##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856954 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06261544443212 31.0 31.0 34.0 30.0 34.0 2 32.27444675527773 33.0 31.0 34.0 30.0 34.0 3 32.339227035241585 34.0 31.0 34.0 30.0 34.0 4 35.991688539403775 37.0 35.0 37.0 35.0 37.0 5 35.90003306490091 37.0 35.0 37.0 35.0 37.0 6 35.94683336259272 37.0 35.0 37.0 35.0 37.0 7 35.89620959916078 37.0 35.0 37.0 35.0 37.0 8 35.85936808343126 37.0 35.0 37.0 35.0 37.0 9 37.52103606228264 39.0 37.0 39.0 35.0 39.0 10-11 37.4272962604351 39.0 37.0 39.0 34.0 39.0 12-13 37.31445986276451 39.0 37.0 39.0 34.0 39.0 14-15 38.482201766979685 40.0 38.0 41.0 34.0 41.0 16-17 38.43272315846273 40.0 38.0 41.0 34.0 41.0 18-19 38.408851807853075 40.0 38.0 41.0 34.0 41.0 20-21 38.30183354030309 40.0 38.0 41.0 34.0 41.0 22-23 38.24915506792306 40.0 38.0 41.0 33.5 41.0 24-25 38.26137050244648 40.0 38.0 41.0 34.0 41.0 26-27 38.07823941788542 40.0 37.5 41.0 33.0 41.0 28-29 38.033713274534534 40.0 37.5 41.0 33.0 41.0 30-31 38.042139169844816 40.0 37.0 41.0 33.0 41.0 32-33 37.90883322904068 40.0 37.0 41.0 33.0 41.0 34-35 37.750795927093506 40.0 37.0 41.0 33.0 41.0 36-37 37.558029708867316 40.0 37.0 41.0 33.0 41.0 38-39 37.40295451583615 40.0 36.0 41.0 32.5 41.0 40-41 37.21393071664673 39.5 36.0 41.0 32.0 41.0 42-43 36.971208764460506 39.0 36.0 41.0 31.0 41.0 44-45 36.63820724692157 39.0 35.5 41.0 30.5 41.0 46-47 36.274776865770505 39.0 35.0 40.5 30.0 41.0 48-49 35.92430076350841 39.0 35.0 40.0 29.0 41.0 50-51 35.67225386304669 39.0 35.0 40.0 28.0 41.0 52-53 35.39619640551139 38.5 35.0 40.0 27.5 41.0 54-55 35.150540616515 38.0 34.0 40.0 27.0 41.0 56-57 34.851256950899156 38.0 34.0 40.0 26.0 41.0 58-59 34.57459904768777 38.0 34.0 40.0 26.0 41.0 60-61 34.207963956026916 37.5 33.5 40.0 25.5 41.0 62-63 33.782276243784175 37.0 33.0 39.5 24.5 41.0 64-65 33.3632532092879 36.0 32.5 39.0 23.0 40.0 66-67 32.92374205284568 36.0 32.0 39.0 22.0 40.0 68-69 32.43274523763109 35.0 31.0 38.5 21.5 40.0 70-71 31.986676568186397 35.0 31.0 38.0 20.0 40.0 72-73 31.497774850642504 35.0 31.0 37.0 18.5 39.0 74-75 30.996973269127828 34.0 30.0 36.5 18.0 39.0 76-77 29.76388402728339 33.5 28.5 35.0 16.5 37.5 78-79 30.395770169858814 34.0 30.0 36.0 16.5 37.5 80-81 30.21993705821469 34.0 30.0 35.0 13.0 37.0 82-83 29.81083080140919 34.0 29.5 35.0 8.5 37.0 84-85 29.367901412743667 34.0 29.0 35.0 7.0 36.0 86-87 28.981780916490123 34.0 29.0 35.0 2.0 36.0 88-89 28.52250001884807 33.5 29.0 35.0 2.0 35.5 90-91 27.950723335096075 33.0 27.0 35.0 2.0 35.0 92-93 27.41427224368509 33.0 26.0 35.0 2.0 35.0 94-95 26.831139058910452 32.0 25.0 34.0 2.0 35.0 96-97 26.195555732667586 32.0 25.0 34.0 2.0 35.0 98-99 25.232154108287013 32.0 21.5 34.0 2.0 35.0 100-101 22.664164540424803 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26716894326897744 1101 2 0.1906363120293193 1101 3 0.25205523951339615 1101 4 0.13661963341217387 1101 5 0.10308703604400193 1101 6 0.13642883946546647 1101 7 0.06835109011723262 1101 8 0.15264701516168344 1101 9 0.17686840922614522 1101 10-11 0.2967938056316939 1101 12-13 0.22499354989209053 1101 14-15 0.29339800339322863 1101 16-17 0.33867723952656803 1101 18-19 0.3347504701178181 1101 20-21 0.1771453721889671 1101 22-23 0.21398474337135553 1101 24-25 0.2048548014084517 1101 26-27 0.06869721463923639 1101 28-29 0.24111115570381259 1101 30-31 0.2714275090221534 1101 32-33 0.30896574007678623 1101 34-35 0.29280487188284354 1101 36-37 0.24582748283334155 1101 38-39 0.21441795298676425 1101 40-41 0.2084117410482449 1101 42-43 0.2621153526472071 1101 44-45 0.2983085769094984 1101 46-47 0.36367802241441183 1101 48-49 0.31626758068246374 1101 50-51 0.4133332382673771 1101 52-53 0.364157414881916 1101 54-55 0.3483030505231497 1101 56-57 0.5206414765834069 1101 58-59 0.4544796321801883 1101 60-61 0.5107584973475809 1101 62-63 0.4941027763207373 1101 64-65 0.6685200965661622 1101 66-67 0.640875261752754 1101 68-69 0.5755441328770274 1101 70-71 0.37882219911122306 1101 72-73 0.5659452608213975 1101 74-75 0.5702377452031655 1101 76-77 0.5302100387064321 1101 78-79 0.6508207317887766 1101 80-81 0.6529257666975603 1101 82-83 0.7540318350324604 1101 84-85 0.745705951679394 1101 86-87 0.6595728220996584 1101 88-89 0.8017573130953579 1101 90-91 0.8515589065932865 1101 92-93 0.9791101007109226 1101 94-95 1.2107258007254593 1101 96-97 1.2314196206522006 1101 98-99 1.3565314319701507 1101 100-101 1.6208945394433378 1102 1 0.29163207434346816 1102 2 0.28936304724459916 1102 3 0.29524031102390325 1102 4 0.22356096845155804 1102 5 0.19068207790772362 1102 6 0.2323824081611363 1102 7 0.21878366562454943 1102 8 0.28192736618412795 1102 9 0.23313124118445927 1102 10-11 0.29868049551958364 1102 12-13 0.21387802498223607 1102 14-15 0.31845044629145036 1102 16-17 0.3418318976406809 1102 18-19 0.4577180203340774 1102 20-21 0.4724244659756849 1102 22-23 0.4280182538606283 1102 24-25 0.4130830050248804 1102 26-27 0.4685589465859863 1102 28-29 0.5131287402779989 1102 30-31 0.3861548686738061 1102 32-33 0.33883303340995496 1102 34-35 0.41810485919897644 1102 36-37 0.5505329175204992 1102 38-39 0.4829127686316781 1102 40-41 0.6282993425017267 1102 42-43 0.5014754726281936 1102 44-45 0.6171455084180266 1102 46-47 0.5139650819463313 1102 48-49 0.5131360601659694 1102 50-51 0.5330730936155774 1102 52-53 0.5871439212022409 1102 54-55 0.622496266189259 1102 56-57 0.5791186881405039 1102 58-59 0.6692673447220159 1102 60-61 0.690759668165775 1102 62-63 0.7550603104661135 1102 64-65 0.7497877023962829 1102 66-67 0.6374153546097858 1102 68-69 0.6135686838194658 1102 70-71 0.5019582635173769 1102 72-73 0.6408458458123469 1102 74-75 0.5725792422057339 1102 76-77 0.48428873720262544 1102 78-79 0.42652932071590044 1102 80-81 0.5968983528258747 1102 82-83 0.6901313657295027 1102 84-85 0.7358472443463135 1102 86-87 0.5860947812130171 1102 88-89 0.6446979066165532 1102 90-91 0.9446598603919654 1102 92-93 1.1652586245740117 1102 94-95 1.2233564201300986 1102 96-97 1.2605409355367847 1102 98-99 1.1182254665120723 1102 100-101 1.3755101417108264 1103 1 0.24856866217900375 1103 2 0.22393854000291924 1103 3 0.23237754896243246 1103 4 0.1843305769015089 1103 5 0.2137803458297185 1103 6 0.2643487263165909 1103 7 0.1801800100817772 1103 8 0.22696042073631162 1103 9 0.2233819216784525 1103 10-11 0.4207529804736154 1103 12-13 0.4320953741365017 1103 14-15 0.40644426516669085 1103 16-17 0.36211221804660454 1103 18-19 0.3806371413458649 1103 20-21 0.4001858389531563 1103 22-23 0.42863261092767857 1103 24-25 0.2647835793242521 1103 26-27 0.32013420150174454 1103 28-29 0.3670714327392304 1103 30-31 0.3298443213994702 1103 32-33 0.32093044315148234 1103 34-35 0.358375220817031 1103 36-37 0.40862853579292135 1103 38-39 0.3868456553031763 1103 40-41 0.47402798222310594 1103 42-43 0.4348485391142276 1103 44-45 0.5291425096545339 1103 46-47 0.4646732546895791 1103 48-49 0.5361576899299436 1103 50-51 0.6153965233330965 1103 52-53 0.6101346731602675 1103 54-55 0.5566279252593418 1103 56-57 0.5217635808540351 1103 58-59 0.5617782249759316 1103 60-61 0.6713828713069674 1103 62-63 0.45748129735974175 1103 64-65 0.5752171055783606 1103 66-67 0.5145636918677496 1103 68-69 0.5974803732944451 1103 70-71 0.541545279817246 1103 72-73 0.44305291349176557 1103 74-75 0.6853533348920067 1103 76-77 0.5610262049165442 1103 78-79 0.7722944018118696 1103 80-81 0.7592284031900824 1103 82-83 0.8691173497092066 1103 84-85 0.9482022612247896 1103 86-87 0.8659609217515438 1103 88-89 1.1044694108525164 1103 90-91 1.276099030418795 1103 92-93 1.445637726141129 1103 94-95 1.4955808600525984 1103 96-97 1.5713615201843965 1103 98-99 1.6832685911234933 1103 100-101 1.9775066283172258 1104 1 0.2596076004458965 1104 2 0.2637849823653724 1104 3 0.24066023172891704 1104 4 0.19357682187564507 1104 5 0.17489078586758922 1104 6 0.18390297164030756 1104 7 0.19825334211996903 1104 8 0.19488203765047274 1104 9 0.20448669501310945 1104 10-11 0.3077693276321085 1104 12-13 0.2563916703503324 1104 14-15 0.328634111970878 1104 16-17 0.40031347952821505 1104 18-19 0.4180460106827084 1104 20-21 0.41527997692123364 1104 22-23 0.2657517714540347 1104 24-25 0.3041400299863639 1104 26-27 0.3244358582609621 1104 28-29 0.307761980088209 1104 30-31 0.41620267970321834 1104 32-33 0.41472036543748914 1104 34-35 0.364331660680115 1104 36-37 0.34678228714489023 1104 38-39 0.4482610563782998 1104 40-41 0.4948919429369383 1104 42-43 0.579852265890068 1104 44-45 0.6420773164049223 1104 46-47 0.6540143417948272 1104 48-49 0.7852659356511111 1104 50-51 0.7456740581947301 1104 52-53 0.757916503973 1104 54-55 0.904822623830249 1104 56-57 0.7443252980989001 1104 58-59 0.7828911198430077 1104 60-61 0.8337791464237299 1104 62-63 0.7415986916336905 1104 64-65 0.8504108339072616 1104 66-67 0.9024759681846604 1104 68-69 0.9211239966668785 1104 70-71 0.9303910592088123 1104 72-73 0.7029401416444792 1104 74-75 0.8761873589359723 1104 76-77 0.7885202640628961 1104 78-79 0.6576760052884865 1104 80-81 0.8111433091244784 1104 82-83 0.7582315738579339 1104 84-85 0.8053199299274958 1104 86-87 0.8598788180130228 1104 88-89 1.0265124410580455 1104 90-91 0.9567400859619504 1104 92-93 1.0919008749243737 1104 94-95 1.1402229030939388 1104 96-97 1.426030204523979 1104 98-99 1.6157474286588922 1104 100-101 1.9721343932703945 1105 1 0.1968089074793724 1105 2 0.20732440491065063 1105 3 0.12270961756460252 1105 4 0.09970362823277412 1105 5 0.14165045329039572 1105 6 0.19842859244064215 1105 7 0.2042043086593992 1105 8 0.12327686469109267 1105 9 0.23178533408014346 1105 10-11 0.16648516376376676 1105 12-13 0.06652436739930323 1105 14-15 0.0912352894137669 1105 16-17 0.17788045687999698 1105 18-19 0.2784472198857273 1105 20-21 0.305897931592348 1105 22-23 0.21627929090711007 1105 24-25 0.20524896837519435 1105 26-27 0.27440612661084174 1105 28-29 0.27250360451429145 1105 30-31 0.2887182323120143 1105 32-33 0.16859938949752262 1105 34-35 0.4072431832303849 1105 36-37 0.35269007614174797 1105 38-39 0.42445226392375446 1105 40-41 0.4026276198738614 1105 42-43 0.4740507689913116 1105 44-45 0.5079916312961359 1105 46-47 0.6012267532808622 1105 48-49 0.7325218144618901 1105 50-51 0.7433555110929646 1105 52-53 0.7259248427445755 1105 54-55 0.7816657832904141 1105 56-57 0.7203351020091091 1105 58-59 0.8225880888374704 1105 60-61 0.752592326180249 1105 62-63 0.6539459619436556 1105 64-65 0.7650760717546632 1105 66-67 0.8774321241431053 1105 68-69 0.6633560959531977 1105 70-71 0.7220324665301057 1105 72-73 0.6711869711378178 1105 74-75 0.8955569784056259 1105 76-77 0.9251006838297471 1105 78-79 0.9898130212508462 1105 80-81 0.9607491474237122 1105 82-83 0.9671187292055166 1105 84-85 1.1142404681804798 1105 86-87 1.1862201906763623 1105 88-89 1.1265536480819023 1105 90-91 1.199180248842712 1105 92-93 1.1835960658154754 1105 94-95 1.4812014626911143 1105 96-97 1.5584977460236793 1105 98-99 1.9658552855806875 1105 100-101 2.0980470621989085 1106 1 -0.21838073755338883 1106 2 -0.16728771766205597 1106 3 -0.20022752184539883 1106 4 -0.08495977059956772 1106 5 -0.12081155090481133 1106 6 -0.21732642741289965 1106 7 -0.18097433187928402 1106 8 -0.09449642620354837 1106 9 -0.20042202419395494 1106 10-11 -0.5002601228282444 1106 12-13 -0.48795751925853637 1106 14-15 -0.3906796027380537 1106 16-17 -0.21023902796093097 1106 18-19 -0.2937089998570812 1106 20-21 -0.1183724869778402 1106 22-23 -0.22546231145190632 1106 24-25 -0.23606154429099035 1106 26-27 -0.17241214222661227 1106 28-29 -0.30139935485447467 1106 30-31 -0.3819901871589124 1106 32-33 -0.41435851934559764 1106 34-35 -0.737025527508429 1106 36-37 -0.4915071535904971 1106 38-39 -0.5147241481704441 1106 40-41 -0.6698550160261263 1106 42-43 -0.8331295600967721 1106 44-45 -0.6855683294237451 1106 46-47 -0.6351925441118027 1106 48-49 -0.7869836168550535 1106 50-51 -0.8320245846833245 1106 52-53 -0.7485368804848207 1106 54-55 -0.7127844533957344 1106 56-57 -0.936065064917635 1106 58-59 -0.7138992463533569 1106 60-61 -0.8346189780106883 1106 62-63 -0.7994491792760883 1106 64-65 -1.1707621497868672 1106 66-67 -1.079042966998621 1106 68-69 -0.8460056433904164 1106 70-71 -0.821776851342765 1106 72-73 -0.6768546007832335 1106 74-75 -0.9116329916660071 1106 76-77 -0.7455932741658629 1106 78-79 -0.7460942710513763 1106 80-81 -0.8235758528861759 1106 82-83 -0.9707719888687123 1106 84-85 -0.6104757081503536 1106 86-87 -0.4517743056993133 1106 88-89 -0.7519926844190934 1106 90-91 -0.976378962635021 1106 92-93 -0.9202576419002035 1106 94-95 -0.7568313221620748 1106 96-97 -1.055996508638767 1106 98-99 -1.1162252937597898 1106 100-101 -0.37486246267964773 1107 1 0.06356923936924375 1107 2 0.12418556605154407 1107 3 0.05826001028358263 1107 4 0.07394450771487726 1107 5 0.1452360196097544 1107 6 0.08803388411130442 1107 7 -0.05751716578041055 1107 8 0.0190266423694041 1107 9 0.09593405219282403 1107 10-11 0.035079723602343904 1107 12-13 0.08812539144083331 1107 14-15 0.07077103672844487 1107 16-17 0.17507286845754066 1107 18-19 0.20357010368519468 1107 20-21 0.16498209648577244 1107 22-23 0.06224618007308891 1107 24-25 -0.09196434321739133 1107 26-27 -0.006486523096626229 1107 28-29 0.03144028516561548 1107 30-31 0.027380714239122028 1107 32-33 0.05860373527968221 1107 34-35 0.11756335014631247 1107 36-37 0.30266266337414294 1107 38-39 0.2607746546486567 1107 40-41 0.08697344940316043 1107 42-43 0.1086877185601125 1107 44-45 0.14872836570726378 1107 46-47 -0.019268346168864525 1107 48-49 -0.07776288755599836 1107 50-51 -0.18439438026248212 1107 52-53 -0.11880314326366204 1107 54-55 -0.08205013281112628 1107 56-57 -0.08784242852090074 1107 58-59 -0.0754248703559881 1107 60-61 -0.012026435046855966 1107 62-63 -0.055807299465207905 1107 64-65 -0.1678020201746122 1107 66-67 0.03656146385657166 1107 68-69 0.3122743277527178 1107 70-71 0.09716879028258418 1107 72-73 0.005123326151508678 1107 74-75 0.32925922956815157 1107 76-77 0.18266791023789608 1107 78-79 0.2161542634340634 1107 80-81 0.3811366680424939 1107 82-83 0.25580211114766627 1107 84-85 0.3776120372920353 1107 86-87 0.682602652662613 1107 88-89 0.881982619410401 1107 90-91 0.712770883267698 1107 92-93 0.7658396972315735 1107 94-95 0.9964685388322998 1107 96-97 0.787727393967959 1107 98-99 0.9839592575308522 1107 100-101 1.7494365875613447 1108 1 0.13753191778266682 1108 2 0.17242017570210777 1108 3 0.0974118333341707 1108 4 0.05740831249426748 1108 5 0.021807607482749347 1108 6 0.0069563129060981055 1108 7 0.19791943306188386 1108 8 0.0719433328447252 1108 9 0.10227408138656813 1108 10-11 0.01541160763773064 1108 12-13 0.22005349448817668 1108 14-15 0.18922863394136158 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-0.453768551692626 2316 52-53 -0.6801872000788975 2316 54-55 -0.5222790539081146 2316 56-57 -0.5937956976095222 2316 58-59 -0.4824934180584606 2316 60-61 -0.4154771444655765 2316 62-63 -0.4097358996816425 2316 64-65 -0.4284625950841843 2316 66-67 -0.4287222093083187 2316 68-69 -0.43487959373015883 2316 70-71 -0.3535723563466284 2316 72-73 -0.12711770985880122 2316 74-75 -0.19251339971447123 2316 76-77 -0.22863331102905704 2316 78-79 -0.26910684004520036 2316 80-81 -0.2641299578066558 2316 82-83 -0.3491615046668919 2316 84-85 -0.369001652354207 2316 86-87 -0.046721474434217924 2316 88-89 0.05341747516849793 2316 90-91 -0.009214237204002274 2316 92-93 0.016071225161560676 2316 94-95 0.07595893728329273 2316 96-97 0.21310765715519508 2316 98-99 0.009032446082052559 2316 100-101 0.5851289332479759 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 20.0 5 43.0 6 97.0 7 258.0 8 619.0 9 1098.0 10 1741.0 11 2911.0 12 4303.0 13 6265.0 14 8324.0 15 11469.0 16 14822.0 17 19820.0 18 17744.0 19 11696.0 20 10392.0 21 10742.0 22 11145.0 23 12520.0 24 14675.0 25 17392.0 26 21218.0 27 26471.0 28 32904.0 29 40943.0 30 50776.0 31 64140.0 32 80390.0 33 104326.0 34 140454.0 35 198304.0 36 286938.0 37 382615.0 38 236246.0 39 13119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61937507306149 19.790035408899254 15.240102288784275 22.35048722925498 2 15.736738766819212 20.650861572230653 39.769482712011175 23.84291694893896 3 19.663545785194465 24.45650242278484 30.131279503961867 25.74867228805883 4 13.079303739628823 17.033801143704 36.78641685905213 33.100478257615045 5 13.552258548186957 38.00771017165311 33.82689705983731 14.613134220322625 6 32.20882154323694 36.442852111576265 16.810001755563142 14.538324589623652 7 28.863504427142516 33.333835948548 19.914171271878573 17.88848835243092 8 26.891188473166274 35.32494612144404 18.91743144956741 18.866433955822277 9 26.501841187234575 16.849636555348166 20.342076324992433 36.30644593242482 10-11 25.351031851085164 27.04703509079923 26.50738790513928 21.094545152976327 12-13 26.10606303357671 24.22539572694336 27.67329925263919 21.995241986840742 14-15 22.67560208815081 25.557714407572828 26.345779163081044 25.42090434119531 16-17 21.96626841591122 29.15904217336563 26.858958272525868 22.015731138197285 18-19 21.79106213724196 27.16984373333965 29.790991053090167 21.248103076328224 20-21 22.564016263227334 26.063195024098658 30.06726083093244 21.305527881741565 22-23 22.218314922051157 26.13810588807398 29.996262634476494 21.647316555398366 24-25 22.096077770370186 26.333985656079793 30.16192108151306 21.40801549203696 26-27 21.92758685312516 26.70448181081873 29.915026323045375 21.452905013010735 28-29 21.52474988926601 26.900942551607425 30.144795636878662 21.429511922247908 30-31 21.752288963539215 26.457548221442213 30.094983505245686 21.695179309772886 32-33 21.614051828962914 26.541879874245673 30.034965863451653 21.80910243333976 34-35 22.237653706015333 26.477338695519652 29.81040456575661 21.4746030327084 36-37 22.212340413501188 26.23741382026148 29.819529092771873 21.73071667346546 38-39 21.789419662522604 26.640212950886237 29.95122657857976 21.6191408080114 40-41 21.95756482403942 26.597565901074343 29.819031207086887 21.62583806779935 42-43 22.02682275164407 26.521905283084013 29.489326027128687 21.96194593814323 44-45 21.982504682399238 26.70981618284567 29.363651442092802 21.944027692662285 46-47 22.248289402968517 27.08007306589178 28.805236963328117 21.86640056781159 48-49 22.403974465711052 27.185299151190605 28.54599521582118 21.864731167277167 50-51 22.215682240917115 27.308027016285806 28.454878257619736 22.02141248517734 52-53 22.624739223481033 27.377980283841175 28.18112349578934 21.816156996888452 54-55 22.21460520831426 27.216748503193937 28.32805766863369 22.240588619858112 56-57 22.378852680249484 27.519663922746606 28.172399531706226 21.929083865297684 58-59 22.63023208975559 27.09542078048245 28.225335684136493 22.04901144562547 60-61 22.382460739469046 27.087262258515825 28.7733029466535 21.75697405536163 62-63 22.260055984154697 27.198008135904285 28.6235954148568 21.918340465084217 64-65 22.15741477710272 27.21112100784403 28.50991462362557 22.121549591427684 66-67 22.09871650024718 27.387969761232643 28.30460528370654 22.208708454813635 68-69 22.18062482969422 27.10306771196271 28.518046219777123 22.19826123856595 70-71 22.213124288485336 27.295398809017346 28.41737059722535 22.074106305271968 72-73 22.320746771325513 27.169897584969792 28.31973759177664 22.18961805192805 74-75 22.207658348025852 27.553348117400862 27.984565045768502 22.254428488804784 76-77 22.189887310078763 27.32003592980763 28.26717840075737 22.222898359356236 78-79 22.21735164145154 27.439532697094272 28.125548613482078 22.21756704797211 80-81 22.02504747021197 27.69772433781343 28.12772960450286 22.14949858747174 82-83 22.230422653078424 27.62171379550914 27.993075736887697 22.15478781452474 84-85 22.103643637050983 27.5613041711689 27.926015220123112 22.40903697165701 86-87 22.196268728250672 27.36171709153808 28.13373406126377 22.30828011894748 88-89 22.1203110039344 27.497827086723742 27.873953797455403 22.507908111886454 90-91 22.083287554369253 27.573100926997096 27.928453403477477 22.415158115156174 92-93 22.325260782698138 27.31675202352295 28.043120021971983 22.314867171806927 94-95 22.056227564064592 27.160769733660604 28.13556501668862 22.64743768558618 96-97 22.135362534559285 27.064806128746323 28.162383128499684 22.637448208194712 98-99 22.045973483257402 27.290003985050674 27.748311738666835 22.915710793025085 100-101 21.928703209747813 27.052892397736095 28.273738914156194 22.7446654783599 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 358.0 1 257.5 2 252.5 3 352.0 4 301.5 5 297.5 6 575.5 7 1329.0 8 2211.5 9 2606.5 10 2418.0 11 2128.5 12 2167.0 13 2389.5 14 2622.5 15 2809.0 16 2970.5 17 3110.0 18 3325.0 19 3560.5 20 3789.0 21 4261.0 22 5001.0 23 6025.0 24 7295.5 25 8854.5 26 11067.5 27 13709.0 28 15913.0 29 18386.5 30 21790.5 31 25197.5 32 29051.5 33 33015.0 34 37127.5 35 40932.5 36 45235.5 37 49683.5 38 52396.0 39 53427.0 40 54513.0 41 57955.0 42 61078.5 43 64717.5 44 68307.0 45 70662.0 46 70747.0 47 71791.0 48 77211.0 49 76128.5 50 70791.0 51 66451.0 52 62109.0 53 60526.5 54 58867.0 55 53902.5 56 48197.0 57 43554.0 58 39429.0 59 34794.0 60 29002.5 61 23255.0 62 18453.5 63 14124.0 64 10350.5 65 8153.5 66 6206.5 67 4765.0 68 3442.0 69 2148.5 70 1536.0 71 1194.0 72 1064.0 73 943.0 74 746.5 75 619.0 76 434.5 77 264.5 78 176.0 79 117.0 80 77.0 81 52.5 82 31.5 83 16.0 84 11.5 85 9.0 86 3.0 87 0.0 88 0.0 89 1.0 90 1.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03451889492146817 2 0.0 3 0.0 4 2.6925815071348025E-4 5 0.010016403206541465 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0046581660073432085 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.154065205707842E-4 22-23 0.0016694005344235776 24-25 0.0 26-27 0.0014001423837100974 28-29 0.0014270681987814454 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.5003559592752435E-4 38-39 0.0 40-41 2.154065205707842E-4 42-43 0.019305809406156532 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.9618396578482824E-4 84-85 2.4233233564213225E-4 86-87 0.0 88-89 0.0 90-91 0.011093435809395386 92-93 0.002369471726278626 94-95 0.0 96-97 0.0 98-99 7.539228219977447E-4 100-101 0.013489833350745361 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1856954.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.145016178949795 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84379756863221 38.278872375417386 2 13.801096156352441 14.117145724077748 3 4.665025350210547 7.157783910351881 4 2.1287277902938304 4.3549526910063205 5 1.194478671500759 3.054581548965839 6 0.7278486590086486 2.2335498864494547 7 0.47711637325149436 1.7081487240432673 8 0.3249750400821865 1.3296682946206628 9 0.2578969515982946 1.187112938579695 >10 1.3827061420395252 13.290528577468145 >50 0.1278626664771055 4.510026241504639 >100 0.06424270589568173 5.9885692077064485 >500 0.003486387842306471 1.2342928654216385 >1k 4.225924657341177E-4 0.37337318653694884 >5k 3.1694434930058823E-4 1.1813938278500504 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9018 0.48563400062683293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7500 0.4038872260702204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5346 0.28789081474285305 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2588 0.13936801880929736 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1868 0.10059484510655622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.077032602853921E-4 0.0 0.0 0.0 0.0 7 1.077032602853921E-4 0.0 0.0 0.0 0.0 8 1.077032602853921E-4 0.0 0.0 0.0 0.0 9 1.077032602853921E-4 0.0 0.0 0.0 0.0 10-11 1.077032602853921E-4 0.0 0.0 8.077744521404407E-5 0.0 12-13 1.077032602853921E-4 0.0 0.0 1.077032602853921E-4 0.0 14-15 1.077032602853921E-4 0.0 0.0 1.077032602853921E-4 0.0 16-17 1.3462907535674012E-4 0.0 0.0 1.3462907535674012E-4 0.0 18-19 1.6155489042808815E-4 0.0 0.0 1.8848070549943617E-4 0.0 20-21 1.6155489042808815E-4 0.0 0.0 2.6925815071348025E-4 0.0 22-23 1.6155489042808815E-4 0.0 0.0 3.7696141099887234E-4 0.0 24-25 1.6155489042808815E-4 0.0 0.0 5.115904863556125E-4 0.0 26-27 1.6155489042808815E-4 0.0 0.0 5.654421164983086E-4 0.0 28-29 2.6925815071348025E-4 0.0 0.0 0.0011847358631393132 0.0 30-31 3.231097808561763E-4 0.0 0.0 0.003150320363347719 0.0 32-33 3.231097808561763E-4 0.0 0.0 0.0059236793156965655 0.0 34-35 3.500355959275243E-4 0.0 0.0 0.009935625761327421 0.0 36-37 3.7696141099887234E-4 0.0 0.0 0.014755346659098719 0.0 38-39 3.7696141099887234E-4 0.0 0.0 0.019709696632226756 0.0 40-41 3.7696141099887234E-4 0.0 0.0 0.024502491714926702 0.0 42-43 3.7696141099887234E-4 0.0 0.0 0.030480022660765965 0.0 44-45 4.308130411415684E-4 0.0 0.0 0.03680758920253275 0.0 46-47 4.8466467128426444E-4 0.0 0.0 0.04658166007343208 0.0 48-49 4.8466467128426444E-4 0.0 0.0 0.05980223527346397 0.0 50-51 4.8466467128426444E-4 0.0 0.0 0.07229581346656945 0.0 52-53 4.8466467128426444E-4 0.0 0.0 0.09364798481814843 0.0 54-55 4.8466467128426444E-4 0.0 0.0 0.12859769278075817 0.0 56-57 5.385163014269605E-4 0.0 0.0 0.17711801153932732 0.0 58-59 5.385163014269605E-4 0.0 0.0 0.2233496360168319 0.0 60-61 5.385163014269605E-4 0.0 0.0 0.2708467738026898 0.0 62-63 5.385163014269605E-4 0.0 0.0 0.3238367778631027 0.0 64-65 5.385163014269605E-4 0.0 0.0 0.3862508171984874 0.0 66-67 5.385163014269605E-4 0.0 0.0 0.4687784403921691 0.0 68-69 5.385163014269605E-4 0.0 0.0 0.5745161161773528 0.0 70-71 6.462195617123526E-4 0.0 0.0 0.6819770441271028 0.0 72-73 6.462195617123526E-4 0.0 0.0 0.7909996693509909 0.0 74-75 6.731453767837006E-4 0.0 0.0 0.9098502170759211 0.0 76-77 7.000711918550487E-4 0.0 0.0 1.0425944853776667 0.0 78-79 7.000711918550487E-4 0.0 0.0 1.1797276615360424 0.0 80-81 8.077744521404408E-4 0.0 0.0 1.3321816264700148 0.0 82-83 8.077744521404408E-4 0.0 0.0 1.4909900837608254 0.0 84-85 8.077744521404408E-4 0.0 0.0 1.6788514955136207 0.0 86-87 8.077744521404408E-4 0.0 0.0 1.8835684675010798 0.0 88-89 8.077744521404408E-4 0.0 0.0 2.1177692069916647 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7280 0.0 33.28462 1 GTATCAA 10755 0.0 31.58642 1 ATCAACG 13320 0.0 25.284433 3 TCAACGC 13385 0.0 25.232626 4 CAACGCA 13460 0.0 25.056736 5 GTACATG 8750 0.0 24.977821 1 TATCAAC 13630 0.0 24.81392 2 AACGCAG 13785 0.0 24.49781 6 TACATGG 9100 0.0 23.698763 2 ACATGGG 9085 0.0 22.744455 3 CGTAAGC 150 3.0335603E-5 22.167566 2 CGGCTCG 130 2.9405757E-4 21.925144 8 GAGTACT 8230 0.0 21.645422 12-13 ACGCAGA 15690 0.0 21.284674 7 CGCAGAG 15780 0.0 21.133173 8 CATGGGG 3680 0.0 20.782091 4 AGTACTT 8600 0.0 20.741783 12-13 GTACTTT 8740 0.0 20.487753 14-15 AGAGTAC 13250 0.0 20.20285 10-11 TAGTCGG 180 3.6379788E-12 19.797266 40-41 >>END_MODULE