##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1368147 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31984209299147 33.0 31.0 34.0 31.0 34.0 2 32.536618506637076 34.0 31.0 34.0 31.0 34.0 3 32.642749646054114 34.0 31.0 34.0 31.0 34.0 4 36.23282001129996 37.0 35.0 37.0 35.0 37.0 5 36.087345877307044 37.0 35.0 37.0 35.0 37.0 6 36.18727154318944 37.0 36.0 37.0 35.0 37.0 7 36.15248800019296 37.0 36.0 37.0 35.0 37.0 8 36.147674189981046 37.0 36.0 37.0 35.0 37.0 9 37.82996125416348 39.0 38.0 39.0 35.0 39.0 10-11 37.78539586754932 39.0 37.5 39.0 35.0 39.0 12-13 37.70806755414441 39.0 37.0 39.0 35.0 39.0 14-15 38.97307599256513 40.0 38.0 41.0 36.0 41.0 16-17 38.9486546401812 40.0 38.0 41.0 36.0 41.0 18-19 38.94360693697388 40.0 38.0 41.0 35.5 41.0 20-21 38.86852947819203 40.0 38.0 41.0 35.0 41.0 22-23 38.725881429407806 40.0 38.0 41.0 35.0 41.0 24-25 38.66842488416815 40.0 38.0 41.0 34.5 41.0 26-27 38.60016065525123 40.0 38.0 41.0 34.0 41.0 28-29 38.48218685565221 40.0 38.0 41.0 34.0 41.0 30-31 38.536355011559436 40.0 38.0 41.0 35.0 41.0 32-33 38.45326342856433 40.0 38.0 41.0 34.5 41.0 34-35 38.3120735564234 40.0 38.0 41.0 34.0 41.0 36-37 38.15324084327196 40.0 38.0 41.0 34.0 41.0 38-39 38.01221579260123 40.0 37.5 41.0 34.0 41.0 40-41 37.82464640130044 40.0 37.0 41.0 33.0 41.0 42-43 37.59725197657854 40.0 37.0 41.0 33.0 41.0 44-45 37.266410700019804 40.0 37.0 41.0 32.5 41.0 46-47 36.86393750086796 40.0 36.5 41.0 31.5 41.0 48-49 36.49284762529173 40.0 36.0 41.0 30.5 41.0 50-51 36.22981887180252 39.0 36.0 41.0 30.0 41.0 52-53 35.99900303110704 39.0 35.0 41.0 29.5 41.0 54-55 35.77861077793541 39.0 35.0 40.0 29.0 41.0 56-57 35.50682053902103 38.5 35.0 40.0 28.0 41.0 58-59 35.254094041064306 38.0 35.0 40.0 28.0 41.0 60-61 34.91518674528395 38.0 34.0 40.0 27.0 41.0 62-63 34.56303745138497 37.5 34.0 40.0 26.5 41.0 64-65 34.17240508512609 37.0 34.0 39.5 26.0 41.0 66-67 33.75008460348194 36.0 33.5 39.0 26.0 40.5 68-69 33.31361176832606 36.0 33.0 39.0 25.0 40.0 70-71 32.909492547218974 35.0 33.0 38.5 24.0 40.0 72-73 32.48219781938637 35.0 32.0 37.5 23.5 39.5 74-75 32.00453752411108 35.0 32.0 37.0 22.0 39.0 76-77 30.775440066016298 34.0 30.5 35.5 20.5 38.0 78-79 31.33591748547488 34.5 31.0 36.0 21.0 38.0 80-81 31.139806979805535 34.5 31.0 36.0 20.0 37.0 82-83 30.809645454764727 34.0 31.0 35.0 18.5 37.0 84-85 30.44306277030173 34.0 31.0 35.0 17.5 36.5 86-87 30.039220931668893 34.0 30.5 35.0 13.5 36.0 88-89 29.640174995815507 34.0 30.0 35.0 7.0 36.0 90-91 29.17087747149977 34.0 29.0 35.0 2.0 35.0 92-93 28.76514292689309 34.0 29.0 35.0 2.0 35.0 94-95 28.336566903994964 34.0 29.0 35.0 2.0 35.0 96-97 27.84141177812033 33.0 28.5 35.0 2.0 35.0 98-99 27.081115552641638 33.0 26.5 35.0 2.0 35.0 100-101 24.66235170635904 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19174865761250004 1101 2 0.1556937739751234 1101 3 0.10336970409521484 1101 4 0.046469223299382634 1101 5 0.02997788847268623 1101 6 0.09290786155679598 1101 7 -0.031032172857592855 1101 8 0.015990498633364325 1101 9 0.1596666813693588 1101 10-11 0.16228206263734535 1101 12-13 0.24319474030318133 1101 14-15 0.20085835008777764 1101 16-17 0.04805064561499961 1101 18-19 0.1384584602652552 1101 20-21 0.2527392852198673 1101 22-23 0.11442105575703465 1101 24-25 0.21158388112469595 1101 26-27 0.10750901837280935 1101 28-29 0.22971596415116124 1101 30-31 0.2559446068822808 1101 32-33 0.27817301040623477 1101 34-35 0.2020259016127639 1101 36-37 0.318220780755901 1101 38-39 0.4246353966540113 1101 40-41 0.2879077678682691 1101 42-43 0.16670658901740865 1101 44-45 0.3513009468392454 1101 46-47 0.2019560123616131 1101 48-49 0.24782685220738188 1101 50-51 0.23767755130923973 1101 52-53 0.25208731546739216 1101 54-55 0.2936867325523522 1101 56-57 0.3377503440884979 1101 58-59 0.22962647899875321 1101 60-61 0.17947188490372668 1101 62-63 0.3337422400290322 1101 64-65 0.2881111089282342 1101 66-67 0.3497114508890533 1101 68-69 0.37029347566479487 1101 70-71 0.11950202368299045 1101 72-73 0.20587171035848684 1101 74-75 0.1504808696645128 1101 76-77 -0.12337020417339772 1101 78-79 0.06162866745999551 1101 80-81 0.23503162779438824 1101 82-83 0.0969023534181801 1101 84-85 0.2781770891717201 1101 86-87 0.2863775481876196 1101 88-89 0.4058377833036815 1101 90-91 0.5386191749623492 1101 92-93 0.6653184157077305 1101 94-95 0.5851362742837978 1101 96-97 0.4931239190548773 1101 98-99 0.8693082331279243 1101 100-101 1.6714562218866753 1102 1 0.125149373530121 1102 2 0.20262118622025582 1102 3 0.11854463741639165 1102 4 0.10381591339803009 1102 5 -0.027545301100701636 1102 6 0.049795072136760155 1102 7 0.04714190209525526 1102 8 0.14362687575599153 1102 9 0.09922119669509755 1102 10-11 0.12595020430341464 1102 12-13 0.0800413068521948 1102 14-15 0.12992633902594974 1102 16-17 0.1424552994816608 1102 18-19 0.1395118716873185 1102 20-21 0.15093371793492594 1102 22-23 0.16058125382966182 1102 24-25 0.13688975024609817 1102 26-27 0.041703758509726185 1102 28-29 -0.17341151717452163 1102 30-31 0.04010831439003937 1102 32-33 0.017418048425120958 1102 34-35 0.10271456234816156 1102 36-37 0.13374531708731752 1102 38-39 0.1434556473547559 1102 40-41 0.11787912133326728 1102 42-43 0.14330767384127086 1102 44-45 0.20017807505427498 1102 46-47 0.2253439801019823 1102 48-49 0.1519020686873418 1102 50-51 0.13596336160046008 1102 52-53 0.14004433181303 1102 54-55 0.21952790894145124 1102 56-57 0.3939989393537573 1102 58-59 0.40369093124492395 1102 60-61 0.3756126233507189 1102 62-63 0.26538645104733405 1102 64-65 0.21790229887875512 1102 66-67 0.2292898807765269 1102 68-69 0.3254022864738886 1102 70-71 0.31331185197804245 1102 72-73 0.2475284908203932 1102 74-75 0.0566436951217284 1102 76-77 -0.013056691954496102 1102 78-79 0.29225695643834015 1102 80-81 0.3036526128223187 1102 82-83 0.33333926245213874 1102 84-85 0.4105677812596724 1102 86-87 0.5844558934000368 1102 88-89 0.7372803380866628 1102 90-91 0.7627793167338801 1102 92-93 0.9049298576995035 1102 94-95 0.7368346684066758 1102 96-97 0.8829947259077038 1102 98-99 0.9952703228280875 1102 100-101 1.6668703633820598 1103 1 0.24423686386293753 1103 2 0.19117362577056696 1103 3 0.11352400659864514 1103 4 0.1303609289150316 1103 5 0.038871707012070544 1103 6 0.04553611148341474 1103 7 0.04148609598082231 1103 8 0.10577339912783401 1103 9 0.051332310507170575 1103 10-11 0.13339044335621253 1103 12-13 0.1416296772471739 1103 14-15 0.1965677517350386 1103 16-17 0.14188922369458368 1103 18-19 0.2894870678894108 1103 20-21 0.18994455507885277 1103 22-23 0.17675594099119962 1103 24-25 0.14097292354766466 1103 26-27 0.16656364631193554 1103 28-29 0.05872749753400086 1103 30-31 0.0905628856264542 1103 32-33 0.2052975892982687 1103 34-35 0.24317293293220388 1103 36-37 0.14056433579325045 1103 38-39 0.07613089226412484 1103 40-41 0.011403979600494552 1103 42-43 0.12272119681787075 1103 44-45 0.05048490388646343 1103 46-47 0.33847383226054006 1103 48-49 0.3669755302441615 1103 50-51 0.3784875599336033 1103 52-53 0.24821657550787535 1103 54-55 0.22424288134217107 1103 56-57 0.25832554099935834 1103 58-59 0.28857159841757607 1103 60-61 0.1101554907057718 1103 62-63 0.1999285081149722 1103 64-65 0.2331991746806068 1103 66-67 0.31106993816288053 1103 68-69 0.2990188782790071 1103 70-71 0.33585337245535385 1103 72-73 0.36384524837429666 1103 74-75 0.2412845622372899 1103 76-77 0.2747636979378001 1103 78-79 0.3747700988499538 1103 80-81 0.36793271297383967 1103 82-83 0.290503193498477 1103 84-85 0.5262045726295739 1103 86-87 0.6173929511744269 1103 88-89 0.6866110406868735 1103 90-91 0.6067112093762006 1103 92-93 0.8833255696585347 1103 94-95 0.8780499824785792 1103 96-97 1.0605541312287698 1103 98-99 1.1656289740416703 1103 100-101 2.1352981897320653 1104 1 0.12716900291041355 1104 2 0.19340490477919303 1104 3 0.10251752005348891 1104 4 0.06244303784735905 1104 5 -0.005742752352048797 1104 6 0.010276476192551343 1104 7 0.08110553301881396 1104 8 0.05153098863477368 1104 9 0.1888210896114373 1104 10-11 0.12614901772194997 1104 12-13 0.14246084647118806 1104 14-15 0.09676583557525475 1104 16-17 0.02233853104614525 1104 18-19 -0.021367552895469544 1104 20-21 0.0443500411071156 1104 22-23 0.11931073766278644 1104 24-25 -0.09152915159519637 1104 26-27 -0.03821136193970176 1104 28-29 0.03699410156827554 1104 30-31 0.13173767893511013 1104 32-33 0.2767514526226478 1104 34-35 0.18232654154558503 1104 36-37 0.2223472060116407 1104 38-39 0.1617622294568264 1104 40-41 0.11637128887196724 1104 42-43 0.33091273984339153 1104 44-45 0.3417017338510746 1104 46-47 0.3815778634201621 1104 48-49 0.37661437976538537 1104 50-51 0.4855856161052543 1104 52-53 0.35949694532368426 1104 54-55 0.11515086421434262 1104 56-57 0.2519201777448288 1104 58-59 0.26779703855692816 1104 60-61 0.28722923234180797 1104 62-63 0.3765881108441178 1104 64-65 0.292630554399274 1104 66-67 0.43312481445946105 1104 68-69 0.4611131133408719 1104 70-71 0.5200128484072692 1104 72-73 0.5235445061270596 1104 74-75 0.6955079783895144 1104 76-77 0.3403115223073172 1104 78-79 0.5273607134214195 1104 80-81 0.6436629274986849 1104 82-83 0.7270173894328913 1104 84-85 0.7383284335251616 1104 86-87 0.7942847563841262 1104 88-89 0.8729675435573725 1104 90-91 0.8767053108993963 1104 92-93 1.0826355902186826 1104 94-95 1.2179946364390517 1104 96-97 1.5475630252065393 1104 98-99 1.782864759201889 1104 100-101 2.466724304415184 1105 1 0.08497983048513902 1105 2 0.08760463751662684 1105 3 0.009027697698471115 1105 4 0.0898918520440759 1105 5 -0.01612730729105749 1105 6 -0.006562274461892059 1105 7 -0.019674959231601008 1105 8 0.0072104111839550455 1105 9 0.03816320894593872 1105 10-11 1.285331713773985E-4 1105 12-13 -0.01046909149302877 1105 14-15 0.09923736337047018 1105 16-17 -0.012126390509408225 1105 18-19 0.06661335001308544 1105 20-21 0.08803593970542778 1105 22-23 0.02266472479438164 1105 24-25 -0.007152434126325602 1105 26-27 -0.022219314721461103 1105 28-29 -0.09486752077317107 1105 30-31 0.0773047842508845 1105 32-33 0.13115356654493837 1105 34-35 0.054034929628684836 1105 36-37 0.13757805707645332 1105 38-39 0.1025494823156663 1105 40-41 8.790338138240372E-4 1105 42-43 -0.06421669279556141 1105 44-45 0.1731500942407891 1105 46-47 0.14210004835209133 1105 48-49 0.240634551858669 1105 50-51 0.3564758961729879 1105 52-53 0.21215729588959675 1105 54-55 0.01572923566094886 1105 56-57 0.2837938427106508 1105 58-59 0.3841569205299109 1105 60-61 0.396057824414882 1105 62-63 0.32234631726492324 1105 64-65 0.47169810189789985 1105 66-67 0.47003987536538006 1105 68-69 0.3405076250659249 1105 70-71 0.3114492405671996 1105 72-73 0.43226792284743 1105 74-75 0.5311282479397121 1105 76-77 0.302317274869047 1105 78-79 0.3173331595248605 1105 80-81 0.5167652842263273 1105 82-83 0.516514872570891 1105 84-85 0.4276143105490462 1105 86-87 0.5453499939307029 1105 88-89 0.676328691168333 1105 90-91 0.7236842395614929 1105 92-93 0.970647785351975 1105 94-95 1.009656304719151 1105 96-97 0.9079457667773987 1105 98-99 1.3862269762350863 1105 100-101 2.0200085001213104 1106 1 -0.07004758049370707 1106 2 0.04582431767025241 1106 3 -0.028285303674657314 1106 4 0.043087734718895376 1106 5 -0.02010422218796748 1106 6 -0.02294072148281856 1106 7 -0.07133355574818268 1106 8 -0.1048989436514205 1106 9 -0.024478362823330713 1106 10-11 -0.0020189182786651827 1106 12-13 -0.17880037717633712 1106 14-15 -0.06487748398496507 1106 16-17 0.046724619721963734 1106 18-19 0.1908614838984164 1106 20-21 0.11432105518256463 1106 22-23 0.08396878533599761 1106 24-25 0.07782831108062993 1106 26-27 -0.04526828280909001 1106 28-29 0.2064715390973646 1106 30-31 0.1560661796813534 1106 32-33 0.06961462850097888 1106 34-35 -0.13592883212809426 1106 36-37 0.0629406976157938 1106 38-39 0.11968056977269725 1106 40-41 0.2170690626185845 1106 42-43 0.16556267721651352 1106 44-45 0.21821353697713874 1106 46-47 0.176377193480576 1106 48-49 0.17208748860177536 1106 50-51 0.08727582183813354 1106 52-53 0.3474560290997317 1106 54-55 0.3016546530202078 1106 56-57 0.30282046904377324 1106 58-59 0.20224661792509124 1106 60-61 0.24622662648799576 1106 62-63 0.3430702561864507 1106 64-65 0.40741961461225173 1106 66-67 0.43562077130690824 1106 68-69 0.29402149574391956 1106 70-71 0.4019710299724153 1106 72-73 0.48402047036496043 1106 74-75 0.4249822005925381 1106 76-77 0.3523157262261769 1106 78-79 0.4896274223192343 1106 80-81 0.5639240407693435 1106 82-83 0.41471276848144 1106 84-85 0.5030987776610303 1106 86-87 0.5207936801137123 1106 88-89 0.4353309521869271 1106 90-91 0.5066791083914381 1106 92-93 0.8522483593822692 1106 94-95 0.6186962493809816 1106 96-97 0.8637118804064876 1106 98-99 1.096878541913032 1106 100-101 1.930278583438298 1107 1 0.1541706292642644 1107 2 0.2050149950937339 1107 3 0.09676784013376505 1107 4 0.04886056094504454 1107 5 -0.16885566283337994 1107 6 -0.02052043719545793 1107 7 0.06221491396625112 1107 8 0.11626125611807936 1107 9 0.1332191656694306 1107 10-11 0.08713590308129682 1107 12-13 0.03402456839928192 1107 14-15 0.14973514247169106 1107 16-17 0.08644801580340555 1107 18-19 0.15122388625466954 1107 20-21 0.046718289143377945 1107 22-23 0.1541583102431474 1107 24-25 0.17268552711341556 1107 26-27 -0.024925089058292826 1107 28-29 0.07130186308251041 1107 30-31 0.03091827341934561 1107 32-33 0.009277837618661522 1107 34-35 0.03951744780531641 1107 36-37 0.1534119487362986 1107 38-39 0.1012390279619666 1107 40-41 0.08672458829408214 1107 42-43 0.09546791476293492 1107 44-45 0.032143617653282774 1107 46-47 -0.07692796723142692 1107 48-49 -0.007451504867304948 1107 50-51 0.01758187895171659 1107 52-53 0.00946268808295514 1107 54-55 -0.029747575178028285 1107 56-57 0.12062428515339008 1107 58-59 0.05305465992790914 1107 60-61 0.17470146321485203 1107 62-63 0.15420740562939983 1107 64-65 0.28688433480689923 1107 66-67 0.2776107828320633 1107 68-69 0.18308411579769768 1107 70-71 0.21679751223647514 1107 72-73 0.1968466965142781 1107 74-75 0.2455879119512261 1107 76-77 0.20538782252531362 1107 78-79 0.33162383671288254 1107 80-81 0.24165870589932226 1107 82-83 0.3173866063608095 1107 84-85 0.33447155266423323 1107 86-87 0.5014275640937349 1107 88-89 0.6426737617656215 1107 90-91 0.6375959991585063 1107 92-93 0.8449823842253998 1107 94-95 0.8555537215285014 1107 96-97 0.9937528587647968 1107 98-99 1.1380528279931958 1107 100-101 1.6755225327275802 1108 1 0.04123702944192331 1108 2 0.11793823145467996 1108 3 0.04906334683300173 1108 4 0.05506531333233511 1108 5 -0.1563973017407747 1108 6 -0.04972319091519495 1108 7 -0.04636307108075499 1108 8 0.024674906277226683 1108 9 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-0.96673953254545 2316 92-93 -0.9762193917132187 2316 94-95 -0.9519948797043085 2316 96-97 -1.2161027384592238 2316 98-99 -1.5068491074350554 2316 100-101 -1.7433558672034515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 8.0 5 34.0 6 49.0 7 114.0 8 292.0 9 537.0 10 939.0 11 1571.0 12 2565.0 13 3982.0 14 5014.0 15 7309.0 16 10529.0 17 15400.0 18 13762.0 19 7400.0 20 5942.0 21 6157.0 22 6169.0 23 6481.0 24 7446.0 25 8927.0 26 10709.0 27 13401.0 28 16720.0 29 21547.0 30 27052.0 31 34959.0 32 45842.0 33 62060.0 34 87821.0 35 131380.0 36 208846.0 37 324004.0 38 253205.0 39 19970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6338069777237 19.13835817577686 15.032700767502808 22.19513407899663 2 15.7322276041975 20.11618634547311 40.04672012583443 24.104865924494955 3 19.32051161169085 24.59034007310618 29.938522687986012 26.150625627216957 4 12.89225499891459 16.757629114415337 36.43826284748642 33.911853039183654 5 13.630605189805776 37.794628608773266 33.42648969151673 15.14827650990423 6 32.27781809995564 35.892926710360804 16.90015765849722 14.929097531186342 7 29.444716101413075 33.29532572157816 19.482190144772453 17.777768032236303 8 27.601273839726286 34.43562716579432 18.80499683148083 19.158102162998567 9 27.465104261457284 16.540327903361263 19.948587395945026 36.04598043923642 10-11 25.62670531748416 26.837613209691646 26.115870589929298 21.419810882894893 12-13 26.356586030838724 24.071969212439285 27.543719871206836 22.027724885515156 14-15 23.08962414126552 25.286537192275393 26.24103988825762 25.382798778201465 16-17 22.09210706159499 28.953211899013777 26.715915760514036 22.238765278877196 18-19 22.006626480926393 26.518787820314632 30.050900963127503 21.42368473563148 20-21 22.77857143118184 25.623042286072646 30.231397172303677 21.366989110441835 22-23 22.39448304090575 25.585847991258237 30.271718275475184 21.747950692360824 24-25 22.33327339785899 25.84962189486733 30.424087336219007 21.393017371054672 26-27 22.01776454954909 26.29268820379529 30.25565046289867 21.433896783756957 28-29 21.601323289277293 26.33264527748581 30.565979536673325 21.500051896563573 30-31 21.756585138555153 25.881609121890158 30.54842806369974 21.813377675854948 32-33 21.867058145067745 26.045629599743304 30.244959057762067 21.842353197426885 34-35 22.340764552347082 25.970016379818833 29.93702431098413 21.752194756849956 36-37 22.458129468638578 25.699450204875795 29.978927687542118 21.863492638943505 38-39 21.936641311204134 26.04234047949526 30.18849582683732 21.83252238246329 40-41 21.90674686272747 26.09204274102125 30.1137962514262 21.887414144825083 42-43 22.14043287600337 25.870477839026385 29.75311638120398 22.235972903766267 44-45 22.024826279632233 26.214069102223664 29.566121184346418 22.194983433797685 46-47 22.27479941848354 26.718656694054076 28.922330714462703 22.084213172999682 48-49 22.62918385231996 26.67304755994787 28.65795853808107 22.0398100496511 50-51 22.633130796617614 26.78553547243096 28.340887346169673 22.240446384781755 52-53 22.795593452745543 27.07031998046992 28.00614846661102 22.12793810017352 54-55 22.354103762241923 26.879238853719666 28.21761842842911 22.5490389556093 56-57 22.478030504032095 27.365041914355693 28.118506271621396 22.038421309990813 58-59 22.745837983783908 26.715440665367097 28.22748578917324 22.311235561675755 60-61 22.47258518273256 26.733640464072938 28.91699503050476 21.87677932268974 62-63 22.322784028324442 26.916479004083627 28.704664045603284 22.056072921988644 64-65 22.26149675436923 26.725636938136034 28.46342534829956 22.549440959195174 66-67 22.27816163029265 27.104909048515985 28.197372066013376 22.41955725517799 68-69 22.25458960184834 26.81444318483321 28.49094432104153 22.440022892276925 70-71 22.33290720953231 27.052210032986224 28.423115352370758 22.191767405110706 72-73 22.520642884134524 26.8865114640459 28.15724479898724 22.435600852832334 74-75 22.29841614183861 27.290388858210722 27.909218786146074 22.50197621380459 76-77 22.331628107213625 27.17990099017138 28.07026584131676 22.418205061298238 78-79 22.453873743099244 26.929964397100605 28.07735572274032 22.538806137059833 80-81 22.185920080225298 27.691432280303214 28.01961338949689 22.103034249974602 82-83 22.282709593859135 27.489427297963815 27.768235261441397 22.459627846735657 84-85 22.180133618829284 27.211871836730815 27.789990172828837 22.81800437161106 86-87 22.202658047709786 27.04515669734319 27.967279831772462 22.784905423174557 88-89 22.268148086426383 27.14744833705735 27.7936508284563 22.790752748059965 90-91 22.19169240564112 27.160876905022178 28.066647588576977 22.580783100759724 92-93 22.268547163667343 26.631014166587786 28.258839635746597 22.841599033998275 94-95 22.038055852185472 26.762401993353052 28.267101415271895 22.932440739189573 96-97 22.147985560031195 26.7181450531266 28.14449032158094 22.98937906526126 98-99 22.25405768097849 26.88207252734723 27.768381444670382 23.0954883470039 100-101 21.904797061996895 26.781025494077504 28.251562129935294 23.06261531399031 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 410.0 1 302.0 2 291.0 3 388.5 4 311.5 5 271.5 6 556.0 7 1308.0 8 2109.0 9 2404.0 10 2220.0 11 2009.0 12 2080.0 13 2322.0 14 2475.0 15 2551.0 16 2749.0 17 2815.0 18 2814.0 19 2961.5 20 3113.0 21 3358.5 22 3822.0 23 4485.0 24 5211.0 25 6172.5 26 7640.5 27 9296.5 28 10456.0 29 11995.0 30 14391.5 31 16561.0 32 18793.0 33 21339.0 34 24811.5 35 27598.0 36 30662.5 37 34284.0 38 35846.5 39 36441.5 40 37918.0 41 41724.0 42 45616.0 43 48356.0 44 50604.0 45 52660.5 46 52576.0 47 52705.5 48 55663.0 49 55064.0 50 51816.0 51 48844.5 52 47092.5 53 46695.0 54 45631.5 55 42047.0 56 36255.0 57 32378.5 58 29894.5 59 26602.5 60 22838.5 61 18942.0 62 15527.0 63 12361.0 64 9218.5 65 7040.5 66 5503.5 67 4758.5 68 3501.0 69 2004.5 70 1503.0 71 1180.5 72 1033.5 73 876.0 74 687.0 75 608.0 76 412.5 77 216.5 78 137.0 79 89.0 80 52.5 81 35.0 82 17.5 83 9.5 84 9.5 85 5.0 86 4.0 87 4.0 88 2.5 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04348947883524212 2 0.0 3 0.0 4 0.0 5 0.014325945969256227 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004531676786193296 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0963734160145072E-4 22-23 1.4618312213526764E-4 24-25 6.578240496087043E-4 26-27 0.0021196552709613807 28-29 0.002996754003772986 30-31 6.943698301425211E-4 32-33 0.0 34-35 0.0 36-37 7.309156106763382E-5 38-39 0.0 40-41 0.0 42-43 0.009794269183062932 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.309156106763382E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.654578053381691E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.309156106763382E-5 84-85 1.0963734160145072E-4 86-87 0.0 88-89 0.0 90-91 0.006249328471282691 92-93 0.002448567295765733 94-95 0.0 96-97 0.0 98-99 7.309156106763382E-5 100-101 0.010452093232671635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1368147.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.35811692975594 #Duplication Level Percentage of deduplicated Percentage of total 1 73.26363004362108 34.69627558304187 2 14.134869070112932 13.388015644183977 3 5.008756744403193 7.116158627224503 4 2.394110046763074 4.535221741492364 5 1.3096900842038441 3.101222807473378 6 0.7914292286150845 2.2488358770227825 7 0.5426236479000662 1.7988343916271434 8 0.3757640805230065 1.4236383410728617 9 0.3017972847560495 1.286329599050386 >10 1.6334190676103661 14.46559910814843 >50 0.14813588500805253 4.813928548839187 >100 0.09018667191412505 7.698138269943625 >500 0.004191108426893783 1.2820531745853168 >1k 0.0010865836662317216 1.0495340122451013 >5k 3.1045247606620617E-4 1.0962142740490965 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8171 0.5972311454836359 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6741 0.49271021315691954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4647 0.33965648428129436 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2424 0.17717394402794437 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1891 0.13821614197889553 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1705 0.12462111162031565 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.6545780533816904E-5 0.0 22-23 0.0 0.0 0.0 1.8272890266908453E-4 0.0 24-25 0.0 0.0 0.0 2.9236624427053524E-4 0.0 26-27 0.0 0.0 0.0 3.6545780533816906E-4 0.0 28-29 0.0 0.0 0.0 8.770987328116058E-4 0.0 30-31 0.0 0.0 0.0 0.0017541974656232117 0.0 32-33 0.0 0.0 0.0 0.0026678419789686342 0.0 34-35 0.0 0.0 0.0 0.0031794829064420707 0.0 36-37 0.0 0.0 0.0 0.00412967320032131 0.0 38-39 0.0 0.0 0.0 0.005408775519004903 0.0 40-41 0.0 0.0 0.0 0.00804007171743972 0.0 42-43 0.0 0.0 0.0 0.011073371501746523 0.0 44-45 0.0 0.0 0.0 0.017688157778367384 0.0 46-47 0.0 0.0 0.0 0.031356279698014904 0.0 48-49 0.0 0.0 0.0 0.040529270612002954 0.0 50-51 0.0 0.0 0.0 0.055878498436206056 0.0 52-53 0.0 0.0 0.0 0.07627104397407589 0.0 54-55 0.0 0.0 0.0 0.0998796181989216 7.309156106763381E-5 56-57 0.0 0.0 0.0 0.12491347786458618 7.309156106763381E-5 58-59 0.0 0.0 0.0 0.15747576832021704 7.309156106763381E-5 60-61 0.0 0.0 0.0 0.2044370963061718 7.309156106763381E-5 62-63 0.0 0.0 0.0 0.258890309301559 7.309156106763381E-5 64-65 0.0 0.0 0.0 0.30508417589630354 7.309156106763381E-5 66-67 0.0 0.0 0.0 0.38201304391998814 7.309156106763381E-5 68-69 0.0 0.0 0.0 0.4788228165540691 7.309156106763381E-5 70-71 0.0 0.0 0.0 0.5498312681312754 7.309156106763381E-5 72-73 0.0 0.0 0.0 0.6687877837688494 7.309156106763381E-5 74-75 0.0 0.0 0.0 0.776634382124143 7.309156106763381E-5 76-77 0.0 7.309156106763381E-5 0.0 0.8779758315444174 7.309156106763381E-5 78-79 0.0 7.309156106763381E-5 0.0 1.0006965625769746 7.309156106763381E-5 80-81 0.0 7.309156106763381E-5 0.0 1.1293742558365438 7.309156106763381E-5 82-83 0.0 7.309156106763381E-5 0.0 1.2848034604468672 7.309156106763381E-5 84-85 0.0 7.309156106763381E-5 0.0 1.4875229050679497 7.309156106763381E-5 86-87 0.0 7.309156106763381E-5 0.0 1.7512738031805062 7.309156106763381E-5 88-89 0.0 7.309156106763381E-5 0.0 2.060524198057665 7.309156106763381E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4965 0.0 41.441475 1 GTATCAA 8050 0.0 36.83454 1 GGTCGAC 55 0.0025789202 34.547848 7 GTACCGT 95 3.492288E-5 29.998789 6 CCGCTAA 65 0.005842479 29.232794 9 ATCAACG 10340 0.0 28.622469 3 CGTAAGC 100 4.950216E-5 28.503016 3 TCAACGC 10420 0.0 28.357128 4 TATCAAC 10465 0.0 28.280584 2 CAACGCA 10640 0.0 27.815443 5 AACGCAG 10830 0.0 27.323458 6 GTACATG 7955 0.0 24.789885 1 ACGCAGA 12280 0.0 23.983746 7 CGCAGAG 12310 0.0 23.848118 8 TACATGG 8250 0.0 23.781305 2 GGACCGA 345 0.0 23.404852 6 ACATGGG 8220 0.0 22.712257 3 GAGTACT 6950 0.0 22.658045 12-13 GCAGAGT 13050 0.0 22.277405 9 AGTACTT 7110 0.0 22.248375 12-13 >>END_MODULE