FastQCFastQC Report
Thu 26 May 2016
SRR937599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305324
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28890.9462079626888158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.6953269313909158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19700.6452162293170534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.5037271881673239No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.21616381286764225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.15557244107898494No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4180.13690374814950676No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4160.13624870629233207No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.13002580864917268No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3250.10644430179088443No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10513421807653509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATC205.4039474E-447.4682788-89
GTATCAA24550.034.0777661
GGTATCA15700.033.001741
ACAGCGT300.003962447431.64551268-69
GTATAAC953.46522E-530.0218621
GTGTAGC1802.0736479E-1029.0489311
GACGTGG1153.8354083E-628.9293587
GAACGCA1153.8452436E-628.919859
ATCAACG29500.027.8762323
TATCAAC30300.027.6108672
TCAACGC29850.027.5584414
CAACGCA30000.027.4206475
AACGCAG31750.026.0419036
CGTGGAA1109.43908E-525.9151869
GGAACGC1309.971289E-625.5829418
TGTACTG2101.2405508E-924.9072765
GGGAACG1351.3326711E-524.6435267
GTACTGC1000.001647025123.7594936
GCTGATC1000.001648602323.7555878
CCCTGGC3850.023.4547847
Produced by FastQC (version 0.11.5)