FastQCFastQC Report
Thu 26 May 2016
SRR937600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751156
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85050.485679174214062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73800.42143589720162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50380.2876956707454961No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24180.13808021672540882No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.10684370781358142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68450.031.9421271
GTATCAA101400.031.8281381
GTACATG83700.026.8037761
ATCAACG126850.025.3537353
TACATGG87550.025.1228312
TCAACGC128200.025.0867484
TATCAAC129600.024.7790952
CAACGCA129800.024.7775145
AACGCAG131850.024.3880946
ACATGGG88750.023.9267043
CGTTCGA1252.2465238E-422.815011
AGTACTT81700.022.32560712-13
TAGCGCG2354.647518E-922.2366094
GAGTACT77500.021.75809112-13
ACGCAGA147800.021.725337
CGCAGAG148700.021.5618958
GTGTAGC9350.021.3509461
ATGGGAG21800.021.137765
AGAGTAC126100.021.03789110-11
CATGGGA52150.020.9516054
Produced by FastQC (version 0.11.5)