FastQCFastQC Report
Thu 26 May 2016
SRR937597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279532
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27400.9802097792023812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.7229941473605884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17650.6314125037562784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.5648727158250219No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.25184951991185267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.1631298026701773No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.16169883948885994No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3760.1345105390438304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3740.13379505745317172No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3350.11984316643532762No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.11411931371005823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCG156.610941E-495.0060969
GGTATCA16900.033.1856581
GTAGCCC1151.0310214E-733.04563
GTATCAA24850.032.323281
TAGCCCT1901.2732926E-1130.0073034
CTATTAG856.372439E-427.9580061
TATCAAC29250.027.6135582
ATCAACG29550.027.4890733
TAGGACG2253.6379788E-1227.4511284
TCAACGC29900.027.1721654
CAACGCA30000.026.923225
AACGCAG31500.025.7873696
GTCTTAA1501.0497715E-625.3485931
GGTGACC950.001223768525.0016048
AGGACGT2301.3278623E-1024.7886435
TATAGCC1151.2762424E-424.7886432
GACGTGG1151.2777977E-424.78427
GGACGTG2151.6461854E-924.3038856
GTAGGAC2551.8189894E-1124.217243
GAGTACT17550.024.08554612-13
Produced by FastQC (version 0.11.5)