##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937603_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1883797 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6466275293994 33.0 31.0 34.0 30.0 34.0 2 31.784630191045 33.0 31.0 34.0 30.0 34.0 3 31.803675767611903 33.0 31.0 34.0 30.0 34.0 4 35.42265222845137 37.0 35.0 37.0 33.0 37.0 5 35.41432065132283 37.0 35.0 37.0 33.0 37.0 6 35.48387273150982 37.0 35.0 37.0 33.0 37.0 7 35.414268097889526 37.0 35.0 37.0 33.0 37.0 8 35.401695087103334 37.0 35.0 37.0 33.0 37.0 9 37.01563385014415 39.0 37.0 39.0 33.0 39.0 10-11 36.88368306139144 39.0 37.0 39.0 33.0 39.0 12-13 36.759641298929765 39.0 37.0 39.0 33.0 39.0 14-15 37.88083907130121 40.0 38.0 41.0 33.0 41.0 16-17 37.788304684634284 40.0 38.0 41.0 33.0 41.0 18-19 37.72251282914242 40.0 37.0 41.0 32.5 41.0 20-21 37.611626146554 40.0 37.0 41.0 32.0 41.0 22-23 37.52648454159339 40.0 37.0 41.0 32.0 41.0 24-25 37.55612122749957 40.0 37.0 41.0 32.0 41.0 26-27 37.473684266404504 40.0 37.0 41.0 32.0 41.0 28-29 37.34646859507686 40.0 37.0 41.0 32.0 41.0 30-31 37.1038596515442 39.0 36.5 41.0 31.0 41.0 32-33 36.952053751014574 39.0 36.0 41.0 31.0 41.0 34-35 36.933114608421185 39.0 36.0 41.0 31.0 41.0 36-37 36.77740993323591 39.0 36.0 41.0 30.0 41.0 38-39 36.60817195271041 39.0 36.0 41.0 30.0 41.0 40-41 36.36685295708614 39.0 35.0 41.0 30.0 41.0 42-43 36.07395356293698 39.0 35.0 40.0 29.5 41.0 44-45 35.81922786797091 39.0 35.0 40.0 28.5 41.0 46-47 35.441093971378024 38.5 35.0 40.0 27.0 41.0 48-49 35.10820725375399 38.0 34.5 40.0 26.0 41.0 50-51 34.22024161839094 37.5 33.5 39.5 24.0 40.5 52-53 34.325339195252994 38.0 33.5 39.5 24.0 40.5 54-55 34.59219438187873 38.0 34.0 40.0 24.0 41.0 56-57 34.339803333374036 38.0 34.0 40.0 23.5 41.0 58-59 34.065836446283754 38.0 33.5 40.0 23.0 41.0 60-61 33.719202759108335 37.5 33.0 40.0 22.0 41.0 62-63 33.32140511955375 37.0 33.0 40.0 20.5 41.0 64-65 32.9101044326963 36.0 32.0 39.0 19.5 41.0 66-67 32.44845304456903 36.0 31.5 39.0 18.0 40.0 68-69 31.917279834292124 35.0 31.0 38.5 13.5 40.0 70-71 31.43818336052133 35.0 31.0 38.0 9.5 40.0 72-73 30.95808943320326 35.0 30.0 37.0 7.5 39.5 74-75 30.400679850323577 34.5 29.5 37.0 4.5 39.0 76-77 29.88780850590589 34.0 29.0 36.0 2.0 39.0 78-79 29.301109408285498 34.0 29.0 36.0 2.0 38.0 80-81 28.71669983549183 34.0 28.0 35.0 2.0 37.0 82-83 28.184235084778244 33.5 26.5 35.0 2.0 36.5 84-85 27.637882160338933 33.0 26.0 35.0 2.0 36.0 86-87 27.065472022728564 33.0 25.5 35.0 2.0 36.0 88-89 26.53293666992781 32.0 25.0 35.0 2.0 35.0 90-91 25.926586304150604 32.0 23.5 34.5 2.0 35.0 92-93 25.234486518451828 31.5 20.0 34.0 2.0 35.0 94-95 24.425304584304996 31.0 15.5 34.0 2.0 35.0 96-97 23.507839751310783 31.0 3.5 34.0 2.0 35.0 98-99 22.36330400780976 30.5 2.0 34.0 2.0 35.0 100-101 19.515246600350252 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16209167557442328 1101 2 0.21256098959769432 1101 3 0.2792565786679475 1101 4 0.19790159760522386 1101 5 0.077815201801549 1101 6 0.24814386202995564 1101 7 0.29963063398825796 1101 8 0.3273032936151452 1101 9 0.36597484489212917 1101 10-11 0.40193792013264584 1101 12-13 0.4155072325889506 1101 14-15 0.4887619690863758 1101 16-17 0.511587984846706 1101 18-19 0.5367102278089249 1101 20-21 0.6167465592745529 1101 22-23 0.5214796848114389 1101 24-25 0.48484846641517976 1101 26-27 0.4973290728424473 1101 28-29 0.6063865301053752 1101 30-31 0.684732528732205 1101 32-33 0.4844885191167023 1101 34-35 0.6537007366900127 1101 36-37 0.629544065676491 1101 38-39 0.5895389702154503 1101 40-41 0.70400970703826 1101 42-43 0.7680437156777984 1101 44-45 0.63140534906303 1101 46-47 0.4526058428065838 1101 48-49 0.7491056751013687 1101 50-51 0.7185206345895878 1101 52-53 0.6776006610068208 1101 54-55 0.7781126041040096 1101 56-57 0.6878342489286595 1101 58-59 0.7555186798149265 1101 60-61 0.8106452357708989 1101 62-63 0.8422081701186954 1101 64-65 0.8718846185384095 1101 66-67 0.9735103639209939 1101 68-69 0.8416978657902696 1101 70-71 0.9371057496827966 1101 72-73 0.8229006118236697 1101 74-75 1.1309360464616596 1101 76-77 0.9896099005624706 1101 78-79 0.9810239310188926 1101 80-81 1.1726197610864553 1101 82-83 1.1648744291284423 1101 84-85 1.3443527592753242 1101 86-87 1.4428596821002415 1101 88-89 1.501840681790238 1101 90-91 1.5297116944779816 1101 92-93 1.4096374053829024 1101 94-95 1.5985997968309285 1101 96-97 2.010632106790414 1101 98-99 2.2458909709214687 1101 100-101 2.3456452503759344 1102 1 0.18080210602043323 1102 2 0.3059652912218489 1102 3 0.2688257821514881 1102 4 0.0845342777511533 1102 5 0.05681914119172404 1102 6 0.1040159687067117 1102 7 0.14641343348212388 1102 8 0.13729041085652938 1102 9 0.1575462769686382 1102 10-11 0.03676444899235065 1102 12-13 0.2647504382322481 1102 14-15 0.32661318995318567 1102 16-17 0.42327895286349815 1102 18-19 0.48198479180098275 1102 20-21 0.4780719316175066 1102 22-23 0.4375389372163241 1102 24-25 0.4448593385330071 1102 26-27 0.2960154966836157 1102 28-29 0.5218422716319253 1102 30-31 0.2864894336582111 1102 32-33 0.382963108727715 1102 34-35 0.4254947604028345 1102 36-37 0.5869121082141291 1102 38-39 0.6081858312738007 1102 40-41 0.618839473316406 1102 42-43 0.5513852222181086 1102 44-45 0.5276489558785542 1102 46-47 0.5420173814629834 1102 48-49 0.5608603080988885 1102 50-51 0.5582542422112908 1102 52-53 0.7500989188232268 1102 54-55 0.6040033801061995 1102 56-57 0.6156696109061315 1102 58-59 0.5966837935722609 1102 60-61 0.6576361095010697 1102 62-63 0.723165330724278 1102 64-65 0.6616753111515834 1102 66-67 0.5954209952398983 1102 68-69 0.5894747694225835 1102 70-71 0.775222559946183 1102 72-73 0.7914296225660991 1102 74-75 0.7050061728962298 1102 76-77 0.6716318404452686 1102 78-79 0.6980724441803083 1102 80-81 0.7052063214026489 1102 82-83 0.8105405741756222 1102 84-85 0.9263961438688462 1102 86-87 0.9466872283887895 1102 88-89 1.0431010381038277 1102 90-91 1.1822939977163855 1102 92-93 1.163622759888188 1102 94-95 1.4537074776380372 1102 96-97 1.4035806206847035 1102 98-99 1.541534667530751 1102 100-101 1.8054912244581054 1103 1 0.4806554538324015 1103 2 0.40627454065733914 1103 3 0.3292424292776417 1103 4 0.286798113115843 1103 5 0.26640771932508756 1103 6 0.18579718010159496 1103 7 0.26393487535118254 1103 8 0.24948069623073366 1103 9 0.24432431296695256 1103 10-11 0.27570888000636984 1103 12-13 0.3750510676536365 1103 14-15 0.33441462040958214 1103 16-17 0.48129355393898976 1103 18-19 0.45145151579322373 1103 20-21 0.41218979011433277 1103 22-23 0.3240700214591925 1103 24-25 0.4091221336868003 1103 26-27 0.3380546307609862 1103 28-29 0.30255443789381076 1103 30-31 0.4332216495464465 1103 32-33 0.5206120868672528 1103 34-35 0.4546530397766091 1103 36-37 0.30749491072625545 1103 38-39 0.4118917111884386 1103 40-41 0.6377355790003776 1103 42-43 0.5720402767263124 1103 44-45 0.562695561139094 1103 46-47 0.6783493037442767 1103 48-49 0.712569984571509 1103 50-51 0.6358520690842511 1103 52-53 0.64753376116834 1103 54-55 0.6016430877216123 1103 56-57 0.5647606971338135 1103 58-59 0.6235931236710073 1103 60-61 0.6019547214397676 1103 62-63 0.8344223611490875 1103 64-65 0.9565743444331858 1103 66-67 0.819684625467012 1103 68-69 0.8171885232314757 1103 70-71 0.956843594595977 1103 72-73 0.7759046715737092 1103 74-75 0.5520296659897816 1103 76-77 0.7888321119046111 1103 78-79 0.9519631748361839 1103 80-81 0.9674654851977422 1103 82-83 0.9126871361999385 1103 84-85 1.0802779186659102 1103 86-87 1.0515750093599507 1103 88-89 1.1015962601834097 1103 90-91 1.077375809184062 1103 92-93 1.0272052123351507 1103 94-95 1.0178199293376693 1103 96-97 1.233430498101388 1103 98-99 1.4336823762680702 1103 100-101 1.529478312288152 1104 1 0.23936090764790308 1104 2 0.27695278007592705 1104 3 0.22667267382024647 1104 4 0.16854822835118455 1104 5 0.29035897615182193 1104 6 0.18063138769578302 1104 7 0.3103924871642789 1104 8 0.251134167519659 1104 9 0.25957250916031427 1104 10-11 0.23594289550780445 1104 12-13 0.4298529078221449 1104 14-15 0.5081212069979628 1104 16-17 0.34608848143717097 1104 18-19 0.2800467352886358 1104 20-21 0.3426385509246188 1104 22-23 0.37362571631889807 1104 24-25 0.3004058162748464 1104 26-27 0.42170098748204765 1104 28-29 0.3817886370175856 1104 30-31 0.30777133244256305 1104 32-33 0.29078218845130266 1104 34-35 0.27981164973520123 1104 36-37 0.4088344255281555 1104 38-39 0.28016647054310084 1104 40-41 0.25585231581386836 1104 42-43 0.24863666699543074 1104 44-45 0.28510093834375994 1104 46-47 0.16325383608313615 1104 48-49 0.2355935569803549 1104 50-51 0.25539572261181576 1104 52-53 0.2590280553870272 1104 54-55 0.24495423069669187 1104 56-57 0.3713574964195132 1104 58-59 0.2986058075362834 1104 60-61 0.5091665540284112 1104 62-63 0.3861522257123866 1104 64-65 0.5059308975315204 1104 66-67 0.45200177982551537 1104 68-69 0.4676095927489179 1104 70-71 0.5651443685655799 1104 72-73 0.5305330132899471 1104 74-75 0.533280026780119 1104 76-77 0.23240261780590643 1104 78-79 0.42693223026605054 1104 80-81 0.5059297236596301 1104 82-83 0.6784743785376186 1104 84-85 0.7227507226892413 1104 86-87 0.8275657650050334 1104 88-89 0.9729090554694473 1104 90-91 0.9919853303358757 1104 92-93 0.8896007209269641 1104 94-95 1.0276580835067541 1104 96-97 0.9857420944757003 1104 98-99 1.1007124198413898 1104 100-101 0.9558148609946322 1105 1 0.2628274335218208 1105 2 0.31687379896169077 1105 3 0.31212315079054065 1105 4 0.2675468041247555 1105 5 0.26604622405398715 1105 6 0.2551145579972527 1105 7 0.2140784326581553 1105 8 0.2111721657339345 1105 9 0.29038373598766043 1105 10-11 0.367312309432549 1105 12-13 0.2530201737341784 1105 14-15 0.34734415990917 1105 16-17 0.3797255498766319 1105 18-19 0.4279812960551084 1105 20-21 0.41666186482452616 1105 22-23 0.4601726978435181 1105 24-25 0.3813298026052152 1105 26-27 0.30183943054450424 1105 28-29 0.43107905343494224 1105 30-31 0.4378835605810778 1105 32-33 0.3959602789364567 1105 34-35 0.3919309715897441 1105 36-37 0.5385477063336594 1105 38-39 0.4771883703916373 1105 40-41 0.4981532540961666 1105 42-43 0.3748454858936938 1105 44-45 0.3812362226138717 1105 46-47 0.5100515594689128 1105 48-49 0.5781169052211013 1105 50-51 0.42109382674286167 1105 52-53 0.5159126879583624 1105 54-55 0.5396761136715753 1105 56-57 0.6194748974479225 1105 58-59 0.5575192349757785 1105 60-61 0.7243161797697439 1105 62-63 0.8159312770063067 1105 64-65 0.7884981003016236 1105 66-67 0.8172964991647191 1105 68-69 0.8328251726951059 1105 70-71 0.9339889136426685 1105 72-73 1.0753678818803998 1105 74-75 0.9273568954378391 1105 76-77 0.8317629736618386 1105 78-79 0.8517807560546125 1105 80-81 0.6773982201044326 1105 82-83 0.8874173560611851 1105 84-85 0.6703050469363347 1105 86-87 0.7579221099069429 1105 88-89 0.7969724336870634 1105 90-91 1.0482255986636417 1105 92-93 1.2495141028613048 1105 94-95 1.325480110882765 1105 96-97 1.5504190460602025 1105 98-99 1.684311035015071 1105 100-101 1.6422657164208054 1106 1 0.11049480032620451 1106 2 0.13712335177172008 1106 3 0.04197436882062533 1106 4 0.19389629079699944 1106 5 0.16117253954212174 1106 6 0.05221175574450143 1106 7 0.1663757097353198 1106 8 0.26600130094630003 1106 9 0.10694480158211661 1106 10-11 0.09246895503999752 1106 12-13 -0.10323167633816865 1106 14-15 0.08749424928814165 1106 16-17 0.06764553290097552 1106 18-19 0.08575753196246438 1106 20-21 0.02678569682571208 1106 22-23 0.060107002751969674 1106 24-25 0.09893431638332828 1106 26-27 0.04260441535583226 1106 28-29 0.06560391944027089 1106 30-31 0.09974257230378925 1106 32-33 0.27374896326682574 1106 34-35 0.35744886937042253 1106 36-37 0.27784281511865316 1106 38-39 0.25477270934289464 1106 40-41 0.3209435483399332 1106 42-43 0.2674366673458408 1106 44-45 0.2740443218211368 1106 46-47 0.3189325560274412 1106 48-49 0.5155188679873888 1106 50-51 0.4511906487944515 1106 52-53 0.4688704574653926 1106 54-55 0.40574858308986705 1106 56-57 0.5237101354568736 1106 58-59 0.3580801813392469 1106 60-61 0.3888903525088807 1106 62-63 0.44521706275471473 1106 64-65 0.5908193268697133 1106 66-67 0.6664499739363023 1106 68-69 0.5439615531670832 1106 70-71 0.4858691478902344 1106 72-73 0.6545220561010687 1106 74-75 0.6253679809680683 1106 76-77 0.6974014600067946 1106 78-79 0.7063725212663776 1106 80-81 0.5022136703150188 1106 82-83 0.3663425418652615 1106 84-85 0.6570392719246101 1106 86-87 0.7457252819823381 1106 88-89 0.8745050370159113 1106 90-91 1.0087728334639756 1106 92-93 1.001468059632991 1106 94-95 1.0027547444024343 1106 96-97 1.1019995796542688 1106 98-99 0.9903601481460917 1106 100-101 0.9593478273168365 1107 1 0.27501100729311645 1107 2 0.22133461038663782 1107 3 0.2718685869865993 1107 4 0.1992978853982592 1107 5 0.2021390224103925 1107 6 0.23808663097980087 1107 7 0.21738304003422826 1107 8 0.25905755792678775 1107 9 0.3029597829514614 1107 10-11 0.461120727855743 1107 12-13 0.35592365829318595 1107 14-15 0.42116504280762257 1107 16-17 0.5132574395209488 1107 18-19 0.5219625660961071 1107 20-21 0.3564238508234041 1107 22-23 0.3417180617433644 1107 24-25 0.46475867155832873 1107 26-27 0.4309409937598687 1107 28-29 0.3328077668356926 1107 30-31 0.44534012861854677 1107 32-33 0.411717105589247 1107 34-35 0.5906010143335365 1107 36-37 0.5552455670589254 1107 38-39 0.6051857681788491 1107 40-41 0.601211558482369 1107 42-43 0.47188863850434615 1107 44-45 0.49611267526478287 1107 46-47 0.5901344042768883 1107 48-49 0.6371705968498702 1107 50-51 0.35765911436260467 1107 52-53 0.2955913952593008 1107 54-55 0.44172418698883575 1107 56-57 0.5105676718558598 1107 58-59 0.48686194521588533 1107 60-61 0.498880056965362 1107 62-63 0.5649160731076037 1107 64-65 0.637662594466228 1107 66-67 0.5779691356689014 1107 68-69 0.7172166011707439 1107 70-71 0.6153908402831441 1107 72-73 0.5984740719405437 1107 74-75 0.49675111461481336 1107 76-77 0.43753125895406697 1107 78-79 0.6198855895042605 1107 80-81 0.7278879381802064 1107 82-83 0.8161234595687326 1107 84-85 0.9015265570593556 1107 86-87 0.8065014442915697 1107 88-89 0.8234115466694725 1107 90-91 1.0014119710251883 1107 92-93 0.7808134877800192 1107 94-95 1.0564311441466323 1107 96-97 1.2498416343152492 1107 98-99 1.3251067779347032 1107 100-101 1.2993547317638878 1108 1 0.14807162710599897 1108 2 0.20129825994033368 1108 3 0.24705874081497825 1108 4 0.24163610119348533 1108 5 0.22761988537798317 1108 6 0.20714597986020777 1108 7 0.2454932556539191 1108 8 0.1733518265656997 1108 9 0.2695953058474103 1108 10-11 0.29180313749483133 1108 12-13 0.45622219966487165 1108 14-15 0.5189930980865185 1108 16-17 0.5349571672814406 1108 18-19 0.6106052457639066 1108 20-21 0.3983160182020953 1108 22-23 0.5269711900536649 1108 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-1.405678104724597 2316 98-99 -1.9213262420283677 2316 100-101 -1.961979273963614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7536.0 3 1389.0 4 1347.0 5 1527.0 6 1734.0 7 2019.0 8 2653.0 9 3427.0 10 4389.0 11 5663.0 12 7343.0 13 9512.0 14 10801.0 15 13600.0 16 16820.0 17 21226.0 18 18415.0 19 13954.0 20 14129.0 21 15051.0 22 16296.0 23 18614.0 24 21053.0 25 24018.0 26 27847.0 27 32486.0 28 38637.0 29 46383.0 30 55953.0 31 68691.0 32 86049.0 33 110502.0 34 145463.0 35 197706.0 36 269174.0 37 328824.0 38 206635.0 39 16930.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90124009085656 18.909721279128288 14.489970362440985 22.69906826757417 2 15.097265699865842 19.84049915884069 41.1417406673907 23.920494473902767 3 19.339496077479364 24.438134055656352 29.619130060733056 26.603239806131228 4 12.41512479596411 16.274903558769257 37.24840897257481 34.06156267269183 5 13.495482870687855 37.871422608221565 33.83773900746679 14.795355513623798 6 32.11897263286896 36.032688857459114 17.25936142474398 14.588977084927945 7 29.014465079293316 33.09516527529926 19.95244794672468 17.937921698682743 8 26.631780457304377 35.11441651857403 19.26658110006452 18.987221924057067 9 26.572397377764183 17.125487015145673 20.38711221626797 35.91500339082218 10-11 25.273839692073967 26.917542762185015 26.692694203832907 21.11592334190811 12-13 26.035753948667324 24.146760220580727 27.689284984852097 22.128200845899855 14-15 22.686659807144416 25.439501859331216 26.32083256764293 25.553005765881437 16-17 21.99898137349683 29.159899094805404 26.704424110330816 22.13669542136695 18-19 21.870107517273528 27.300595468419107 29.501709069624145 21.327587944683216 20-21 22.731220459396113 26.255404198694748 29.564270940422315 21.449104401486824 22-23 22.333772721869714 26.2598161056596 29.553421872295093 21.852989300175587 24-25 22.133048260309966 26.50849368690898 29.665996532320687 21.69246152046037 26-27 21.886707761249355 26.828800670532548 29.526220997592667 21.75827057062543 28-29 21.541026194764452 27.12101740750236 29.703525649549945 21.634430748183238 30-31 21.69490118914537 26.575400178575276 29.736001695853375 21.993696936425977 32-33 21.545984255612634 26.715108616696753 29.633514899769636 22.10539222792098 34-35 22.241502299867385 26.731390277415016 29.36037248968158 21.66673493303601 36-37 22.156887562459982 26.46960440415996 29.367385944722518 22.00612208865754 38-39 21.799602083121183 26.82623401160356 29.48844310168842 21.885720803586835 40-41 22.025482455883786 26.7855082159356 29.393261438468095 21.79574788971252 42-43 22.01170072730657 26.68654017471313 29.119573455304554 22.182185642675744 44-45 21.952165870087153 26.928066370161087 28.993809976161124 22.12595778359064 46-47 22.230782978533277 27.167097689074517 28.50960571821879 22.09251361417342 48-49 22.30933442406793 27.306173651791667 28.24097504439309 22.143516879747306 50-51 22.13354815819947 27.44049009005942 28.21770539170343 22.20825636003768 52-53 22.497440884974964 27.47146134512619 27.949845601833623 22.081252168065223 54-55 22.143721960155798 27.21842352220996 28.200900040606335 22.436954477027903 56-57 22.33881978205757 27.644682319064845 28.00718409714218 22.009313801735402 58-59 22.496514988805156 27.193828838158552 28.05320348503778 22.256452687998507 60-61 22.279275765616813 27.229375183941844 28.52951005469005 21.961838995751293 62-63 22.17494003093771 27.318240964227016 28.402469911775952 22.104349093059326 64-65 22.078486621520053 27.31236375789071 28.328202472225154 22.280947148364085 66-67 22.02592539615777 27.52260283405481 28.04689606179216 22.40457570799526 68-69 22.04652931314821 27.28552416937106 28.30755240497174 22.360394112508992 70-71 22.101037354989387 27.435269006121604 28.197199545665313 22.26649409322369 72-73 22.236490630840024 27.272113352491996 28.181923805102755 22.30947221156523 74-75 21.99253023176914 27.66050336783012 27.9510091259648 22.395957274435947 76-77 22.132231607126617 27.460045319108318 27.925148488946487 22.482574584818575 78-79 22.21244597248203 27.542381106222987 27.886694772276755 22.358478149018232 80-81 21.99590122963111 27.83497397589234 27.880917639601737 22.288207154874815 82-83 22.247686847500365 27.721990455728463 27.75277842842554 22.277544268345633 84-85 22.137100569094496 27.594463146040294 27.723643289465077 22.544792995400133 86-87 22.242001444627615 27.51058547295869 27.834204390083485 22.41320869233021 88-89 22.232446204233685 27.568062076261217 27.61849664571563 22.58099507378947 90-91 22.13318592623849 27.635692830628344 27.80998098118778 22.421140261945386 92-93 22.4509496363095 27.403972946531113 27.79536560466204 22.34971181249734 94-95 22.162409639707402 27.21668364266338 27.90154613177255 22.71936058585667 96-97 22.27315640602231 27.237741609839837 27.84221652144575 22.646885462692097 98-99 22.1458844049284 27.359281691863462 27.574088155998577 22.920745747209565 100-101 22.559733884603762 26.59814241288557 28.17462733025747 22.667496372253208 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2417.0 1 1396.0 2 403.5 3 434.0 4 368.5 5 363.0 6 630.5 7 1227.5 8 2008.0 9 2423.0 10 2169.5 11 1888.0 12 2023.0 13 2264.0 14 2396.0 15 2501.5 16 2692.0 17 2823.0 18 2907.0 19 3150.0 20 3508.5 21 4065.5 22 4817.5 23 5794.0 24 7054.0 25 8793.5 26 11185.0 27 13918.0 28 16085.5 29 18511.5 30 22149.0 31 25639.5 32 29434.5 33 33489.5 34 37551.5 35 41548.0 36 46032.5 37 50559.5 38 53073.0 39 53893.5 40 55282.5 41 59065.0 42 62227.0 43 65121.0 44 68705.5 45 71406.5 46 71553.0 47 72849.5 48 77820.5 49 76895.0 50 71437.0 51 67123.0 52 63421.5 53 61732.5 54 59798.0 55 55149.5 56 49596.5 57 44706.0 58 40237.0 59 35217.0 60 29458.5 61 23833.5 62 19034.0 63 14558.0 64 10902.0 65 8508.0 66 6420.5 67 4966.0 68 3651.0 69 2491.0 70 1830.5 71 1372.0 72 1162.0 73 1016.0 74 822.5 75 636.0 76 468.0 77 305.0 78 187.5 79 128.0 80 88.5 81 63.5 82 39.5 83 29.5 84 23.0 85 18.0 86 16.0 87 12.5 88 7.5 89 4.0 90 2.5 91 2.0 92 4.0 93 3.0 94 2.5 95 4.0 96 1.5 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32445109531441024 2 0.2886192089699686 3 0.2873451863443885 4 0.28936238883489035 5 0.29796204155755635 6 0.20002155221608273 7 0.19917220379902928 8 0.2006585635288728 9 0.19757967551705413 10-11 0.20517072699446914 12-13 0.19476620888556465 14-15 0.19564209944065097 16-17 0.20248997105314423 18-19 0.1953766780603218 20-21 0.1882368429294664 22-23 0.18752020520257756 24-25 0.18988245548750743 26-27 0.1858215083684707 28-29 0.2018795018783871 30-31 0.20795765148792575 32-33 0.20981560115023007 34-35 0.20734718231316857 36-37 0.18011494869139297 38-39 0.17270969218020837 40-41 0.1786816732376153 42-43 0.1896170341071782 44-45 0.19508471454195966 46-47 0.20018080504428026 48-49 0.19739388055082366 50-51 0.202569597467243 52-53 0.22513041479522478 54-55 0.18836955361963098 56-57 0.17276277645627422 58-59 0.17217884941954997 60-61 0.16495938787459583 62-63 0.1722053915575829 64-65 0.16899379285559962 66-67 0.1682771551287108 68-69 0.17886746820384575 70-71 0.17225847583364876 72-73 0.1690999614077313 74-75 0.17430222046218358 76-77 0.17804466192482524 78-79 0.17992915372516252 80-81 0.17162146452085866 82-83 0.16947155134019218 84-85 0.16403041304344365 86-87 0.1611373199978554 88-89 0.16681733753690023 90-91 0.17467381039464444 92-93 0.16334031745458774 94-95 0.1698962255487189 96-97 0.16756051740182196 98-99 0.17342632990709722 100-101 0.14908718933090986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1883797.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.2344829644665 #Duplication Level Percentage of deduplicated Percentage of total 1 75.71353328480032 39.54857264544469 2 13.380527033675616 13.978498227922248 3 4.49765257129039 7.047976698454704 4 2.045885412530646 4.274630669123301 5 1.106979742860606 2.891125726023092 6 0.6976381616227993 2.18644612011887 7 0.4652593363618115 1.7011806615475022 8 0.32793074850789833 1.370343447716845 9 0.2287135618992223 1.075206118749064 >10 1.3516829623009219 13.176565116231604 >50 0.11567140803859637 4.178897484133219 >100 0.06395146399296749 5.8369073792184505 >500 0.0037611010749481516 1.2874978256256973 >1k 6.099082824240245E-4 0.6850584355915023 >5k 2.0330276080800819E-4 0.7610934440992555 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8215 0.4360873278808704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6119 0.3248226852468711 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4147 0.22014049284503584 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2551 0.13541798824395623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2290 0.12156299219077216 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.6542138032919683E-5 0.0 22-23 0.0 0.0 0.0 7.962641409875905E-5 0.0 24-25 0.0 0.0 0.0 1.0616855213167873E-4 0.0 26-27 0.0 0.0 0.0 1.3271069016459843E-4 0.0 28-29 5.3084276065839366E-5 0.0 0.0 7.697220029546708E-4 0.0 30-31 7.962641409875905E-5 0.0 0.0 0.0023091660088640125 0.0 32-33 1.0616855213167873E-4 0.0 0.0 0.005441138296748535 0.0 34-35 1.3271069016459843E-4 0.0 0.0 0.008891616241028095 0.0 36-37 1.592528281975181E-4 0.0 0.0 0.013350695430558602 0.0 38-39 1.592528281975181E-4 0.0 0.0 0.018367159518780422 0.0 40-41 1.592528281975181E-4 0.0 0.0 0.023038575812574284 0.0 42-43 1.592528281975181E-4 0.0 0.0 0.028214292728993624 0.0 44-45 1.592528281975181E-4 0.0 0.0 0.03413318951033471 0.0 46-47 1.592528281975181E-4 0.0 0.0 0.044935839689733026 0.0 48-49 1.592528281975181E-4 0.0 0.0 0.05714522318487608 0.0 50-51 1.592528281975181E-4 0.0 0.0 0.06943423309411789 0.0 52-53 1.592528281975181E-4 0.0 0.0 0.08708475488600947 0.0 54-55 1.592528281975181E-4 0.0 0.0 0.11582989037566149 0.0 56-57 1.592528281975181E-4 0.0 0.0 0.1587219854368597 0.0 58-59 1.592528281975181E-4 0.0 0.0 0.20087090063313617 0.0 60-61 1.592528281975181E-4 0.0 0.0 0.2416661667897337 0.0 62-63 1.592528281975181E-4 0.0 0.0 0.2912734227732606 0.0 64-65 1.592528281975181E-4 0.0 0.0 0.3481532245778075 0.0 66-67 1.592528281975181E-4 0.0 0.0 0.42409028148999073 0.0 68-69 1.592528281975181E-4 0.0 0.0 0.5157933683937281 0.0 70-71 1.8579496623043779E-4 0.0 0.0 0.613362267802741 0.0 72-73 2.1233710426335747E-4 0.0 0.0 0.7135588388770128 0.0 74-75 2.1233710426335747E-4 0.0 0.0 0.8266548890352835 0.0 76-77 2.1233710426335747E-4 0.0 0.0 0.955039210700516 0.0 78-79 2.1233710426335747E-4 0.0 0.0 1.0893689712851224 0.0 80-81 3.185056563950362E-4 0.0 0.0 1.238562329168164 0.0 82-83 3.7158993246087557E-4 0.0 0.0 1.3950813171482914 0.0 84-85 3.7158993246087557E-4 0.0 0.0 1.5758332771524746 0.0 86-87 3.7158993246087557E-4 0.0 0.0 1.7755097815741292 0.0 88-89 3.7158993246087557E-4 0.0 0.0 1.9987822467070497 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10310 0.0 31.692837 1 GGTATCA 6965 0.0 30.183125 1 GTACATG 9580 0.0 27.604017 1 TACATGG 9755 0.0 26.609907 2 ATCAACG 12370 0.0 26.057817 3 TCAACGC 12475 0.0 25.80107 4 CAACGCA 12525 0.0 25.660109 5 ACATGGG 9865 0.0 25.49392 3 TATCAAC 12675 0.0 25.430782 2 AACGCAG 12730 0.0 25.339369 6 CGTATAG 105 0.002180061 22.648726 1 ACGCAGA 14340 0.0 22.296846 7 GAGTACT 6765 0.0 22.224768 12-13 CGCAGAG 14460 0.0 22.111221 8 AGTACTT 7320 0.0 21.415792 12-13 AGAGTAC 12060 0.0 20.896578 10-11 CATGGGG 3805 0.0 20.741634 4 CATGGGA 5735 0.0 20.725077 4 GCAGAGT 15260 0.0 20.546236 9 GTACTTT 7350 0.0 20.45477 14-15 >>END_MODULE