FastQCFastQC Report
Thu 26 May 2016
SRR937603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883797
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93840.4981428466018366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79080.4197904551286577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54530.28946855738702204No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24600.13058731912196483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.10038236604050224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69750.037.3432541
GTATCAA101200.031.3741341
ATCAACG127150.024.8896263
TCAACGC128200.024.6487054
GTACATG90000.024.4519941
CAACGCA129800.024.2716525
TATCAAC130650.024.2592242
AACGCAG132750.023.7579826
TACATGG91500.023.5254332
CGTCGAT853.1532527E-622.35203644-45
GAGTACT83450.022.17073812-13
ACATGGG94900.021.831363
GTACTTT86750.021.32622314-15
AGTACTT86600.020.73351712-13
ACGCAGA152300.020.6470097
CGCAGAG152700.020.5618178
CGTACAC1656.306792E-520.1592583
AGAGTAC134300.019.73591410-11
TAGCCCT9850.019.2967534
GCAGAGT162600.018.7548489
Produced by FastQC (version 0.11.5)