##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937597_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279532 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782879956498718 33.0 31.0 34.0 30.0 34.0 2 31.870211639454517 33.0 31.0 34.0 30.0 34.0 3 31.881484051915344 33.0 31.0 34.0 30.0 34.0 4 35.47706166020348 37.0 35.0 37.0 33.0 37.0 5 35.50247556630368 37.0 35.0 37.0 33.0 37.0 6 35.57062518781392 37.0 35.0 37.0 33.0 37.0 7 35.537823934290174 37.0 35.0 37.0 33.0 37.0 8 35.51605540689438 37.0 35.0 37.0 33.0 37.0 9 37.14044188143039 39.0 37.0 39.0 34.0 39.0 10-11 37.03243635791251 39.0 37.0 39.0 33.0 39.0 12-13 36.920289984688694 39.0 37.0 39.0 33.0 39.0 14-15 38.0192482434927 40.0 38.0 41.0 33.0 41.0 16-17 37.97990033341442 40.0 38.0 41.0 33.0 41.0 18-19 37.986801153356325 40.0 38.0 41.0 33.0 41.0 20-21 37.951145128285845 40.0 38.0 41.0 33.0 41.0 22-23 37.75793826824835 40.0 37.5 41.0 33.0 41.0 24-25 37.5951823047093 40.0 37.0 41.0 32.0 41.0 26-27 37.52805045576177 40.0 37.0 41.0 32.5 41.0 28-29 37.514540374626165 40.0 37.0 41.0 33.0 41.0 30-31 37.29676387676545 39.5 36.5 41.0 32.0 41.0 32-33 37.01459940185739 39.0 35.5 41.0 32.0 41.0 34-35 36.973362620379774 39.0 35.0 41.0 31.5 41.0 36-37 36.86759834294463 39.0 35.0 41.0 31.0 41.0 38-39 36.714996851881 39.0 35.0 41.0 31.0 41.0 40-41 36.44950488673926 39.0 35.0 41.0 30.5 41.0 42-43 36.18007956155289 39.0 35.0 41.0 30.0 41.0 44-45 35.855089936035945 39.0 35.0 41.0 29.5 41.0 46-47 35.43564064221628 39.0 35.0 41.0 27.5 41.0 48-49 35.015624329236005 39.0 35.0 40.0 24.5 41.0 50-51 34.07811449136413 38.0 34.0 39.5 23.5 40.5 52-53 34.14037391067927 38.0 34.5 39.5 23.0 40.5 54-55 34.46777292045276 38.0 35.0 40.0 23.0 41.0 56-57 34.30072048996179 38.0 34.5 40.0 21.5 41.0 58-59 34.08397786299959 38.0 34.0 40.0 19.5 41.0 60-61 33.78502282386274 38.0 34.0 40.0 17.5 41.0 62-63 33.439715309875076 38.0 34.0 40.0 12.5 41.0 64-65 33.0839313566962 37.5 33.0 40.0 8.0 41.0 66-67 32.69523882775496 37.0 33.0 40.0 4.5 41.0 68-69 32.24889636964641 36.0 32.0 39.0 2.0 41.0 70-71 31.812064450581687 36.0 31.5 39.0 2.0 40.5 72-73 31.357576234563485 35.0 31.0 39.0 2.0 40.0 74-75 30.900032912153172 35.0 31.0 38.0 2.0 40.0 76-77 30.383440894065796 35.0 30.0 37.0 2.0 39.0 78-79 29.853938010674987 35.0 29.5 37.0 2.0 39.0 80-81 29.30897893622197 34.0 29.0 36.0 2.0 38.5 82-83 28.766046463374497 34.0 29.0 36.0 2.0 37.5 84-85 28.275955883405118 34.0 28.0 35.0 2.0 37.0 86-87 27.778120215216862 34.0 26.5 35.0 2.0 36.5 88-89 27.278179242448093 34.0 26.0 35.0 2.0 36.0 90-91 26.760900362033688 33.0 25.5 35.0 2.0 36.0 92-93 26.224255183664123 33.0 24.5 35.0 2.0 35.0 94-95 25.619912925890418 32.5 21.5 35.0 2.0 35.0 96-97 24.91482370533606 32.0 15.5 35.0 2.0 35.0 98-99 24.041285076484982 32.0 2.0 34.0 2.0 35.0 100-101 21.92010038206717 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12497457322714212 1101 2 -0.014668716461741838 1101 3 0.09205452875896825 1101 4 -0.09218878131257924 1101 5 0.018482956520202265 1101 6 -7.646720462233247E-4 1101 7 0.06553201144920706 1101 8 0.09914282990940393 1101 9 0.14547959971803692 1101 10-11 0.06759274972856133 1101 12-13 0.20933553285194506 1101 14-15 0.5850861306507653 1101 16-17 0.5207403988394432 1101 18-19 0.38196322750518874 1101 20-21 0.27253862965436326 1101 22-23 0.2932916679377229 1101 24-25 0.367570166393584 1101 26-27 0.5108352335127293 1101 28-29 -0.11699756669720784 1101 30-31 0.3722766231510306 1101 32-33 0.2539540584017388 1101 34-35 0.27886742157775046 1101 36-37 0.31526809383397847 1101 38-39 0.5066069181462183 1101 40-41 0.4677275579841549 1101 42-43 0.32095225099708813 1101 44-45 0.1666257241900837 1101 46-47 0.6840245996868362 1101 48-49 0.9773894616779728 1101 50-51 0.804667912402067 1101 52-53 0.932973530928237 1101 54-55 1.2813637748596918 1101 56-57 1.28669945737672 1101 58-59 1.5823661954354975 1101 60-61 1.6360567585147834 1101 62-63 1.4084609204731748 1101 64-65 1.4185211495424 1101 66-67 1.5758132850102058 1101 68-69 1.5358979383590778 1101 70-71 1.4184503153627865 1101 72-73 1.495143539563216 1101 74-75 1.37857336280722 1101 76-77 1.3066243579654149 1101 78-79 1.4915124126804464 1101 80-81 1.397113184479121 1101 82-83 1.377232939588744 1101 84-85 1.5987889933203334 1101 86-87 1.2781933462987034 1101 88-89 1.426855929111671 1101 90-91 1.7698853742228913 1101 92-93 1.92158049087422 1101 94-95 1.6865460706720228 1101 96-97 1.8841903663168509 1101 98-99 2.117617457198026 1101 100-101 2.458873282229437 1102 1 0.12322790016946428 1102 2 0.053754950753987174 1102 3 -0.16575654139032636 1102 4 0.1278546207843121 1102 5 -0.010212104530339161 1102 6 -0.1848967608375034 1102 7 0.08869722702166882 1102 8 0.22885234961029255 1102 9 0.1855873205850287 1102 10-11 -0.04191257095018841 1102 12-13 -0.017178965017173198 1102 14-15 0.23905899126728514 1102 16-17 0.17819065637598186 1102 18-19 0.2355661244644267 1102 20-21 0.5388013430697995 1102 22-23 0.4409829901329303 1102 24-25 0.6483503583040644 1102 26-27 0.7270155875274256 1102 28-29 0.6240409461273231 1102 30-31 0.5928037233157539 1102 32-33 0.6050273451954737 1102 34-35 0.4501488292698568 1102 36-37 0.4051444240036659 1102 38-39 0.4164691295614915 1102 40-41 0.33903772610191396 1102 42-43 0.4748139917872152 1102 44-45 0.5599180085522519 1102 46-47 0.266142151390234 1102 48-49 0.3324630622218052 1102 50-51 0.4366390473930508 1102 52-53 0.2818636399563914 1102 54-55 0.3888558657426344 1102 56-57 0.17214798928772268 1102 58-59 0.12008810826525718 1102 60-61 0.43524362284401263 1102 62-63 0.48484599642684856 1102 64-65 0.6891436820286074 1102 66-67 0.3216495597507034 1102 68-69 0.559376904029584 1102 70-71 0.8379799516741322 1102 72-73 0.998437915592703 1102 74-75 0.9587495857789499 1102 76-77 0.9362292943002899 1102 78-79 0.9002045976884254 1102 80-81 0.836284884220273 1102 82-83 0.5433897623460844 1102 84-85 1.0340714991377844 1102 86-87 0.7733106103978464 1102 88-89 1.1349899004035358 1102 90-91 1.0136561274845874 1102 92-93 1.3300635091501647 1102 94-95 1.4637638916775906 1102 96-97 1.344814729011965 1102 98-99 1.7186972804521368 1102 100-101 2.1111989548312096 1103 1 0.05532766752324747 1103 2 0.038768206177891784 1103 3 0.0345356189732513 1103 4 -0.01960520716569647 1103 5 0.2349861857904969 1103 6 0.19223357514691486 1103 7 -0.02554288551417727 1103 8 -0.07047676362890343 1103 9 0.11400803150858252 1103 10-11 0.25592868858560536 1103 12-13 0.3544868900929359 1103 14-15 0.4369976375909346 1103 16-17 0.5262672673157667 1103 18-19 0.20181889728269198 1103 20-21 0.2247154262813993 1103 22-23 0.28648967194865094 1103 24-25 0.17894604082313492 1103 26-27 0.2975533790114895 1103 28-29 0.03978784868643004 1103 30-31 0.22395126429935885 1103 32-33 0.21286968680668394 1103 34-35 0.19656445443892778 1103 36-37 0.693720412750082 1103 38-39 0.4945543971046362 1103 40-41 0.8628433853563635 1103 42-43 0.829491262793006 1103 44-45 0.5991702944365827 1103 46-47 0.38184306574396487 1103 48-49 0.8010152453089674 1103 50-51 0.5170101992623373 1103 52-53 0.3722845672950328 1103 54-55 0.9138112301315289 1103 56-57 1.1237792179843638 1103 58-59 0.9318340369564879 1103 60-61 0.8824248163732022 1103 62-63 0.8335718526190234 1103 64-65 0.8046281812982059 1103 66-67 0.6632878219375797 1103 68-69 0.905803349952869 1103 70-71 1.2707289642803055 1103 72-73 1.3065588916247322 1103 74-75 1.2037585129011745 1103 76-77 1.3477561027188365 1103 78-79 1.2954191191405684 1103 80-81 0.9550426616373606 1103 82-83 1.0672901031998414 1103 84-85 1.5124435166674033 1103 86-87 1.6092409247407566 1103 88-89 1.7859799994134384 1103 90-91 1.6032384463722096 1103 92-93 1.4770228772181184 1103 94-95 1.7199046696898215 1103 96-97 2.142547781005689 1103 98-99 2.3278516385108965 1103 100-101 2.8126678722147496 1104 1 -0.028584171936557823 1104 2 -0.04931646435812809 1104 3 0.0033911252135290226 1104 4 0.1528844555936857 1104 5 -0.019822053654138472 1104 6 -0.10270971228527515 1104 7 -0.09530503414283231 1104 8 -0.008036036797598456 1104 9 0.17352777658653906 1104 10-11 0.14165001933936594 1104 12-13 0.07486927315881786 1104 14-15 0.30595177438392085 1104 16-17 0.5873044055123486 1104 18-19 0.45507180878073683 1104 20-21 0.175020801646518 1104 22-23 -0.08718735092605101 1104 24-25 0.18072897773751606 1104 26-27 0.24599994780630396 1104 28-29 -0.070905979317061 1104 30-31 0.4864033715790512 1104 32-33 0.08635703554156038 1104 34-35 0.013775485886903027 1104 36-37 -0.10099526571431738 1104 38-39 0.1186392193799719 1104 40-41 0.3052772839186346 1104 42-43 0.6099813807703214 1104 44-45 0.545189034241595 1104 46-47 0.6675302551525633 1104 48-49 0.24288012145175486 1104 50-51 0.10427927099709677 1104 52-53 0.09219276501135454 1104 54-55 -0.009219003926354219 1104 56-57 -0.1819754954180155 1104 58-59 0.4097369996665563 1104 60-61 0.5546299679228355 1104 62-63 0.5829554116645852 1104 64-65 0.46431483961921316 1104 66-67 0.75598157407142 1104 68-69 0.8937780094877823 1104 70-71 0.44221335409860174 1104 72-73 0.6533590888858356 1104 74-75 0.6684411880652412 1104 76-77 0.5702818035647859 1104 78-79 0.6636145593742491 1104 80-81 0.7448647472942049 1104 82-83 0.9804383145727016 1104 84-85 0.7316878548943322 1104 86-87 0.7687568763359174 1104 88-89 0.6551048429322712 1104 90-91 0.7259386474268013 1104 92-93 1.0192633081577611 1104 94-95 0.6119800841278114 1104 96-97 0.8474527542616386 1104 98-99 1.2019269770792356 1104 100-101 1.698332300726566 1105 1 0.12750445041892888 1105 2 0.1672419231148048 1105 3 0.04553671908326251 1105 4 0.12140019772262178 1105 5 -0.08528932002095502 1105 6 0.1738186901801555 1105 7 0.09776509745803708 1105 8 0.3247975464107995 1105 9 0.3994148330583016 1105 10-11 0.4510786383631924 1105 12-13 0.3336565461889691 1105 14-15 0.6076342664712087 1105 16-17 0.6124584951341987 1105 18-19 0.6049135545745088 1105 20-21 0.7381559007636227 1105 22-23 0.7295709365968506 1105 24-25 -0.0025960264270707967 1105 26-27 0.056844525517441014 1105 28-29 0.641404532093901 1105 30-31 0.4461734865215803 1105 32-33 0.054956069357984916 1105 34-35 0.04209465963336356 1105 36-37 0.16112367481542123 1105 38-39 -0.3886904491191103 1105 40-41 0.13586199156481626 1105 42-43 0.015362023782145684 1105 44-45 0.21585370190776842 1105 46-47 0.18310580071311477 1105 48-49 -0.04389315857489606 1105 50-51 0.34857379040406045 1105 52-53 0.15857623994055814 1105 54-55 0.41790076952025146 1105 56-57 0.2705482427129198 1105 58-59 0.226328703814481 1105 60-61 0.2096357983409618 1105 62-63 0.456018879930447 1105 64-65 -0.3152120635958511 1105 66-67 -0.03437324851729073 1105 68-69 0.0709394287781393 1105 70-71 0.42263980753854824 1105 72-73 0.03791681002526559 1105 74-75 -0.11058596502487816 1105 76-77 0.0856907657503605 1105 78-79 0.20334469430678226 1105 80-81 0.46042363451725876 1105 82-83 0.16826106986172462 1105 84-85 0.13015528784452357 1105 86-87 0.07793562029726786 1105 88-89 0.31494159122479104 1105 90-91 0.48239375320724065 1105 92-93 0.6508641743913159 1105 94-95 0.42474037065121806 1105 96-97 0.17062450172124244 1105 98-99 0.32311638237658613 1105 100-101 1.566314976055569 1106 1 -0.1197114153324037 1106 2 -0.07926201789714682 1106 3 0.06717414598384863 1106 4 0.2881939938070346 1106 5 0.31943989431924535 1106 6 0.14564681120736367 1106 7 0.09519708321953857 1106 8 0.19914282990939824 1106 9 0.2947618963687546 1106 10-11 -0.12451251342933034 1106 12-13 -0.07894197910977852 1106 14-15 -0.11706697939708022 1106 16-17 0.13844374812173754 1106 18-19 0.10397279688317695 1106 20-21 0.3469405435299606 1106 22-23 0.3607557827702621 1106 24-25 -0.1728604556159823 1106 26-27 0.14361035312995085 1106 28-29 0.3528588926329306 1106 30-31 0.3528986327204109 1106 32-33 0.23577224021992294 1106 34-35 0.17169038808492587 1106 36-37 -0.13999506406075568 1106 38-39 0.36067390379214714 1106 40-41 0.6473926297544921 1106 42-43 0.91664603090139 1106 44-45 0.4860037146207077 1106 46-47 0.4988571355720026 1106 48-49 0.34461434206075126 1106 50-51 0.073567433933178 1106 52-53 0.1423036744689128 1106 54-55 -0.8002151725087359 1106 56-57 -0.6245445617620362 1106 58-59 -1.1927534217893836 1106 60-61 -0.7649001792842611 1106 62-63 -0.497998409670366 1106 64-65 -0.8164070801226728 1106 66-67 -0.6146173369993662 1106 68-69 -0.604054214750974 1106 70-71 -0.3348989669338671 1106 72-73 -0.4292583743123899 1106 74-75 -0.7644888381497204 1106 76-77 -0.29122253198673675 1106 78-79 -0.16733926196070215 1106 80-81 -0.16341313131035307 1106 82-83 -0.8954943331385294 1106 84-85 -0.26341196361746455 1106 86-87 -0.2622372757108664 1106 88-89 0.01393726882458779 1106 90-91 0.029215517763560683 1106 92-93 -0.1855965426186046 1106 94-95 0.14133075966723752 1106 96-97 0.6935205098575246 1106 98-99 0.8958471222697959 1106 100-101 0.6452369185930742 1107 1 0.258910396930208 1107 2 0.05448730386085643 1107 3 0.32159628380262006 1107 4 0.22660962050264288 1107 5 0.07076924863286393 1107 6 0.14119754370872073 1107 7 -0.030901668815189964 1107 8 0.35985905345796 1107 9 -0.08011602007183427 1107 10-11 0.05592698738455937 1107 12-13 -0.11407491454114194 1107 14-15 0.4369210835437727 1107 16-17 0.08977310243535896 1107 18-19 0.18031571603673058 1107 20-21 0.7512541626292588 1107 22-23 0.5262739596557751 1107 24-25 0.46843091714581675 1107 26-27 0.6380487687566543 1107 28-29 0.13967386821622085 1107 30-31 -0.22192502436043782 1107 32-33 0.20462736772941525 1107 34-35 -0.04247130480709416 1107 36-37 -0.1083618573325964 1107 38-39 0.011379275468769379 1107 40-41 0.20756625970565779 1107 42-43 0.18536551001153612 1107 44-45 0.33570528814214384 1107 46-47 0.5432955511986961 1107 48-49 0.20720251352032193 1107 50-51 -0.15969355358717507 1107 52-53 -0.464587264218018 1107 54-55 -0.2677409457046096 1107 56-57 -0.22077353626013263 1107 58-59 -0.3267740403460877 1107 60-61 -0.11726046143293445 1107 62-63 0.1692018073670738 1107 64-65 0.31609427636131926 1107 66-67 0.35125352572445223 1107 68-69 0.26219646349102277 1107 70-71 0.7026213803260397 1107 72-73 1.0423368508639541 1107 74-75 1.1560483295117017 1107 76-77 0.8291789872753377 1107 78-79 1.0230568313653983 1107 80-81 1.205306347799791 1107 82-83 1.2055637243763933 1107 84-85 1.3498001904791153 1107 86-87 1.4649195338387813 1107 88-89 1.2587012406097209 1107 90-91 1.147690829754879 1107 92-93 0.6262862571643595 1107 94-95 1.2988999403509034 1107 96-97 0.9470202385607251 1107 98-99 1.4309529280212878 1107 100-101 1.5667507547298065 1108 1 -0.23299331117148014 1108 2 0.03192563710423002 1108 3 -0.037069447292537916 1108 4 0.03826288330827765 1108 5 -0.2973691439833175 1108 6 -0.20888611797423096 1108 7 -0.3216395108835215 1108 8 -0.00873812903245863 1108 9 -0.16299506147087328 1108 10-11 -0.312332849610101 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-0.5232566293263119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1189.0 3 229.0 4 188.0 5 206.0 6 205.0 7 259.0 8 316.0 9 391.0 10 565.0 11 758.0 12 1069.0 13 1565.0 14 1758.0 15 2334.0 16 3044.0 17 4191.0 18 3690.0 19 2396.0 20 2220.0 21 2252.0 22 2458.0 23 2585.0 24 2776.0 25 3151.0 26 3493.0 27 3900.0 28 4543.0 29 5345.0 30 6374.0 31 7892.0 32 10013.0 33 12747.0 34 17368.0 35 26553.0 36 34774.0 37 50256.0 38 49078.0 39 7399.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07519228561589 19.97689702674779 14.292632877970382 20.65527780966594 2 14.354935315995796 22.29060638339843 42.51821086949504 20.83624743111073 3 19.213748287560698 28.748897224951765 29.075246914022994 22.962107573464543 4 11.059514546047842 18.463601711173187 39.18378616789722 31.293097574881756 5 11.784427272890259 40.62678164834968 33.59700234863833 13.991788730121735 6 27.9422201512599 38.4569339402846 19.551955267213877 14.048890641241622 7 26.371928818479955 35.65240766294511 20.957330513790076 17.01833300478486 8 25.11076221610461 36.7995813259922 20.098144643266806 17.991511814636386 9 26.932314332212528 18.039832811166953 20.994673185979654 34.03317967064087 10-11 23.486290493140764 29.72663433309316 27.014306351647328 19.772768822118746 12-13 25.2665027385083 27.458922375476728 27.72703810971239 19.547536776302586 14-15 21.612757537958622 28.748906763014897 27.722160092907224 21.91617560611926 16-17 19.20906992415444 32.32934749881715 28.163935366395688 20.297647210632714 18-19 18.942401264559187 30.27379068462905 32.08030380962793 18.70350424118384 20-21 20.93495425042739 29.15787096936051 31.765041090391694 18.142133689820405 22-23 19.698109961941213 30.173235570272578 31.351911181829255 18.776743285956954 24-25 18.997054164665744 30.14643166880497 32.075201227073016 18.781312939456274 26-27 19.070301733484584 30.624595660643468 31.579598178524005 18.725504427347946 28-29 18.67037560132776 31.001985905092376 31.650810493034992 18.67682800054487 30-31 19.011577890888233 31.116567495877838 31.68757617033479 18.18427844289913 32-33 18.531343818613614 31.2941703634001 31.503338262311242 18.671147555675045 34-35 18.718065897828794 31.644040610410674 31.419303386291748 18.218590105468785 36-37 18.646874148830943 31.52640595209083 31.194898003067795 18.631821896010436 38-39 18.695412206162214 31.60743021184634 31.364439490651307 18.33271809134014 40-41 19.09259508130628 31.67585002015858 31.00174707700578 18.229807821529363 42-43 18.41154523004799 32.126254896830545 30.82646671947299 18.635733153648477 44-45 18.701156737480623 32.288118128791204 30.472640604979972 18.538084528748197 46-47 18.643855181596457 32.16444483471111 29.88837377670813 19.303326206984302 48-49 18.822605909193552 32.23012376832061 29.893114065429106 19.05415625705673 50-51 19.042267557113718 32.71819358261549 29.14511347464341 19.094425385627382 52-53 18.737228527794308 32.81470700002151 29.192986355586463 19.255078116597716 54-55 18.80930832713555 32.55315907848637 29.125709011729494 19.511823582648592 56-57 18.974620835460954 32.67112795291462 28.777267148628916 19.57698406299551 58-59 19.00369631783675 32.61557040293985 28.82106632408985 19.559666955133544 60-61 19.10947060984241 32.16764170974476 29.222444207527392 19.50044347288544 62-63 19.296837200967655 32.09730310904041 29.333213869724933 19.272645820267 64-65 19.179360932835955 32.09400744634724 29.398200406359855 19.328431214456952 66-67 19.02886457177977 32.088788705744065 29.40086617009115 19.481480552385015 68-69 19.209032461528537 32.0796809036762 29.394741934790243 19.316544700005018 70-71 19.125757472594813 31.76224588160672 29.459278344973892 19.652718300824574 72-73 18.92758271589453 31.71792827257497 29.46146196367937 19.893027047851135 74-75 19.021799507602772 31.75514168067287 29.372892349924562 19.850166461799795 76-77 18.908834803739722 31.543398045551235 29.67138522855366 19.876381922155378 78-79 18.748006236670907 31.61597878098174 29.608415921432286 20.02759906091507 80-81 18.60320088596911 31.835930849967653 29.421736707327344 20.139131556735894 82-83 18.776230668602047 31.74936831353153 29.49877246743007 19.975628550436355 84-85 18.919049675406704 31.350713065977644 29.545784408412366 20.184452850203286 86-87 18.719414843577812 31.533688245086218 29.41138524888321 20.335511662452763 88-89 19.192096581409476 30.994718988167453 29.52617837168503 20.28700605873804 90-91 18.989038479113617 31.370521626469642 29.584458545309232 20.05598134910751 92-93 18.79725116699967 31.447796364157654 29.58740625923968 20.167546209602996 94-95 18.677561552521237 31.76988137476257 29.11729921513816 20.435257857578037 96-97 18.95455050401889 31.592470355518763 28.77566231988447 20.67731682057787 98-99 19.447560341892594 31.38925224434211 28.58548210797929 20.57770530578601 100-101 19.825014379066232 30.930132968287218 28.784113326751516 20.46073932589504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1277.0 1 774.0 2 512.0 3 744.0 4 645.5 5 618.5 6 894.0 7 1428.0 8 1797.5 9 1707.0 10 1314.5 11 1037.0 12 1035.5 13 1027.5 14 940.0 15 828.5 16 746.0 17 729.0 18 739.5 19 786.5 20 853.5 21 968.0 22 1132.5 23 1340.0 24 1634.0 25 2017.0 26 2462.0 27 2987.5 28 3555.5 29 4233.0 30 5058.0 31 5865.0 32 6641.0 33 7473.0 34 8420.5 35 9297.0 36 9992.0 37 10516.5 38 10821.0 39 10805.0 40 10682.5 41 10621.0 42 10439.5 43 10346.0 44 10356.0 45 10137.0 46 9878.5 47 9712.5 48 10024.0 49 9554.5 50 8382.0 51 7546.5 52 6491.5 53 5597.0 54 4961.0 55 4301.0 56 3577.0 57 2898.5 58 2291.5 59 1790.5 60 1386.5 61 1066.0 62 762.5 63 497.5 64 300.5 65 214.0 66 161.5 67 114.0 68 87.0 69 69.0 70 52.5 71 34.0 72 24.5 73 30.5 74 36.0 75 24.5 76 15.5 77 14.0 78 10.5 79 7.0 80 7.0 81 5.0 82 3.0 83 2.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2790378203568822 2 0.2557846686604754 3 0.24684114877724195 4 0.23575118412203253 5 0.2314582945780805 6 0.1938955110685002 7 0.18852939913856015 8 0.19890388220311092 9 0.20248129015640426 10-11 0.2021235493610749 12-13 0.19604195584047623 14-15 0.19461099265915888 16-17 0.19461099265915888 18-19 0.19371664067083552 20-21 0.18352102800394945 22-23 0.17529298971137472 24-25 0.17672395289269208 26-27 0.18870826953622483 28-29 0.20248129015640426 30-31 0.19747291902179356 32-33 0.20373338294005697 34-35 0.19300115908017687 36-37 0.18030136084598544 38-39 0.18209006482263213 40-41 0.17690282329035675 42-43 0.1878139175479015 44-45 0.18405763919694346 46-47 0.1862040839689195 48-49 0.19425325186382952 50-51 0.20426999413305094 52-53 0.21106706924430835 54-55 0.1912124551035302 56-57 0.16617059943047666 58-59 0.16831704420245266 60-61 0.17332541533706336 62-63 0.18137458323197345 64-65 0.16778043300945866 66-67 0.1743986377230514 68-69 0.17672395289269208 70-71 0.17135784096275203 72-73 0.17511411931371007 74-75 0.1749352489160454 76-77 0.18870826953622483 78-79 0.19210680709185354 80-81 0.18441537999227278 82-83 0.1849519911852668 84-85 0.1754718601090394 86-87 0.17726056408568608 88-89 0.18369989840161413 90-91 0.1826266760156261 92-93 0.1804802312436501 94-95 0.17958587925532676 96-97 0.1699268777814347 98-99 0.17779717527868008 100-101 0.17189445215574603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 279532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.11771720150787 #Duplication Level Percentage of deduplicated Percentage of total 1 92.3242068513279 77.66101522773515 2 6.056879478082792 10.189817501219698 3 0.9341585499079144 2.3573785416757387 4 0.26001768469106457 0.8748837627293525 5 0.1078869089081424 0.4537600246639981 6 0.05580828679286764 0.2816679411565852 7 0.04338312958421077 0.2554502877976708 8 0.03077457669908678 0.20709497118159165 9 0.027992605399628417 0.21192066582654112 >10 0.1354278712257265 2.1233580518160955 >50 0.013651815203297833 0.7891383149054703 >100 0.007679146051853776 1.433296239077785 >500 4.2661922510298756E-4 0.25263909672277435 >1k 0.0017064769004119503 2.9085793734915995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2740 0.9802097792023812 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2021 0.7229941473605884 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1765 0.6314125037562784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.5648727158250219 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 704 0.25184951991185267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 456 0.1631298026701773 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 452 0.16169883948885994 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 376 0.1345105390438304 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 374 0.13379505745317172 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 335 0.11984316643532762 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 319 0.11411931371005823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.788703976646681E-4 0.0 24-25 0.0 0.0 0.0 8.943519883233404E-4 0.0 26-27 0.0 0.0 0.0 0.001967574374311349 0.0 28-29 0.0 0.0 0.0 0.004471759941616702 0.0 30-31 0.0 0.0 0.0 0.010374483064550749 0.0 32-33 0.0 0.0 0.0 0.01395189101784411 0.0 34-35 0.0 0.0 0.0 0.0168138173804788 0.0 36-37 0.0 0.0 0.0 0.020927836526766167 0.0 38-39 1.788703976646681E-4 0.0 0.0 0.028619263626346897 0.0 40-41 3.577407953293362E-4 0.0 0.0 0.04775839617646638 0.0 42-43 3.577407953293362E-4 0.0 0.0 0.07745088218880128 0.0 44-45 3.577407953293362E-4 0.0 0.0 0.10428144183850149 0.0 46-47 3.577407953293362E-4 0.0 0.0 0.16008900590987796 0.0 48-49 3.577407953293362E-4 0.0 0.0 0.21893736674155373 0.0 50-51 3.577407953293362E-4 0.0 0.0 0.25739450223945737 0.0 52-53 3.577407953293362E-4 0.0 0.0 0.31767382625245055 0.0 54-55 3.577407953293362E-4 0.0 0.0 0.37419687191448564 0.0 56-57 3.577407953293362E-4 0.0 0.0 0.48563312965957384 0.0 58-59 3.577407953293362E-4 0.0 0.0 0.6026143697322668 0.0 60-61 3.577407953293362E-4 0.0 0.0 0.7364094271854384 0.0 62-63 3.577407953293362E-4 0.0 0.0 0.8795057453171731 0.0 64-65 3.577407953293362E-4 0.0 0.0 1.0209922298699254 0.0 66-67 3.577407953293362E-4 0.0 0.0 1.219538371277707 0.0 68-69 3.577407953293362E-4 0.0 0.0 1.473892076756865 0.0 70-71 3.577407953293362E-4 0.0 0.0 1.6575919751584791 0.0 72-73 3.577407953293362E-4 0.0 0.0 1.8513086158293146 0.0 74-75 3.577407953293362E-4 0.0 0.0 2.0777585392727844 0.0 76-77 3.577407953293362E-4 0.0 0.0 2.329608059184637 0.0 78-79 3.577407953293362E-4 0.0 0.0 2.590579969377388 0.0 80-81 3.577407953293362E-4 0.0 0.0 2.9728260091867837 0.0 82-83 3.577407953293362E-4 0.0 0.0 3.371885866376658 0.0 84-85 3.577407953293362E-4 0.0 0.0 3.8394530858721003 0.0 86-87 3.577407953293362E-4 0.0 0.0 4.2621238355537105 0.0 88-89 3.577407953293362E-4 0.0 0.0 4.891568764935679 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTCG 15 6.610941E-4 95.006096 9 GGTATCA 1690 0.0 33.185658 1 GTAGCCC 115 1.0310214E-7 33.0456 3 GTATCAA 2485 0.0 32.32328 1 TAGCCCT 190 1.2732926E-11 30.007303 4 CTATTAG 85 6.372439E-4 27.958006 1 TATCAAC 2925 0.0 27.613558 2 ATCAACG 2955 0.0 27.489073 3 TAGGACG 225 3.6379788E-12 27.451128 4 TCAACGC 2990 0.0 27.172165 4 CAACGCA 3000 0.0 26.92322 5 AACGCAG 3150 0.0 25.787369 6 GTCTTAA 150 1.0497715E-6 25.348593 1 GGTGACC 95 0.0012237685 25.001604 8 AGGACGT 230 1.3278623E-10 24.788643 5 TATAGCC 115 1.2762424E-4 24.788643 2 GACGTGG 115 1.2777977E-4 24.7842 7 GGACGTG 215 1.6461854E-9 24.303885 6 GTAGGAC 255 1.8189894E-11 24.21724 3 GAGTACT 1755 0.0 24.085546 12-13 >>END_MODULE