##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1883797 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92950036548524 31.0 31.0 34.0 30.0 34.0 2 32.21217148132203 33.0 31.0 34.0 30.0 34.0 3 32.31145500284797 34.0 31.0 34.0 30.0 34.0 4 36.00739410881321 37.0 35.0 37.0 35.0 37.0 5 35.90698307726363 37.0 35.0 37.0 35.0 37.0 6 35.94856027480668 37.0 35.0 37.0 35.0 37.0 7 35.887479383394286 37.0 35.0 37.0 35.0 37.0 8 35.855182909835825 37.0 35.0 37.0 35.0 37.0 9 37.51616177326962 39.0 37.0 39.0 35.0 39.0 10-11 37.41453882769746 39.0 37.0 39.0 34.0 39.0 12-13 37.29565659144802 39.0 37.0 39.0 34.0 39.0 14-15 38.44798749546793 40.0 38.0 41.0 34.0 41.0 16-17 38.39978776906429 40.0 38.0 41.0 34.0 41.0 18-19 38.37166531213289 40.0 38.0 41.0 34.0 41.0 20-21 38.26820671229437 40.0 38.0 41.0 34.0 41.0 22-23 38.20805638824141 40.0 38.0 41.0 33.5 41.0 24-25 38.22882216077422 40.0 38.0 41.0 33.5 41.0 26-27 38.080851333768976 40.0 37.5 41.0 33.0 41.0 28-29 37.92193452903896 40.0 37.0 41.0 33.0 41.0 30-31 37.963987627116936 40.0 37.0 41.0 33.0 41.0 32-33 37.88063416599559 40.0 37.0 41.0 33.0 41.0 34-35 37.718949812532884 40.0 37.0 41.0 33.0 41.0 36-37 37.55050836156974 40.0 37.0 41.0 33.0 41.0 38-39 37.39969832205912 40.0 36.0 41.0 32.5 41.0 40-41 37.21000431575165 39.5 36.0 41.0 32.0 41.0 42-43 36.971636540455265 39.0 36.0 41.0 31.0 41.0 44-45 36.64904259853901 39.0 35.5 41.0 30.5 41.0 46-47 36.268444529851145 39.0 35.0 40.0 30.0 41.0 48-49 35.92561326937032 39.0 35.0 40.0 29.0 41.0 50-51 35.652850068239836 39.0 35.0 40.0 28.0 41.0 52-53 35.39534965816381 38.0 35.0 40.0 27.5 41.0 54-55 35.1410117438344 38.0 34.0 40.0 27.0 41.0 56-57 34.84153069571721 38.0 34.0 40.0 26.0 41.0 58-59 34.55199286334992 38.0 34.0 40.0 26.0 41.0 60-61 34.18687231161319 37.0 33.5 40.0 25.5 41.0 62-63 33.77904094761803 37.0 33.0 39.5 24.5 41.0 64-65 33.334334325832344 36.0 32.5 39.0 23.0 40.0 66-67 32.87930042355944 36.0 32.0 39.0 22.0 40.0 68-69 32.401576709167706 35.0 31.0 38.0 21.5 40.0 70-71 31.985138791493988 35.0 31.0 38.0 20.5 40.0 72-73 31.45985740501763 35.0 30.5 37.0 18.5 39.0 74-75 30.946105392460016 34.0 30.0 36.5 18.0 39.0 76-77 29.772498310592916 33.5 28.5 35.0 17.5 37.5 78-79 30.38762536515346 34.0 30.0 36.0 17.5 37.5 80-81 30.189550678762096 34.0 30.0 35.0 14.0 37.0 82-83 29.787778354037087 34.0 29.5 35.0 8.5 37.0 84-85 29.35729035559564 34.0 29.0 35.0 7.0 36.0 86-87 28.910879993969626 34.0 29.0 35.0 2.0 36.0 88-89 28.400774074913592 33.0 28.5 35.0 2.0 35.5 90-91 27.763760373331095 33.0 26.5 35.0 2.0 35.0 92-93 27.216602425845245 32.5 26.0 34.0 2.0 35.0 94-95 26.624484750745438 32.0 25.0 34.0 2.0 35.0 96-97 25.89907856313605 32.0 24.5 34.0 2.0 35.0 98-99 24.801813040364756 31.5 19.0 34.0 2.0 35.0 100-101 21.844950650202755 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2843297506986495 1101 2 0.2639802894918404 1101 3 0.2859414956568429 1101 4 0.1015479231123777 1101 5 0.019552425515854566 1101 6 0.13034787041696916 1101 7 0.25607889960173225 1101 8 0.09422635102452404 1101 9 0.15458824825873307 1101 10-11 0.2250455556144786 1101 12-13 0.31046048824629224 1101 14-15 0.330242962633605 1101 16-17 0.32597651421664153 1101 18-19 0.35837345423372113 1101 20-21 0.3519921679619529 1101 22-23 0.34396760090319844 1101 24-25 0.29859281797605064 1101 26-27 0.10406123289909885 1101 28-29 0.019878678357827084 1101 30-31 0.032199903832854204 1101 32-33 0.2540322439897622 1101 34-35 0.2616027207232321 1101 36-37 0.14975710957733668 1101 38-39 0.20518190859746 1101 40-41 0.18571067632259997 1101 42-43 0.19145121189330894 1101 44-45 0.2984090997166007 1101 46-47 0.2688081617493552 1101 48-49 0.1671020909310954 1101 50-51 0.17733498880249243 1101 52-53 0.2547066097963011 1101 54-55 0.031288447476484293 1101 56-57 0.06423811118316536 1101 58-59 -0.03575535654199058 1101 60-61 0.06989598953158094 1101 62-63 -0.07753126610581518 1101 64-65 -0.07787457147389176 1101 66-67 0.08098261852887845 1101 68-69 -0.03939868863572826 1101 70-71 -0.1571235790763339 1101 72-73 -0.10038306146470433 1101 74-75 -0.20434687712804944 1101 76-77 -0.07719101634312864 1101 78-79 -0.11811457586800245 1101 80-81 -0.05178574242343714 1101 82-83 -0.10027667699847242 1101 84-85 -0.2506392882334616 1101 86-87 -0.48309386227899154 1101 88-89 -0.352983465149169 1101 90-91 -0.37428093217226177 1101 92-93 -0.4834147283889969 1101 94-95 -0.624475360473344 1101 96-97 -0.673789724359299 1101 98-99 -0.7014969189799629 1101 100-101 -0.664027286631633 1102 1 0.2978309964355965 1102 2 0.2130862234950257 1102 3 0.1532605531219744 1102 4 0.07504602065107946 1102 5 0.040207537530214665 1102 6 0.07318736042159912 1102 7 0.004123304491891133 1102 8 0.14929369155856165 1102 9 0.20670112582345723 1102 10-11 0.15839345095791657 1102 12-13 0.2643240890522094 1102 14-15 0.18103174880243955 1102 16-17 0.19369578797701337 1102 18-19 0.27164967389766304 1102 20-21 0.23736062983450523 1102 22-23 0.2872353478352139 1102 24-25 0.09630658238949508 1102 26-27 0.20269335987983084 1102 28-29 0.05914926504113538 1102 30-31 0.10227710797789058 1102 32-33 0.27634143465746064 1102 34-35 0.07804826470034243 1102 36-37 -0.15687835551319296 1102 38-39 0.0535268580812982 1102 40-41 0.06533622554458418 1102 42-43 0.06516687924601428 1102 44-45 0.09062482904547409 1102 46-47 -0.07704356129969625 1102 48-49 0.011133244073413096 1102 50-51 -0.028916128441444755 1102 52-53 -0.0309050726957949 1102 54-55 0.14473032992059842 1102 56-57 0.11459542212477913 1102 58-59 -0.010808434590849458 1102 60-61 -0.23440413700176777 1102 62-63 -0.2787787237286139 1102 64-65 -0.23131689364610253 1102 66-67 -0.1393136689870147 1102 68-69 0.009857071563331488 1102 70-71 -0.2966810086807996 1102 72-73 -0.37424396654621006 1102 74-75 -0.36215668707947657 1102 76-77 -0.20793444678945505 1102 78-79 -0.05039979498164726 1102 80-81 -0.13983157483668762 1102 82-83 0.009338933731275034 1102 84-85 -0.11596172523205794 1102 86-87 -0.30031578125876734 1102 88-89 -0.3452354879780657 1102 90-91 -0.3248597959722197 1102 92-93 -0.29418411553867685 1102 94-95 -0.3537174062541375 1102 96-97 -0.36129153664447244 1102 98-99 -0.17007213303673296 1102 100-101 0.17779439215464166 1103 1 0.4374942864005895 1103 2 0.31935051391416636 1103 3 0.27903348760896307 1103 4 0.15467389261497289 1103 5 0.15675049131162666 1103 6 0.20196565117779386 1103 7 0.26033892768501943 1103 8 0.27376832908076665 1103 9 0.3096677843108395 1103 10-11 0.2646258210018999 1103 12-13 0.37617656306282043 1103 14-15 0.35306895555478945 1103 16-17 0.3571105601215123 1103 18-19 0.31798050997851846 1103 20-21 0.14843409540996788 1103 22-23 0.18793484276714167 1103 24-25 0.24969502854495573 1103 26-27 0.18272708958050998 1103 28-29 0.14718944174990867 1103 30-31 0.21541169184361308 1103 32-33 0.20681099094003486 1103 34-35 0.281175463008168 1103 36-37 0.2583052743871548 1103 38-39 0.16864701030669238 1103 40-41 0.0520795878462863 1103 42-43 0.1283167274048722 1103 44-45 0.07888630018447174 1103 46-47 -0.024641953965094388 1103 48-49 -0.14785034461787916 1103 50-51 -0.12390299570321162 1103 52-53 -0.1058949595342824 1103 54-55 -0.35273646305595463 1103 56-57 -0.004809170596949741 1103 58-59 -0.14799297883597262 1103 60-61 -0.08690159623068183 1103 62-63 -0.10823829513631011 1103 64-65 -0.19140069143853822 1103 66-67 -0.19906059336053517 1103 68-69 -0.32567523775629326 1103 70-71 -0.3214106763851561 1103 72-73 -0.4861041559081585 1103 74-75 -0.4443696555866232 1103 76-77 -0.26283703820956106 1103 78-79 -0.17386190610146102 1103 80-81 -0.19967713468224702 1103 82-83 -0.12132583606835823 1103 84-85 -0.16453303882962445 1103 86-87 -0.1860382664248732 1103 88-89 -0.034824362622970284 1103 90-91 0.03662877063350223 1103 92-93 0.0019210843358195007 1103 94-95 -0.17888618334674433 1103 96-97 -0.38165234883194543 1103 98-99 -0.0764649521323193 1103 100-101 0.07506191727264877 1104 1 0.31704209391216054 1104 2 0.21869936531727774 1104 3 0.16342219492220522 1104 4 0.15091094006060501 1104 5 0.1716569701052748 1104 6 0.15206098685131764 1104 7 0.1317977796015839 1104 8 0.1621729410473165 1104 9 0.13907770101940287 1104 10-11 0.20464440950018314 1104 12-13 0.16748382937294082 1104 14-15 0.2226847671495733 1104 16-17 0.22038008783499663 1104 18-19 0.20131476674172433 1104 20-21 0.17607984527805343 1104 22-23 0.19115200475481942 1104 24-25 0.09561471103206287 1104 26-27 0.17645941810610566 1104 28-29 0.1633155731811584 1104 30-31 0.3072511438426133 1104 32-33 0.17629849896444938 1104 34-35 0.09023593451436085 1104 36-37 0.04347178039635935 1104 38-39 -0.007372405507389601 1104 40-41 -0.07601831389864344 1104 42-43 0.005702548685512454 1104 44-45 -0.11810855926032104 1104 46-47 -0.3474722152744718 1104 48-49 -0.29307153701434885 1104 50-51 -0.4091679847830534 1104 52-53 -0.29262437301900945 1104 54-55 -0.5252745966001626 1104 56-57 -0.40468626256447493 1104 58-59 -0.5838290101799899 1104 60-61 -0.4310738206398028 1104 62-63 -0.318079321123939 1104 64-65 -0.19092321534352408 1104 66-67 -0.24209813034971006 1104 68-69 -0.12872998245017442 1104 70-71 -0.24313316994749812 1104 72-73 -0.10596515989012545 1104 74-75 -0.12594255931137255 1104 76-77 -0.27566681508086077 1104 78-79 -0.28062567697907426 1104 80-81 -0.38212028645379803 1104 82-83 -0.47874918244477627 1104 84-85 -0.29330436752143996 1104 86-87 -0.31176068108472066 1104 88-89 -0.3985617937029531 1104 90-91 -0.35534603394505027 1104 92-93 -0.09971649042211084 1104 94-95 -0.14229747446705332 1104 96-97 -0.29826590619419946 1104 98-99 -0.023092784463369043 1104 100-101 0.07906665374268584 1105 1 0.30163421937297485 1105 2 0.23188624931956525 1105 3 0.1616237522158741 1105 4 0.10611889153459231 1105 5 0.1052290594502594 1105 6 0.0852794926939282 1105 7 0.14429982521357232 1105 8 0.09024512521825301 1105 9 0.19638988416152614 1105 10-11 0.21405210484517312 1105 12-13 0.2846666591771765 1105 14-15 0.27617776797686133 1105 16-17 0.1646470182242652 1105 18-19 0.25057776701221 1105 20-21 0.0657113411736816 1105 22-23 0.029809393646644367 1105 24-25 0.11391074945536417 1105 26-27 0.08761513305844915 1105 28-29 0.04898571849521005 1105 30-31 0.21858774883614274 1105 32-33 0.1880607325720618 1105 34-35 0.2530590175533476 1105 36-37 0.2763672844591838 1105 38-39 0.3761411913469246 1105 40-41 0.2791428888053247 1105 42-43 0.26950071383868845 1105 44-45 0.36913035493589774 1105 46-47 0.35858613137359185 1105 48-49 0.38360941902343626 1105 50-51 0.4105632182685639 1105 52-53 0.27240969507647605 1105 54-55 0.3407670007606569 1105 56-57 0.5533380620585362 1105 58-59 0.3668467978402816 1105 60-61 0.3830749461564764 1105 62-63 0.3983416224380889 1105 64-65 0.6344500611488755 1105 66-67 0.32085301718562675 1105 68-69 0.4426644903980872 1105 70-71 0.2954747002955571 1105 72-73 0.2939321284833625 1105 74-75 0.10386650842375289 1105 76-77 0.1483418438992956 1105 78-79 0.3310997850748585 1105 80-81 0.3128829935914048 1105 82-83 0.2976495286006262 1105 84-85 0.21393462581417566 1105 86-87 -0.01693690614132848 1105 88-89 0.10340213512699137 1105 90-91 0.2987977188053996 1105 92-93 0.3958953715553939 1105 94-95 0.18602628818147338 1105 96-97 0.10472502584456578 1105 98-99 0.1255698587188725 1105 100-101 0.15554343014647998 1106 1 0.1307844831715279 1106 2 0.11085322499319261 1106 3 0.006349005958000475 1106 4 -0.05241522844778501 1106 5 -0.08408377166288972 1106 6 -0.09630604919171049 1106 7 0.02333025301965108 1106 8 -0.11740065015352741 1106 9 -0.0934323052557815 1106 10-11 -0.13030468383945504 1106 12-13 -0.367305624931376 1106 14-15 -0.035547359746381346 1106 16-17 -0.12727661728189332 1106 18-19 -0.05562377879979152 1106 20-21 0.16338084764299765 1106 22-23 0.21296972892181287 1106 24-25 0.09999215529490613 1106 26-27 0.09727077686002161 1106 28-29 0.02353388766508857 1106 30-31 0.03941191235258401 1106 32-33 0.0918305565676576 1106 34-35 -0.25373537776405897 1106 36-37 0.037668330593007227 1106 38-39 0.022012331575844257 1106 40-41 -0.1447502625256547 1106 42-43 -0.24484109128889742 1106 44-45 -0.1145215720463355 1106 46-47 -0.053082007480924176 1106 48-49 -0.22941594618784933 1106 50-51 0.03167592666823538 1106 52-53 0.1616632943618015 1106 54-55 -0.10232853660659913 1106 56-57 -0.18223786638635175 1106 58-59 -0.10350998907358644 1106 60-61 0.013205235518860547 1106 62-63 -0.27103661454294325 1106 64-65 -0.700258609452213 1106 66-67 -0.6837732335223095 1106 68-69 -0.02308877052504954 1106 70-71 -0.2782290833108334 1106 72-73 -0.12289983344771827 1106 74-75 -0.17569901269090238 1106 76-77 -0.13967520613138262 1106 78-79 -0.10246258586276724 1106 80-81 -0.37030109934265454 1106 82-83 -0.4052715714763728 1106 84-85 -0.47597878711075836 1106 86-87 -0.08676833891113844 1106 88-89 -0.21485221702526047 1106 90-91 -0.43590271777478407 1106 92-93 -0.323538039268243 1106 94-95 -0.29736371430053765 1106 96-97 -0.1679599231030302 1106 98-99 -0.06914953676823643 1106 100-101 -0.20455996436552581 1107 1 0.26825371224576955 1107 2 0.18253605588634514 1107 3 0.15399123334659492 1107 4 0.09347381662236387 1107 5 0.1244698252716745 1107 6 0.11483613322482711 1107 7 0.13120874523046666 1107 8 0.09094103963712286 1107 9 0.13224094382505314 1107 10-11 0.1644332659065313 1107 12-13 0.1805177132057736 1107 14-15 0.21262908904500932 1107 16-17 0.23760885534817078 1107 18-19 0.17811955216511421 1107 20-21 0.02916357820044624 1107 22-23 0.05017235668682929 1107 24-25 0.10140302607317864 1107 26-27 0.15664829380312995 1107 28-29 0.0997274590194408 1107 30-31 0.21859366551743875 1107 32-33 0.2443258359576177 1107 34-35 0.13241168754030497 1107 36-37 0.18966353240982414 1107 38-39 0.1703206983859431 1107 40-41 0.32869861647682086 1107 42-43 0.28305104098890155 1107 44-45 0.10214067503681434 1107 46-47 0.10083534931511196 1107 48-49 0.10346456176055341 1107 50-51 0.2616727509718899 1107 52-53 0.2233726631317552 1107 54-55 0.2782524731949749 1107 56-57 0.28762314523157073 1107 58-59 0.3342446160499648 1107 60-61 0.4452668737182677 1107 62-63 0.3206517934668014 1107 64-65 0.3338622247749825 1107 66-67 0.2695863825066027 1107 68-69 0.38435714585254743 1107 70-71 0.40943899791363947 1107 72-73 0.4000114621636186 1107 74-75 0.3906932710946798 1107 76-77 0.27338639510070806 1107 78-79 0.2392681016973235 1107 80-81 0.29294279151701375 1107 82-83 0.20391154685950497 1107 84-85 0.4618547010292815 1107 86-87 0.2451003706413033 1107 88-89 0.05423085086623658 1107 90-91 0.14912371385736378 1107 92-93 0.26918865553045634 1107 94-95 0.2623213299743732 1107 96-97 0.31388548539601757 1107 98-99 0.5492876364021093 1107 100-101 0.7234649591747093 1108 1 0.1922738360839169 1108 2 0.2207881793378803 1108 3 0.19483826984646413 1108 4 0.13504269610763942 1108 5 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-0.7121225519099923 2316 78-79 -0.7297540500787143 2316 80-81 -0.9870614503179027 2316 82-83 -0.863210997234912 2316 84-85 -0.8878039622124199 2316 86-87 -0.9365214173948218 2316 88-89 -1.076795372763197 2316 90-91 -0.8909349220640443 2316 92-93 -0.853270961661913 2316 94-95 -0.9912158647810543 2316 96-97 -1.0579920991511749 2316 98-99 -1.03076313209667 2316 100-101 -0.6041107423007688 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 15.0 5 37.0 6 103.0 7 236.0 8 570.0 9 1042.0 10 1703.0 11 2851.0 12 4308.0 13 6037.0 14 8078.0 15 11205.0 16 14642.0 17 19911.0 18 17882.0 19 11611.0 20 10691.0 21 10870.0 22 11600.0 23 13052.0 24 15333.0 25 18346.0 26 22487.0 27 27947.0 28 34667.0 29 42910.0 30 53409.0 31 66592.0 32 84323.0 33 109541.0 34 147331.0 35 206421.0 36 296454.0 37 382870.0 38 217512.0 39 11200.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59785671962571 19.815308781460583 15.299543183106906 22.287291315806797 2 15.75737725455556 20.696975311034045 39.69116629870416 23.854481135706234 3 19.644738790856977 24.48814813910416 30.181065157232972 25.686047912805893 4 13.103906631128515 17.052845927666304 36.763196883740655 33.080050557464524 5 13.526495649333924 37.994949669285646 33.85782071060437 14.620733970776056 6 32.15027946217135 36.467039707569334 16.80833975210705 14.574341078152264 7 28.80984522217627 33.31654100733784 19.870612385517123 18.003001384968762 8 26.944463761222682 35.16790821941005 18.964835383005706 18.92279263636156 9 26.440216222873268 16.839500222157696 20.311211876863588 36.40907167810544 10-11 25.380017061286324 27.042802382634644 26.50067921331226 21.07650134276676 12-13 26.041894494545932 24.244398884767048 27.693456394009342 22.02025022667768 14-15 22.631339788735197 25.56257388667675 26.35984662890959 25.446239695678464 16-17 21.90044893372269 29.16670426802888 26.870676617491164 22.062170180757267 18-19 21.7841943691385 27.12264113383767 29.843024487245707 21.250140009778125 20-21 22.553677492850873 26.041765646723082 30.04394847215491 21.360608388271135 22-23 22.250811198184508 26.13405174415904 29.993191907261302 21.621945150395145 24-25 22.0984736645693 26.309525515807735 30.179802133249247 21.412198686373713 26-27 21.90382255663285 26.692097823602936 29.96143124045635 21.44264837930786 28-29 21.555551957591685 26.84831016760702 30.146596088771442 21.449541786029855 30-31 21.70446178648761 26.416938767818397 30.120628708932013 21.757970736761976 32-33 21.603230072030055 26.503970438428343 30.04004677786407 21.85275271167753 34-35 22.24616028160147 26.518435903656286 29.80039781356484 21.43500600117741 36-37 22.245868318083104 26.216625252083958 29.783994772260492 21.753511657572446 38-39 21.816283814020302 26.602309059840312 29.96413626308992 21.617270863049466 40-41 21.95038246046068 26.58057070601572 29.809912413249997 21.659134420273602 42-43 21.992124197189717 26.50612451838019 29.51180374996516 21.989947534464932 44-45 22.00255653873533 26.701337776841132 29.35921439518165 21.93689128924189 46-47 22.260333783311047 27.029159723685726 28.85297619647977 21.857530296523457 48-49 22.336960935812087 27.151757859259785 28.56655998496653 21.9447212199616 50-51 22.18301653522115 27.232313248189694 28.562605206399628 22.022065010189525 52-53 22.5886865729163 27.353159602653577 28.19274051291089 21.865413311519237 54-55 22.199788193513626 27.18671617665404 28.38193646336252 22.23155916646981 56-57 22.32934334219664 27.509333542839276 28.221591816952674 21.93973129801141 58-59 22.640735705598853 27.068601340802644 28.189210408552512 22.10145254504599 60-61 22.355115758226603 27.137823236792503 28.759494786327828 21.747566218653073 62-63 22.28881349742037 27.188067504088814 28.633366546395393 21.889752452095422 64-65 22.172320053593886 27.124552167776038 28.507636438533453 22.195491340096623 66-67 22.098851415518762 27.37216377348515 28.255937343567272 22.273047467428817 68-69 22.1692677077201 27.082987179616484 28.512785613311838 22.234959499351575 70-71 22.207408760073406 27.193429015971464 28.44443960787707 22.15472261607806 72-73 22.354160241257418 27.11643027353797 28.331768231927324 22.19764125327729 74-75 22.153767099108872 27.557295186264763 28.01761548616969 22.271322228456675 76-77 22.188537299931998 27.27873544760927 28.27154412073063 22.2611831317281 78-79 22.279099074900323 27.385912600986202 28.124686470994487 22.210301853118995 80-81 22.03592000624271 27.64793658764718 28.081980170899516 22.234163235210588 82-83 22.247074583360206 27.643690928640456 28.01668868343445 22.09254580456489 84-85 22.14194105943531 27.508233375588443 27.953823025423137 22.396002539553113 86-87 22.15055550040689 27.33829600535514 28.174930738290804 22.336217755947164 88-89 22.155863928013474 27.49754883355266 27.85284189326132 22.49374534517254 90-91 22.093048874722175 27.480255245071746 28.018506037503155 22.408189842702928 92-93 22.320925210456075 27.324519075086823 28.074028302878386 22.28052741157872 94-95 22.07610480322455 27.171053993609718 28.10432865112324 22.648512552042497 96-97 22.18036232141786 27.04872127941599 28.151228609027406 22.619687790138745 98-99 22.09532009858906 27.19822760525518 27.75917451038577 22.947277785769987 100-101 22.026574157253258 26.93290731431751 28.33522875692068 22.705289771508543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 384.0 1 283.0 2 266.5 3 394.0 4 328.5 5 271.0 6 574.5 7 1383.0 8 2337.0 9 2789.0 10 2443.5 11 2037.0 12 2158.5 13 2398.0 14 2610.5 15 2861.5 16 3033.0 17 3079.0 18 3235.0 19 3383.0 20 3692.0 21 4299.0 22 5136.0 23 6141.5 24 7233.5 25 8876.0 26 11331.0 27 14099.5 28 16097.0 29 18493.5 30 22324.5 31 25761.5 32 29354.0 33 33369.0 34 37471.0 35 41335.5 36 45627.5 37 50301.0 38 53021.0 39 54063.0 40 55458.0 41 58998.0 42 62232.0 43 65487.5 44 68771.0 45 71338.5 46 71712.5 47 72738.5 48 78232.0 49 77686.5 50 72172.0 51 67754.0 52 63182.5 53 61378.0 54 60261.5 55 55323.0 56 49009.5 57 43872.0 58 39947.0 59 35449.5 60 29554.5 61 23727.5 62 18723.5 63 14106.5 64 10379.5 65 8145.0 66 6243.0 67 4887.0 68 3466.0 69 2194.5 70 1538.5 71 1171.5 72 1012.5 73 881.5 74 775.0 75 655.5 76 437.5 77 281.0 78 190.0 79 117.5 80 76.5 81 55.5 82 38.5 83 23.5 84 14.5 85 6.0 86 3.0 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01746472682566115 2 0.0 3 0.0 4 0.0 5 0.03991937560151121 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0034504779442795586 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.043006226254739E-4 24-25 2.1233710426335747E-4 26-27 9.820591072180283E-4 28-29 8.228062790205102E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.9813207049379525E-4 42-43 0.009528627553818166 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.0616855213167873E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.919635183621165E-4 84-85 5.3084276065839366E-5 86-87 0.0 88-89 0.0 90-91 0.010377975970871596 92-93 0.0016456125580410204 94-95 0.0 96-97 0.0 98-99 9.555169691851087E-4 100-101 0.016615378408607724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1883797.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.56654902543723 #Duplication Level Percentage of deduplicated Percentage of total 1 74.44045694304569 37.64197015486469 2 13.987945248479338 14.146442383447244 3 4.748802592621406 7.203916773357412 4 2.2132846568384905 4.476726684290862 5 1.1717581871024012 2.9625883907035506 6 0.7288132266460735 2.211214185335146 7 0.4757117710352787 1.6838571814423096 8 0.34704987996407666 1.4039291815580472 9 0.2539809613470116 1.1558646660133207 >10 1.4307632501068142 13.520661793636046 >50 0.12943565403053542 4.531800835316325 >100 0.06747212571566674 6.155144075270244 >500 0.0037887932656164537 1.3300339779785324 >1k 4.209770295129393E-4 0.36539745889887176 >5k 3.157327721347045E-4 1.2104522578873929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9384 0.4981428466018366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7908 0.4197904551286577 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5453 0.28946855738702204 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2460 0.13058731912196483 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1891 0.10038236604050224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.3084276065839366E-5 0.0 0.0 0.0 0.0 7 5.3084276065839366E-5 0.0 0.0 0.0 0.0 8 5.3084276065839366E-5 0.0 0.0 0.0 0.0 9 5.3084276065839366E-5 0.0 0.0 0.0 0.0 10-11 5.3084276065839366E-5 0.0 0.0 0.0 0.0 12-13 5.3084276065839366E-5 0.0 0.0 0.0 0.0 14-15 5.3084276065839366E-5 0.0 0.0 0.0 0.0 16-17 5.3084276065839366E-5 0.0 0.0 0.0 0.0 18-19 7.962641409875905E-5 0.0 0.0 0.0 0.0 20-21 1.0616855213167873E-4 0.0 0.0 2.6542138032919683E-5 0.0 22-23 1.0616855213167873E-4 0.0 0.0 7.962641409875905E-5 0.0 24-25 1.0616855213167873E-4 0.0 0.0 1.0616855213167873E-4 0.0 26-27 1.0616855213167873E-4 0.0 0.0 1.3271069016459843E-4 0.0 28-29 1.592528281975181E-4 0.0 0.0 7.697220029546708E-4 0.0 30-31 2.1233710426335747E-4 0.0 0.0 0.0022826238708310927 0.0 32-33 2.1233710426335747E-4 0.0 0.0 0.005467680434781455 0.0 34-35 2.3887924229627717E-4 0.0 0.0 0.008811989826929335 0.0 36-37 3.185056563950362E-4 0.0 0.0 0.013138358326295244 0.0 38-39 3.7158993246087557E-4 0.0 0.0 0.018075196000418306 0.0 40-41 3.7158993246087557E-4 0.0 0.0 0.02266698588011341 0.0 42-43 4.2467420852671493E-4 0.0 0.0 0.027948871348664424 0.0 44-45 4.777584845925543E-4 0.0 0.0 0.0339739366821372 0.0 46-47 5.308427606583937E-4 0.0 0.0 0.04514817679399638 0.0 48-49 5.308427606583937E-4 0.0 0.0 0.057331018151106514 0.0 50-51 5.308427606583937E-4 0.0 0.0 0.06962002806034834 0.0 52-53 5.308427606583937E-4 0.0 0.0 0.0871112970240424 0.0 54-55 5.308427606583937E-4 0.0 0.0 0.11596260106582609 0.0 56-57 5.308427606583937E-4 0.0 0.0 0.1592528281975181 0.0 58-59 5.308427606583937E-4 0.0 0.0 0.2014548276698604 0.0 60-61 5.308427606583937E-4 0.0 0.0 0.2424624309307213 0.0 62-63 5.308427606583937E-4 0.0 0.0 0.2918308076719519 0.0 64-65 5.308427606583937E-4 0.0 0.0 0.3481266824397745 0.0 66-67 5.839270367242331E-4 0.0 0.0 0.4243557028703199 0.0 68-69 5.839270367242331E-4 0.0 0.0 0.5156075734274977 0.0 70-71 5.839270367242331E-4 0.0 0.0 0.6132030149745434 0.0 72-73 5.839270367242331E-4 0.0 0.0 0.7135322967389799 0.0 74-75 5.839270367242331E-4 0.0 0.0 0.8277431166946332 0.0 76-77 5.839270367242331E-4 0.0 0.0 0.9564459440162607 0.0 78-79 5.839270367242331E-4 0.0 0.0 1.0918904743982498 0.0 80-81 7.697220029546708E-4 0.0 0.0 1.242278228492773 0.0 82-83 7.962641409875905E-4 0.0 0.0 1.4016903095184885 0.0 84-85 7.962641409875905E-4 0.0 0.0 1.5847779776695683 0.0 86-87 8.228062790205102E-4 0.0 0.0 1.7875333701030418 0.0 88-89 8.493484170534299E-4 0.0 0.0 2.0140970603520443 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6975 0.0 37.343254 1 GTATCAA 10120 0.0 31.374134 1 ATCAACG 12715 0.0 24.889626 3 TCAACGC 12820 0.0 24.648705 4 GTACATG 9000 0.0 24.451994 1 CAACGCA 12980 0.0 24.271652 5 TATCAAC 13065 0.0 24.259224 2 AACGCAG 13275 0.0 23.757982 6 TACATGG 9150 0.0 23.525433 2 CGTCGAT 85 3.1532527E-6 22.352036 44-45 GAGTACT 8345 0.0 22.170738 12-13 ACATGGG 9490 0.0 21.83136 3 GTACTTT 8675 0.0 21.326223 14-15 AGTACTT 8660 0.0 20.733517 12-13 ACGCAGA 15230 0.0 20.647009 7 CGCAGAG 15270 0.0 20.561817 8 CGTACAC 165 6.306792E-5 20.159258 3 AGAGTAC 13430 0.0 19.735914 10-11 TAGCCCT 985 0.0 19.296753 4 GCAGAGT 16260 0.0 18.754848 9 >>END_MODULE