FastQCFastQC Report
Thu 26 May 2016
SRR937602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894473
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90850.47955288885088365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77340.40824018077850677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.2900542789472323No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25490.13454929154440312No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.10509519006077152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75300.033.509591
GTATCAA110300.031.3635671
ATCAACG138300.024.769963
TCAACGC138900.024.6629624
TATCAAC140450.024.492272
CAACGCA140550.024.373435
AACGCAG142750.024.0900176
GTACATG90150.023.8255161
TACATGG91900.023.1102312
GAGTACT85400.022.22122412-13
ACATGGG93600.021.8275433
GGACCGC2405.9371814E-921.7698846
ACGCAGA161400.021.2786377
GTACTTT89100.021.27011714-15
CGCAGAG161900.021.12498
AGTACTT88500.020.98662412-13
AGAGTAC139200.020.20186610-11
CATGGGG36250.019.5294724
GCAGAGT173150.019.5054599
GTAGCCC9650.018.7097743
Produced by FastQC (version 0.11.5)