##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937600_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751156 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.725186676686715 33.0 31.0 34.0 30.0 34.0 2 31.82302661784558 33.0 31.0 34.0 30.0 34.0 3 31.868502292200123 33.0 31.0 34.0 30.0 34.0 4 35.500461980543136 37.0 35.0 37.0 33.0 37.0 5 35.50965019678429 37.0 35.0 37.0 33.0 37.0 6 35.57608402678002 37.0 35.0 37.0 33.0 37.0 7 35.49688662803314 37.0 35.0 37.0 33.0 37.0 8 35.49698599096825 37.0 35.0 37.0 33.0 37.0 9 37.05951668497838 39.0 37.0 39.0 33.0 39.0 10-11 36.96665402739676 39.0 37.0 39.0 33.0 39.0 12-13 36.85806861296195 39.0 37.0 39.0 33.0 39.0 14-15 37.95519502545747 40.0 38.0 41.0 33.0 41.0 16-17 37.861683368015186 40.0 38.0 41.0 33.0 41.0 18-19 37.783181795339765 40.0 38.0 41.0 33.0 41.0 20-21 37.79476471542227 40.0 37.5 41.0 32.5 41.0 22-23 37.80025680179264 40.0 38.0 41.0 33.0 41.0 24-25 37.717891781200535 40.0 37.0 41.0 32.5 41.0 26-27 37.615438887226496 40.0 37.0 41.0 32.0 41.0 28-29 37.46308952486243 40.0 37.0 41.0 32.0 41.0 30-31 37.21660748671164 39.0 37.0 41.0 31.5 41.0 32-33 37.067762380964346 39.0 36.0 41.0 31.0 41.0 34-35 37.02109577901683 39.0 36.0 41.0 31.0 41.0 36-37 36.841101249688776 39.0 36.0 41.0 31.0 41.0 38-39 36.66148532740658 39.0 36.0 41.0 30.0 41.0 40-41 36.40311771195713 39.0 35.5 41.0 30.0 41.0 42-43 36.11139441603147 39.0 35.0 40.0 30.0 41.0 44-45 35.83207692518542 39.0 35.0 40.0 28.5 41.0 46-47 35.43897459735169 38.5 35.0 40.0 27.0 41.0 48-49 35.050068640372416 38.0 34.5 40.0 25.5 41.0 50-51 34.17154553906106 37.5 33.5 39.5 24.0 40.5 52-53 34.29670029397724 38.0 33.5 39.5 24.0 40.5 54-55 34.54345643677662 38.0 34.0 40.0 24.0 41.0 56-57 34.28193947312518 38.0 34.0 40.0 23.5 41.0 58-59 33.96847682330986 38.0 33.5 40.0 22.5 41.0 60-61 33.58312623204329 37.0 33.0 40.0 22.0 41.0 62-63 33.183557318708324 37.0 32.5 39.5 20.0 41.0 64-65 32.7282903407806 36.0 32.0 39.0 18.5 40.5 66-67 32.207126035601625 36.0 31.0 39.0 14.0 40.0 68-69 31.700168345938337 35.0 31.0 38.5 11.5 40.0 70-71 31.200023013369453 35.0 30.5 38.0 8.0 40.0 72-73 30.686516221284684 35.0 30.0 37.0 6.5 39.0 74-75 30.097612377195407 34.0 29.0 36.5 2.0 39.0 76-77 29.525948002348162 34.0 29.0 36.0 2.0 39.0 78-79 28.9560972865924 34.0 28.0 35.5 2.0 37.5 80-81 28.356571601844728 34.0 27.0 35.0 2.0 37.0 82-83 27.75636094100126 33.0 26.0 35.0 2.0 36.5 84-85 27.15202985913305 33.0 25.5 35.0 2.0 36.0 86-87 26.52407523944183 32.0 24.5 35.0 2.0 35.5 88-89 25.89713537800173 32.0 23.5 34.5 2.0 35.0 90-91 25.252510056214295 31.5 20.0 34.0 2.0 35.0 92-93 24.483263626998394 31.0 17.0 34.0 2.0 35.0 94-95 23.6122849706137 31.0 4.5 34.0 2.0 35.0 96-97 22.608064330076814 30.0 2.0 34.0 2.0 35.0 98-99 21.30739066079778 29.5 2.0 34.0 2.0 35.0 100-101 18.114759050592866 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1514947055884761 1101 2 0.1279299848909865 1101 3 0.007915860511040762 1101 4 0.0565657282328047 1101 5 0.0735078076739839 1101 6 0.16695356621021773 1101 7 0.049236514050988944 1101 8 0.14592134921696243 1101 9 0.32942671837071913 1101 10-11 0.1746553311677843 1101 12-13 0.25435319786666355 1101 14-15 0.3905434136223178 1101 16-17 0.29412825064250825 1101 18-19 0.402009645829736 1101 20-21 0.3572819049642746 1101 22-23 0.24883556734182832 1101 24-25 0.28036162236886497 1101 26-27 0.2697594397224705 1101 28-29 0.3080407306043682 1101 30-31 0.31292379320839814 1101 32-33 0.1718150924870656 1101 34-35 0.28044420899399825 1101 36-37 0.29462780002368305 1101 38-39 0.11994543150565562 1101 40-41 0.13996470014790674 1101 42-43 -0.016031932984702735 1101 44-45 0.08878137892303783 1101 46-47 0.1024985397079945 1101 48-49 0.16685587430412596 1101 50-51 0.07282575929503565 1101 52-53 0.20840290437103448 1101 54-55 0.3431278889119511 1101 56-57 0.4627354482338717 1101 58-59 0.47981989088864196 1101 60-61 0.4706896073054807 1101 62-63 0.47454342284457596 1101 64-65 0.5345044984206808 1101 66-67 0.6398372074121994 1101 68-69 0.6966070535667797 1101 70-71 0.6820223706121915 1101 72-73 0.8602640552668035 1101 74-75 0.8913872742432325 1101 76-77 0.6759356054041064 1101 78-79 0.8838362636867032 1101 80-81 1.1197438746460868 1101 82-83 1.2363170069567495 1101 84-85 1.2345975230612183 1101 86-87 1.6904210762388878 1101 88-89 1.5408174057588653 1101 90-91 1.606817836916921 1101 92-93 1.4989837543259625 1101 94-95 1.9276299426926222 1101 96-97 2.078023330275297 1101 98-99 2.3074584671374545 1101 100-101 2.9936868909968517 1102 1 0.11863510851533832 1102 2 0.1430022343159152 1102 3 0.05895182007009936 1102 4 0.08101406546122547 1102 5 0.0752601534749644 1102 6 -0.022807100713023942 1102 7 0.07398965863701079 1102 8 0.0688815860890486 1102 9 0.2428513476354226 1102 10-11 0.21390921138323904 1102 12-13 0.15852834705326302 1102 14-15 0.2858954572251875 1102 16-17 0.3649806912658491 1102 18-19 0.3343702839326781 1102 20-21 0.40008613858429243 1102 22-23 0.367540984076939 1102 24-25 0.23037959058469681 1102 26-27 0.369280672757192 1102 28-29 0.3218952331249554 1102 30-31 0.41408573783189695 1102 32-33 0.5620447120530869 1102 34-35 0.5594400466538403 1102 36-37 0.6824919010109838 1102 38-39 0.5160188722885266 1102 40-41 0.6413649460100288 1102 42-43 0.6203351131888724 1102 44-45 0.7163134593044092 1102 46-47 0.8955757063753538 1102 48-49 0.9820950265127095 1102 50-51 0.8446300112983778 1102 52-53 0.6835593256954482 1102 54-55 0.8255056946666386 1102 56-57 0.8515397672473881 1102 58-59 0.8944923623366705 1102 60-61 0.8736972672211252 1102 62-63 0.9953669838603076 1102 64-65 0.929196076664546 1102 66-67 0.9737625628181661 1102 68-69 1.0182479015479586 1102 70-71 0.9010264570470206 1102 72-73 0.9981864195282029 1102 74-75 1.1138705528318198 1102 76-77 1.0543996016657715 1102 78-79 1.2670588720904945 1102 80-81 1.4037998702483243 1102 82-83 1.5642873516914975 1102 84-85 1.796060088388728 1102 86-87 1.6297266076622137 1102 88-89 1.7647222785848165 1102 90-91 1.8726731566058739 1102 92-93 2.2412879615570134 1102 94-95 2.3763345410733443 1102 96-97 2.6864576582254784 1102 98-99 3.0799028738927916 1102 100-101 3.3080758145109606 1103 1 0.1465034297558354 1103 2 0.041655003391422696 1103 3 0.11872046989767426 1103 4 0.15499159527082185 1103 5 0.04605918923691377 1103 6 0.17244990986202424 1103 7 0.19989124035092942 1103 8 0.14019980856210879 1103 9 0.26537235664167724 1103 10-11 0.275590135157735 1103 12-13 0.30808987223297635 1103 14-15 0.4006076804730796 1103 16-17 0.4289834271000217 1103 18-19 0.46396010012480104 1103 20-21 0.3596838585630522 1103 22-23 0.25595350673605566 1103 24-25 0.08320504484317581 1103 26-27 0.20454023024079504 1103 28-29 0.19329428706202378 1103 30-31 0.16235521510581918 1103 32-33 0.2860381450945013 1103 34-35 0.31305743402786845 1103 36-37 0.5184814527072419 1103 38-39 0.4500413855252816 1103 40-41 0.41291262939939344 1103 42-43 0.43277741913357204 1103 44-45 0.6819167418271164 1103 46-47 0.7718524489151832 1103 48-49 0.7788828128306662 1103 50-51 0.6654702400179389 1103 52-53 0.6385477988417065 1103 54-55 0.679302979877356 1103 56-57 0.6547803197710991 1103 58-59 0.7777737012692185 1103 60-61 0.9624597207669368 1103 62-63 1.198039922538399 1103 64-65 1.201810651677583 1103 66-67 1.2457120120641392 1103 68-69 1.2893301463668507 1103 70-71 1.2109140821803415 1103 72-73 1.0655222575209038 1103 74-75 1.2881645928266572 1103 76-77 1.4381296389247709 1103 78-79 1.4406689535437351 1103 80-81 1.5530666475600299 1103 82-83 1.6948220948722401 1103 84-85 2.086371791732475 1103 86-87 2.089934449749098 1103 88-89 2.131097465716831 1103 90-91 2.269696741218244 1103 92-93 2.3353247877024046 1103 94-95 2.786618790416888 1103 96-97 2.812155068748872 1103 98-99 2.8891738003935536 1103 100-101 3.472076696960965 1104 1 0.2725593196997522 1104 2 0.23221269712245984 1104 3 0.17718477974317537 1104 4 0.1932411935670686 1104 5 0.10273523098067727 1104 6 0.322384491242552 1104 7 0.43775447484802754 1104 8 0.4206188697332962 1104 9 0.30579789998628826 1104 10-11 0.27405121585422876 1104 12-13 0.3129834356615788 1104 14-15 0.4861832628539844 1104 16-17 0.6030214272002965 1104 18-19 0.6141429857544267 1104 20-21 0.5200924324610199 1104 22-23 0.4126672119368351 1104 24-25 0.38985915105531177 1104 26-27 0.4691154210649984 1104 28-29 0.49450405401415765 1104 30-31 0.5393808943015017 1104 32-33 0.4005783286008935 1104 34-35 0.585855315562867 1104 36-37 0.5031679148730817 1104 38-39 0.481062405434983 1104 40-41 0.5120525774942664 1104 42-43 0.6681465687631487 1104 44-45 0.6381246166531227 1104 46-47 0.6567400017096787 1104 48-49 0.6560193634548952 1104 50-51 0.5054499399518022 1104 52-53 0.5593217321481418 1104 54-55 0.6196983208301248 1104 56-57 0.7283950685659875 1104 58-59 0.7564856408835325 1104 60-61 0.8134492669192994 1104 62-63 0.961823195904671 1104 64-65 0.9407952842708553 1104 66-67 1.1947060814126544 1104 68-69 1.1998013932123115 1104 70-71 1.3550215716572573 1104 72-73 1.3208652755157964 1104 74-75 1.4225929916701325 1104 76-77 1.4116968756566095 1104 78-79 1.534979503370522 1104 80-81 1.818768906889943 1104 82-83 1.8925103285933922 1104 84-85 2.179562286454818 1104 86-87 2.12387662589709 1104 88-89 2.307286341686467 1104 90-91 2.3975083690197216 1104 92-93 2.4677164649737335 1104 94-95 2.716859621663115 1104 96-97 2.7804373345728983 1104 98-99 2.746957094053915 1104 100-101 3.1134201602322307 1105 1 0.2547967987727908 1105 2 0.25402949829848254 1105 3 0.1157990198451273 1105 4 0.13167642981603933 1105 5 0.13185350510717342 1105 6 0.19637520000045328 1105 7 0.266657253458888 1105 8 0.2609480609593433 1105 9 0.34088202708655757 1105 10-11 0.36306166685287167 1105 12-13 0.4081242975619972 1105 14-15 0.3901949590285412 1105 16-17 0.39063586797498573 1105 18-19 0.6148866368489081 1105 20-21 0.49940100024491585 1105 22-23 0.41041882933085816 1105 24-25 0.3002647392415625 1105 26-27 0.4234049029556175 1105 28-29 0.4230573354531231 1105 30-31 0.3566452820502235 1105 32-33 0.5003696791975258 1105 34-35 0.6325494127380935 1105 36-37 0.6112882833446562 1105 38-39 0.6442990023644697 1105 40-41 0.6145694972838882 1105 42-43 0.8099755610436858 1105 44-45 0.8574446909303006 1105 46-47 0.9263229447151673 1105 48-49 0.7839048977138745 1105 50-51 1.035062672966923 1105 52-53 0.9531548609878939 1105 54-55 0.9370016497679146 1105 56-57 1.0586411611788122 1105 58-59 1.0957588663979223 1105 60-61 1.3465970119889263 1105 62-63 1.4163673657334783 1105 64-65 1.4749033693096223 1105 66-67 1.5200991283377334 1105 68-69 1.4349215409124767 1105 70-71 1.5256960927198797 1105 72-73 1.577495562528064 1105 74-75 1.6841301825469621 1105 76-77 1.7376811063592896 1105 78-79 1.8557471849471625 1105 80-81 2.2098191538995557 1105 82-83 2.1985616951621907 1105 84-85 2.391949572910285 1105 86-87 2.4273462544389695 1105 88-89 2.464756345194317 1105 90-91 2.364439134411935 1105 92-93 2.5412674209328188 1105 94-95 2.5552103492509666 1105 96-97 2.416105296871546 1105 98-99 2.3464959550052953 1105 100-101 3.052215445459165 1106 1 -0.018258719461524464 1106 2 -0.01876317861772847 1106 3 0.005145933428359228 1106 4 -0.01799115017527697 1106 5 -0.03118651550565943 1106 6 -0.047247616836450845 1106 7 -0.07696511437182352 1106 8 0.02971919663070821 1106 9 -0.05461644404734756 1106 10-11 -0.32176033500326184 1106 12-13 -0.2434023407666217 1106 14-15 -0.4563780702746314 1106 16-17 -0.1726762503913335 1106 18-19 0.018115267694319925 1106 20-21 0.06221955718736183 1106 22-23 -0.12481157819752298 1106 24-25 -0.2971134790535004 1106 26-27 -0.23042548253692274 1106 28-29 0.028078848030652637 1106 30-31 0.10904081644186192 1106 32-33 0.17960103585593146 1106 34-35 0.13859320880224857 1106 36-37 -0.08623489487315084 1106 38-39 -0.1627811690729999 1106 40-41 0.19528526841921945 1106 42-43 0.3996724922244965 1106 44-45 0.2027245823951418 1106 46-47 0.37851259249743663 1106 48-49 0.5790000576688712 1106 50-51 0.6365195944492115 1106 52-53 0.7541307932197299 1106 54-55 0.7533940369715211 1106 56-57 0.4345743601096288 1106 58-59 0.27042538431690133 1106 60-61 0.5582143219375553 1106 62-63 0.5095599828282431 1106 64-65 0.7818645541057165 1106 66-67 0.9977697049273075 1106 68-69 0.6880218423337894 1106 70-71 0.6372057447987984 1106 72-73 0.4361941767913322 1106 74-75 0.7358399808637408 1106 76-77 1.057513571786675 1106 78-79 1.087689451039978 1106 80-81 1.1192791272424039 1106 82-83 1.1631786255278946 1106 84-85 1.2427708915862148 1106 86-87 1.1506802083218908 1106 88-89 1.553458209571648 1106 90-91 1.6153976762884987 1106 92-93 1.6635267993769958 1106 94-95 1.8033297904247654 1106 96-97 2.1255478625896487 1106 98-99 2.141563829199285 1106 100-101 2.492803463970656 1107 1 0.04571564334763423 1107 2 0.15200221365006072 1107 3 0.10276687445573884 1107 4 -0.04272962284258597 1107 5 0.07804315512134963 1107 6 0.2060155655954361 1107 7 0.16545328328999886 1107 8 0.07741658371247695 1107 9 0.19445191801298733 1107 10-11 0.17465269991713228 1107 12-13 0.12539065046932762 1107 14-15 0.3463809670173603 1107 16-17 0.27378744486687623 1107 18-19 0.4547764368462097 1107 20-21 0.39411198467877995 1107 22-23 0.23684495812062067 1107 24-25 0.30423418563729143 1107 26-27 0.3715625274486811 1107 28-29 0.34905016503096675 1107 30-31 0.3160882590660563 1107 32-33 0.361510155301211 1107 34-35 0.5241548234281765 1107 36-37 0.4469734980681679 1107 38-39 0.5427053781748512 1107 40-41 0.6338270758329188 1107 42-43 0.6175848324776894 1107 44-45 0.6129342477756197 1107 46-47 0.6167399516928143 1107 48-49 0.5694237582492647 1107 50-51 0.45521643934331735 1107 52-53 0.4337504848431877 1107 54-55 0.7221075394385252 1107 56-57 0.707998190993834 1107 58-59 0.624319044709388 1107 60-61 0.823174421119738 1107 62-63 1.005958233937804 1107 64-65 0.8824929692089967 1107 66-67 1.181950374097596 1107 68-69 1.2216550119793936 1107 70-71 1.109813578086925 1107 72-73 1.1672675467816376 1107 74-75 1.391113314616522 1107 76-77 1.3912116870570799 1107 78-79 1.4975589168665095 1107 80-81 1.450905961853163 1107 82-83 1.7094890848677728 1107 84-85 1.7863850244967985 1107 86-87 1.9799973303471212 1107 88-89 1.810095882778608 1107 90-91 1.968419779460934 1107 92-93 2.449863257595368 1107 94-95 2.6966759409782846 1107 96-97 2.7303037113918336 1107 98-99 2.8626840844985075 1107 100-101 3.395447600109616 1108 1 -0.05867976212732273 1108 2 -0.03785217955384468 1108 3 0.09486928029631869 1108 4 -0.11336309052047255 1108 5 0.007682681839732197 1108 6 0.008577994717185788 1108 7 0.07498091818331432 1108 8 0.03505255008817443 1108 9 0.07410542060937075 1108 10-11 0.08508623915412272 1108 12-13 0.12860249314236682 1108 14-15 0.055085998366045885 1108 16-17 0.07785672477810834 1108 18-19 0.20670117780323238 1108 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-1.261286725243167 2316 68-69 -1.145443226601003 2316 70-71 -1.0701126448149765 2316 72-73 -1.3890260667806729 2316 74-75 -1.6152367856128684 2316 76-77 -1.5704908693090367 2316 78-79 -1.7262050928602193 2316 80-81 -1.7044148066410507 2316 82-83 -1.9338232726239433 2316 84-85 -1.8708347637470837 2316 86-87 -2.200968901647755 2316 88-89 -2.2691221901994147 2316 90-91 -2.041045353799383 2316 92-93 -2.239407547752954 2316 94-95 -2.093983093099709 2316 96-97 -1.9997964491129636 2316 98-99 -2.5607597767096166 2316 100-101 -2.7863655874605655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6431.0 3 1249.0 4 1218.0 5 1401.0 6 1638.0 7 1963.0 8 2491.0 9 3110.0 10 3890.0 11 5143.0 12 6783.0 13 8832.0 14 10175.0 15 12693.0 16 16071.0 17 20228.0 18 17658.0 19 13464.0 20 13643.0 21 14365.0 22 16334.0 23 18303.0 24 20829.0 25 23643.0 26 27360.0 27 32133.0 28 38202.0 29 44998.0 30 54585.0 31 67426.0 32 84191.0 33 107283.0 34 140433.0 35 189006.0 36 251884.0 37 293663.0 38 167172.0 39 11264.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95108786872136 18.88522456536521 14.505851938033787 22.657835627879642 2 15.072605501424855 19.83111297898131 41.166955722124285 23.92932579746956 3 19.308659817662736 24.466197978280313 29.62871771850525 26.5964244855517 4 12.359040395394016 16.252173619290115 37.33557933502853 34.05320665028734 5 13.46366136926315 37.84677609595332 33.87206301001537 14.817499524768166 6 32.0517860238846 35.99204189847755 17.37621827674029 14.579953800897547 7 28.95407156171795 33.06076303662992 20.060235518129588 17.92492988352254 8 26.624656976172002 35.114253541241816 19.293903044701242 18.967186437884937 9 26.642051134162664 17.0913160727068 20.387520119564428 35.87911267356611 10-11 25.281586318948918 26.888721870335687 26.780404584802337 21.049287225913066 12-13 26.071395873150298 24.157749573696197 27.717329106535892 22.05352544661761 14-15 22.65775335894107 25.49610934461096 26.36773745757872 25.478399838869247 16-17 21.926179152512066 29.166658322903626 26.773438226354372 22.133724298229932 18-19 21.788411693175014 27.27845879804349 29.61101796804581 21.322111540735687 20-21 22.6657818104844 26.22605024228659 29.70161848586614 21.40654946136287 22-23 22.31277146774742 26.20974882287093 29.659304132846586 21.818175576535058 24-25 22.06291642791776 26.457803449026546 29.791000422524778 21.688279700530916 26-27 21.85994111191597 26.820398209783935 29.640097223700334 21.67956345459976 28-29 21.54769632802346 27.027323176678696 29.820007432814993 21.604973062482852 30-31 21.722827475494466 26.546542989576366 29.807758056620575 21.922871478308586 32-33 21.506578266001927 26.676209009932773 29.726363817906226 22.090848906159074 34-35 22.158230980725378 26.71599046711699 29.438898854737978 21.686879697419656 36-37 22.14373496002499 26.416947298440018 29.438015564335906 22.001302177199083 38-39 21.75026137499696 26.8116255021904 29.59331337682194 21.844799745990702 40-41 21.976815626798405 26.807989821941174 29.43885670743916 21.776337843821256 42-43 21.930582866152868 26.692648365435723 29.203608679458803 22.17316008895261 44-45 21.948212366029722 26.885395873489376 29.051805369853234 22.11458639062767 46-47 22.178086337990536 27.164242896188778 28.574620413912903 22.083050351907787 48-49 22.284220347115113 27.198351370581992 28.365043357202758 22.152384925100144 50-51 22.117644164281167 27.422182500424842 28.2761852503074 22.18398808498659 52-53 22.49045726994231 27.40872926972257 28.09429513408748 22.006518326247637 54-55 22.077289351300877 27.224696523060587 28.280314508303277 22.417699617335263 56-57 22.281863492392187 27.610707856420863 28.092106053472925 22.015322597714025 58-59 22.477495937014645 27.16024562937738 28.110110549234403 22.25214788437357 60-61 22.290079305264488 27.173415303367594 28.64099315921661 21.895512232151308 62-63 22.177183056134155 27.32078102928588 28.46957053456593 22.03246538001403 64-65 22.031415265789576 27.273671137918154 28.393152458253983 22.30176113803828 66-67 22.03510734936423 27.536147321357067 28.08816765858715 22.34057767069155 68-69 22.03838390714322 27.249279168403024 28.35598913025136 22.3563477942024 70-71 22.04052867064942 27.36247301029878 28.317670320781662 22.279327998270144 72-73 22.230946623256337 27.2451316412 28.211881089560407 22.31204064598326 74-75 21.896490172161666 27.672991237444876 28.061201721845514 22.36931686854794 76-77 22.103306804067323 27.421803887106734 28.030283526166826 22.444605782659117 78-79 22.1189770258461 27.52118562252477 28.000921082930358 22.35891626869877 80-81 21.973314797572616 27.771775585714302 27.97157850023384 22.283331116479236 82-83 22.202200759213877 27.693687633142726 27.875911548608677 22.228200059034712 84-85 22.126610357770847 27.598995317506898 27.770885028949543 22.503509295772712 86-87 22.187604753356972 27.39841570021863 27.98277497291429 22.431204573510115 88-89 22.178187744958663 27.480421899554862 27.71188656286417 22.62950379262231 90-91 22.12106563847923 27.526314886216717 27.883367847785546 22.469251627518506 92-93 22.406823077970355 27.342708706351832 27.87482138015223 22.375646835525586 94-95 22.126325496147572 27.168344946768496 27.952816713278672 22.752512843805256 96-97 22.209509207718583 27.104903346128985 27.96975383154366 22.71583361460878 98-99 22.07840872457746 27.325489428806883 27.572345588325735 23.023756258289918 100-101 22.475217056316527 26.40330862522515 28.423094940796062 22.698379377662263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2186.0 1 1276.5 2 378.5 3 384.0 4 331.0 5 320.0 6 526.0 7 1140.0 8 1944.0 9 2293.0 10 2077.0 11 1833.0 12 1948.5 13 2190.0 14 2345.0 15 2484.5 16 2600.5 17 2711.5 18 2793.0 19 2941.5 20 3243.5 21 3749.5 22 4536.0 23 5561.0 24 6744.5 25 8266.5 26 10475.5 27 13004.0 28 15031.0 29 17381.5 30 20618.5 31 23943.0 32 27647.5 33 31344.0 34 35125.5 35 38505.5 36 42536.0 37 46935.5 38 49519.0 39 50714.5 40 51617.0 41 54617.5 42 57711.5 43 60652.5 44 64111.0 45 66361.5 46 66182.0 47 67359.0 48 72204.0 49 71597.0 50 66442.5 51 62365.0 52 59024.0 53 57434.0 54 55489.0 55 50694.0 56 45489.5 57 41124.0 58 37063.5 59 32794.0 60 27448.5 61 22076.0 62 17634.5 63 13459.0 64 10015.0 65 7849.0 66 5990.0 67 4576.0 68 3338.0 69 2306.5 70 1716.5 71 1332.5 72 1103.5 73 943.0 74 739.0 75 563.0 76 417.5 77 265.0 78 171.5 79 122.0 80 82.0 81 50.5 82 33.0 83 26.5 84 14.5 85 10.5 86 9.5 87 5.5 88 5.0 89 6.0 90 4.0 91 2.0 92 3.5 93 3.5 94 1.5 95 2.5 96 2.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31379271749632814 2 0.2662812450746821 3 0.2696504480468902 4 0.265253352642483 5 0.26496782696687216 6 0.20003928833296405 7 0.19130220265927192 8 0.1978121880631994 9 0.19866876509003198 10-11 0.20058178711662467 12-13 0.2042079631968825 14-15 0.20026770887345274 16-17 0.1909310192809778 18-19 0.18544892630924945 20-21 0.18422116590412277 22-23 0.19218733225366558 24-25 0.19013154738926744 26-27 0.19609903400953427 28-29 0.19989652549515866 30-31 0.20218073090004546 32-33 0.20026770887345274 34-35 0.2010671807651631 36-37 0.1892464177948738 38-39 0.18207972333704137 40-41 0.17599802644653018 42-43 0.1861056353631544 44-45 0.18770457914657518 46-47 0.19398614401001393 48-49 0.20115283846784637 50-51 0.19672719049587817 52-53 0.20680624684494128 54-55 0.19484272103684652 56-57 0.1724004029338334 58-59 0.15701056901840843 60-61 0.1734568479335936 62-63 0.16700396766478828 64-65 0.17274303374456643 66-67 0.17314276969042164 68-69 0.17140106306919545 70-71 0.17334263766334923 72-73 0.18187985536411377 74-75 0.18639116103876524 76-77 0.1870193175251091 78-79 0.18350735171509563 80-81 0.1817941976614305 82-83 0.17314276969042164 84-85 0.16800330752942627 86-87 0.17142961563675652 88-89 0.18028091158069298 90-91 0.17679749833824057 92-93 0.1730285594201773 94-95 0.1734282953660325 96-97 0.18079485779679252 98-99 0.18128025144533097 100-101 0.16429147374648517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1751156.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.27035170874706 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33463608680098 40.66372911903104 2 13.179388847667859 14.041413584432108 3 4.347332221286339 6.947517492680758 4 1.9353376125631385 4.123844611856209 5 1.0967472747317415 2.9212056530284856 6 0.6603751206576319 2.1107048962282993 7 0.43739571933700505 1.6310156663487871 8 0.2980591801261023 1.2702173884270607 9 0.23183139157187702 1.1114765789545933 >10 1.3056123055776672 12.955147875980966 >50 0.10962200469618259 4.0549616048663655 >100 0.05991148674475559 5.69062905518064 >500 0.0030005985910048355 1.0606720955553355 >1k 5.358211769651493E-4 0.6373737323398988 >5k 2.143284707860597E-4 0.7800906450895403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7668 0.43788217611680513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5997 0.34245949532765785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4015 0.22927711751551547 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2330 0.13305496483465779 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2161 0.12340419699901095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 2.8552567561085363E-5 0.0 0.0 2.8552567561085363E-5 0.0 20-21 5.7105135122170725E-5 0.0 0.0 8.565770268325609E-5 0.0 22-23 5.7105135122170725E-5 0.0 0.0 1.7131540536651218E-4 0.0 24-25 5.7105135122170725E-5 0.0 0.0 2.284205404886829E-4 0.0 26-27 5.7105135122170725E-5 0.0 0.0 3.997359458551951E-4 0.0 28-29 5.7105135122170725E-5 0.0 0.0 0.0011421027024434145 0.0 30-31 5.7105135122170725E-5 0.0 0.0 0.0030551247290361338 0.0 32-33 5.7105135122170725E-5 0.0 0.0 0.006310117430999865 0.0 34-35 5.7105135122170725E-5 0.0 0.0 0.009764978105891194 0.0 36-37 5.7105135122170725E-5 0.0 0.0 0.013591022159076632 0.0 38-39 5.7105135122170725E-5 0.0 0.0 0.017873907293239438 0.0 40-41 5.7105135122170725E-5 0.0 0.0 0.022470870670574183 0.0 42-43 5.7105135122170725E-5 0.0 0.0 0.02795296364230257 0.0 44-45 5.7105135122170725E-5 0.0 0.0 0.03363492458695856 0.0 46-47 1.1421027024434145E-4 0.0 0.0 0.041743853774306804 0.0 48-49 1.1421027024434145E-4 0.0 0.0 0.05336474877166854 0.0 50-51 1.1421027024434145E-4 0.0 0.0 0.06407196160707555 0.0 52-53 1.1421027024434145E-4 0.0 0.0 0.0834877075486136 0.0 54-55 1.1421027024434145E-4 0.0 0.0 0.1135535611904365 0.0 56-57 1.1421027024434145E-4 0.0 0.0 0.15983727320695584 0.0 58-59 1.1421027024434145E-4 0.0 0.0 0.2029230976566337 0.0 60-61 1.1421027024434145E-4 0.0 0.0 0.24480971426874593 0.0 62-63 1.1421027024434145E-4 0.0 0.0 0.2959473627706498 0.0 64-65 1.1421027024434145E-4 0.0 0.0 0.3523101311362323 0.0 66-67 2.284205404886829E-4 0.0 0.0 0.43551231300923504 0.0 68-69 2.284205404886829E-4 0.0 0.0 0.5349323532569343 0.0 70-71 2.284205404886829E-4 0.0 0.0 0.6391206722873348 0.0 72-73 2.284205404886829E-4 0.0 0.0 0.7483913483436084 0.0 74-75 2.284205404886829E-4 0.0 0.0 0.8675697653435788 0.0 76-77 2.284205404886829E-4 0.0 0.0 1.007848529771191 0.0 78-79 2.5697310804976826E-4 0.0 0.0 1.1494407123066135 0.0 80-81 2.855256756108536E-4 0.0 0.0 1.304195628487696 0.0 82-83 2.855256756108536E-4 0.0 0.0 1.4651178992619731 0.0 84-85 2.855256756108536E-4 0.0 0.0 1.6549068158405076 0.0 86-87 2.855256756108536E-4 0.0 0.0 1.8612562216044717 0.0 88-89 2.855256756108536E-4 0.0 0.0 2.0996987133070952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6345 0.0 34.477608 1 GTATCAA 9590 0.0 33.37393 1 ATCAACG 11545 0.0 27.588625 3 TCAACGC 11680 0.0 27.269749 4 CAACGCA 11705 0.0 27.168556 5 CTATGCG 105 6.903015E-5 27.145655 9 TATCAAC 11695 0.0 27.112827 2 AACGCAG 12085 0.0 26.494246 6 GTACATG 8765 0.0 25.22969 1 TACATGG 9075 0.0 24.096857 2 ACATGGG 9085 0.0 23.233103 3 ACGCAGA 13605 0.0 23.114546 7 CGCAGAG 13645 0.0 23.0816 8 GAGTACT 6750 0.0 22.906277 12-13 CCTATAC 460 0.0 21.702545 3 GTATACG 110 0.0028581354 21.616642 1 GCAGAGT 14365 0.0 21.462358 9 GTACTTT 7220 0.0 21.18427 14-15 AGTACTT 7075 0.0 21.081936 12-13 CATGGGG 3525 0.0 20.903633 4 >>END_MODULE