FastQCFastQC Report
Thu 26 May 2016
SRR937600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751156
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76680.43788217611680513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.34245949532765785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.22927711751551547No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23300.13305496483465779No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21610.12340419699901095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63450.034.4776081
GTATCAA95900.033.373931
ATCAACG115450.027.5886253
TCAACGC116800.027.2697494
CAACGCA117050.027.1685565
CTATGCG1056.903015E-527.1456559
TATCAAC116950.027.1128272
AACGCAG120850.026.4942466
GTACATG87650.025.229691
TACATGG90750.024.0968572
ACATGGG90850.023.2331033
ACGCAGA136050.023.1145467
CGCAGAG136450.023.08168
GAGTACT67500.022.90627712-13
CCTATAC4600.021.7025453
GTATACG1100.002858135421.6166421
GCAGAGT143650.021.4623589
GTACTTT72200.021.1842714-15
AGTACTT70750.021.08193612-13
CATGGGG35250.020.9036334
Produced by FastQC (version 0.11.5)