##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937602_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1894473 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99297218804385 31.0 31.0 34.0 30.0 34.0 2 32.194105695884815 33.0 31.0 34.0 30.0 34.0 3 32.29313745827996 34.0 31.0 34.0 30.0 34.0 4 35.977192601847584 37.0 35.0 37.0 35.0 37.0 5 35.87912100093271 37.0 35.0 37.0 35.0 37.0 6 35.91530626195253 37.0 35.0 37.0 35.0 37.0 7 35.85444870420428 37.0 35.0 37.0 35.0 37.0 8 35.82883155368274 37.0 35.0 37.0 35.0 37.0 9 37.46451598940708 39.0 37.0 39.0 35.0 39.0 10-11 37.38427441298979 39.0 37.0 39.0 34.0 39.0 12-13 37.275183916582606 39.0 37.0 39.0 34.0 39.0 14-15 38.422217154849925 40.0 38.0 41.0 34.0 41.0 16-17 38.37663772458093 40.0 38.0 41.0 33.5 41.0 18-19 38.35994126070944 40.0 38.0 41.0 33.5 41.0 20-21 38.24566726472217 40.0 38.0 41.0 34.0 41.0 22-23 38.07650676467809 40.0 37.0 41.0 33.0 41.0 24-25 38.150651922724684 40.0 37.5 41.0 33.0 41.0 26-27 38.028470450621356 40.0 37.0 41.0 33.0 41.0 28-29 37.86834966769122 40.0 37.0 41.0 33.0 41.0 30-31 37.909832180242205 40.0 37.0 41.0 33.0 41.0 32-33 37.83380391275041 40.0 37.0 41.0 33.0 41.0 34-35 37.684137224441834 40.0 37.0 41.0 33.0 41.0 36-37 37.49975771626199 40.0 36.5 41.0 33.0 41.0 38-39 37.35209105645739 40.0 36.0 41.0 32.0 41.0 40-41 37.15429805544866 39.0 36.0 41.0 31.5 41.0 42-43 36.91471242926133 39.0 36.0 41.0 31.0 41.0 44-45 36.58270822545373 39.0 35.0 41.0 30.5 41.0 46-47 36.21819788405536 39.0 35.0 40.0 30.0 41.0 48-49 35.88306906458946 39.0 35.0 40.0 29.0 41.0 50-51 35.61327134247888 39.0 35.0 40.0 28.0 41.0 52-53 35.347393180055874 38.0 35.0 40.0 27.5 41.0 54-55 35.100058169211174 38.0 34.0 40.0 27.0 41.0 56-57 34.80748419217376 38.0 34.0 40.0 26.0 41.0 58-59 34.512938426675916 38.0 34.0 40.0 26.0 41.0 60-61 34.125906782519465 37.0 33.0 40.0 25.5 41.0 62-63 33.75426279498309 37.0 33.0 39.5 24.5 41.0 64-65 33.324616397277765 36.0 32.5 39.0 23.0 40.0 66-67 32.860527967408345 36.0 32.0 39.0 22.0 40.0 68-69 32.39743955179092 35.0 31.0 38.0 21.5 40.0 70-71 31.95015790671073 35.0 31.0 38.0 20.0 40.0 72-73 31.41912341849158 35.0 30.5 37.0 18.5 39.0 74-75 30.87950949947558 34.0 30.0 36.5 17.5 39.0 76-77 29.69613686761437 33.0 28.5 35.0 16.0 37.5 78-79 30.321553540219362 34.0 30.0 36.0 16.0 37.5 80-81 30.10775160163275 34.0 30.0 35.0 12.0 37.0 82-83 29.722684092093157 34.0 29.0 35.0 7.5 37.0 84-85 29.28731077191388 34.0 29.0 35.0 6.0 36.0 86-87 28.810553383447534 33.5 29.0 35.0 2.0 36.0 88-89 28.358742774375777 33.0 28.5 35.0 2.0 35.5 90-91 27.734548869263378 33.0 26.5 35.0 2.0 35.0 92-93 27.15527194106224 32.5 26.0 34.5 2.0 35.0 94-95 26.561120691611862 32.0 25.0 34.0 2.0 35.0 96-97 25.865147985745907 32.0 24.5 34.0 2.0 35.0 98-99 24.792299494371257 31.5 19.0 34.0 2.0 35.0 100-101 22.011413464324907 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3535140009458786 1101 2 0.38591882433827607 1101 3 0.24890524997416463 1101 4 0.18500063653126375 1101 5 0.2304912392636851 1101 6 0.25086814163540083 1101 7 0.27088068781935704 1101 8 0.2581516957733001 1101 9 0.2869368361148332 1101 10-11 0.3337567828180923 1101 12-13 0.3547178760924723 1101 14-15 0.35499599933319104 1101 16-17 0.40040844083240046 1101 18-19 0.4825229798981212 1101 20-21 0.5526366783841752 1101 22-23 0.3965313666527024 1101 24-25 0.34147767963398223 1101 26-27 0.35328736472119715 1101 28-29 0.50990322541859 1101 30-31 0.5756469580893935 1101 32-33 0.48838938673203103 1101 34-35 0.48929538394240524 1101 36-37 0.38889321200444016 1101 38-39 0.40249150086223295 1101 40-41 0.3535343177962247 1101 42-43 0.2796549807443043 1101 44-45 0.46702012187726183 1101 46-47 0.3536417186965082 1101 48-49 0.46475220032131404 1101 50-51 0.3482070535128656 1101 52-53 0.4669759866355321 1101 54-55 0.6035784698042335 1101 56-57 0.47159201450963906 1101 58-59 0.3377383794386475 1101 60-61 0.40101006801101846 1101 62-63 0.4146996117338446 1101 64-65 0.4963664172331619 1101 66-67 0.550685625034582 1101 68-69 0.39804803175396586 1101 70-71 0.40280539088934475 1101 72-73 0.34861301310683857 1101 74-75 0.3493827199110662 1101 76-77 0.2524761595386664 1101 78-79 0.29412015640393463 1101 80-81 0.307729465271116 1101 82-83 0.28037031419560776 1101 84-85 0.2916082884846105 1101 86-87 0.23143927893608662 1101 88-89 0.3854879528278552 1101 90-91 0.2817500852667294 1101 92-93 0.4150623030158833 1101 94-95 0.47872740323739293 1101 96-97 0.7131731632687703 1101 98-99 0.6900200833696246 1101 100-101 1.1387252296608814 1102 1 0.40649897148374237 1102 2 0.38673797918540487 1102 3 0.2760491148301938 1102 4 0.14820554985976742 1102 5 0.1935238042415861 1102 6 0.26196989061388365 1102 7 0.2880955156081981 1102 8 0.2916950292362017 1102 9 0.32861827005311284 1102 10-11 0.3450030128765533 1102 12-13 0.4099882486392943 1102 14-15 0.4664945210816711 1102 16-17 0.4911458459693492 1102 18-19 0.4974784151305798 1102 20-21 0.438483191745604 1102 22-23 0.5081207785761137 1102 24-25 0.570365292503574 1102 26-27 0.4499987981335707 1102 28-29 0.46764286679682243 1102 30-31 0.4107896156495201 1102 32-33 0.4128973793510653 1102 34-35 0.4862212667203707 1102 36-37 0.5253179355039492 1102 38-39 0.4759523414877336 1102 40-41 0.502406850899682 1102 42-43 0.5939509191539187 1102 44-45 0.5527328437579015 1102 46-47 0.7601112984980816 1102 48-49 0.721776896153628 1102 50-51 0.6830900452744402 1102 52-53 0.5633415800740522 1102 54-55 0.6890090891607557 1102 56-57 0.5440697835731356 1102 58-59 0.5052957031288514 1102 60-61 0.4556823660576299 1102 62-63 0.5274034286638809 1102 64-65 0.7340027810773933 1102 66-67 0.8123145656095545 1102 68-69 0.7784777193432859 1102 70-71 0.9391984743185269 1102 72-73 0.9612402307699419 1102 74-75 0.7271490109745606 1102 76-77 0.7885299768116916 1102 78-79 0.9378648258952467 1102 80-81 0.8961577454918128 1102 82-83 0.944100818538903 1102 84-85 0.9516610276515287 1102 86-87 1.0702116842216647 1102 88-89 1.1685082390575943 1102 90-91 1.143580013171956 1102 92-93 0.9676607044920758 1102 94-95 1.2279005312999374 1102 96-97 1.45325284365547 1102 98-99 1.3183402900275851 1102 100-101 2.001360878959712 1103 1 0.3228886982074357 1103 2 0.3063726785507015 1103 3 0.2829012284811441 1103 4 0.18210786508048216 1103 5 0.12215677946171866 1103 6 0.1963701523819097 1103 7 0.22451692856822802 1103 8 0.20971213644782694 1103 9 0.2928192553188751 1103 10-11 0.24183173240862033 1103 12-13 0.21086332572242839 1103 14-15 0.34910428676293037 1103 16-17 0.30952931654911 1103 18-19 0.24710708767864276 1103 20-21 0.28524681885481584 1103 22-23 0.3657858133876317 1103 24-25 0.3474540181603416 1103 26-27 0.3188081311704565 1103 28-29 0.3701604263693241 1103 30-31 0.2959608485741114 1103 32-33 0.4803886432627351 1103 34-35 0.36900162908450085 1103 36-37 0.2718060909203217 1103 38-39 0.3351313519430761 1103 40-41 0.2678603234510959 1103 42-43 0.3761933426336199 1103 44-45 0.31507963884751433 1103 46-47 0.24296856920285848 1103 48-49 0.17163084022308794 1103 50-51 0.2460100172518338 1103 52-53 0.10949595329205408 1103 54-55 0.2482780124579662 1103 56-57 0.17898840755707113 1103 58-59 0.1943387815879447 1103 60-61 0.12347790733153374 1103 62-63 0.35315499794361216 1103 64-65 0.3388570957052224 1103 66-67 0.37477924021036557 1103 68-69 0.40597161212187416 1103 70-71 0.30685152541223104 1103 72-73 0.2699517084308667 1103 74-75 0.2335038772449387 1103 76-77 0.13836728071289883 1103 78-79 0.31362988909292966 1103 80-81 0.24767193088565875 1103 82-83 0.4026480619342223 1103 84-85 0.4630469578998593 1103 86-87 0.5067838375293512 1103 88-89 0.6018575133924422 1103 90-91 0.5777664697530049 1103 92-93 0.6877582522346763 1103 94-95 0.9430163762359385 1103 96-97 1.095418750327898 1103 98-99 1.150675772599456 1103 100-101 1.4185900252631463 1104 1 0.20782093319957085 1104 2 0.276207679500871 1104 3 0.17242044256678923 1104 4 0.07499563763462191 1104 5 0.11533703906463444 1104 6 0.14384559255839235 1104 7 0.21188132281433525 1104 8 0.13571869675055837 1104 9 0.2651398453605438 1104 10-11 0.23730369138331042 1104 12-13 0.25257825826037816 1104 14-15 0.34239911756385055 1104 16-17 0.3996695836432238 1104 18-19 0.3178808288413748 1104 20-21 0.2757912523746029 1104 22-23 0.35742569628264675 1104 24-25 0.28712002659343483 1104 26-27 0.18667380194901284 1104 28-29 0.28583134263595156 1104 30-31 0.41048577744908243 1104 32-33 0.39155603013056606 1104 34-35 0.3973047084608652 1104 36-37 0.44428132453816715 1104 38-39 0.31431698757912585 1104 40-41 0.415594099646178 1104 42-43 0.486217496225116 1104 44-45 0.47384378467241106 1104 46-47 0.4641277488069875 1104 48-49 0.34395087944169234 1104 50-51 0.41791267029114465 1104 52-53 0.5114059322781586 1104 54-55 0.5193439652579386 1104 56-57 0.6157809097534752 1104 58-59 0.7097100501059259 1104 60-61 0.5606491003867546 1104 62-63 0.5259633418114404 1104 64-65 0.629683275223968 1104 66-67 0.5595949761237549 1104 68-69 0.5443186747201594 1104 70-71 0.7125362948790048 1104 72-73 0.6276651592547573 1104 74-75 0.34860896051561596 1104 76-77 0.4393462310093774 1104 78-79 0.5323341879915588 1104 80-81 0.6818982861169651 1104 82-83 0.6764487135579103 1104 84-85 0.47078326788104974 1104 86-87 0.5336341210655249 1104 88-89 0.4444766219954701 1104 90-91 0.7258133888618836 1104 92-93 0.8426005378199157 1104 94-95 0.847777140007203 1104 96-97 1.3233895456888654 1104 98-99 1.223355912398354 1104 100-101 1.3341412752183928 1105 1 0.12660093364608827 1105 2 0.1150759789360265 1105 3 0.11769348849119865 1105 4 0.02141511922690853 1105 5 0.023199667717712202 1105 6 -0.007462147835880728 1105 7 0.08200980844760863 1105 8 0.04257777558336073 1105 9 0.17362983218825434 1105 10-11 0.12973660201267734 1105 12-13 0.11846091325802632 1105 14-15 0.07197732523950862 1105 16-17 0.2139528659990475 1105 18-19 0.22137902044236313 1105 20-21 0.30229880969638856 1105 22-23 0.24017920703256834 1105 24-25 0.2317171554809363 1105 26-27 0.009074507447884628 1105 28-29 -0.05788168006949945 1105 30-31 0.0030816672483808816 1105 32-33 0.08324716227765094 1105 34-35 0.15642623957202773 1105 36-37 0.042846047361940975 1105 38-39 0.03954861893364381 1105 40-41 0.03194838957049484 1105 42-43 0.3444641305936287 1105 44-45 0.2474671130196242 1105 46-47 0.1627904265770681 1105 48-49 0.12160431428350194 1105 50-51 0.18954117825033023 1105 52-53 0.1291712199475512 1105 54-55 0.16301596409699215 1105 56-57 0.09938057263217814 1105 58-59 0.20101913005502325 1105 60-61 0.13373995569251207 1105 62-63 0.13623075044263544 1105 64-65 0.21100206694551105 1105 66-67 0.20112508261836126 1105 68-69 0.19600483935144553 1105 70-71 0.40585191632080964 1105 72-73 0.3244485532401562 1105 74-75 0.19240961243229648 1105 76-77 0.009033779847317902 1105 78-79 0.2329065686037346 1105 80-81 0.1823071294113774 1105 82-83 0.3563177162593476 1105 84-85 0.20526229268513774 1105 86-87 0.4759774489662192 1105 88-89 0.37457000012947006 1105 90-91 0.3355134402114821 1105 92-93 0.43896308833229014 1105 94-95 0.5000108476716925 1105 96-97 0.6606802402480412 1105 98-99 0.837266910143434 1105 100-101 0.9106022271953549 1106 1 0.019549651594807926 1106 2 0.04210816660651062 1106 3 0.05240434501056512 1106 4 0.019110154687901115 1106 5 -0.07375887019827587 1106 6 -0.024551618648757767 1106 7 0.052127102501074773 1106 8 -0.047643173680143036 1106 9 0.043228850191525225 1106 10-11 -0.021184019918578656 1106 12-13 -0.24992424768031896 1106 14-15 0.039864668392802116 1106 16-17 0.09532902530074239 1106 18-19 0.01201595443036041 1106 20-21 0.1467341615785429 1106 22-23 0.09779650108603732 1106 24-25 0.10291191816505574 1106 26-27 0.03460642234150413 1106 28-29 0.19402775801015082 1106 30-31 0.057834804182363087 1106 32-33 0.004762437782282802 1106 34-35 -0.008024115037905233 1106 36-37 -0.046317891536034494 1106 38-39 -0.010805990995443437 1106 40-41 -0.015992145072168285 1106 42-43 -0.34084683503647994 1106 44-45 -0.17183866984998275 1106 46-47 0.16625469280729277 1106 48-49 0.24674070271776571 1106 50-51 0.3316374684849137 1106 52-53 0.24947945780898806 1106 54-55 0.16239539672110226 1106 56-57 0.1132990123484916 1106 58-59 0.15209659868568792 1106 60-61 0.24874950288291586 1106 62-63 0.21549288901328367 1106 64-65 0.12655034845124646 1106 66-67 0.07165154197461021 1106 68-69 0.26595846728380224 1106 70-71 0.23432982139446423 1106 72-73 0.3475663928473587 1106 74-75 0.20944452787146872 1106 76-77 0.14303541650852836 1106 78-79 0.19812069844552127 1106 80-81 0.11796588554885545 1106 82-83 0.12239109323697761 1106 84-85 0.09034139797701002 1106 86-87 0.19569921656032818 1106 88-89 -0.04997309861575516 1106 90-91 0.12371584064792529 1106 92-93 0.03182997322045367 1106 94-95 0.3302890800775842 1106 96-97 0.575021484110561 1106 98-99 0.6389240296197016 1106 100-101 0.44236027411297485 1107 1 0.2399764218250766 1107 2 0.30406746583373234 1107 3 0.25081974358858616 1107 4 0.12000663815654633 1107 5 0.07682944016778492 1107 6 0.20673631463131414 1107 7 0.21632740506700543 1107 8 0.16663529754371353 1107 9 0.28571911504254643 1107 10-11 0.24977581033112273 1107 12-13 0.30580152358955814 1107 14-15 0.35307032256607584 1107 16-17 0.37484018135343433 1107 18-19 0.37057806810835103 1107 20-21 0.43896490143091427 1107 22-23 0.4111362534419172 1107 24-25 0.3453652230929549 1107 26-27 0.32225318403624215 1107 28-29 0.4773750065965956 1107 30-31 0.5173600555493323 1107 32-33 0.2543104684659667 1107 34-35 0.2173924027624068 1107 36-37 0.1511763520349092 1107 38-39 0.29497371964942687 1107 40-41 0.1544597547468527 1107 42-43 0.22785834513366154 1107 44-45 0.30547093837166983 1107 46-47 0.3907783283622308 1107 48-49 0.3513210164727667 1107 50-51 0.4492125121339541 1107 52-53 0.2321394097737226 1107 54-55 0.23466847305977723 1107 56-57 0.28424687011028027 1107 58-59 0.2748370285138009 1107 60-61 0.3587434976851469 1107 62-63 0.41491457678027643 1107 64-65 0.26530974598554735 1107 66-67 0.2959922732946083 1107 68-69 0.27265879591121944 1107 70-71 0.2947725633658642 1107 72-73 0.45829737483460775 1107 74-75 0.4932193427480591 1107 76-77 0.4333721972234059 1107 78-79 0.6179900056301832 1107 80-81 0.6155890781849216 1107 82-83 0.5486819501417415 1107 84-85 0.6140704583641572 1107 86-87 0.7369853460697939 1107 88-89 0.7745849380983998 1107 90-91 0.6923685819527883 1107 92-93 0.8547920994079483 1107 94-95 1.050790086050874 1107 96-97 0.9053806492711907 1107 98-99 1.3359417428997844 1107 100-101 1.6173500311599227 1108 1 0.03980829165890043 1108 2 0.0757509408398036 1108 3 -0.020994225621016938 1108 4 -0.0685741680014047 1108 5 -0.13259582718942653 1108 6 -0.15076666553200369 1108 7 -0.005958946923101394 1108 8 0.0279737471229069 1108 9 0.009207978134014638 1108 10-11 0.015532010988493994 1108 12-13 0.022971162000565926 1108 14-15 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2316 48-49 -0.8544764824859783 2316 50-51 -0.8374641776604008 2316 52-53 -0.7501372624124372 2316 54-55 -0.7462147812059783 2316 56-57 -0.8511212258679421 2316 58-59 -0.6741584576925561 2316 60-61 -0.7151499794936171 2316 62-63 -0.6345881609557793 2316 64-65 -0.7044989680403972 2316 66-67 -0.8347959982915185 2316 68-69 -0.5767758793699187 2316 70-71 -0.5270900452885634 2316 72-73 -0.6352817282986329 2316 74-75 -0.6829591366486589 2316 76-77 -0.40627976998471027 2316 78-79 -0.27085147657368225 2316 80-81 -0.6408184432242763 2316 82-83 -0.535276982124266 2316 84-85 -0.4316312790908512 2316 86-87 -0.5209882890409503 2316 88-89 -0.3841969809919341 2316 90-91 -0.4276871547640262 2316 92-93 -0.29448816552769586 2316 94-95 -0.3607456535103424 2316 96-97 -0.1386567749204417 2316 98-99 -0.19471514444500215 2316 100-101 0.2721379370373356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 34.0 5 44.0 6 98.0 7 251.0 8 571.0 9 1045.0 10 1779.0 11 2833.0 12 4283.0 13 6279.0 14 8225.0 15 11277.0 16 15081.0 17 20090.0 18 17994.0 19 12078.0 20 10695.0 21 11246.0 22 11926.0 23 13573.0 24 15666.0 25 18895.0 26 23261.0 27 28682.0 28 35681.0 29 43720.0 30 54775.0 31 68367.0 32 85476.0 33 110646.0 34 149086.0 35 205788.0 36 292097.0 37 375513.0 38 224289.0 39 13115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.526087381178016 19.889552640679156 15.31316736312209 22.271192615020734 2 15.70626765332628 20.701482681463393 39.77433302031753 23.8179166448928 3 19.624507712698993 24.43497479246207 30.2182189981066 25.72229849673234 4 13.067869605816082 17.01862947492994 36.790164642266895 33.12333627698709 5 13.510318534291365 38.03525730206508 33.81692580697448 14.637498356669074 6 32.167784919605616 36.50872828485811 16.779494877995095 14.543991917541184 7 28.82601124428799 33.269146617555386 19.874709219925542 18.030132918231086 8 26.87475619868956 35.278148593302724 18.985754877477802 18.861340330529917 9 26.482404341471216 16.842520320954694 20.294245418118916 36.380829919455174 10-11 25.340952338724275 27.04443399298908 26.517136955765537 21.097476712521107 12-13 26.068316964957333 24.223146581644368 27.699330396615295 22.009206056783 14-15 22.66260854601781 25.604666838746184 26.284592073890735 25.44813254134527 16-17 21.979199492418207 29.15399163778001 26.83619666260749 22.0306122071943 18-19 21.73913272978818 27.15781644816263 29.81897868167031 21.284072140378882 20-21 22.547932855205644 26.09451282757791 30.051022104828096 21.30653221238835 22-23 22.24570304839892 26.103174345530256 29.996513506401712 21.654609099669113 24-25 22.092059926971753 26.30262513707641 30.183652705436476 21.421662230515352 26-27 21.924812038012835 26.658469103818465 29.96810973860437 21.44860911956433 28-29 21.517641311413623 26.893528206857948 30.148995003588098 21.439835478140335 30-31 21.723006873151533 26.400930496238267 30.117109085217898 21.758953545392306 32-33 21.628099212815385 26.511832050390794 30.039990013053764 21.820078723740057 34-35 22.27025668879947 26.441099978727596 29.807867412203816 21.480775920269117 36-37 22.16460725489358 26.22460177579728 29.857406254932112 21.753384714377034 38-39 21.817128514665693 26.617884397899676 29.94131611833901 21.623670969095617 40-41 21.947485910255306 26.558317581288204 29.866299843280125 21.62789666517636 42-43 22.00813018860454 26.43966370583499 29.56627555532092 21.985930550239548 44-45 22.043914059477228 26.68491448545321 29.324936275154094 21.94623517991547 46-47 22.27645894135203 27.038997124794072 28.792783006144717 21.891760927709182 48-49 22.341199900975102 27.13923608306901 28.57610005526603 21.94346396068986 50-51 22.21258893634272 27.274471581278803 28.476600088784586 22.03633939359389 52-53 22.613043310725462 27.35462579830908 28.17197711447986 21.860353776485596 54-55 22.200448356878137 27.180302912736153 28.34186604929181 22.2773826810939 56-57 22.39348357036495 27.477641539362136 28.176120747036247 21.952754143236668 58-59 22.622729381733073 27.075577218572132 28.190029628292407 22.11166377140239 60-61 22.362525092730273 27.09896103032347 28.784786059236527 21.753727817709727 62-63 22.226867313495628 27.192232351688304 28.645433321034396 21.935467013781672 64-65 22.13079838034113 27.162012865847124 28.504681776937442 22.202506976874307 66-67 22.104643349364178 27.385293957739172 28.241468735632548 22.268593957264105 68-69 22.15350767918449 27.095491514258324 28.5447343639811 22.206266442576084 70-71 22.187093719467104 27.228126238800975 28.44772662371013 22.137053418021793 72-73 22.32449340792928 27.118992986440027 28.363112063354823 22.193401542275872 74-75 22.154874245997235 27.5504395022889 28.013893049384457 22.280793202329406 76-77 22.182422235629645 27.254043736701444 28.30167017423843 22.26186385343048 78-79 22.24557964140951 27.38909448696287 28.150256034263883 22.21506983736374 80-81 21.984380855859424 27.67584073645927 28.12029221893194 22.21948618874936 82-83 22.29821998947439 27.59615440447164 27.981020118484373 22.124605487569593 84-85 22.13630797166475 27.53787358930777 27.91914318592105 22.406675253106425 86-87 22.175956057436554 27.356789988561463 28.08736255412455 22.379891399877433 88-89 22.100684464756164 27.48574405652654 27.85927801557478 22.554293463142518 90-91 22.052805693451198 27.4855991551258 27.988226453104176 22.47336869831883 92-93 22.33081559753952 27.28463540690668 28.0748215687382 22.309727426815602 94-95 22.055210076892095 27.17053766403638 28.13484277685668 22.63940948221484 96-97 22.185853268956592 27.06351581679971 28.164244093212204 22.586386821031496 98-99 22.061195791482884 27.229229261989268 27.725364275745285 22.984210670782563 100-101 21.9748173787435 26.886716745403056 28.374903119397025 22.76356275645642 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 376.0 1 269.5 2 262.5 3 375.5 4 308.5 5 275.0 6 574.5 7 1420.5 8 2340.5 9 2711.0 10 2480.5 11 2179.0 12 2260.0 13 2462.5 14 2680.0 15 2842.5 16 2979.0 17 3129.0 18 3295.0 19 3459.5 20 3798.5 21 4444.0 22 5192.0 23 6082.5 24 7255.5 25 8903.0 26 11164.5 27 13988.5 28 16223.0 29 18634.5 30 22199.5 31 25613.0 32 29374.5 33 33457.5 34 37496.0 35 41430.5 36 46305.0 37 50939.5 38 53527.0 39 54516.0 40 55699.0 41 59346.5 42 62690.0 43 66229.0 44 69692.0 45 71978.0 46 72039.0 47 73158.0 48 78586.0 49 77986.5 50 72309.5 51 67684.5 52 63514.5 53 61875.5 54 60221.0 55 55187.5 56 49412.5 57 44254.0 58 39965.5 59 35554.5 60 29668.5 61 23708.0 62 18910.5 63 14420.5 64 10603.5 65 8263.5 66 6304.0 67 4935.5 68 3534.5 69 2264.0 70 1630.0 71 1241.5 72 1023.0 73 886.5 74 740.0 75 635.5 76 460.5 77 270.0 78 173.5 79 117.0 80 83.0 81 66.0 82 48.0 83 26.5 84 15.5 85 10.5 86 6.0 87 2.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018897075862258264 2 0.0 3 0.0 4 1.0557025621373332E-4 5 0.023964448160517463 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003589388711266933 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010293099980838998 24-25 2.639256405343333E-5 26-27 3.958884608015E-4 28-29 7.125992294426999E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.639256405343333E-5 40-41 5.278512810686666E-4 42-43 0.01572996817584626 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.278512810686666E-5 70-71 0.0 72-73 0.0 74-75 2.639256405343333E-5 76-77 0.0 78-79 0.0 80-81 1.5835538432059997E-4 82-83 0.002428115892915866 84-85 6.862066653892665E-4 86-87 0.0 88-89 0.0 90-91 0.011955831516205299 92-93 0.002428115892915866 94-95 0.0 96-97 0.0 98-99 0.0015307687150991332 100-101 0.020533414833571132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1894473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.768616173439796 #Duplication Level Percentage of deduplicated Percentage of total 1 74.45115980673832 37.797823558957255 2 14.036148636924489 14.25191685402739 3 4.742861921792944 7.223656094133868 4 2.152372038585773 4.370917995576211 5 1.2194056359811762 3.095376834642879 6 0.7319752186141705 2.22968213533755 7 0.46172569555143533 1.640882223040574 8 0.3343068620363181 1.3577837410295142 9 0.2590289010951271 1.1835484971773769 >10 1.4142969094981603 13.425036029650474 >50 0.12610369043580116 4.441315690212179 >100 0.06644309835742565 6.167165433126439 >500 0.0034415571208632287 1.255517785649683 >1k 4.1715843889251255E-4 0.37793134373413056 >5k 3.128688291693844E-4 1.1814457837045937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9085 0.47955288885088365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7734 0.40824018077850677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5495 0.2900542789472323 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2549 0.13454929154440312 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1991 0.10509519006077152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.639256405343333E-5 0.0 12-13 5.278512810686666E-5 0.0 0.0 5.278512810686666E-5 0.0 14-15 1.0557025621373332E-4 0.0 0.0 5.278512810686666E-5 0.0 16-17 1.0557025621373332E-4 0.0 0.0 1.3196282026716664E-4 0.0 18-19 1.0557025621373332E-4 0.0 0.0 1.847479483740333E-4 0.0 20-21 1.0557025621373332E-4 0.0 0.0 2.1114051242746663E-4 0.0 22-23 1.5835538432059997E-4 0.0 0.0 2.639256405343333E-4 0.0 24-25 2.1114051242746663E-4 0.0 0.0 3.1671076864119995E-4 0.0 26-27 2.1114051242746663E-4 0.0 0.0 5.806364091755332E-4 0.0 28-29 2.1114051242746663E-4 0.0 0.0 0.0013724133307785333 0.0 30-31 2.1114051242746663E-4 0.0 0.0 0.0040380623001753 0.0 32-33 2.3753307648089996E-4 0.0 0.0 0.006994029474159832 0.0 34-35 2.639256405343333E-4 0.0 0.0 0.010662595877587066 0.0 36-37 2.639256405343333E-4 0.0 0.0 0.015096546638563864 0.0 38-39 2.639256405343333E-4 0.0 0.0 0.020665377653838298 0.0 40-41 2.639256405343333E-4 0.0 0.0 0.0258119276442578 0.0 42-43 2.903182045877666E-4 0.0 0.0 0.032990705066791665 0.0 44-45 3.1671076864119995E-4 0.0 0.0 0.0409348668468751 0.0 46-47 3.1671076864119995E-4 0.0 0.0 0.050462582470164524 0.0 48-49 3.1671076864119995E-4 0.0 0.0 0.06344772398445372 0.0 50-51 3.1671076864119995E-4 0.0 0.0 0.07788445652168176 0.0 52-53 3.1671076864119995E-4 0.0 0.0 0.09942078878928334 0.0 54-55 3.1671076864119995E-4 0.0 0.0 0.13325605590578488 0.0 56-57 3.1671076864119995E-4 0.0 0.0 0.18176558863599535 0.0 58-59 3.1671076864119995E-4 0.0 0.0 0.2279789682935571 0.0 60-61 3.1671076864119995E-4 0.0 0.0 0.2767524266643019 0.0 62-63 3.1671076864119995E-4 0.0 0.0 0.3313058565627486 0.0 64-65 3.1671076864119995E-4 0.0 0.0 0.3914809026045766 0.0 66-67 3.1671076864119995E-4 0.0 0.0 0.47422159091209004 0.0 68-69 3.1671076864119995E-4 0.0 0.0 0.5792376032807013 0.0 70-71 3.1671076864119995E-4 0.0 0.0 0.6812184707831677 0.0 72-73 3.1671076864119995E-4 0.0 0.0 0.7904836859643817 0.0 74-75 3.1671076864119995E-4 0.0 0.0 0.9092238316407781 0.0 76-77 3.1671076864119995E-4 0.0 0.0 1.03730694499209 0.0 78-79 3.431033326946333E-4 0.0 0.0 1.1763957575536836 0.0 80-81 4.750661529617999E-4 0.0 0.0 1.336598621358024 0.0 82-83 4.750661529617999E-4 0.0 0.0 1.503901084892738 0.0 84-85 5.014587170152332E-4 0.0 0.0 1.6953263519722899 0.0 86-87 5.278512810686666E-4 0.0 0.0 1.9015842400498713 0.0 88-89 5.278512810686666E-4 0.0 0.0 2.131542650647436 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7530 0.0 33.50959 1 GTATCAA 11030 0.0 31.363567 1 ATCAACG 13830 0.0 24.76996 3 TCAACGC 13890 0.0 24.662962 4 TATCAAC 14045 0.0 24.49227 2 CAACGCA 14055 0.0 24.37343 5 AACGCAG 14275 0.0 24.090017 6 GTACATG 9015 0.0 23.825516 1 TACATGG 9190 0.0 23.110231 2 GAGTACT 8540 0.0 22.221224 12-13 ACATGGG 9360 0.0 21.827543 3 GGACCGC 240 5.9371814E-9 21.769884 6 ACGCAGA 16140 0.0 21.278637 7 GTACTTT 8910 0.0 21.270117 14-15 CGCAGAG 16190 0.0 21.1249 8 AGTACTT 8850 0.0 20.986624 12-13 AGAGTAC 13920 0.0 20.201866 10-11 CATGGGG 3625 0.0 19.529472 4 GCAGAGT 17315 0.0 19.505459 9 GTAGCCC 965 0.0 18.709774 3 >>END_MODULE