FastQCFastQC Report
Thu 26 May 2016
SRR937599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305324
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45431.4879275785722708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37121.2157576869161941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.9390025022598943No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.5715240203849026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12230.40055809566231276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.3081971938006839No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.26725707772726676No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.257758970798234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.13428358072080807No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.12445795286318796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.039.9619181
CAGGACG500.001611538638.0115974
GTATCAA27150.033.081811
TATCAAC32950.027.2541452
ATCAACG32500.027.1929133
GTACTAG700.00836292927.155591
CGCTAAT350.00838946127.14224814-15
ATCGGTG350.00838946127.14224880-81
TCAACGC33450.026.4206184
CAACGCA34200.025.8412195
GGACTGC950.001224651724.9994416
AACGCAG35550.024.9853766
GAGTACT25850.023.2441612-13
GTACATG21500.022.9875241
GTACTAC1050.002186584822.629661
CGCAGAG39350.022.4518478
TACATGG21600.022.43742
ACGCAGA39500.022.3665877
GGAGAGT2354.616595E-922.2335438
ACATGGG22150.021.451243
Produced by FastQC (version 0.11.5)