FastQCFastQC Report
Thu 26 May 2016
SRR937597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279532
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43121.5425783094600976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34591.2374254110441738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.9458666628507648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.5999313137672968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11410.4081822474707726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.29406293376071435No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.27152526365496615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.26830559649700214No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.1434540589270638No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.1334373166578424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.040.620211
GTATCAA26550.038.3164141
TAGGACG905.6821955E-736.940484
AGGACGT958.6759246E-734.9962435
GTATAAG1001.2865876E-633.2761961
GGACGTG1001.2956189E-633.2464336
CGAAATC300.00395130731.66326978-79
GACGTGG600.00394795631.6632697
TATCAAC33400.030.0042632
ATCAACG33550.029.8701173
TCAACGC33500.029.7729244
CAACGCA34000.029.3350855
GTCTTAG1303.0022056E-729.2537981
TAGACTG1004.943503E-528.4969435
AACGCAG35250.028.1600976
CCCTAGG856.3655636E-427.963191
CTATGCC700.008385288527.1399444
GTAGGAC1658.371353E-825.9063113
ACGCAGA39450.025.1620667
CGCAGAG39650.025.0351438
Produced by FastQC (version 0.11.5)