FastQCFastQC Report
Thu 26 May 2016
SRR937598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311859
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29140.9343966343764329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.660554930272976No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19020.609891008436505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.5037532987664297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.22029186266870607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.1561603160402618No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4530.14525795311342626No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4180.13403493245344852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.11575744166434189No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3570.11447481073177301No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.10164850140608415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC1651.8189894E-1234.585171
GAGAGTA1800.034.3173949
GGTATCA17050.032.6327821
GTGTAGG1252.1238702E-730.4349481
ATAGCCC1751.5097612E-1029.8674478
GTATCAA25700.029.2359091
GTATATG2500.028.5327641
GTACATG26550.028.1208041
TACATGG27000.027.2867012
GGAGAGT2305.456968E-1226.8570948
AAGGCGG458.958476E-426.38103594-95
ACATGGG28100.025.7110823
ATCAACG30500.024.7788073
CATGGGC5000.024.7164694
TCAACGC30950.024.572114
ATGGGAG5050.024.4638865
GAGTACT17350.024.37048512-13
CAACGCA31200.024.367385
TATCAAC31050.024.186812
AACGCAG32650.023.4307486
Produced by FastQC (version 0.11.5)