FastQCFastQC Report
Thu 26 May 2016
SRR937598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311859
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45121.4468076919377026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37401.1992599219519078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.9212496673176019No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.5627543216645985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11520.369397708579839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.29436379902455917No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.2632599989097637No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.24049329985666598No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.12409454272603966No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.11383349526548857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG200.00207051971.2685242
GGTATCA18650.041.7871
GTATCAA29250.034.7667471
GTATAGC1850.033.392411
TCCGCGC300.003944555831.67489894-95
ATAGGGG600.00394155731.6748983
TACTAGG1004.9329446E-528.5074082
TATCAAC36050.028.0724832
GTATATA2900.027.8565351
ATCAACG36350.027.8407973
GTAGGAC2400.027.7155363
TCAACGC37000.027.3517044
CAACGCA37100.027.277985
GTACTAG1056.870277E-527.154271
CACGACC350.00837804727.14991494-95
TAGCCCT2800.027.1499144
ACGACCC350.00837804727.14991494-95
CCTCGGA350.00839116527.14120930-31
TGTAGGA3250.026.3145312
AACGCAG38750.026.2306636