##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937602_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1894473 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.586259081021478 31.0 31.0 34.0 30.0 34.0 2 31.706051234301043 33.0 31.0 34.0 30.0 34.0 3 31.753618024643266 33.0 31.0 34.0 30.0 34.0 4 35.36120493667632 37.0 35.0 37.0 33.0 37.0 5 35.34208457972217 37.0 35.0 37.0 33.0 37.0 6 35.44122824658889 37.0 35.0 37.0 33.0 37.0 7 35.36981102396287 37.0 35.0 37.0 33.0 37.0 8 35.360254276519115 37.0 35.0 37.0 33.0 37.0 9 36.94845321099852 39.0 37.0 39.0 33.0 39.0 10-11 36.826073794664794 39.0 37.0 39.0 33.0 39.0 12-13 36.69397346913891 39.0 37.0 39.0 32.0 39.0 14-15 37.79408204814743 40.0 37.5 41.0 32.5 41.0 16-17 37.73194022823233 40.0 37.0 41.0 33.0 41.0 18-19 37.68346395013283 40.0 37.0 41.0 32.0 41.0 20-21 37.51149924015808 40.0 37.0 41.0 32.0 41.0 22-23 37.34938212368294 39.5 37.0 41.0 32.0 41.0 24-25 37.18929169220147 39.0 36.5 41.0 31.5 41.0 26-27 37.300874174506575 39.0 37.0 41.0 32.0 41.0 28-29 37.23861622730965 39.5 37.0 41.0 31.0 41.0 30-31 37.03614197721477 39.0 36.5 41.0 31.0 41.0 32-33 36.796594620245315 39.0 36.0 41.0 30.5 41.0 34-35 36.52172398339802 39.0 35.0 40.0 30.0 41.0 36-37 36.518676697952415 39.0 35.0 40.5 30.0 41.0 38-39 36.45604714345362 39.0 35.0 41.0 30.0 41.0 40-41 36.252957154839365 39.0 35.0 41.0 30.0 41.0 42-43 35.99524590743705 39.0 35.0 40.0 29.0 41.0 44-45 35.748624287598716 39.0 35.0 40.0 28.5 41.0 46-47 35.376528723291386 38.5 35.0 40.0 26.5 41.0 48-49 35.030674757571106 38.0 34.5 40.0 25.5 41.0 50-51 34.13635665433078 37.5 33.5 39.5 24.0 40.5 52-53 34.25266551700658 38.0 33.5 39.5 24.0 40.5 54-55 34.540341034155674 38.0 34.0 40.0 24.0 41.0 56-57 34.25334565338224 38.0 34.0 40.0 23.0 41.0 58-59 34.0013328244847 38.0 33.5 40.0 22.5 41.0 60-61 33.67114601263782 37.5 33.0 40.0 22.0 41.0 62-63 33.2864247735386 37.0 32.5 40.0 20.5 41.0 64-65 32.897920952159254 36.0 32.0 39.0 19.0 41.0 66-67 32.46589552873015 36.0 31.5 39.0 18.0 40.5 68-69 31.966173706355278 35.0 31.0 39.0 14.0 40.0 70-71 31.50525502342868 35.0 31.0 38.0 9.5 40.0 72-73 31.0135829330901 35.0 30.0 37.5 7.5 39.5 74-75 30.4981039581984 34.5 30.0 37.0 6.0 39.0 76-77 30.01628421202097 34.0 29.0 36.0 2.0 39.0 78-79 29.455704304046563 34.0 29.0 36.0 2.0 38.0 80-81 28.906356543482012 34.0 28.5 35.0 2.0 37.0 82-83 28.365548097016955 34.0 27.0 35.0 2.0 37.0 84-85 27.85770924156744 33.0 26.0 35.0 2.0 36.0 86-87 27.29794196064024 33.0 26.0 35.0 2.0 36.0 88-89 26.765754117371955 32.5 25.0 35.0 2.0 35.0 90-91 26.21070292371546 32.0 24.5 35.0 2.0 35.0 92-93 25.59520589630995 32.0 21.5 34.0 2.0 35.0 94-95 24.880372800245766 31.5 19.0 34.0 2.0 35.0 96-97 24.023030415318665 31.0 6.5 34.0 2.0 35.0 98-99 22.990507650412543 31.0 2.0 34.0 2.0 35.0 100-101 20.429943841902208 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3682856786593227 1101 2 0.48341784817422706 1101 3 0.3724645553151831 1101 4 0.347667859021513 1101 5 0.30319477408435347 1101 6 0.2761715811286507 1101 7 0.42887530701091947 1101 8 0.40767099155561226 1101 9 0.3655049254960332 1101 10-11 0.25872148872998935 1101 12-13 0.440799107199048 1101 14-15 0.6238609908074721 1101 16-17 0.7242015940658177 1101 18-19 0.6293964315391065 1101 20-21 0.6107039762220197 1101 22-23 0.7030437346706719 1101 24-25 0.6767480664207639 1101 26-27 0.7091962440707462 1101 28-29 0.6162242246096099 1101 30-31 0.49784158580614957 1101 32-33 0.5930694103742269 1101 34-35 0.6650745448948214 1101 36-37 0.5524744580876657 1101 38-39 0.5593768537456256 1101 40-41 0.5870690110102288 1101 42-43 0.565982298231404 1101 44-45 0.7170099627158564 1101 46-47 0.8696443079445686 1101 48-49 1.0639701487440192 1101 50-51 0.8925337509047822 1101 52-53 0.9213660250177611 1101 54-55 1.0038939800863744 1101 56-57 1.2212183359805522 1101 58-59 1.1398498037794624 1101 60-61 1.1200046166952546 1101 62-63 1.2576155174100663 1101 64-65 1.183680942947369 1101 66-67 1.1875742376419254 1101 68-69 1.2925707332258192 1101 70-71 1.451903067899238 1101 72-73 1.4108464439383361 1101 74-75 1.326747773016539 1101 76-77 1.3737708244012659 1101 78-79 1.4142630276557568 1101 80-81 1.4964960587226628 1101 82-83 1.5035563832550736 1101 84-85 1.571285943167748 1101 86-87 1.54621498173627 1101 88-89 1.633068661511487 1101 90-91 1.6595828438320837 1101 92-93 1.9290623118107142 1101 94-95 1.7347246538359755 1101 96-97 2.062470432185446 1101 98-99 2.421656218221692 1101 100-101 2.8908735620965125 1102 1 0.2748088493659324 1102 2 0.3468099152340258 1102 3 0.3300580007044829 1102 4 0.17493479717732185 1102 5 0.20657137266532288 1102 6 0.22484206937466666 1102 7 0.19860876442744058 1102 8 0.22600125329645238 1102 9 0.20570342049192192 1102 10-11 0.15583795505718 1102 12-13 0.26522172593362114 1102 14-15 0.4622341081232406 1102 16-17 0.5569776772594821 1102 18-19 0.4346282186343018 1102 20-21 0.47053875045997984 1102 22-23 0.5317400494456379 1102 24-25 0.5679589579219027 1102 26-27 0.6745256437840155 1102 28-29 0.5882549169069193 1102 30-31 0.5264009651005708 1102 32-33 0.42582920193274987 1102 34-35 0.6382515109236166 1102 36-37 0.666543342550824 1102 38-39 0.8092529515731783 1102 40-41 0.763844970730446 1102 42-43 0.8594269443999565 1102 44-45 0.6278832858207153 1102 46-47 0.723964077872381 1102 48-49 0.7032543400272502 1102 50-51 0.6331967861564536 1102 52-53 0.8066701984202069 1102 54-55 0.9373436509381747 1102 56-57 1.0563528477231046 1102 58-59 1.0179497875449925 1102 60-61 0.8374880849728754 1102 62-63 0.8989594572647732 1102 64-65 0.9550035493346059 1102 66-67 0.7663297167760561 1102 68-69 1.0313157857134634 1102 70-71 1.0478669439136041 1102 72-73 0.9747340911061109 1102 74-75 0.8856916234673342 1102 76-77 1.0056491813355528 1102 78-79 0.796780616905334 1102 80-81 1.0546403328702958 1102 82-83 0.9793598752670256 1102 84-85 1.0480126278371493 1102 86-87 1.0989800151998637 1102 88-89 1.3252312131588297 1102 90-91 1.5456488655405423 1102 92-93 1.5176339298387482 1102 94-95 1.6796283445487141 1102 96-97 1.6114267957552926 1102 98-99 1.8736854985772986 1102 100-101 1.9925946733117854 1103 1 0.17758524046302426 1103 2 0.21223122611571554 1103 3 0.22808816835068058 1103 4 0.12557949882189945 1103 5 0.18961322338763154 1103 6 0.22237932447404063 1103 7 0.2093468467808961 1103 8 0.15805427250815285 1103 9 0.177342421130966 1103 10-11 0.16659113941206272 1103 12-13 0.19359908579614427 1103 14-15 0.3228385797140021 1103 16-17 0.381134726887737 1103 18-19 0.3893085106622607 1103 20-21 0.3624021276869982 1103 22-23 0.2803698923988094 1103 24-25 0.2587354105456612 1103 26-27 0.1707490247585639 1103 28-29 0.12636216069377326 1103 30-31 0.21608685745405865 1103 32-33 0.2523948809564942 1103 34-35 0.1840238594386534 1103 36-37 0.3809177910830641 1103 38-39 0.31846083761460164 1103 40-41 0.4751428059704068 1103 42-43 0.5372570664604837 1103 44-45 0.4504272179623854 1103 46-47 0.6502979132074245 1103 48-49 0.6979048755190504 1103 50-51 0.48656586089317955 1103 52-53 0.42913316705418936 1103 54-55 0.5410022506190799 1103 56-57 0.8160879753979415 1103 58-59 0.8176992061878536 1103 60-61 0.6667591197536211 1103 62-63 0.8128913332549743 1103 64-65 0.9420162476008116 1103 66-67 1.0739980376712168 1103 68-69 1.1289770729912867 1103 70-71 1.193881343951631 1103 72-73 1.0950419482020202 1103 74-75 1.239998001126441 1103 76-77 1.0001573664804972 1103 78-79 0.9713155661528212 1103 80-81 1.243522463147432 1103 82-83 1.0332115148829324 1103 84-85 1.21673134886543 1103 86-87 1.4687523804949016 1103 88-89 1.4536724768607492 1103 90-91 1.5224127583331146 1103 92-93 1.78428968950438 1103 94-95 1.4717508329670181 1103 96-97 1.5301883757043093 1103 98-99 1.795422312642291 1103 100-101 1.9846612283914418 1104 1 0.2663746139844072 1104 2 0.32612711504775405 1104 3 0.30102705728547363 1104 4 0.36771636182945144 1104 5 0.38923040135581033 1104 6 0.2708236624260252 1104 7 0.4094696803249178 1104 8 0.3240987845202312 1104 9 0.4668935829545191 1104 10-11 0.5503826198316801 1104 12-13 0.48878654465654137 1104 14-15 0.6215729260109555 1104 16-17 0.5348025199875721 1104 18-19 0.5713264785107199 1104 20-21 0.6387011750384985 1104 22-23 0.5762153094131861 1104 24-25 0.60818803012247 1104 26-27 0.47165802052549566 1104 28-29 0.5301627021528503 1104 30-31 0.5766594931148958 1104 32-33 0.6372128201621479 1104 34-35 0.6212224559414778 1104 36-37 0.701276073947227 1104 38-39 0.8240968592399085 1104 40-41 0.8609190396075235 1104 42-43 0.6880831633557705 1104 44-45 0.7771757369368615 1104 46-47 0.6626035759439759 1104 48-49 0.6427024893535176 1104 50-51 0.7296848414298438 1104 52-53 0.7168123566832918 1104 54-55 0.659276632113631 1104 56-57 0.837046655405544 1104 58-59 0.9953923867228696 1104 60-61 0.8599187234915036 1104 62-63 0.8414280993176604 1104 64-65 0.9289770262442971 1104 66-67 0.8499404270866009 1104 68-69 0.890469170057326 1104 70-71 1.0153955290226762 1104 72-73 0.9808689952671017 1104 74-75 0.9557646229010253 1104 76-77 0.9009723092086723 1104 78-79 0.8184074957686462 1104 80-81 1.1390316026791787 1104 82-83 1.217415148662706 1104 84-85 1.441242011422954 1104 86-87 1.402243662342805 1104 88-89 1.3669835294010078 1104 90-91 1.5018271232478888 1104 92-93 1.6959504761348043 1104 94-95 1.658347930680275 1104 96-97 1.4561836441341605 1104 98-99 1.4613194794838442 1104 100-101 1.4186030835084473 1105 1 0.11917372541304516 1105 2 0.01907212632233879 1105 3 0.021418988700265373 1105 4 0.12248294440183827 1105 5 0.09452889882182092 1105 6 -0.008138070957912191 1105 7 0.24506332636988049 1105 8 0.21283033773404725 1105 9 0.1416440908690575 1105 10-11 0.2510783511169805 1105 12-13 0.2602573758503084 1105 14-15 0.09348134511301254 1105 16-17 0.18631007730935778 1105 18-19 0.3237999106734293 1105 20-21 0.4280140392299714 1105 22-23 0.40189429626321527 1105 24-25 0.3625027920165991 1105 26-27 0.35302899907028973 1105 28-29 0.39546013913795974 1105 30-31 0.2598176610504197 1105 32-33 0.47363168779180853 1105 34-35 0.3866764533963263 1105 36-37 0.41173046210556663 1105 38-39 0.27082443844108894 1105 40-41 0.41756965022126025 1105 42-43 0.3495637063219874 1105 44-45 0.35517885140181704 1105 46-47 0.24106130182640584 1105 48-49 0.4002419504059631 1105 50-51 0.12825765739734152 1105 52-53 0.10497711076335747 1105 54-55 0.1714414643343929 1105 56-57 0.15482143158826034 1105 58-59 0.3798906676275138 1105 60-61 0.33055958884391856 1105 62-63 0.4374780870384072 1105 64-65 0.37569651230176504 1105 66-67 0.5060965187121553 1105 68-69 0.3084459957589196 1105 70-71 0.4552372382946288 1105 72-73 0.4444255691323882 1105 74-75 0.523093997105665 1105 76-77 0.46726799360955695 1105 78-79 0.47268235009206094 1105 80-81 0.5762296446965465 1105 82-83 0.5543268608370298 1105 84-85 0.6907031196813094 1105 86-87 0.6182951570627253 1105 88-89 0.41097478880579885 1105 90-91 0.591511434828341 1105 92-93 0.6621426241109596 1105 94-95 0.7670424786921544 1105 96-97 1.1258402157668534 1105 98-99 1.547815929663571 1105 100-101 1.656294528218325 1106 1 -0.050316181842820384 1106 2 -0.03881112517793284 1106 3 0.06193999251914306 1106 4 0.06015342994466977 1106 5 -0.11527742960153375 1106 6 -0.0330016825236541 1106 7 -0.005195811655212879 1106 8 0.046422809092476314 1106 9 0.014939235440792231 1106 10-11 -0.26438127790648025 1106 12-13 -0.3315865957808981 1106 14-15 -0.18638772199010134 1106 16-17 -0.13694415073210564 1106 18-19 -0.15171154595505243 1106 20-21 0.006389652977922822 1106 22-23 -0.08403723674278041 1106 24-25 -0.10546638419725696 1106 26-27 -0.09812348319921682 1106 28-29 0.17881393073643892 1106 30-31 0.15901978870999045 1106 32-33 0.13273561577871362 1106 34-35 -0.09673871299756343 1106 36-37 -0.10075589905100912 1106 38-39 0.05553529496045684 1106 40-41 -0.10524131540886117 1106 42-43 -0.08394693197588765 1106 44-45 -0.15969430735431445 1106 46-47 0.32447646822028986 1106 48-49 0.427328693450157 1106 50-51 0.3677966644895392 1106 52-53 0.3618715461152391 1106 54-55 0.20519760181392854 1106 56-57 0.20086616699470028 1106 58-59 0.08573491203559058 1106 60-61 -0.019099440070426965 1106 62-63 0.022025277436661383 1106 64-65 0.15418123679712892 1106 66-67 0.3990793337694356 1106 68-69 0.34633470279655754 1106 70-71 0.3152481493693706 1106 72-73 0.11696375789398061 1106 74-75 0.3449707019610919 1106 76-77 0.5431817961191037 1106 78-79 0.5680860598574711 1106 80-81 0.28825228517663604 1106 82-83 0.2980052569090397 1106 84-85 0.3097224868389752 1106 86-87 0.25059875771739115 1106 88-89 0.5766935558543516 1106 90-91 0.8669342389745509 1106 92-93 0.841827566827817 1106 94-95 0.7141987798378153 1106 96-97 0.5131765496457419 1106 98-99 0.7272826508856092 1106 100-101 0.7532326078691689 1107 1 0.41823771535994325 1107 2 0.4118661733107203 1107 3 0.4291125046977555 1107 4 0.3952440970586437 1107 5 0.3785415760754489 1107 6 0.42410853796913983 1107 7 0.3967970547637236 1107 8 0.12470771702909644 1107 9 0.472974401702011 1107 10-11 0.4869918464169132 1107 12-13 0.5087937628946264 1107 14-15 0.6774974103595923 1107 16-17 0.6544034996750412 1107 18-19 0.7443334579636485 1107 20-21 0.671941677860481 1107 22-23 0.6391522087667596 1107 24-25 0.5664416418237934 1107 26-27 0.5796701736566447 1107 28-29 0.6983933710368433 1107 30-31 0.6018674275869031 1107 32-33 0.654008894607216 1107 34-35 0.8105476357442711 1107 36-37 0.633486458853092 1107 38-39 0.7856746275526945 1107 40-41 0.9557269208541328 1107 42-43 0.8276634118746031 1107 44-45 0.7733475796108706 1107 46-47 0.8341904521177668 1107 48-49 0.6674492586147664 1107 50-51 0.7170289247531088 1107 52-53 0.5461897350410965 1107 54-55 0.7872600034318253 1107 56-57 0.7283002227523738 1107 58-59 0.6447924466318184 1107 60-61 0.966775077515706 1107 62-63 1.1185592263896424 1107 64-65 1.097077365829584 1107 66-67 1.224882477637351 1107 68-69 1.4185539187344354 1107 70-71 1.0662180314545822 1107 72-73 1.1733450149799403 1107 74-75 1.2245317381151644 1107 76-77 1.1673930413494524 1107 78-79 1.175030229208474 1107 80-81 1.1725353463806876 1107 82-83 1.4026970822269682 1107 84-85 1.3847779820055948 1107 86-87 1.365266445362444 1107 88-89 1.3927493887146305 1107 90-91 1.5018842372529093 1107 92-93 1.5351109063369357 1107 94-95 1.41157472006509 1107 96-97 1.2093645600362173 1107 98-99 1.383378305693828 1107 100-101 1.5083524259378969 1108 1 0.12472460248542205 1108 2 0.09908793768589774 1108 3 0.16775802787349292 1108 4 0.33095520004179946 1108 5 0.2953879256209362 1108 6 0.17764287291174696 1108 7 0.14104431836108233 1108 8 0.2242468869612395 1108 9 0.06347236571180304 1108 10-11 0.22592610945518743 1108 12-13 0.2285424157797351 1108 14-15 0.2166954057690731 1108 16-17 0.0985504214929449 1108 18-19 0.06218958631132665 1108 20-21 0.08827867906315134 1108 22-23 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2316 92-93 -0.2850941614376943 2316 94-95 -0.3962436581522155 2316 96-97 -0.32479703580824903 2316 98-99 -0.04631080803819643 2316 100-101 0.36943729406266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7296.0 3 1423.0 4 1440.0 5 1686.0 6 1967.0 7 2244.0 8 2864.0 9 3677.0 10 4541.0 11 5757.0 12 7444.0 13 9609.0 14 11424.0 15 13464.0 16 16853.0 17 21260.0 18 18440.0 19 14032.0 20 14092.0 21 15003.0 22 16628.0 23 18495.0 24 20967.0 25 23534.0 26 27428.0 27 31958.0 28 37763.0 29 46048.0 30 55587.0 31 68297.0 32 85991.0 33 110669.0 34 145220.0 35 196371.0 36 267624.0 37 327802.0 38 217473.0 39 22098.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87991116768741 18.94395663864799 14.496357430386626 22.679774763277987 2 15.09357164369256 19.83823646946675 41.096574870812006 23.97161701602868 3 19.332574024657067 24.38947454216545 29.618966062924528 26.658985370252953 4 12.399630757634943 16.285991338471813 37.17057451698377 34.14380338690947 5 13.534448672502197 37.84793918795531 33.84186475872987 14.775747380812618 6 32.09657259738797 35.94623171901191 17.33637229663004 14.620823386970084 7 28.994129783665684 33.072446582829606 20.034843666285685 17.898579967219028 8 26.643508210004324 35.24161413878784 19.16432807860618 18.950549572601656 9 26.608778260533793 17.053488591054165 20.420579582276755 35.91715356613528 10-11 25.298003086415672 26.89376197572919 26.684747896437134 21.123487041418006 12-13 26.08116592378906 24.143389923205127 27.686485022645996 22.088959130359815 14-15 22.666400788958597 25.51434245383791 26.24326699525837 25.575989761945124 16-17 22.013682561609077 29.165877789847443 26.672216128626232 22.148223519917245 18-19 21.84044764357755 27.275583221827915 29.54561320929347 21.338355925301062 20-21 22.79098164080451 26.247721428016163 29.52320666403312 21.438090267146205 22-23 22.344307717856545 26.224205982979665 29.55864643322366 21.87283986594013 24-25 22.167689334601224 26.455067309919684 29.671896535128404 21.705346820350687 26-27 21.927522105593845 26.83710852115838 29.500198592032735 21.735170781215043 28-29 21.608726227158755 27.043391261871708 29.718501208493812 21.629381302475725 30-31 21.769058117483215 26.59016541713686 29.643092606780385 21.99768385859954 32-33 21.549666453585566 26.707013665313156 29.624734827757386 22.118585053343885 34-35 22.30501252609217 26.66609719866702 29.376429895800577 21.65246037944023 36-37 22.192319153937664 26.434794197229976 29.359395364542586 22.013491284289774 38-39 21.845213356049573 26.8089181337572 29.488255344080187 21.857613166113037 40-41 22.064602913288464 26.748246710276725 29.398240986490016 21.7889093899448 42-43 22.024077882263285 26.65669966055945 29.1145697127646 22.204652744412666 44-45 21.97676755390319 26.91536789554125 28.98505465680182 22.122809893753733 46-47 22.227951407343475 27.135474426768585 28.497257659435732 22.139316506452207 48-49 22.27729318504108 27.29294702027038 28.299748597131796 22.130011197556744 50-51 22.13985877504368 27.40936222923972 28.23306428430322 22.21771471141338 52-53 22.540818072817213 27.393041929399352 28.010322124669067 22.05581787311436 54-55 22.164938230863388 27.234611130370308 28.183432877379555 22.417017761386745 56-57 22.382868763523074 27.59836020499024 27.949563560298895 22.069207471187788 58-59 22.50941943098203 27.146362530506902 28.05037938144657 22.293838657064498 60-61 22.327586958952693 27.200862445221485 28.541348279141033 21.930202316684788 62-63 22.197987841390823 27.27282581252371 28.43345877930102 22.095727566784447 64-65 22.03938419857542 27.326644253410965 28.30153363455607 22.33243791345755 66-67 22.057286702904307 27.51556261356207 28.053073224585162 22.37407745894846 68-69 22.070898930675963 27.274306322064106 28.32216298130091 22.332631765959025 70-71 22.11003037745776 27.347828500876425 28.252569129205984 22.289571992459834 72-73 22.254443454513247 27.273623738608816 28.159776657422412 22.31215614945552 74-75 22.006545739531603 27.69017573232378 27.92198417663449 22.381294351510125 76-77 22.168474142667307 27.44387341516461 27.97932132532612 22.408331116841968 78-79 22.19829683183647 27.534049614396977 27.91735234767306 22.3503012060935 80-81 22.04525914261754 27.747623464068806 27.90971648658681 22.297400906726846 82-83 22.285560215222883 27.70197631279482 27.814345813106073 22.198117658876228 84-85 22.114029126226427 27.629920928901036 27.768089830139747 22.48796011473279 86-87 22.22681007556036 27.500888199007335 27.880725447113036 22.391576278319274 88-89 22.211318917178442 27.545008957775924 27.666005683171512 22.57766644187412 90-91 22.12416215883245 27.626015568055852 27.825381646846825 22.424440626264875 92-93 22.4316449157395 27.418461120360888 27.83956534451555 22.310328619384066 94-95 22.1981508039839 27.270605185415274 27.842838165608125 22.688405844992698 96-97 22.301261114926906 27.19531868386364 27.86777936225686 22.635640838952593 98-99 22.202612491988656 27.354278111627927 27.580922526318464 22.862186870064956 100-101 22.43875165058271 26.74394720250188 28.137984088316585 22.679317058598826 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2286.0 1 1339.0 2 445.5 3 485.0 4 397.5 5 361.0 6 590.5 7 1214.0 8 2071.5 9 2544.0 10 2301.5 11 1980.5 12 2108.0 13 2326.0 14 2500.5 15 2657.0 16 2717.0 17 2808.0 18 2963.5 19 3161.0 20 3463.5 21 4044.0 22 4861.0 23 5835.5 24 6991.0 25 8678.0 26 11007.0 27 13805.5 28 16183.0 29 18708.5 30 22186.0 31 25576.5 32 29617.5 33 33589.0 34 37662.0 35 41518.5 36 46049.0 37 50922.5 38 53382.5 39 54381.5 40 56058.0 41 59613.5 42 62376.0 43 65547.0 44 69148.0 45 71537.0 46 71951.0 47 72940.0 48 77699.0 49 77277.0 50 71831.0 51 67372.5 52 63654.0 53 62072.0 54 60518.0 55 55814.0 56 49894.0 57 44931.5 58 40489.5 59 35538.0 60 29668.0 61 23934.0 62 19108.5 63 14725.5 64 11067.0 65 8634.0 66 6570.0 67 5089.5 68 3753.5 69 2535.5 70 1892.5 71 1459.5 72 1194.0 73 1025.5 74 853.0 75 671.5 76 469.0 77 298.0 78 196.0 79 142.0 80 96.5 81 62.0 82 48.0 83 36.0 84 22.5 85 17.5 86 14.0 87 7.5 88 7.5 89 6.5 90 5.5 91 6.0 92 6.0 93 3.5 94 2.5 95 2.5 96 1.5 97 1.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31517999992610085 2 0.27300468256871435 3 0.2657731200180736 4 0.27442988102759974 5 0.27669964153619503 6 0.20670656166648982 7 0.197521949375895 8 0.19694131296671952 9 0.20792061961294778 10-11 0.2074719460240394 12-13 0.20332831346765037 14-15 0.2047799044905892 16-17 0.1899736760566131 18-19 0.1944076268175899 20-21 0.18400895658053718 22-23 0.19403813092084182 24-25 0.19356306476788004 26-27 0.1932991391273457 28-29 0.20567725166840595 30-31 0.20208786295713901 32-33 0.20884435935481796 34-35 0.2016127968041772 36-37 0.18730802708721633 38-39 0.17498269967426297 40-41 0.17115577788651515 42-43 0.1883109445212468 44-45 0.18912911400690324 46-47 0.18844290734151398 48-49 0.20491186731085637 50-51 0.20066266449825362 52-53 0.21982366600104622 54-55 0.19010563887688026 56-57 0.1721323027564922 58-59 0.17432288557292713 60-61 0.16397700046398125 62-63 0.16999450506816408 64-65 0.16793588507199628 66-67 0.16688018250985895 68-69 0.1705751414773396 70-71 0.17250179865324022 72-73 0.16933469096682824 74-75 0.18326996478704105 76-77 0.17661903864557585 78-79 0.17994450171630844 80-81 0.1728712945499883 82-83 0.15547859483877574 84-85 0.15558416509498948 86-87 0.15861930996113432 88-89 0.16360750456723322 90-91 0.17041678609301902 92-93 0.16553416174313385 94-95 0.16418814097640874 96-97 0.16944026122304198 98-99 0.17973336120388098 100-101 0.16339636405480576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1894473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.726558791417645 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17107953947621 40.162389035439425 2 13.075344912800754 13.788358845257054 3 4.4335350457743035 7.012951387344881 4 2.033979493239997 4.289789573234263 5 1.1018591694966617 2.9048621140164177 6 0.6791851808961396 2.1486658422467957 7 0.4458821949923292 1.6456883637816573 8 0.316245457936837 1.3339627784340347 9 0.22961089735357423 1.0895933230617054 >10 1.331464577349658 12.986468116169398 >50 0.1178564824486808 4.321854684915956 >100 0.059647215795403 5.65614865288108 >500 0.003407774487043899 1.1723077532514208 >1k 7.016006296855086E-4 0.7269650387110195 >5k 2.0045732276728815E-4 0.7599944912549557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8095 0.4272956120250856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6286 0.3318073152797638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4139 0.2184776452343211 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2486 0.13122382847367053 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2229 0.11765805055020577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.0557025621373332E-4 0.0 0.0 0.0 0.0 16-17 1.0557025621373332E-4 0.0 0.0 7.917769216029999E-5 0.0 18-19 1.0557025621373332E-4 0.0 0.0 1.3196282026716664E-4 0.0 20-21 1.0557025621373332E-4 0.0 0.0 1.5835538432059997E-4 0.0 22-23 1.0557025621373332E-4 0.0 0.0 2.1114051242746663E-4 0.0 24-25 1.0557025621373332E-4 0.0 0.0 2.639256405343333E-4 0.0 26-27 1.0557025621373332E-4 0.0 0.0 5.278512810686666E-4 0.0 28-29 1.0557025621373332E-4 0.0 0.0 0.0013196282026716665 0.0 30-31 1.3196282026716664E-4 0.0 0.0 0.003958884608014999 0.0 32-33 1.5835538432059997E-4 0.0 0.0 0.006994029474159832 0.0 34-35 1.5835538432059997E-4 0.0 0.0 0.010715381005693931 0.0 36-37 1.5835538432059997E-4 0.0 0.0 0.015413257407205064 0.0 38-39 2.639256405343333E-4 0.0 0.0 0.021034873550586364 0.0 40-41 2.903182045877666E-4 0.0 0.0 0.026049460720738694 0.0 42-43 3.694958967480666E-4 0.0 0.0 0.033175453015165696 0.0 44-45 4.2228102485493326E-4 0.0 0.0 0.041251577615516295 0.0 46-47 4.486735889083666E-4 0.0 0.0 0.050726508110698856 0.0 48-49 4.750661529617999E-4 0.0 0.0 0.06387000500930866 0.0 50-51 4.750661529617999E-4 0.0 0.0 0.07843870036680385 0.0 52-53 4.750661529617999E-4 0.0 0.0 0.09976389212197799 0.0 54-55 4.750661529617999E-4 0.0 0.0 0.13362555180253294 0.0 56-57 4.750661529617999E-4 0.0 0.0 0.1820295142765297 0.0 58-59 4.750661529617999E-4 0.0 0.0 0.22826928649814487 0.0 60-61 4.750661529617999E-4 0.0 0.0 0.27712192256104995 0.0 62-63 4.750661529617999E-4 0.0 0.0 0.3313058565627486 0.0 64-65 4.750661529617999E-4 0.0 0.0 0.3916392579888972 0.0 66-67 4.750661529617999E-4 0.0 0.0 0.4741688057839832 0.0 68-69 4.750661529617999E-4 0.0 0.0 0.5792112107166478 0.0 70-71 5.014587170152332E-4 0.0 0.0 0.6810073302707402 0.0 72-73 5.278512810686666E-4 0.0 0.0 0.7896919090427786 0.0 74-75 5.278512810686666E-4 0.0 0.0 0.90793059600216 0.0 76-77 5.278512810686666E-4 0.0 0.0 1.0356178208926705 0.0 78-79 5.278512810686666E-4 0.0 0.0 1.1739940342248212 0.0 80-81 6.334215372823999E-4 0.0 0.0 1.333431513671612 0.0 82-83 6.334215372823999E-4 0.0 0.0 1.5005228366938985 0.0 84-85 6.334215372823999E-4 2.639256405343333E-5 0.0 1.6903117648021375 0.0 86-87 6.334215372823999E-4 5.278512810686666E-5 0.0 1.89596262390649 0.0 88-89 6.862066653892665E-4 5.278512810686666E-5 0.0 2.1247861542497573 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10055 0.0 33.431248 1 GGTATCA 6550 0.0 31.939388 1 GTACATG 9510 0.0 27.0478 1 ATCAACG 12345 0.0 26.990793 3 TCAACGC 12520 0.0 26.579782 4 TATCAAC 12630 0.0 26.457706 2 CAACGCA 12610 0.0 26.390078 5 AACGCAG 12755 0.0 26.149382 6 TACATGG 9795 0.0 26.108269 2 ACATGGG 10065 0.0 24.13214 3 ACGCAGA 14490 0.0 22.854366 7 CGCAGAG 14545 0.0 22.800611 8 GAGTACT 7140 0.0 22.42135 12-13 AGTACTT 7400 0.0 21.440989 12-13 GTACTTT 7465 0.0 21.380718 14-15 GTGTACG 180 5.1283587E-6 21.131615 1 GCAGAGT 15520 0.0 20.969696 9 CATGGGG 3995 0.0 20.467714 4 ACCGTCG 140 4.8449566E-4 20.362354 8 ATACCGT 235 1.1297743E-7 20.217941 6 >>END_MODULE