675 40.0 37.0 41.0 32.5 41.0 30-31 37.37641898129368 40.0 37.0 41.0 32.0 41.0 32-33 37.234577132428015 39.5 36.5 41.0 32.0 41.0 34-35 37.20630714389608 40.0 36.5 41.0 31.5 41.0 36-37 37.04312986835479 40.0 36.0 41.0 31.0 41.0 38-39 36.873542462907864 39.5 36.0 41.0 31.0 41.0 40-41 36.62313735293064 39.0 35.5 41.0 30.5 41.0 42-43 36.33771773062398 39.0 35.0 41.0 30.0 41.0 44-45 36.04186574980612 39.0 35.0 41.0 29.5 41.0 46-47 35.628769057710905 39.0 35.0 40.0 27.5 41.0 48-49 35.23566510031451 39.0 35.0 40.0 26.0 41.0 50-51 34.34795932016077 37.5 34.0 39.5 25.0 40.5 52-53 34.44891484613861 38.0 34.0 39.5 25.5 40.5 54-55 34.68520561021586 38.0 34.0 40.0 25.0 41.0 56-57 34.43601016557432 38.0 34.0 40.0 23.5 41.0 58-59 34.14848294810426 38.0 34.0 40.0 23.0 41.0 60-61 33.798076887936745 37.5 33.5 40.0 22.5 41.0 62-63 33.411800413259684 37.0 33.0 40.0 21.5 41.0 64-65 32.966027773331376 36.0 32.5 39.0 19.5 41.0 66-67 32.45912792996659 36.0 32.0 39.0 18.0 40.0 68-69 31.98810909938771 35.0 31.0 38.5 14.5 40.0 70-71 31.512925877117006 35.0 31.0 38.0 10.0 40.0 72-73 31.035281296527344 35.0 30.5 37.0 7.5 39.5 74-75 30.482921425840935 34.5 30.0 36.5 4.5 39.0 76-77 29.93747638228933 34.0 29.0 36.0 2.0 39.0 78-79 29.40052640542281 34.0 29.0 36.0 2.0 37.5 80-81 28.84613385842311 34.0 28.5 35.0 2.0 37.0 82-83 28.297747610454138 34.0 27.5 35.0 2.0 36.5 84-85 27.748716694916553 33.0 26.0 35.0 2.0 36.0 86-87 27.146137074451794 33.0 25.5 35.0 2.0 36.0 88-89 26.586748719253123 32.0 25.0 35.0 2.0 35.0 90-91 26.01680667355189 32.0 23.5 34.5 2.0 35.0 92-93 25.322925460495107 32.0 20.5 34.0 2.0 35.0 94-95 24.521375992492036 31.0 16.5 34.0 2.0 35.0 96-97 23.60370230684276 31.0 4.5 34.0 2.0 35.0 98-99 22.381070528240024 30.5 2.0 34.0 2.0 35.0 100-101 19.169866249752403 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11950301694113108 1101 2 0.0524565258708094 1101 3 0.13871194508479334 1101 4 0.1834336570917401 1101 5 -0.005424023410782297 1101 6 0.09312377525544235 1101 7 0.1558238772982392 1101 8 0.2555040990223745 1101 9 0.46538292682306803 1101 10-11 0.4497920549925425 1101 12-13 0.42135207778328976 1101 14-15 0.5132074494550523 1101 16-17 0.5123087807524911 1101 18-19 0.66588387766609 1101 20-21 0.5056611894541874 1101 22-23 0.4409605815850526 1101 24-25 0.5168578231993948 1101 26-27 0.6238554191823553 1101 28-29 0.5505431746057283 1101 30-31 0.5288705591153473 1101 32-33 0.46790827702299964 1101 34-35 0.47421395543889844 1101 36-37 0.5681497769815635 1101 38-39 0.6586548239197185 1101 40-41 0.5925556776937455 1101 42-43 0.5478817536250347 1101 44-45 0.7986012462931313 1101 46-47 0.8311713699710523 1101 48-49 0.7833104265061266 1101 50-51 0.6338692272481978 1101 52-53 0.8174682744033817 1101 54-55 0.8564818089826005 1101 56-57 1.0784688595365282 1101 58-59 0.9637292928055885 1101 60-61 1.1491273624830214 1101 62-63 1.006134833687284 1101 64-65 1.0325171687562573 1101 66-67 1.0339476580122309 1101 68-69 1.2585045410702058 1101 70-71 1.144680104727481 1101 72-73 1.0794444336146434 1101 74-75 1.2377470217196347 1101 76-77 1.217450411169171 1101 78-79 1.5041957474577998 1101 80-81 1.7068939526215843 1101 82-83 1.526557068173613 1101 84-85 1.7060444414771503 1101 86-87 2.00781919056692 1101 88-89 2.1736780227679695 1101 90-91 2.2892052428989267 1101 92-93 2.2463376519891654 1101 94-95 2.5812945736313075 1101 96-97 2.7021473180592217 1101 98-99 2.9797856562334637 1101 100-101 3.6256235163305632 1102 1 0.09301245653519175 1102 2 0.030460669810373986 1102 3 0.02365484191928502 1102 4 0.09924206713532158 1102 5 0.013235769159884114 1102 6 -0.04079285532937149 1102 7 0.0339958968141616 1102 8 0.03501469949146241 1102 9 0.1550351871110891 1102 10-11 0.20867969721425794 1102 12-13 0.041493929740887836 1102 14-15 0.20114520725040563 1102 16-17 0.2978661638595739 1102 18-19 0.21271410349158515 1102 20-21 0.3683890580956799 1102 22-23 0.1940194962160504 1102 24-25 0.12719278345573315 1102 26-27 0.1787349147245152 1102 28-29 0.15066194311303605 1102 30-31 0.27822946387131253 1102 32-33 0.31992352121560685 1102 34-35 0.39356777075431637 1102 36-37 0.368017245983566 1102 38-39 0.3408588063734328 1102 40-41 0.2009661234241804 1102 42-43 0.3092707732128943 1102 44-45 0.35346487922426206 1102 46-47 0.24589441995346561 1102 48-49 0.4051866650744458 1102 50-51 0.27462822860969993 1102 52-53 0.28304216356261236 1102 54-55 0.5053021445818331 1102 56-57 0.38111005129673003 1102 58-59 0.5260018821690551 1102 60-61 0.7247010863136225 1102 62-63 0.7855015535718906 1102 64-65 0.8080860220950257 1102 66-67 0.7928205547082072 1102 68-69 0.6832009284610798 1102 70-71 0.8173120068064641 1102 72-73 0.6877972377444586 1102 74-75 0.7017158827439474 1102 76-77 0.7229160860428436 1102 78-79 0.9275252692961473 1102 80-81 1.1244244613377532 1102 82-83 1.3376364987460931 1102 84-85 1.4990223987035058 1102 86-87 1.2916111320924237 1102 88-89 1.124408976352182 1102 90-91 1.2264804574128156 1102 92-93 1.577304551858557 1102 94-95 1.5516536093096676 1102 96-97 1.5862445813343307 1102 98-99 1.8614552697711026 1102 100-101 2.637647822318989 1103 1 0.022797451272825953 1103 2 -0.0475020335605123 1103 3 0.07357754950366768 1103 4 0.0523655900489004 1103 5 -0.04179084878150263 1103 6 0.1067060125190551 1103 7 0.001521828262049496 1103 8 0.012576940818455284 1103 9 0.224950836191816 1103 10-11 0.19635162444684795 1103 12-13 0.2822255922615895 1103 14-15 0.49176559030027533 1103 16-17 0.42188155211778877 1103 18-19 0.3968550682917993 1103 20-21 0.41031130291592177 1103 22-23 0.23294187698451907 1103 24-25 0.25099817222713483 1103 26-27 0.3163055110150168 1103 28-29 0.3093985970041686 1103 30-31 0.2863782542472535 1103 32-33 0.5135119870444385 1103 34-35 0.4541493590436829 1103 36-37 0.5039602899812721 1103 38-39 0.568480918936757 1103 40-41 0.6349222976791893 1103 42-43 0.6495372407289253 1103 44-45 0.7615791873693638 1103 46-47 0.733785124786472 1103 48-49 0.7370133139868074 1103 50-51 0.5592911985651483 1103 52-53 0.5889580719353091 1103 54-55 0.4667443931474011 1103 56-57 0.5729320905740565 1103 58-59 0.5870835978536135 1103 60-61 0.7251489951868919 1103 62-63 0.7721183065341677 1103 64-65 0.7778303404047335 1103 66-67 0.970447483980017 1103 68-69 1.0587169239846865 1103 70-71 1.2690458868137036 1103 72-73 1.1333108114090358 1103 74-75 1.0620367385515017 1103 76-77 1.2100869855609027 1103 78-79 1.2838493824590884 1103 80-81 1.5087723645390163 1103 82-83 1.6028872331815975 1103 84-85 1.6763805826513511 1103 86-87 1.6389842703445616 1103 88-89 1.6234202006334364 1103 90-91 1.7782362151800974 1103 92-93 1.7817679750347573 1103 94-95 2.09838108354478 1103 96-97 2.0657042456772707 1103 98-99 2.0480176434450463 1103 100-101 3.0721826768318827 1104 1 0.19557655909929395 1104 2 0.1038366003949669 1104 3 0.02159978044460331 1104 4 0.09921844573906213 1104 5 -0.0062007030633211 1104 6 0.17103988943750892 1104 7 0.13143021949031208 1104 8 0.19498666876789628 1104 9 0.4724043934715425 1104 10-11 0.18401108571929825 1104 12-13 0.21375099352259497 1104 14-15 0.27267276357760295 1104 16-17 0.3571086256814837 1104 18-19 0.4682858003345842 1104 20-21 0.38185915931335757 1104 22-23 0.33295464367262184 1104 24-25 0.29245988239394904 1104 26-27 0.351688809695915 1104 28-29 0.28484918992119646 1104 30-31 0.21038487691110674 1104 32-33 0.21805034449212712 1104 34-35 0.4413991297326092 1104 36-37 0.23690274913899145 1104 38-39 0.30908605995306004 1104 40-41 0.44021740390070363 1104 42-43 0.4368066285239749 1104 44-45 0.42050043336253395 1104 46-47 0.24746327435511972 1104 48-49 0.18317703541856645 1104 50-51 0.13194121604705344 1104 52-53 0.1306664416336929 1104 54-55 0.37262076178529213 1104 56-57 0.4903609774522977 1104 58-59 0.5055280369600652 1104 60-61 0.35509119367286957 1104 62-63 0.4637013669628587 1104 64-65 0.46394196307304014 1104 66-67 0.5625355625623172 1104 68-69 0.5034184815206366 1104 70-71 0.6789575520079758 1104 72-73 0.5167665181186258 1104 74-75 0.4426895566978146 1104 76-77 0.5936539003550472 1104 78-79 0.8495726931860403 1104 80-81 1.0233937810809088 1104 82-83 1.0267297127126405 1104 84-85 1.41897145522557 1104 86-87 1.519289896850875 1104 88-89 1.3948838057860193 1104 90-91 1.4317425171198295 1104 92-93 1.6860476179945287 1104 94-95 1.8585270419316053 1104 96-97 2.1440343272862137 1104 98-99 2.4259574291604125 1104 100-101 2.5888676705908864 1105 1 0.11006416170570787 1105 2 0.054552043875069955 1105 3 0.14718900885180375 1105 4 0.141329038877835 1105 5 0.03814916725760753 1105 6 0.12852245088046743 1105 7 0.09015703046388523 1105 8 0.1544062793680112 1105 9 0.22866586070026074 1105 10-11 0.24853374677660867 1105 12-13 0.17269122397843972 1105 14-15 0.23422261678777545 1105 16-17 0.22338899843558835 1105 18-19 0.2341079024095407 1105 20-21 0.2957450956669305 1105 22-23 0.20977417262466957 1105 24-25 0.1669129659102495 1105 26-27 0.06966310078520621 1105 28-29 0.11088929604508024 1105 30-31 0.18405430640689247 1105 32-33 0.21505828704462715 1105 34-35 0.27312995708415855 1105 36-37 0.29748598413197413 1105 38-39 0.30512664034245773 1105 40-41 0.19229464036484245 1105 42-43 0.17952060829050964 1105 44-45 0.24376678366620297 1105 46-47 0.3299006178358681 1105 48-49 0.42700112196923357 1105 50-51 0.4900493430051398 1105 52-53 0.496048964670436 1105 54-55 0.5066751646896606 1105 56-57 0.6458951910596369 1105 58-59 0.8291224209124053 1105 60-61 0.7833692285769516 1105 62-63 0.8598874352738335 1105 64-65 0.887311117401822 1105 66-67 0.8664669912081422 1105 68-69 0.8728508600923952 1105 70-71 0.8681883305380715 1105 72-73 0.9265312266266683 1105 74-75 0.9377929977266177 1105 76-77 1.024173072306052 1105 78-79 1.3317709975473306 1105 80-81 1.3125402147437164 1105 82-83 1.4355321736857007 1105 84-85 1.7148371153334239 1105 86-87 1.63368064699943 1105 88-89 1.5112388056139814 1105 90-91 1.6022811897529436 1105 92-93 1.899639137755127 1105 94-95 1.8644718671833616 1105 96-97 1.8224562692268158 1105 98-99 2.1382668046457205 1105 100-101 2.8175087510979218 1106 1 0.03478050195287352 1106 2 -0.07965437989556534 1106 3 0.06273531843583768 1106 4 -8.074688292438736E-4 1106 5 -0.05753218398492521 1106 6 -0.01610840428013205 1106 7 0.18714750114807543 1106 8 0.2867904666918548 1106 9 0.2017679988598573 1106 10-11 -0.10671821741876641 1106 12-13 -0.2086641482636864 1106 14-15 -0.339528918949739 1106 16-17 -0.07611337098800419 1106 18-19 0.16768712581676226 1106 20-21 0.14469046987067458 1106 22-23 0.0258480532139842 1106 24-25 -0.10437158173116501 1106 26-27 -0.0888183000138909 1106 28-29 0.16053052711291826 1106 30-31 0.3309255315898909 1106 32-33 0.3913237863787131 1106 34-35 0.3716923002840602 1106 36-37 0.20559003228444084 1106 38-39 -0.022729831436109293 1106 40-41 0.2221194372345181 1106 42-43 0.2414033450521913 1106 44-45 0.41727982840133393 1106 46-47 0.4172704027809999 1106 48-49 0.5807338675065594 1106 50-51 0.7376892112672593 1106 52-53 0.5941549744372168 1106 54-55 0.6614057524512518 1106 56-57 0.43986116221357463 1106 58-59 0.2742669092924288 1106 60-61 0.4850820275940748 1106 62-63 0.3256242279312005 1106 64-65 0.6587727265450098 1106 66-67 0.6819544523630157 1106 68-69 0.7034252001124166 1106 70-71 0.6354035609245052 1106 72-73 0.6237792882420052 1106 74-75 0.8790359384961874 1106 76-77 1.2161170320826358 1106 78-79 1.003833480125099 1106 80-81 1.1569589058307628 1106 82-83 1.0649181884081322 1106 84-85 1.0444094834148636 1106 86-87 1.1968090088449976 1106 88-89 1.4446172216620532 1106 90-91 1.88288443777326 1106 92-93 1.8921277036085833 1106 94-95 1.8867165734058098 1106 96-97 1.8897133129708124 1106 98-99 1.9691870082406382 1106 100-101 2.2248085373852042 1107 1 0.1262288308041981 1107 2 0.11751042993924088 1107 3 0.05818928181143335 1107 4 0.0495267609056782 1107 5 0.11974275518782918 1107 6 0.1801267090506471 1107 7 0.0731610752292653 1107 8 0.12644727294104996 1107 9 0.07144657486190198 1107 10-11 0.17225220132883834 1107 12-13 0.17891172572786473 1107 14-15 0.3094927227457305 1107 16-17 0.12291851529705156 1107 18-19 0.287002018888856 1107 20-21 0.2527529618670812 1107 22-23 0.1985423802725066 1107 24-25 0.372379602799505 1107 26-27 0.27643845501236086 1107 28-29 0.3089579826129878 1107 30-31 0.30699929263484194 1107 32-33 0.35427166702923785 1107 34-35 0.4305807623150528 1107 36-37 0.36053816904971114 1107 38-39 0.5098044239102819 1107 40-41 0.44126657515230505 1107 42-43 0.4264014241916456 1107 44-45 0.1990803150193443 1107 46-47 0.6179363011034482 1107 48-49 0.5325074620769286 1107 50-51 0.2790376553702174 1107 52-53 0.282556818463334 1107 54-55 0.2837837778066117 1107 56-57 0.5223454751857872 1107 58-59 0.4534473421263314 1107 60-61 0.5852382575851109 1107 62-63 0.7858419254596924 1107 64-65 0.6329422745024971 1107 66-67 0.8880077242288138 1107 68-69 0.7830461502981017 1107 70-71 0.9202787049890411 1107 72-73 0.8953505521682423 1107 74-75 0.9711258347697189 1107 76-77 1.0083900503948406 1107 78-79 1.188310365769869 1107 80-81 1.239671160921045 1107 82-83 1.327942903480487 1107 84-85 1.5065890198471834 1107 86-87 1.471051549503212 1107 88-89 1.3481285547392723 1107 90-91 1.8593116371585872 1107 92-93 2.0996024122780703 1107 94-95 2.093335858845684 1107 96-97 2.3525190499409767 1107 98-99 2.534694755711506 1107 100-101 3.0511051182534317 1108 1 0.004404861598484189 1108 2 0.06054396313932742 1108 3 0.12918639484718852 1108 4 0.0641940197701345 1108 5 0.06775963282495923 1108 6 0.10098687634651782 1108 7 0.14905270303295026 1108 8 0.10960812866686354 1108 9 0.19565858167217698 1108 10-11 0.2514675392516992 1108 12-13 0.13061947611649316 1108 14-15 0.22447828792450508 1108 16-17 0.3207318249424631 1108 18-19 0.4143593362328488 1108 20-21 0.3508460849702004 1108 22-23 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-1.8879676943078962 2316 74-75 -2.086022318167906 2316 76-77 -2.0425586973960144 2316 78-79 -2.035866538407035 2316 80-81 -2.191606049303534 2316 82-83 -2.066869803963801 2316 84-85 -2.2308160407157693 2316 86-87 -2.0431068193919977 2316 88-89 -2.1623598283806906 2316 90-91 -2.4519440204128635 2316 92-93 -2.732473108218503 2316 94-95 -2.9204897370851732 2316 96-97 -2.8575762127858653 2316 98-99 -3.1991687728888643 2316 100-101 -3.3285965930272248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4826.0 3 940.0 4 878.0 5 1044.0 6 1252.0 7 1413.0 8 1808.0 9 2360.0 10 2896.0 11 3719.0 12 5029.0 13 6469.0 14 7627.0 15 9628.0 16 12460.0 17 16370.0 18 14267.0 19 9598.0 20 9786.0 21 10357.0 22 11436.0 23 12739.0 24 14217.0 25 16015.0 26 18801.0 27 22057.0 28 26510.0 29 31585.0 30 38417.0 31 47935.0 32 60361.0 33 78865.0 34 106067.0 35 146959.0 36 203182.0 37 249797.0 38 150326.0 39 10151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40835062659031 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22.094280177698096 26.985188895773003 29.4950719976096 21.425458928919298 20-21 22.94171107682145 26.091493828959695 29.468630697675778 21.498164396543075 22-23 22.576681904355944 25.938751153995877 29.51420664591763 21.970360295730554 24-25 22.339083462997188 26.279131339445634 29.64084607359292 21.74093912396426 26-27 22.006090870871486 26.66649332538405 29.522962348808555 21.804453454935903 28-29 21.72910527730595 26.656551384680295 29.891463976485085 21.722879361528673 30-31 21.73194092647746 26.290823270197933 29.87817294577993 22.099062857544677 32-33 21.766071308235695 26.477811764774824 29.626882835687518 22.129234091301967 34-35 22.37314119944024 26.539073861994535 29.20496459017666 21.882820348388567 36-37 22.48494859948096 26.14712343485026 29.280334142739385 22.087593822929396 38-39 21.917985281700403 26.470823010579924 29.480319089337254 22.130872618382423 40-41 22.01136157678035 26.497591660609963 29.441349475584328 22.049697287025356 42-43 22.272783364647715 26.323379009244913 29.004649371069757 22.39918825503761 44-45 22.056460590223868 26.703873014571045 28.818783285778732 22.420883109426356 46-47 22.245801737494933 26.98251335823281 28.425982518118893 22.34570238615336 48-49 22.529851273763 26.866604186191818 28.27124290365005 22.332301636395133 50-51 22.49815696943884 27.102565520514677 27.93484992256159 22.4644275874849 52-53 22.71496441143625 27.242603281553833 27.714944266620662 22.327488040389255 54-55 22.296742288216873 27.10803509336025 27.851042550534505 22.744180067888376 56-57 22.40876893206443 27.603880819205944 27.74209445464927 22.245255794080357 58-59 22.527634644304168 26.889032388432767 27.962167370253717 22.62116559700935 60-61 22.45913199611018 27.050649670185468 28.416004206160444 22.074214127543907 62-63 22.286112193164612 27.206242000533052 28.2473442501955 22.260301556106835 64-65 22.234158118404824 27.06643863979113 28.063928074143153 22.6354751676609 66-67 22.283265152793852 27.364311084863264 27.75179232499362 22.600631437349268 68-69 22.066460465095844 27.22849272216828 28.07072655299219 22.634320259743685 70-71 22.247176283601483 27.30579249505994 28.02180400777522 22.42522721356336 72-73 22.323960371676645 27.206207597576835 27.851508032293214 22.6183239984533 74-75 22.07235034060811 27.69634813470233 27.599125693785563 22.632175830903993 76-77 22.28182837164015 27.470871207141705 27.58310572125048 22.664194699967666 78-79 22.460670026613 27.336400346247302 27.597953274190072 22.60497635294962 80-81 22.159644526465534 27.970948423748766 27.5314765077325 22.3379305420532 82-83 22.399443767704916 27.731577642771597 27.389131575372733 22.479847014150753 84-85 22.314563655607785 27.350059987354513 27.422623268796436 22.91275308824127 86-87 22.44834521247909 27.181843378341913 27.626986402231644 22.74282500694735 88-89 22.43802326198754 27.259658777802798 27.4803419178965 22.821976042313164 90-91 22.270939980982376 27.363518165052025 27.743321405517307 22.622220448448292 92-93 22.384342080019216 27.001908287944982 27.775914564938176 22.837835067097632 94-95 22.181613276806267 27.061045350412034 27.75861444003165 22.99872693275005 96-97 22.314356435643564 27.080123322983184 27.70010691897592 22.90541332239733 98-99 22.334210533060862 27.287160674014544 27.24428321968306 23.13434557324154 100-101 22.47663481261905 26.466573019207978 28.02962159177982 23.027170576393154 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1976.0 1 1151.5 2 343.5 3 363.0 4 342.5 5 332.5 6 524.0 7 1054.0 8 1746.5 9 2139.5 10 1952.5 11 1702.5 12 1813.0 13 1990.0 14 2098.0 15 2183.5 16 2258.0 17 2375.0 18 2465.5 19 2626.0 20 2836.0 21 3098.0 22 3515.5 23 4116.0 24 4966.5 25 6023.5 26 7637.5 27 9463.5 28 10718.5 29 12216.0 30 14372.5 31 16613.5 32 19294.0 33 22032.0 34 25307.5 35 28086.0 36 31124.5 37 34429.0 38 35991.0 39 36916.0 40 38319.5 41 42143.0 42 45858.0 43 48083.0 44 50514.0 45 52828.0 46 52688.0 47 52585.5 48 55246.5 49 54831.0 50 51467.5 51 48611.0 52 46867.0 53 46299.5 54 45522.5 55 42183.5 56 36839.5 57 32374.5 58 29356.0 59 26272.0 60 22614.0 61 19077.5 62 15713.0 63 12269.5 64 9416.5 65 7518.0 66 5768.0 67 4672.5 68 3487.0 69 2237.0 70 1699.0 71 1330.5 72 1067.0 73 901.5 74 703.5 75 554.0 76 365.5 77 207.5 78 140.5 79 96.5 80 61.0 81 35.5 82 23.0 83 18.0 84 14.0 85 10.0 86 9.0 87 8.0 88 7.0 89 6.0 90 5.0 91 4.5 92 3.0 93 3.0 94 3.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32284542523573856 2 0.27738247425167034 3 0.2831567075760134 4 0.277236291129535 5 0.2776748404959409 6 0.2110153368022588 7 0.2020250747909399 8 0.2099189633862443 9 0.21094224524119118 10-11 0.2130619005121526 12-13 0.21733775683460912 14-15 0.2116366150713337 16-17 0.20180580010773697 18-19 0.19625084146659677 20-21 0.1941311861956354 22-23 0.2033772686706911 24-25 0.20154997964400026 26-27 0.20648366001606552 28-29 0.21094224524119118 30-31 0.21437754861136998 32-33 0.21335426675642308 34-35 0.2133177209758893 36-37 0.20045360622798575 38-39 0.19266935497428272 40-41 0.1885396817739614 42-43 0.19687211973567165 44-45 0.19727412332154365 46-47 0.20396200115923216 48-49 0.21346390409802454 50-51 0.2096631429225076 52-53 0.22157706737653188 54-55 0.20593547330805825 56-57 0.18327708937709178 58-59 0.1673065832838138 60-61 0.18499474106218117 62-63 0.1776855849554178 64-65 0.18408109654883575 66-67 0.18510437840378263 68-69 0.1822172617416111 70-71 0.18466582903737683 72-73 0.1943504608788383 74-75 0.19891868344556543 76-77 0.19752994378528038 78-79 0.19361954526816197 80-81 0.19329063324335763 82-83 0.18495819528164736 84-85 0.1785261379076956 86-87 0.18408109654883575 88-89 0.1910978864113286 90-91 0.18795494928542036 92-93 0.1845561916957754 94-95 0.18620075181979714 96-97 0.19201153092467402 98-99 0.19204807670520785 100-101 0.1744330104879081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1368147.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.4443236130629 #Duplication Level Percentage of deduplicated Percentage of total 1 74.78444434700471 36.97666267516393 2 13.530269320542695 13.37990029713619 3 4.669910432468545 6.927016880009797 4 2.2093034248157664 4.369500539841558 5 1.196692438403651 2.9584824094867725 6 0.7581083742274148 2.249049347344397 7 0.4886724627437374 1.691345557208564 8 0.3394962477438251 1.342892987109301 9 0.26620146858568744 1.1845936403120991 >10 1.532124127887899 14.169098401918143 >50 0.13875092892110877 4.744047462201779 >100 0.08187344145745912 7.179856740457001 >500 0.002669776198420538 0.8855354087914342 >1k 0.001186567199298017 1.0548688463814484 >5k 2.9664179982450426E-4 0.8871488066376795 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6822 0.4986306296033979 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5275 0.3855579846317683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3428 0.2505578713398487 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1969 0.14391728374217097 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1963 0.14347873437576517 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1903 0.13909324071170714 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1459 0.10664058759767774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 7.309156106763381E-5 0.0 0.0 0.0 12-13 0.0 7.309156106763381E-5 0.0 0.0 0.0 14-15 0.0 7.309156106763381E-5 0.0 0.0 0.0 16-17 0.0 7.309156106763381E-5 0.0 0.0 0.0 18-19 0.0 7.309156106763381E-5 0.0 0.0 0.0 20-21 0.0 7.309156106763381E-5 0.0 0.0 0.0 22-23 0.0 7.309156106763381E-5 0.0 1.096373416014507E-4 0.0 24-25 0.0 7.309156106763381E-5 0.0 2.192746832029014E-4 0.0 26-27 0.0 7.309156106763381E-5 0.0 2.9236624427053524E-4 0.0 28-29 0.0 7.309156106763381E-5 0.0 8.040071717439719E-4 0.0 30-31 0.0 7.309156106763381E-5 0.0 0.0016080143434879439 0.0 32-33 0.0 7.309156106763381E-5 0.0 0.002521658856833367 0.0 34-35 0.0 7.309156106763381E-5 0.0 0.0030332997843068032 0.0 36-37 0.0 7.309156106763381E-5 0.0 0.004056581639253677 0.0 38-39 0.0 7.309156106763381E-5 0.0 0.0053356839579372685 0.0 40-41 0.0 7.309156106763381E-5 0.0 0.008113163278507352 0.0 42-43 0.0 7.309156106763381E-5 0.0 0.011256100404415607 0.0 44-45 0.0 7.309156106763381E-5 0.0 0.017834340900502652 0.0 46-47 0.0 7.309156106763381E-5 0.0 0.031137005014812008 0.0 48-49 0.0 7.309156106763381E-5 0.0 0.040273450148266235 0.0 50-51 0.0 7.309156106763381E-5 0.0 0.05569576953353697 0.0 52-53 0.0 7.309156106763381E-5 0.0 0.075649765705001 0.0 54-55 0.0 7.309156106763381E-5 0.0 0.09911215680771145 0.0 56-57 0.0 7.309156106763381E-5 0.0 0.1240729249123084 0.0 58-59 0.0 7.309156106763381E-5 0.0 0.1567083069290069 0.0 60-61 0.0 7.309156106763381E-5 0.0 0.20326763132908965 0.0 62-63 0.0 7.309156106763381E-5 0.0 0.25735538651913864 0.0 64-65 0.0 7.309156106763381E-5 0.0 0.3035127073333494 0.0 66-67 0.0 7.309156106763381E-5 0.0 0.3794548392826209 0.0 68-69 0.0 7.309156106763381E-5 0.0 0.4749489638174845 0.0 70-71 0.0 7.309156106763381E-5 0.0 0.5451899540034806 0.0 72-73 0.0 7.309156106763381E-5 0.0 0.6635617371525135 0.0 74-75 0.0 7.309156106763381E-5 0.0 0.7701292331891236 0.0 76-77 0.0 7.309156106763381E-5 0.0 0.8686201117277603 0.0 78-79 0.0 7.309156106763381E-5 0.0 0.9890384585866869 0.0 80-81 0.0 7.309156106763381E-5 0.0 1.1161446832833022 0.0 82-83 0.0 7.309156106763381E-5 0.0 1.2700024193306714 0.0 84-85 0.0 7.309156106763381E-5 0.0 1.4701636593143865 0.0 86-87 0.0 7.309156106763381E-5 0.0 1.728761602371675 0.0 88-89 0.0 7.309156106763381E-5 0.0 2.030958661605807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4655 0.0 38.407906 1 GTATCAA 7400 0.0 37.20483 1 TATCAAC 9395 0.0 28.94172 2 ATCAACG 9385 0.0 28.871252 3 TCAACGC 9455 0.0 28.658554 4 CAACGCA 9615 0.0 28.081745 5 AACGCAG 9920 0.0 27.154493 6 GTACATG 8455 0.0 26.09497 1 TACATGG 8720 0.0 24.640358 2 ACGCAGA 11200 0.0 24.007824 7 CGCAGAG 11225 0.0 23.912035 8 GGACCGA 260 2.3646862E-11 23.754196 6 ACATGGG 8625 0.0 23.58901 3 GAGTACT 5380 0.0 22.424273 12-13 GCAGAGT 11790 0.0 22.241484 9 ATGGGCG 150 3.0159257E-5 22.18359 5 CATGGGG 3520 0.0 21.878288 4 AGTACTT 5555 0.0 21.418573 12-13 GTACTTT 5670 0.0 21.193193 14-15 GTCTACG 135 3.7694568E-4 21.133429 1 >>END_MODULE