FastQCFastQC Report
Thu 26 May 2016
SRR937602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894473
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80950.4272956120250856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62860.3318073152797638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41390.2184776452343211No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24860.13122382847367053No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22290.11765805055020577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100550.033.4312481
GGTATCA65500.031.9393881
GTACATG95100.027.04781
ATCAACG123450.026.9907933
TCAACGC125200.026.5797824
TATCAAC126300.026.4577062
CAACGCA126100.026.3900785
AACGCAG127550.026.1493826
TACATGG97950.026.1082692
ACATGGG100650.024.132143
ACGCAGA144900.022.8543667
CGCAGAG145450.022.8006118
GAGTACT71400.022.4213512-13
AGTACTT74000.021.44098912-13
GTACTTT74650.021.38071814-15
GTGTACG1805.1283587E-621.1316151
GCAGAGT155200.020.9696969
CATGGGG39950.020.4677144
ACCGTCG1404.8449566E-420.3623548
ATACCGT2351.1297743E-720.2179416
Produced by FastQC (version 0.11.5)