FastQCFastQC Report
Thu 26 May 2016
SRR937601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856954
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83570.45003807310251087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62210.3350109911177121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42520.2289771313667436No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26380.14206060031643217No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21920.11804277327278974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105900.034.6605571
GGTATCA68400.030.515651
ATCAACG128000.028.25313
TCAACGC128850.028.0298374
CAACGCA129350.027.9214865
TATCAAC130300.027.9055442
AACGCAG131250.027.5785376
GTACATG94650.025.2172261
ACGCAGA146350.024.5383117
TACCGTC1951.5703336E-824.3602077
CGCAGAG147800.024.3297168
ATACCGT2151.6625563E-924.3035556
TACATGG96450.024.196512
GAGTACT70300.023.41113712-13
ACCGTCG1652.4301498E-623.031478
ACATGGG97150.022.941483
GCAGAGT155250.022.9168039
CATGGGG38550.022.6821824
AGTACTT72550.022.55414612-13
GTACTTT75450.021.65548314-15
Produced by FastQC (version 0.11.5)