FastQCFastQC Report
Thu 26 May 2016
SRR937603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883797
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82150.4360873278808704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.3248226852468711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.22014049284503584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25510.13541798824395623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22900.12156299219077216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103100.031.6928371
GGTATCA69650.030.1831251
GTACATG95800.027.6040171
TACATGG97550.026.6099072
ATCAACG123700.026.0578173
TCAACGC124750.025.801074
CAACGCA125250.025.6601095
ACATGGG98650.025.493923
TATCAAC126750.025.4307822
AACGCAG127300.025.3393696
CGTATAG1050.00218006122.6487261
ACGCAGA143400.022.2968467
GAGTACT67650.022.22476812-13
CGCAGAG144600.022.1112218
AGTACTT73200.021.41579212-13
AGAGTAC120600.020.89657810-11
CATGGGG38050.020.7416344
CATGGGA57350.020.7250774
GCAGAGT152600.020.5462369
GTACTTT73500.020.4547714-15
Produced by FastQC (version 0.11.5)