FastQCFastQC Report
Thu 26 May 2016
SRR937604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368147
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81710.5972311454836359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67410.49271021315691954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46470.33965648428129436No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG24240.17717394402794437No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18910.13821614197889553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.12462111162031565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49650.041.4414751
GTATCAA80500.036.834541
GGTCGAC550.002578920234.5478487
GTACCGT953.492288E-529.9987896
CCGCTAA650.00584247929.2327949
ATCAACG103400.028.6224693
CGTAAGC1004.950216E-528.5030163
TCAACGC104200.028.3571284
TATCAAC104650.028.2805842
CAACGCA106400.027.8154435
AACGCAG108300.027.3234586
GTACATG79550.024.7898851
ACGCAGA122800.023.9837467
CGCAGAG123100.023.8481188
TACATGG82500.023.7813052
GGACCGA3450.023.4048526
ACATGGG82200.022.7122573
GAGTACT69500.022.65804512-13
GCAGAGT130500.022.2774059
AGTACTT71100.022.24837512-13
Produced by FastQC (version 0.11.5)