FastQCFastQC Report
Thu 26 May 2016
SRR937601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856954
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90180.48563400062683293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75000.4038872260702204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.28789081474285305No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25880.13936801880929736No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.10059484510655622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72800.033.284621
GTATCAA107550.031.586421
ATCAACG133200.025.2844333
TCAACGC133850.025.2326264
CAACGCA134600.025.0567365
GTACATG87500.024.9778211
TATCAAC136300.024.813922
AACGCAG137850.024.497816
TACATGG91000.023.6987632
ACATGGG90850.022.7444553
CGTAAGC1503.0335603E-522.1675662
CGGCTCG1302.9405757E-421.9251448
GAGTACT82300.021.64542212-13
ACGCAGA156900.021.2846747
CGCAGAG157800.021.1331738
CATGGGG36800.020.7820914
AGTACTT86000.020.74178312-13
GTACTTT87400.020.48775314-15
AGAGTAC132500.020.2028510-11
TAGTCGG1803.6379788E-1219.79726640-41
Produced by FastQC (version 0.11.5)