##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937601_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856954 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.681724479981735 33.0 31.0 34.0 30.0 34.0 2 31.808112101861436 33.0 31.0 34.0 30.0 34.0 3 31.83144816726747 33.0 31.0 34.0 30.0 34.0 4 35.45400801527663 37.0 35.0 37.0 33.0 37.0 5 35.44985767014153 37.0 35.0 37.0 33.0 37.0 6 35.5086049519805 37.0 35.0 37.0 33.0 37.0 7 35.44375843451157 37.0 35.0 37.0 33.0 37.0 8 35.422908160352925 37.0 35.0 37.0 33.0 37.0 9 37.034862468321776 39.0 37.0 39.0 33.0 39.0 10-11 36.918869288092225 39.0 37.0 39.0 33.0 39.0 12-13 36.78497286416357 39.0 37.0 39.0 33.0 39.0 14-15 37.9127867464676 40.0 38.0 41.0 33.0 41.0 16-17 37.84200927971291 40.0 38.0 41.0 33.0 41.0 18-19 37.781028501513774 40.0 37.5 41.0 33.0 41.0 20-21 37.689460266651736 40.0 37.0 41.0 32.0 41.0 22-23 37.47928327788411 40.0 37.0 41.0 32.0 41.0 24-25 37.29805934880454 39.5 37.0 41.0 32.0 41.0 26-27 37.25720534811309 39.0 37.0 41.0 31.5 41.0 28-29 37.30256807653824 39.5 37.0 41.0 32.0 41.0 30-31 37.12347613349604 39.0 36.5 41.0 31.0 41.0 32-33 36.88718702778852 39.0 36.0 41.0 31.0 41.0 34-35 36.8910102242705 39.0 36.0 41.0 31.0 41.0 36-37 36.785770945322284 39.0 36.0 41.0 30.5 41.0 38-39 36.64748157466475 39.0 36.0 41.0 30.0 41.0 40-41 36.40414329057155 39.0 35.0 41.0 30.0 41.0 42-43 36.13803088283285 39.0 35.0 40.5 30.0 41.0 44-45 35.88763372706055 39.0 35.0 40.0 28.5 41.0 46-47 35.529129962293084 39.0 35.0 40.0 27.0 41.0 48-49 35.163482240270895 38.0 35.0 40.0 26.0 41.0 50-51 34.22821809263988 37.5 33.5 39.5 24.5 40.5 52-53 34.34142552804216 38.0 33.5 39.5 24.0 40.5 54-55 34.68340761268185 38.0 34.0 40.0 24.5 41.0 56-57 34.47578507599003 38.0 34.0 40.0 24.0 41.0 58-59 34.215786713079595 38.0 34.0 40.0 23.0 41.0 60-61 33.87129487321711 37.5 33.0 40.0 22.5 41.0 62-63 33.495358258739856 37.0 33.0 40.0 22.0 41.0 64-65 33.112484746525766 36.5 32.5 39.0 20.5 41.0 66-67 32.685665073017425 36.0 32.0 39.0 18.5 40.5 68-69 32.1922810150386 35.5 31.0 39.0 17.5 40.0 70-71 31.72448025099168 35.0 31.0 38.0 13.0 40.0 72-73 31.248941007693244 35.0 31.0 37.5 9.5 39.5 74-75 30.780146681070185 35.0 30.0 37.0 7.0 39.0 76-77 30.26569155724913 34.0 29.5 36.0 4.5 39.0 78-79 29.713266456788915 34.0 29.0 36.0 2.0 38.5 80-81 29.169895161646437 34.0 29.0 35.0 2.0 37.0 82-83 28.63137643689612 34.0 28.0 35.0 2.0 37.0 84-85 28.14121459120689 33.5 27.0 35.0 2.0 36.0 86-87 27.644395606999417 33.0 26.0 35.0 2.0 36.0 88-89 27.123263419556974 33.0 25.5 35.0 2.0 35.5 90-91 26.589303773814535 32.5 25.0 35.0 2.0 35.0 92-93 25.9659980807279 32.0 23.5 34.5 2.0 35.0 94-95 25.274399635101354 32.0 20.5 34.0 2.0 35.0 96-97 24.47005849364066 31.0 12.5 34.0 2.0 35.0 98-99 23.44480477168524 31.0 2.0 34.0 2.0 35.0 100-101 21.035741326925706 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1690658534014986 1101 2 0.2314443757900655 1101 3 0.17325818853861108 1101 4 0.17662274182752213 1101 5 0.03856510614170361 1101 6 0.2224680814906037 1101 7 0.2581984775980999 1101 8 0.04987249857816067 1101 9 0.20124857281864905 1101 10-11 0.16043341134768951 1101 12-13 0.34041621989744186 1101 14-15 0.3430415902469619 1101 16-17 0.4564452964083827 1101 18-19 0.41679322103575345 1101 20-21 0.4465194929330707 1101 22-23 0.3786528133594871 1101 24-25 0.2509355151424799 1101 26-27 0.39408988554736624 1101 28-29 0.387834499822894 1101 30-31 0.4420697216770577 1101 32-33 0.415683134938881 1101 34-35 0.5974903449232798 1101 36-37 0.41918424818070577 1101 38-39 0.4898191319686731 1101 40-41 0.6166498400280958 1101 42-43 0.5679446019647756 1101 44-45 0.6477579017974762 1101 46-47 0.7235399068861739 1101 48-49 0.7441606534219574 1101 50-51 0.6679615649221446 1101 52-53 0.7463088339382651 1101 54-55 0.7939593334485195 1101 56-57 0.7309213733100748 1101 58-59 0.6639339695001496 1101 60-61 0.7287916156601213 1101 62-63 0.9617650355075753 1101 64-65 0.8043448867111564 1101 66-67 0.8755393366285489 1101 68-69 0.9305906646601443 1101 70-71 0.9219269081890538 1101 72-73 0.9539759357130038 1101 74-75 1.0373050750371853 1101 76-77 1.0835706346892167 1101 78-79 1.0583912882736826 1101 80-81 1.019187914821913 1101 82-83 0.9936181600166876 1101 84-85 1.2157976258845196 1101 86-87 1.3518740119803674 1101 88-89 1.3519729009215702 1101 90-91 1.6596884741904283 1101 92-93 1.634750133094979 1101 94-95 1.8902128155710685 1101 96-97 2.1319669893968793 1101 98-99 2.219188972249796 1101 100-101 2.8283749949453387 1102 1 0.28902147381666765 1102 2 0.1823349642237737 1102 3 0.314691353636519 1102 4 0.25460620262671796 1102 5 0.2348904096135982 1102 6 0.2623436402426762 1102 7 0.16405270794543014 1102 8 0.33170042454342763 1102 9 0.2996862274467986 1102 10-11 0.3582411936647105 1102 12-13 0.42551198306879456 1102 14-15 0.5641616172315267 1102 16-17 0.46299630296357464 1102 18-19 0.5527433330300866 1102 20-21 0.5461218328968656 1102 22-23 0.6012422171760718 1102 24-25 0.6933566277821583 1102 26-27 0.5994746735526135 1102 28-29 0.38262533454656733 1102 30-31 0.5842172252557205 1102 32-33 0.5768537858719824 1102 34-35 0.7426361187574386 1102 36-37 0.7899472453080207 1102 38-39 0.7205462797579827 1102 40-41 0.8255996300474351 1102 42-43 0.8373919618394439 1102 44-45 0.9448209601139794 1102 46-47 0.8865904179343858 1102 48-49 0.6986193102983549 1102 50-51 0.6802191951024881 1102 52-53 0.7406790104112275 1102 54-55 0.8726274441242907 1102 56-57 0.8650256346701539 1102 58-59 0.8775181088163677 1102 60-61 0.979078953958286 1102 62-63 1.064187653484936 1102 64-65 0.8564110323637237 1102 66-67 0.9375002898418217 1102 68-69 1.028365273795039 1102 70-71 1.0104293341522244 1102 72-73 0.9825359547806158 1102 74-75 1.0976593590470145 1102 76-77 0.9871690886516689 1102 78-79 1.121421170620394 1102 80-81 1.1657052185391237 1102 82-83 1.2613275517905755 1102 84-85 1.295382459328664 1102 86-87 1.423927696782176 1102 88-89 1.6169167922473413 1102 90-91 1.6113964052292395 1102 92-93 1.5103831763720414 1102 94-95 1.7470284409761483 1102 96-97 2.0215585550456296 1102 98-99 2.3055755624787615 1102 100-101 2.8006048600085443 1103 1 0.4161042305092941 1103 2 0.3679100216328486 1103 3 0.34459803868151084 1103 4 0.3740699302455255 1103 5 0.27634734133331307 1103 6 0.29317359950344724 1103 7 0.37743281234579484 1103 8 0.42157931956305816 1103 9 0.4464139450066753 1103 10-11 0.4125111630511782 1103 12-13 0.45086057541639235 1103 14-15 0.6277252498523964 1103 16-17 0.5599394001744216 1103 18-19 0.5753335080460502 1103 20-21 0.40671604984543563 1103 22-23 0.5487180956287929 1103 24-25 0.5489197096097342 1103 26-27 0.32946592484092463 1103 28-29 0.2121662149534913 1103 30-31 0.517577912801535 1103 32-33 0.38502249552742285 1103 34-35 0.42622122675326324 1103 36-37 0.5310407369063412 1103 38-39 0.6640183360390495 1103 40-41 0.722564286086758 1103 42-43 0.7188172924892839 1103 44-45 0.6930235797202684 1103 46-47 0.6410208962761033 1103 48-49 0.7014080490790704 1103 50-51 0.7391035883188337 1103 52-53 0.7216187806080256 1103 54-55 0.685183444919879 1103 56-57 0.6390685270025855 1103 58-59 0.7872781381920078 1103 60-61 0.9027974206943981 1103 62-63 0.9974433286726736 1103 64-65 1.0773183669553532 1103 66-67 0.9170954845875912 1103 68-69 1.112491438841083 1103 70-71 1.0141378699183186 1103 72-73 1.2037083934179336 1103 74-75 1.0590461063483367 1103 76-77 1.1642971188841003 1103 78-79 1.120533225065472 1103 80-81 1.0267264393808055 1103 82-83 1.1568330228648875 1103 84-85 1.2957272614951165 1103 86-87 1.3479643996444928 1103 88-89 1.479351576791192 1103 90-91 1.4699009457966916 1103 92-93 1.6619769301535285 1103 94-95 1.9591802485100516 1103 96-97 2.133048715179047 1103 98-99 2.0504515853060816 1103 100-101 2.3577641296788876 1104 1 0.24781873109071384 1104 2 0.3454722704790534 1104 3 0.2735791621026422 1104 4 0.32307962097601006 1104 5 0.21148919539176347 1104 6 0.2632717203066477 1104 7 0.1791577612591908 1104 8 0.35479961571231655 1104 9 0.3537140837796713 1104 10-11 0.41620050413828125 1104 12-13 0.25693059983665023 1104 14-15 0.6034751834718222 1104 16-17 0.6872832471533954 1104 18-19 0.6120480949083102 1104 20-21 0.5340977490659569 1104 22-23 0.5384487268316249 1104 24-25 0.5969988753463298 1104 26-27 0.6743708183007797 1104 28-29 0.5197322378908282 1104 30-31 0.6146857510544521 1104 32-33 0.5176943238296516 1104 34-35 0.5748005987289062 1104 36-37 0.7218758742515305 1104 38-39 0.7096522748041423 1104 40-41 0.6955727154469642 1104 42-43 0.8980931624089408 1104 44-45 0.9389232913195897 1104 46-47 0.9963700859876354 1104 48-49 0.833442373269051 1104 50-51 0.845424133346576 1104 52-53 0.8341735341537557 1104 54-55 0.8232824775167842 1104 56-57 0.9937586423906879 1104 58-59 0.9005342715076026 1104 60-61 0.9100995634095952 1104 62-63 1.158198736950844 1104 64-65 0.9527517890373502 1104 66-67 0.9285794650179753 1104 68-69 0.8273126510145872 1104 70-71 0.8025267226558448 1104 72-73 0.9908347625696905 1104 74-75 0.8906238100421042 1104 76-77 1.0232008010722282 1104 78-79 1.1522248751393143 1104 80-81 0.9998242111640749 1104 82-83 1.086756834095759 1104 84-85 1.21271242508638 1104 86-87 1.4804489150161189 1104 88-89 1.3984535434934635 1104 90-91 1.5196373995649104 1104 92-93 1.5425707817248693 1104 94-95 1.829142490899109 1104 96-97 2.043470689741227 1104 98-99 2.2360059174672955 1104 100-101 2.631008467568037 1105 1 0.15083617481791478 1105 2 0.19094651058328083 1105 3 0.22783414954348302 1105 4 0.1652932321602094 1105 5 0.16052230822018743 1105 6 0.17909347877437654 1105 7 0.19009819711474307 1105 8 0.30576209819498246 1105 9 0.23053116284499708 1105 10-11 0.272659516769302 1105 12-13 0.34022152837984976 1105 14-15 0.4160124929255531 1105 16-17 0.3819652807750771 1105 18-19 0.4273838307289779 1105 20-21 0.4542042266246469 1105 22-23 0.45445101958810596 1105 24-25 0.3488914426656251 1105 26-27 0.3831139519123852 1105 28-29 0.39918662443937336 1105 30-31 0.35377777080136497 1105 32-33 0.3718562332391073 1105 34-35 0.4815084183267615 1105 36-37 0.37299669370912625 1105 38-39 0.3930342529035613 1105 40-41 0.5024485507440488 1105 42-43 0.656365284089631 1105 44-45 0.5620418806136485 1105 46-47 0.46005719311546756 1105 48-49 0.4743637298668659 1105 50-51 0.3218575987819392 1105 52-53 0.3593616897134524 1105 54-55 0.39264183869779856 1105 56-57 0.5224806914519675 1105 58-59 0.4841375852246159 1105 60-61 0.5096890000382928 1105 62-63 0.5211911782728578 1105 64-65 0.6024405050357586 1105 66-67 0.5854634721721226 1105 68-69 0.7183006575103406 1105 70-71 0.8391719956712116 1105 72-73 0.913880500627485 1105 74-75 0.8596521820343099 1105 76-77 0.9461793096677447 1105 78-79 1.0401817601896823 1105 80-81 1.1055845589954032 1105 82-83 1.1979375573224935 1105 84-85 1.226433900378975 1105 86-87 1.3195021619093659 1105 88-89 1.1127094930110921 1105 90-91 1.3346216296194946 1105 92-93 1.4842156084757043 1105 94-95 1.5344887979731539 1105 96-97 1.6763969564080483 1105 98-99 1.913238428244295 1105 100-101 1.9678735693337366 1106 1 0.10995220680945295 1106 2 0.04281142112184 1106 3 0.14918713762860136 1106 4 0.07097066964187349 1106 5 0.09380394751794086 1106 6 0.017734403096781648 1106 7 0.07641171914508504 1106 8 -0.06559550378940315 1106 9 0.11082595657615002 1106 10-11 -0.03369409797974754 1106 12-13 -0.11917753534292075 1106 14-15 -0.10087713296296386 1106 16-17 -0.2630757416099101 1106 18-19 0.0701767823436441 1106 20-21 0.11929746182195089 1106 22-23 0.16385696972769637 1106 24-25 0.03496483534150485 1106 26-27 0.06800839002897874 1106 28-29 0.1221629475610726 1106 30-31 0.06566248667468955 1106 32-33 0.13428518263359024 1106 34-35 0.053474917286223445 1106 36-37 0.12929466621029206 1106 38-39 0.1985219007377168 1106 40-41 0.22141904520254485 1106 42-43 0.10802573691285744 1106 44-45 0.2894096717403585 1106 46-47 0.47236838478235654 1106 48-49 0.5918559860911543 1106 50-51 0.47152663758965474 1106 52-53 0.732941882725946 1106 54-55 0.5979916893314154 1106 56-57 0.5124003545133675 1106 58-59 0.6202259214249892 1106 60-61 0.6614900734929492 1106 62-63 0.7913862842341501 1106 64-65 0.9146214056964439 1106 66-67 0.9486617031893658 1106 68-69 0.8505773620018005 1106 70-71 0.8341235635017306 1106 72-73 0.8229377914835609 1106 74-75 1.034783402706779 1106 76-77 0.8986892020055066 1106 78-79 0.9454532433999105 1106 80-81 1.1294649309170026 1106 82-83 0.8929343689115044 1106 84-85 1.1196042276352713 1106 86-87 1.2767554181515557 1106 88-89 1.4095219772584144 1106 90-91 1.5557134847788667 1106 92-93 1.7992543721159748 1106 94-95 1.7757155029466674 1106 96-97 1.9886078129818898 1106 98-99 2.1206714199654435 1106 100-101 2.560714174952775 1107 1 0.08429775016987051 1107 2 0.11775264488570869 1107 3 0.09110660640241619 1107 4 0.11395616405931719 1107 5 0.06218388901917393 1107 6 0.12753734536004657 1107 7 0.05454235736635127 1107 8 0.23825090514160507 1107 9 0.06585621432534339 1107 10-11 0.2329835321299285 1107 12-13 0.21784276326877716 1107 14-15 0.28657568024297575 1107 16-17 0.3803524864134644 1107 18-19 0.34683825152621495 1107 20-21 0.40333962911868326 1107 22-23 0.4568702111305285 1107 24-25 0.4152741533206026 1107 26-27 0.41877630476857775 1107 28-29 0.2819778007253788 1107 30-31 0.4159340010780923 1107 32-33 0.39941642587449167 1107 34-35 0.20671407329060543 1107 36-37 0.17370439308520247 1107 38-39 0.327092633217525 1107 40-41 0.37030835367999515 1107 42-43 0.33102250608094863 1107 44-45 0.3623087850549851 1107 46-47 0.43423353714037205 1107 48-49 0.23856005028008553 1107 50-51 0.35542106910806837 1107 52-53 0.3006702437995017 1107 54-55 0.3980675536500087 1107 56-57 0.45031850496699377 1107 58-59 0.37606023267036903 1107 60-61 0.5278785258743781 1107 62-63 0.6862678452118729 1107 64-65 0.6612897371822299 1107 66-67 0.5846212548522232 1107 68-69 0.5919896700231178 1107 70-71 0.6908665546808983 1107 72-73 0.9177763107961354 1107 74-75 0.7426763372414911 1107 76-77 0.9362017727433987 1107 78-79 0.9936061062797208 1107 80-81 0.9524777391961621 1107 82-83 1.0805753878890911 1107 84-85 1.0900648546630052 1107 86-87 1.3212301135776379 1107 88-89 1.369589327989786 1107 90-91 1.4142799943028486 1107 92-93 1.5241352292677277 1107 94-95 1.4045173475121118 1107 96-97 1.7020142765406234 1107 98-99 2.0769557061031847 1107 100-101 2.499451538231199 1108 1 0.2114463733368197 1108 2 0.2339847818989078 1108 3 0.2271960299904734 1108 4 0.23850401599889892 1108 5 0.25716467759656325 1108 6 0.19637380325508502 1108 7 0.20034657920417231 1108 8 0.191784196571696 1108 9 0.26252611896950384 1108 10-11 0.27003802884952677 1108 12-13 0.22266466584894573 1108 14-15 0.35814623785040567 1108 16-17 0.37941485817137277 1108 18-19 0.4277586855883513 1108 20-21 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-0.9751663685698482 2316 88-89 -0.9086026275294117 2316 90-91 -0.8053139571586065 2316 92-93 -0.8684156597137722 2316 94-95 -0.9966429504214602 2316 96-97 -0.6861718822052154 2316 98-99 -0.5052984498231581 2316 100-101 -0.22018185872943974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7180.0 3 1259.0 4 1287.0 5 1563.0 6 1733.0 7 2101.0 8 2472.0 9 3298.0 10 4088.0 11 5271.0 12 6845.0 13 9137.0 14 10341.0 15 12746.0 16 15861.0 17 20202.0 18 17681.0 19 13037.0 20 13168.0 21 13818.0 22 15155.0 23 16989.0 24 19345.0 25 22008.0 26 25315.0 27 30008.0 28 35552.0 29 43358.0 30 52206.0 31 64469.0 32 81590.0 33 105415.0 34 140434.0 35 192118.0 36 263818.0 37 332822.0 38 229383.0 39 23880.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86627341673689 18.975281432985458 14.52896436982779 22.62948078044986 2 15.119662084836321 19.760108749835954 41.109123028922724 24.011106136405004 3 19.331708992411823 24.438608769280947 29.638929749415173 26.590752488892054 4 12.360862147161091 16.266440619398313 37.2493682914714 34.123328941969206 5 13.478054529875674 37.86257011412087 33.86140174790609 14.797973608097362 6 32.120428807719186 35.99551009284718 17.28085394580272 14.603207153630915 7 28.871209786953212 33.05373898975858 20.05087523426189 18.02417598902632 8 26.5840307359034 35.21180044545257 19.233037966007664 18.971130852636367 9 26.609390870340967 16.976738161568907 20.42769573847783 35.98617522961229 10-11 25.267339129683897 26.921457830740085 26.67891779868206 21.132285240893957 12-13 26.042420523580777 24.14954146611037 27.709554008130727 22.09848400217812 14-15 22.671087169568576 25.462836043845975 26.333247348129984 25.53282943845546 16-17 21.934314565655214 29.193073666896197 26.720385484640406 22.15222628280818 18-19 21.80079274923167 27.244913102488066 29.565067227185526 21.38922692109474 20-21 22.707457628947754 26.241427314962202 29.599599626605944 21.4515154294841 22-23 22.347734294722297 26.199539826036006 29.583267127236297 21.869458752005404 24-25 22.120595648503823 26.406536929122748 29.761571720427526 21.7112957019459 26-27 21.943843303666462 26.772140679622886 29.59775565315905 21.686260363551597 28-29 21.586777903228942 27.01822256091637 29.779806224728368 21.61519331112632 30-31 21.720321942447985 26.589285926296895 29.710788223650052 21.979603907605064 32-33 21.566799882345638 26.642856950107536 29.66565653022179 22.124686637325038 34-35 22.256154405524967 26.699360646072606 29.395817958843363 21.64866698955906 36-37 22.207464997454583 26.387266808195736 29.42018675038626 21.98508144396342 38-39 21.778095644828717 26.76685773761814 29.555008011274868 21.900038606278283 40-41 22.009584480091593 26.735028835990683 29.429571282817157 21.825815401100567 42-43 21.98294934555878 26.663704197748306 29.200554289266474 22.15279216742644 44-45 21.953381833780973 26.88310654675104 29.03832326766932 22.125188351798666 46-47 22.193737558944097 27.13867450447568 28.51302503346843 22.154562903111795 48-49 22.270475832766028 27.19881989566967 28.344818412174693 22.185885859389607 50-51 22.137833869571917 27.382519764549752 28.27381008599876 22.20583627987957 52-53 22.513419094626748 27.36164729912382 28.054328440707682 22.07060516554175 54-55 22.148124724113032 27.186265928593706 28.266825462437822 22.39878388485544 56-57 22.27208774894101 27.591182163642365 28.039917956552795 22.09681213086383 58-59 22.467884949030235 27.137218069450082 28.139781662969455 22.25511531855023 60-61 22.337884939559643 27.1457061598692 28.57824956441023 21.938159336160922 62-63 22.174284990499633 27.267037328901 28.488393581458865 22.0702840991405 64-65 22.068070700735735 27.29819876320918 28.36833977354724 22.265390762507838 66-67 21.976956264056362 27.49415894626069 28.124677126835508 22.404207662847444 68-69 22.011274884179794 27.26631403765435 28.389755863714022 22.332655214451837 70-71 22.06634497314305 27.348823106687586 28.30848157347841 22.27635034669095 72-73 22.23646262900329 27.2152337523358 28.250928856084702 22.29737476257621 74-75 21.983180482583215 27.634207059082506 28.00360086800666 22.379011590327618 76-77 22.110398606924957 27.431938792331934 28.019527657120936 22.438134943622174 78-79 22.167223311407522 27.538453817374116 27.966608870591298 22.32771400062707 80-81 21.985246546492913 27.785697751153236 27.964544659386885 22.264511042966966 82-83 22.26222330858266 27.68152516372297 27.84456016701173 22.211691360682636 84-85 22.092623616405394 27.602803473587944 27.76140260006528 22.54317030994138 86-87 22.200867924507943 27.478502870690257 27.958785725809022 22.361843478992785 88-89 22.189421349986414 27.536913796447955 27.724932760759962 22.548732092805665 90-91 22.088182652580553 27.57333400961653 27.905018542929866 22.433464794873053 92-93 22.40334964631231 27.37370031241063 27.89686672098331 22.32608332029374 94-95 22.15324698028729 27.26922250963405 27.90850699446723 22.669023515611425 96-97 22.245969369147737 27.208295837838186 27.905280444723545 22.64045434829053 98-99 22.09832743384557 27.36294920253727 27.619827134802104 22.918896228815054 100-101 22.335572005031416 26.808545419085327 28.201274901817026 22.65460767406623 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2523.0 1 1456.0 2 428.0 3 470.5 4 394.5 5 350.0 6 619.5 7 1296.5 8 2167.0 9 2540.0 10 2238.0 11 2000.0 12 2102.0 13 2329.0 14 2529.5 15 2598.5 16 2635.0 17 2726.0 18 2930.5 19 3140.0 20 3453.0 21 4043.5 22 4797.0 23 5703.0 24 6866.0 25 8499.0 26 10937.5 27 13690.0 28 15702.0 29 18250.0 30 21911.0 31 25265.5 32 28983.0 33 33014.5 34 37057.5 35 40890.5 36 45588.5 37 49997.5 38 52218.0 39 53286.5 40 54579.5 41 58037.0 42 61313.5 43 64456.0 44 67636.0 45 70190.0 46 70632.0 47 71583.0 48 76307.5 49 75576.0 50 70404.5 51 66181.5 52 62379.5 53 60641.5 54 58865.0 55 54373.0 56 48999.0 57 43931.5 58 39319.5 59 34801.0 60 29159.5 61 23399.5 62 18575.0 63 14240.0 64 10778.0 65 8534.0 66 6395.0 67 4923.0 68 3691.5 69 2461.5 70 1818.5 71 1413.0 72 1175.0 73 990.0 74 777.0 75 627.0 76 465.0 77 296.0 78 204.5 79 150.0 80 97.5 81 61.0 82 38.5 83 30.0 84 19.5 85 13.0 86 10.5 87 7.5 88 5.5 89 4.5 90 4.0 91 3.5 92 3.0 93 3.5 94 1.5 95 0.5 96 1.0 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30770821463536524 2 0.2889139957155643 3 0.27604345611146 4 0.2603187801097927 5 0.25821856653422753 6 0.21050602222779888 7 0.2042592331312461 8 0.21578348198178307 9 0.21799139881763363 10-11 0.21885302489991673 12-13 0.21449104285835838 14-15 0.2112060934196539 16-17 0.21055987385794156 18-19 0.21050602222779888 20-21 0.19838940544569225 22-23 0.1898000704379322 24-25 0.19093095467092883 26-27 0.20272446167217928 28-29 0.2200377607630561 30-31 0.21282164232393477 32-33 0.21912228305063022 34-35 0.2106406513031556 36-37 0.19518523345220185 38-39 0.1954006399727726 40-41 0.1906886223352867 42-43 0.20302064563796413 44-45 0.1996010671239029 46-47 0.20005880598011583 48-49 0.20988672848115783 50-51 0.21963387353698582 52-53 0.2353316237235817 54-55 0.20679025974795284 56-57 0.17781808273118235 58-59 0.17803348925175314 60-61 0.1848457204648042 62-63 0.1941082008493479 64-65 0.18129151287538625 66-67 0.1873228954513682 68-69 0.19076939978050075 70-71 0.18471109138944744 72-73 0.18656897262937047 74-75 0.18912692506114853 76-77 0.2039630491654613 78-79 0.20932128636465952 80-81 0.19887407011697653 82-83 0.19768933425383717 84-85 0.19058091907500133 86-87 0.1920887647189968 88-89 0.19836247963062092 90-91 0.19855096033612032 92-93 0.1951583076371305 94-95 0.19558912067827205 96-97 0.18298783922488118 98-99 0.19087710304078614 100-101 0.18306861667009522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1856954.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.59459907251127 #Duplication Level Percentage of deduplicated Percentage of total 1 75.99286889397614 39.96814471848589 2 13.270429247503248 13.959058115851219 3 4.449688968964587 7.020888219602056 4 1.9914716275172921 4.18962607254214 5 1.105558037387918 2.907319086390499 6 0.6885234369597419 2.172756847135507 7 0.4420611428675379 1.6275020002258 8 0.3105285631458158 1.3065700223373746 9 0.22600462647154948 1.0697960446023433 >10 1.3430750618808762 13.194753774882168 >50 0.11442006329184722 4.227752751976395 >100 0.061060869435129346 5.659994685604652 >500 0.0034886109605493467 1.1999728999270012 >1k 6.156372283322377E-4 0.7091582963635872 >5k 2.0521240944407923E-4 0.7867064640733865 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8357 0.45003807310251087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6221 0.3350109911177121 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4252 0.2289771313667436 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2638 0.14206060031643217 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2192 0.11804277327278974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.385163014269605E-5 0.0 0.0 0.0 0.0 3 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 4 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 5 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 6 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 7 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 8 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 9 5.385163014269605E-5 0.0 0.0 5.385163014269605E-5 0.0 10-11 5.385163014269605E-5 0.0 0.0 8.077744521404407E-5 0.0 12-13 1.077032602853921E-4 0.0 0.0 1.077032602853921E-4 0.0 14-15 1.3462907535674012E-4 0.0 0.0 1.077032602853921E-4 0.0 16-17 1.6155489042808815E-4 0.0 0.0 1.077032602853921E-4 0.0 18-19 1.6155489042808815E-4 0.0 0.0 1.3462907535674012E-4 0.0 20-21 1.6155489042808815E-4 0.0 0.0 2.154065205707842E-4 0.0 22-23 1.6155489042808815E-4 0.0 0.0 3.231097808561763E-4 0.0 24-25 1.6155489042808815E-4 0.0 0.0 4.8466467128426444E-4 0.0 26-27 1.6155489042808815E-4 0.0 0.0 5.654421164983086E-4 0.0 28-29 2.4233233564213222E-4 0.0 0.0 0.0012385874932820092 0.0 30-31 2.961839657848283E-4 0.0 0.0 0.0032041719934904154 0.0 32-33 3.231097808561763E-4 0.0 0.0 0.00586982768555387 0.0 34-35 3.500355959275243E-4 0.0 0.0 0.009881774131184726 0.0 36-37 3.7696141099887234E-4 0.0 0.0 0.014620717583741977 0.0 38-39 3.7696141099887234E-4 0.0 0.0 0.019521215926727318 0.0 40-41 3.7696141099887234E-4 0.0 0.0 0.024260159379284572 0.0 42-43 4.308130411415684E-4 0.0 0.0 0.03021076451005248 0.0 44-45 4.8466467128426444E-4 0.0 0.0 0.036726811757318706 0.0 46-47 4.8466467128426444E-4 0.0 0.0 0.04639317936793265 0.0 48-49 4.8466467128426444E-4 0.0 0.0 0.05934449641725105 0.0 50-51 4.8466467128426444E-4 0.0 0.0 0.07197270368571326 0.0 52-53 4.8466467128426444E-4 0.0 0.0 0.09340565248250629 0.0 54-55 4.8466467128426444E-4 0.0 0.0 0.1280053248491885 0.0 56-57 4.8466467128426444E-4 0.0 0.0 0.17647179197761498 0.0 58-59 4.8466467128426444E-4 0.0 0.0 0.2227034164551195 0.0 60-61 4.8466467128426444E-4 0.0 0.0 0.27020055424097744 0.0 62-63 4.8466467128426444E-4 0.0 0.0 0.3232174841164617 0.0 64-65 4.8466467128426444E-4 0.0 0.0 0.3856584492669177 0.0 66-67 5.923679315696566E-4 0.0 0.0 0.4684284047962416 0.0 68-69 5.923679315696566E-4 0.0 0.0 0.5739237482457831 0.0 70-71 5.923679315696566E-4 0.0 0.0 0.6808730857091775 0.0 72-73 6.192937466410046E-4 0.0 0.0 0.7896803044124949 0.0 74-75 6.462195617123526E-4 0.0 0.0 0.9073461162742857 0.0 76-77 6.462195617123526E-4 0.0 0.0 1.0382325033361084 0.0 78-79 6.462195617123526E-4 0.0 0.0 1.1748810148231996 0.0 80-81 7.000711918550487E-4 0.0 0.0 1.3263925762296749 0.0 82-83 7.000711918550487E-4 0.0 0.0 1.4843932590683453 0.0 84-85 7.000711918550487E-4 0.0 0.0 1.6705852702867168 0.0 86-87 7.000711918550487E-4 0.0 0.0 1.873309731958896 0.0 88-89 7.000711918550487E-4 0.0 0.0 2.106164180695914 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10590 0.0 34.660557 1 GGTATCA 6840 0.0 30.51565 1 ATCAACG 12800 0.0 28.2531 3 TCAACGC 12885 0.0 28.029837 4 CAACGCA 12935 0.0 27.921486 5 TATCAAC 13030 0.0 27.905544 2 AACGCAG 13125 0.0 27.578537 6 GTACATG 9465 0.0 25.217226 1 ACGCAGA 14635 0.0 24.538311 7 TACCGTC 195 1.5703336E-8 24.360207 7 CGCAGAG 14780 0.0 24.329716 8 ATACCGT 215 1.6625563E-9 24.303555 6 TACATGG 9645 0.0 24.19651 2 GAGTACT 7030 0.0 23.411137 12-13 ACCGTCG 165 2.4301498E-6 23.03147 8 ACATGGG 9715 0.0 22.94148 3 GCAGAGT 15525 0.0 22.916803 9 CATGGGG 3855 0.0 22.682182 4 AGTACTT 7255 0.0 22.554146 12-13 GTACTTT 7545 0.0 21.655483 14-15 >>END_MODULE