##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279532 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.225816722235734 33.0 31.0 34.0 31.0 34.0 2 32.3857840962752 34.0 31.0 34.0 31.0 34.0 3 32.44423894223202 34.0 31.0 34.0 31.0 34.0 4 36.06672939055278 37.0 35.0 37.0 35.0 37.0 5 36.01155860509709 37.0 35.0 37.0 35.0 37.0 6 36.07358370419129 37.0 35.0 37.0 35.0 37.0 7 36.03016828127012 37.0 35.0 37.0 35.0 37.0 8 36.00443598586208 37.0 35.0 37.0 35.0 37.0 9 37.68464791150924 39.0 37.0 39.0 35.0 39.0 10-11 37.612734856832134 39.0 37.0 39.0 35.0 39.0 12-13 37.503121288439246 39.0 37.0 39.0 35.0 39.0 14-15 38.651075726571555 40.0 38.0 41.0 34.0 41.0 16-17 38.64801883147547 40.0 38.0 41.0 34.5 41.0 18-19 38.70032053575262 40.0 38.0 41.0 34.0 41.0 20-21 38.647868580341424 40.0 38.0 41.0 34.0 41.0 22-23 38.56904933961049 40.0 38.0 41.0 34.0 41.0 24-25 38.562874733483106 40.0 38.0 41.0 34.0 41.0 26-27 38.3547643919122 40.0 38.0 41.0 34.0 41.0 28-29 38.2713392384414 40.0 38.0 41.0 34.0 41.0 30-31 38.13881952692357 40.0 37.0 41.0 34.0 41.0 32-33 37.876650973770445 40.0 36.5 41.0 33.5 41.0 34-35 37.67615514502812 40.0 36.0 41.0 33.0 41.0 36-37 37.46656017915659 40.0 35.5 41.0 33.0 41.0 38-39 37.2902494168825 40.0 35.0 41.0 33.0 41.0 40-41 37.05217828370276 40.0 35.0 41.0 33.0 41.0 42-43 36.762045132578734 40.0 35.0 41.0 31.5 41.0 44-45 36.31641278994891 39.0 35.0 41.0 30.5 41.0 46-47 35.77943312393572 39.0 35.0 41.0 29.0 41.0 48-49 35.30066504013852 39.0 35.0 41.0 26.0 41.0 50-51 35.020255283831546 39.0 35.0 41.0 24.0 41.0 52-53 34.76726814819055 39.0 35.0 40.0 23.5 41.0 54-55 34.53765758482034 39.0 35.0 40.0 23.0 41.0 56-57 34.26720733225534 38.0 34.5 40.0 21.5 41.0 58-59 34.04190039065295 38.0 34.0 40.0 20.0 41.0 60-61 33.75609053704048 38.0 34.0 40.0 18.0 41.0 62-63 33.366594880013736 38.0 33.5 40.0 13.0 41.0 64-65 32.98714279581587 37.0 33.0 40.0 8.0 41.0 66-67 32.60901792996866 37.0 32.5 39.5 6.0 41.0 68-69 32.163412417898485 36.0 32.0 39.0 2.0 40.5 70-71 31.76102557131205 36.0 31.5 39.0 2.0 40.0 72-73 31.30033234119886 35.0 31.0 38.5 2.0 40.0 74-75 30.816237496959204 35.0 31.0 38.0 2.0 39.5 76-77 29.478464004121175 33.5 28.5 36.0 2.0 39.0 78-79 30.039558977147518 34.5 30.0 36.5 2.0 39.0 80-81 29.83742111815463 34.5 30.0 36.0 2.0 39.0 82-83 29.40784060501123 34.0 30.0 36.0 2.0 37.5 84-85 28.949517407667102 34.0 29.0 35.5 2.0 37.0 86-87 28.55813645664897 34.0 29.0 35.0 2.0 36.5 88-89 28.143089163315828 34.0 29.0 35.0 2.0 36.0 90-91 27.604199161455576 34.0 27.0 35.0 2.0 36.0 92-93 27.153810654951847 34.0 26.0 35.0 2.0 35.5 94-95 26.64277256271196 33.0 25.5 35.0 2.0 35.0 96-97 26.15037455461271 33.0 25.0 35.0 2.0 35.0 98-99 25.397287251549017 32.5 21.0 35.0 2.0 35.0 100-101 23.142903138102255 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3811010188422159 1101 2 0.2551771586428728 1101 3 0.32109206647176336 1101 4 0.19032098806172826 1101 5 0.22069885603183792 1101 6 0.207055546270297 1101 7 0.2275649181597288 1101 8 0.26381891871650964 1101 9 0.4824944295733644 1101 10-11 0.38762157162568656 1101 12-13 0.34242924747891124 1101 14-15 0.5489098535465118 1101 16-17 0.4514014806002393 1101 18-19 0.3825189811292091 1101 20-21 0.21383823931377322 1101 22-23 0.35186521154225403 1101 24-25 0.1356208487666919 1101 26-27 0.23418610545039087 1101 28-29 0.33893237462611125 1101 30-31 0.7245356556542362 1101 32-33 0.867668104738712 1101 34-35 0.7528499647495579 1101 36-37 0.7747666163286695 1101 38-39 1.089805159118228 1101 40-41 1.201404098009796 1101 42-43 1.0886455970451223 1101 44-45 1.4177621360373323 1101 46-47 1.361824448936531 1101 48-49 1.7960140434481957 1101 50-51 2.061176925150768 1101 52-53 1.836244349354864 1101 54-55 1.7643264853233376 1101 56-57 1.8724902064927065 1101 58-59 1.789623744145942 1101 60-61 2.0336010648769047 1101 62-63 1.6378337363397577 1101 64-65 2.0238026878542144 1101 66-67 2.105365773835672 1101 68-69 2.417221618288668 1101 70-71 2.1279061122052205 1101 72-73 2.3556826728050986 1101 74-75 2.4634427287043046 1101 76-77 2.2029927993637344 1101 78-79 2.332262746577811 1101 80-81 2.5260767625619565 1101 82-83 2.2729020576991523 1101 84-85 2.4330052432216753 1101 86-87 2.4687226711414745 1101 88-89 2.8120547543384475 1101 90-91 2.7048125346788865 1101 92-93 2.6528874812615832 1101 94-95 2.3842153531863346 1101 96-97 2.57790382760205 1101 98-99 2.908696309089212 1101 100-101 2.959669280944368 1102 1 0.21916674948775494 1102 2 0.27798940535502936 1102 3 0.26698324747942337 1102 4 0.24037720162096576 1102 5 0.11127850933797845 1102 6 0.2379468580071631 1102 7 0.1269949388149456 1102 8 0.20526530666850817 1102 9 0.3273450060891747 1102 10-11 0.2177455029144184 1102 12-13 0.18580258394974436 1102 14-15 0.3158785046825443 1102 16-17 0.2744594982225337 1102 18-19 0.29606775619653547 1102 20-21 0.05070126125013985 1102 22-23 0.22985564216426013 1102 24-25 0.07421733999476032 1102 26-27 0.10932010288810545 1102 28-29 0.12662814161436842 1102 30-31 0.2383341235079257 1102 32-33 0.4618127435339119 1102 34-35 0.4428321855773376 1102 36-37 0.39848220186479466 1102 38-39 0.5115964107196618 1102 40-41 0.4843217124632133 1102 42-43 0.8435087235648524 1102 44-45 0.8484495102104717 1102 46-47 0.7943093497130675 1102 48-49 0.5671660292248575 1102 50-51 0.7800764466818677 1102 52-53 0.6234368088941764 1102 54-55 0.6492324910231275 1102 56-57 1.0104029839654487 1102 58-59 0.6439325788257904 1102 60-61 0.11129343290577509 1102 62-63 0.20528347095946486 1102 64-65 0.32928416352550727 1102 66-67 0.11562148016557927 1102 68-69 0.054604942470962925 1102 70-71 0.13668457849015425 1102 72-73 0.4096934448918006 1102 74-75 0.30714288819393687 1102 76-77 -0.03316297755994668 1102 78-79 0.29902484556595965 1102 80-81 -0.01673051644032597 1102 82-83 0.2995642795727633 1102 84-85 0.4771263507595229 1102 86-87 0.373394933018222 1102 88-89 0.6334525110252471 1102 90-91 0.5806001781541923 1102 92-93 0.7969314585669913 1102 94-95 0.930415055123742 1102 96-97 1.210227931453332 1102 98-99 0.7839871815759416 1102 100-101 1.6165051112055657 1103 1 0.12616236708230133 1103 2 0.1110522198331978 1103 3 0.1206325468355871 1103 4 0.11783216023157905 1103 5 0.07221558665226979 1103 6 0.027645500002513756 1103 7 -0.0035845125728144467 1103 8 0.014928240174974405 1103 9 0.08301237610486112 1103 10-11 -0.019688896263211575 1103 12-13 0.04924466586285803 1103 14-15 0.2755035655492861 1103 16-17 0.21031947766310566 1103 18-19 0.1347573628621177 1103 20-21 -0.014737249814082531 1103 22-23 0.2834860052005652 1103 24-25 0.045339452205986674 1103 26-27 0.03514383280207767 1103 28-29 -0.044596767761809986 1103 30-31 -0.05800575638170358 1103 32-33 -0.32603284430356183 1103 34-35 0.1855011246023892 1103 36-37 0.10147955078001303 1103 38-39 0.1474780316686406 1103 40-41 0.12463984263688843 1103 42-43 0.24704169981330182 1103 44-45 -0.338100292310763 1103 46-47 -0.5198847746173954 1103 48-49 -0.09473950559092259 1103 50-51 -0.3288344444072422 1103 52-53 -0.4309915847648611 1103 54-55 -0.2332811701790547 1103 56-57 -0.2339296460188578 1103 58-59 -0.20273733406714456 1103 60-61 -0.07708237188782618 1103 62-63 -0.40618382331094693 1103 64-65 -0.4790397016478991 1103 66-67 -0.378629994162182 1103 68-69 -0.43543996365899673 1103 70-71 -0.44946215093688835 1103 72-73 -0.1699067282402673 1103 74-75 0.30961813571869 1103 76-77 0.13677696725420674 1103 78-79 0.15217368504007567 1103 80-81 0.3595206332648111 1103 82-83 0.2700912503026238 1103 84-85 0.2301020581663309 1103 86-87 0.21831999929534263 1103 88-89 0.25338704546229707 1103 90-91 -0.007588968629338666 1103 92-93 0.5497719065462192 1103 94-95 0.9727242040714223 1103 96-97 1.0124380705000249 1103 98-99 0.9087910554059455 1103 100-101 1.7818462354819253 1104 1 -0.022086940607088934 1104 2 0.08142511919209738 1104 3 -0.06471702260411405 1104 4 -0.05803370221673276 1104 5 0.07528830771747863 1104 6 -0.1370295881959649 1104 7 -0.014547677854864105 1104 8 0.029836627565437368 1104 9 -0.05089756661354983 1104 10-11 -0.0187974278060139 1104 12-13 -0.08544294082885528 1104 14-15 -0.08216495992395068 1104 16-17 -0.26790593108466965 1104 18-19 -0.1744114850571563 1104 20-21 -0.3849811707578965 1104 22-23 -0.4952401432763551 1104 24-25 -0.07041867533090596 1104 26-27 0.025185940461120992 1104 28-29 -0.1114379346371237 1104 30-31 -0.0994116211060998 1104 32-33 -0.4137761013878887 1104 34-35 -0.279845410051081 1104 36-37 -0.27132497105147735 1104 38-39 -0.3589519216715189 1104 40-41 -0.11199761421224252 1104 42-43 -0.2926950324806157 1104 44-45 -0.6536232239142734 1104 46-47 -0.6009704693937721 1104 48-49 -0.5832642201313405 1104 50-51 -0.5072798751572165 1104 52-53 -0.6919642337539003 1104 54-55 -1.2696796376120076 1104 56-57 -1.4827821174729081 1104 58-59 -1.0683342385851802 1104 60-61 -1.574388999102272 1104 62-63 -2.06786206010014 1104 64-65 -2.006106201401316 1104 66-67 -1.8860272856154623 1104 68-69 -2.01297875202059 1104 70-71 -1.4878521968380305 1104 72-73 -1.5667998658298892 1104 74-75 -1.6689107516528061 1104 76-77 -1.8882600192030594 1104 78-79 -1.5425837217350846 1104 80-81 -1.2127452140095727 1104 82-83 -1.3487775331274428 1104 84-85 -1.413348493785417 1104 86-87 -1.0326568225247748 1104 88-89 -1.6530087665326931 1104 90-91 -1.5261770343324592 1104 92-93 -1.3115573298256962 1104 94-95 -1.1001106656224415 1104 96-97 -1.1737460651549227 1104 98-99 -0.5639338032868189 1104 100-101 -0.6108361362035204 1105 1 -0.011925354733357096 1105 2 0.17887639355492269 1105 3 0.011578163136348962 1105 4 0.001603146025807689 1105 5 0.024480378348833653 1105 6 0.08169438315169941 1105 7 -0.15664329671210453 1105 8 0.0896400591829618 1105 9 0.26161284484739156 1105 10-11 -0.17930050959845545 1105 12-13 -0.06770181139356879 1105 14-15 0.1639619766077871 1105 16-17 0.3431218883389562 1105 18-19 0.36623485843776393 1105 20-21 0.31608496284196974 1105 22-23 0.1658459803285055 1105 24-25 0.1792898037628845 1105 26-27 0.24799120449577572 1105 28-29 0.37698452080181966 1105 30-31 0.043850081375993 1105 32-33 -0.2707642739682612 1105 34-35 -0.13008695594479036 1105 36-37 0.15404567695784266 1105 38-39 0.1658690012003774 1105 40-41 -0.05527597503183301 1105 42-43 -0.06589176744647318 1105 44-45 -0.25157424840202935 1105 46-47 -0.6010537610812605 1105 48-49 -0.7362710500497087 1105 50-51 -1.0460105098398742 1105 52-53 -0.7852509237422467 1105 54-55 -0.9530879334640758 1105 56-57 -0.9804499502231963 1105 58-59 -0.923739869103251 1105 60-61 -1.2815354258888831 1105 62-63 -1.6761412538366045 1105 64-65 -1.5653265912064143 1105 66-67 -0.764338130193174 1105 68-69 -0.9028671411593585 1105 70-71 -0.8706607925401748 1105 72-73 -0.9469431275323679 1105 74-75 -0.9476880296804602 1105 76-77 -0.7597409454066231 1105 78-79 -0.39324172606625396 1105 80-81 -0.5205912909710904 1105 82-83 -0.5680012546000377 1105 84-85 -1.1802150605020607 1105 86-87 -0.9956753132793956 1105 88-89 -0.6604217267192674 1105 90-91 -0.822029108295478 1105 92-93 -0.8411258788845011 1105 94-95 -0.5204838123823947 1105 96-97 -0.1645839795310664 1105 98-99 0.5954031948807632 1105 100-101 1.330609807491303 1106 1 -0.12751142134917615 1106 2 -0.3213778652805743 1106 3 -0.3784294646287165 1106 4 -0.3484349927995183 1106 5 -0.3180571248294015 1106 6 -0.36806407095458127 1106 7 -0.11741115839529215 1106 8 -0.37972175113994666 1106 9 -0.4509983933931423 1106 10-11 -0.4767324953599612 1106 12-13 -0.4616377381670276 1106 14-15 -0.4221427780324447 1106 16-17 -0.5179765098303832 1106 18-19 -0.676811162411461 1106 20-21 -0.318697645853689 1106 22-23 -0.44382856836205775 1106 24-25 -0.0670107301806695 1106 26-27 -0.19332585627208942 1106 28-29 -0.3560437019289111 1106 30-31 -0.15345477496776994 1106 32-33 -0.32300175172061785 1106 34-35 -0.7842313749633547 1106 36-37 -0.12403721142252522 1106 38-39 -0.15517091743679856 1106 40-41 -0.9861557106026453 1106 42-43 -0.7075266517587053 1106 44-45 -0.6269747060679336 1106 46-47 -0.26185976158977553 1106 48-49 -0.9374787795183153 1106 50-51 -1.0462393427918144 1106 52-53 -1.6639948850949011 1106 54-55 -1.341175907021153 1106 56-57 -1.5179404155168683 1106 58-59 -1.1364528108779766 1106 60-61 -1.68003529875946 1106 62-63 -1.3157547804066567 1106 64-65 -1.9907906135811935 1106 66-67 -1.5408064749681571 1106 68-69 -1.6825391472615685 1106 70-71 -1.888840299278037 1106 72-73 -2.1014943606877203 1106 74-75 -1.7095237784727324 1106 76-77 -1.6395909326936788 1106 78-79 -1.5543401242355905 1106 80-81 -1.8569375915050337 1106 82-83 -2.0646577509132875 1106 84-85 -1.968430163476885 1106 86-87 -2.241085941298717 1106 88-89 -1.5695241930299701 1106 90-91 -1.7973405753689597 1106 92-93 -1.082519217303009 1106 94-95 -1.1346841683447657 1106 96-97 -1.7180291867520197 1106 98-99 -2.012834791389256 1106 100-101 -1.1432015324527605 1107 1 -0.05149405835405929 1107 2 0.05952284009109832 1107 3 0.1263996183881062 1107 4 0.05509186558355594 1107 5 0.20972366735724535 1107 6 0.14843077440624342 1107 7 0.13744505430139498 1107 8 0.08256799384106017 1107 9 -0.16141619046313593 1107 10-11 -0.11248941342832808 1107 12-13 0.15181858304838158 1107 14-15 -0.06001581113064702 1107 16-17 0.20730835180825125 1107 18-19 0.10143083433297306 1107 20-21 0.010168333527758477 1107 22-23 0.008206157628173116 1107 24-25 -0.06581702426640845 1107 26-27 -0.3428375220344364 1107 28-29 -0.046629703014097856 1107 30-31 -0.43533214881475146 1107 32-33 -0.24014770939832175 1107 34-35 -0.26781791864213744 1107 36-37 -0.19324502477032723 1107 38-39 -0.3794519809202441 1107 40-41 -0.15875473393417394 1107 42-43 0.13094323429664456 1107 44-45 0.23016039973781943 1107 46-47 0.2619872270157515 1107 48-49 0.4326243132651939 1107 50-51 0.06873624049629967 1107 52-53 -0.05420107066191093 1107 54-55 0.06568790198589625 1107 56-57 0.15050776679458266 1107 58-59 0.10858245770584318 1107 60-61 0.45306291068167326 1107 62-63 0.6909462505211792 1107 64-65 0.36981709124788864 1107 66-67 0.5063177789656521 1107 68-69 0.25001646858216375 1107 70-71 0.5569003409824056 1107 72-73 0.4970406365993121 1107 74-75 0.5760432318365467 1107 76-77 0.39619311505455457 1107 78-79 0.6126973147226309 1107 80-81 0.8330244269431475 1107 82-83 0.5383536435522593 1107 84-85 0.4592963693500245 1107 86-87 0.6382955020002505 1107 88-89 0.2430097190698639 1107 90-91 0.09245866500000588 1107 92-93 0.2806929367935709 1107 94-95 0.2937748658386319 1107 96-97 0.2462681545393579 1107 98-99 0.36030682553966287 1107 100-101 1.1961907627181567 1108 1 -0.09642847838968294 1108 2 -0.15397637726734814 1108 3 -0.19526605873177516 1108 4 -0.1530368114823446 1108 5 -0.33718085037363466 1108 6 -0.13089129608547267 1108 7 0.02976437205386162 1108 8 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-0.029818464306178072 2316 92-93 0.5029672260781695 2316 94-95 0.40625392067945043 2316 96-97 0.8603092212514625 2316 98-99 0.6802636572115794 2316 100-101 0.878619549176932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 8.0 6 16.0 7 57.0 8 98.0 9 161.0 10 301.0 11 551.0 12 908.0 13 1419.0 14 1666.0 15 2533.0 16 3499.0 17 4995.0 18 4610.0 19 2703.0 20 1992.0 21 1840.0 22 1973.0 23 2101.0 24 2281.0 25 2582.0 26 2953.0 27 3368.0 28 4102.0 29 4911.0 30 5936.0 31 7229.0 32 9242.0 33 11683.0 34 16323.0 35 24917.0 36 32978.0 37 55323.0 38 57753.0 39 6510.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24096230311403 19.80330192224413 15.585888988701313 20.369846785940528 2 14.600117338980867 21.577136070288912 42.871299171472316 20.951447419257903 3 18.93772448234907 27.79180916675014 30.812214701715725 22.458251649185065 4 11.17085403765593 18.386511692799726 39.712232274774536 30.730401994769814 5 11.613014017186975 39.035969575763644 34.99180720822571 14.359209198823672 6 27.79681753788475 38.345162628965554 19.371306326288224 14.486713506861468 7 25.416052544968014 35.226020634489075 21.909119528354534 17.448807292188373 8 24.95134725183521 35.240688007097575 21.088819884664368 18.719144856402846 9 26.435256070861296 16.818468010818084 21.968862241174534 34.777413677146086 10-11 23.310390223659542 28.231472604209895 28.23898516091181 20.219152011218753 12-13 25.320017458750126 26.187767426551613 29.22891712817871 19.263297986519557 14-15 21.59484423965771 27.06792066740123 29.89085328334502 21.446381809596037 16-17 18.702688779817695 30.152004063935433 30.58862670463489 20.55668045161198 18-19 18.412560994805606 27.247506546656552 35.68518094529428 18.654751513243564 20-21 20.11168687607658 26.04500744996539 35.938525711771305 17.90477996218673 22-23 18.804086903948456 27.158020513224123 35.587081033317 18.450811549510423 24-25 18.397178140606442 26.83646237282315 36.31713006024355 18.44922942632686 26-27 18.74012846632812 27.185242291930876 35.66845598928195 18.406173252459052 28-29 18.17313471280158 27.442867977447698 36.09548953950946 18.288507770241264 30-31 18.66387390352446 27.439434483350745 36.06420731794571 17.832484295179086 32-33 18.05982857061088 27.369138417068527 36.09962365668331 18.47140935563728 34-35 18.42382982985848 27.662843610033917 35.80287766695763 18.11044889314998 36-37 18.319533358733157 27.65933470349283 35.616097764278905 18.405034173495103 38-39 18.401113289355063 27.67804759383541 35.778193552079905 18.14264556472962 40-41 18.686444998148687 27.558253720958103 35.54447352087332 18.21082776001989 42-43 18.195285062805596 27.839312135139828 35.55238689893675 18.413015903117827 44-45 18.453522315870813 28.227358585063605 34.886882360516864 18.432236738548717 46-47 18.317580813645666 28.248107551192707 34.10396662993861 19.330345005223016 48-49 18.539916717942848 28.27833664839804 33.94387762402874 19.23786900963038 50-51 18.85293991385602 28.897049353920124 32.9922871084527 19.257723623771163 52-53 18.624522416038236 28.883812944492938 32.908218021550304 19.583446617918522 54-55 18.82664596539931 28.643053389236293 32.69446789634103 19.835832749023368 56-57 19.110334416095473 28.73606599602192 32.13174162528798 20.021857962594623 58-59 19.04129044259691 28.60942575447534 32.53616759440779 19.813116208519954 60-61 19.044510109754874 28.491729032811985 32.72970536468097 19.734055492752173 62-63 19.233754990484094 28.317867006281926 32.927178283702766 19.521199719531214 64-65 19.12088776955769 28.40444027875163 32.87530586838 19.599366083310677 66-67 19.071161799006912 28.36526766166306 32.88263955468425 19.680930984645766 68-69 19.44768398609104 28.221813602736002 32.73954323655252 19.590959174620437 70-71 19.190826095044574 27.993217234520557 32.93719502597198 19.878761644462887 72-73 18.86760728646452 28.034178555585765 32.78658615113833 20.311628006811386 74-75 19.075812429346193 27.899131405348943 32.578738749051986 20.446317416252878 76-77 18.66691470028476 27.971752786800796 33.19047550906515 20.170857003849292 78-79 18.536339309989554 27.717756822116968 33.32176638095102 20.424137486942463 80-81 18.53007884607129 28.076213099036963 33.02609361361132 20.367614441280427 82-83 18.444790658655645 28.05032706510715 33.11469369283962 20.390188583397578 84-85 18.440464776841292 27.611150065108824 33.15970980066683 20.788675357383056 86-87 18.166971938812015 27.58575046864044 33.01106850020749 21.236209092340054 88-89 18.554047479358356 27.144656067999374 33.12447233232689 21.176824120315384 90-91 18.20175634748417 27.524637701900357 33.17668483598419 21.09692111463128 92-93 17.753573932828665 27.411063092972338 33.37924471601723 21.456118258181768 94-95 17.69314425539831 27.551407352288827 33.04505387576378 21.71039451654909 96-97 17.742870225949087 27.334258689523917 32.86564686690611 22.05722421762088 98-99 18.04916091596281 26.961410362356947 32.78366263491348 22.20576608676676 100-101 17.922484503161982 26.638818572949184 32.85293880873369 22.585758115155148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 665.0 1 497.0 2 922.5 3 1214.0 4 658.0 5 454.5 6 776.5 7 1536.0 8 2269.0 9 2283.5 10 1582.0 11 987.5 12 827.0 13 763.5 14 741.0 15 709.5 16 676.5 17 712.5 18 741.5 19 775.5 20 863.5 21 1032.0 22 1243.0 23 1438.5 24 1657.5 25 2003.0 26 2436.5 27 2963.5 28 3561.5 29 4135.0 30 4792.0 31 5529.0 32 6377.5 33 7236.0 34 8152.0 35 9131.5 36 9897.0 37 10336.5 38 10657.5 39 10740.5 40 10677.5 41 10666.5 42 10482.5 43 10406.0 44 10439.0 45 10324.0 46 9986.0 47 9749.0 48 10093.5 49 9694.5 50 8632.5 51 7713.5 52 6613.0 53 5732.5 54 5047.0 55 4415.5 56 3655.5 57 2913.5 58 2348.0 59 1793.0 60 1309.5 61 987.0 62 700.5 63 434.5 64 273.5 65 204.0 66 155.5 67 108.0 68 72.5 69 54.5 70 42.5 71 32.5 72 22.5 73 20.5 74 23.0 75 17.0 76 12.5 77 9.5 78 8.0 79 6.0 80 3.0 81 3.0 82 2.0 83 1.0 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042571154644191006 2 0.0 3 0.0 4 3.5774079532933614E-4 5 0.006439334315928051 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005723852725269378 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7887039766466807E-4 22-23 0.0017887039766466808 24-25 0.0 26-27 0.0012520927836526766 28-29 0.0014309631813173445 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.943519883233404E-4 38-39 0.0 40-41 1.7887039766466807E-4 42-43 0.020927836526766167 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7887039766466807E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.01126883505287409 92-93 0.002861926362634689 94-95 0.0 96-97 0.0 98-99 3.5774079532933614E-4 100-101 0.014130761415508779 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 279532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.28295388150966 #Duplication Level Percentage of deduplicated Percentage of total 1 90.83589199422778 72.9257372775838 2 7.0234382268162765 11.277247345062463 3 1.260802287508976 3.03662795705355 4 0.37304425270846514 1.1979637814382376 5 0.1529353355577789 0.6139050245719184 6 0.08555252147713639 0.4121045481717483 7 0.04646999031016425 0.26115236622615823 8 0.032371775036705276 0.20791213778675321 9 0.02585399009212093 0.1868071224796877 >10 0.13571432604744757 2.033441275612834 >50 0.013962650108572647 0.7474290634539702 >100 0.010359385564424787 1.471715669210275 >500 0.001351224204055407 0.8428917014567938 >1k 0.0022520403400923448 4.785064729891786 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 1.5425783094600976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3459 1.2374254110441738 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2644 0.9458666628507648 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.5999313137672968 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1141 0.4081822474707726 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 822 0.29406293376071435 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 0.27152526365496615 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.26830559649700214 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.1434540589270638 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.1334373166578424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.577407953293362E-4 0.0 20-21 0.0 0.0 0.0 3.577407953293362E-4 0.0 22-23 0.0 0.0 0.0 5.366111929940042E-4 0.0 24-25 0.0 0.0 0.0 0.0012520927836526766 0.0 26-27 0.0 0.0 0.0 0.002325315169640685 0.0 28-29 0.0 0.0 0.0 0.0048295007369460385 0.0 30-31 0.0 0.0 0.0 0.010911094257544753 0.0 32-33 0.0 0.0 0.0 0.014667372608502784 0.0 34-35 0.0 0.0 0.0 0.017529298971137475 0.0 36-37 3.577407953293362E-4 0.0 0.0 0.02164331811742484 0.0 38-39 3.577407953293362E-4 0.0 0.0 0.02897700442167623 0.0 40-41 3.577407953293362E-4 0.0 0.0 0.04775839617646638 0.0 42-43 3.577407953293362E-4 0.0 0.0 0.07762975258646596 0.0 44-45 3.577407953293362E-4 0.0 0.0 0.10410257144083682 0.0 46-47 3.577407953293362E-4 0.0 0.0 0.16044674670520725 0.0 48-49 3.577407953293362E-4 0.0 0.0 0.21929510753688308 0.0 50-51 3.577407953293362E-4 0.0 0.0 0.2581099838301161 0.0 52-53 3.577407953293362E-4 0.0 0.0 0.31803156704777985 0.0 54-55 3.577407953293362E-4 0.0 0.0 0.37419687191448564 0.0 56-57 3.577407953293362E-4 0.0 0.0 0.4865274816478972 0.0 58-59 3.577407953293362E-4 0.0 0.0 0.6035087217205901 0.0 60-61 3.577407953293362E-4 0.0 0.0 0.7389136127527438 0.0 62-63 3.577407953293362E-4 0.0 0.0 0.882188801282143 0.0 64-65 3.577407953293362E-4 0.0 0.0 1.025642860209207 0.0 66-67 3.577407953293362E-4 0.0 0.0 1.2250833536053118 0.0 68-69 3.577407953293362E-4 0.0 0.0 1.4833722078330926 0.0 70-71 3.577407953293362E-4 0.0 0.0 1.66796645822303 0.0 72-73 3.577407953293362E-4 0.0 0.0 1.8627563212798535 0.0 74-75 3.577407953293362E-4 0.0 0.0 2.0895639855186525 0.0 76-77 3.577407953293362E-4 0.0 0.0 2.344990913383799 0.0 78-79 3.577407953293362E-4 0.0 0.0 2.60828813874619 0.0 80-81 3.577407953293362E-4 0.0 0.0 2.99643690167852 0.0 82-83 3.577407953293362E-4 0.0 0.0 3.403188185967975 0.0 84-85 3.577407953293362E-4 0.0 0.0 3.877910221370004 0.0 86-87 3.577407953293362E-4 0.0 0.0 4.309703361332513 0.0 88-89 3.577407953293362E-4 0.0 0.0 4.947734069802384 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1615 0.0 40.62021 1 GTATCAA 2655 0.0 38.316414 1 TAGGACG 90 5.6821955E-7 36.94048 4 AGGACGT 95 8.6759246E-7 34.996243 5 GTATAAG 100 1.2865876E-6 33.276196 1 GGACGTG 100 1.2956189E-6 33.246433 6 CGAAATC 30 0.003951307 31.663269 78-79 GACGTGG 60 0.003947956 31.663269 7 TATCAAC 3340 0.0 30.004263 2 ATCAACG 3355 0.0 29.870117 3 TCAACGC 3350 0.0 29.772924 4 CAACGCA 3400 0.0 29.335085 5 GTCTTAG 130 3.0022056E-7 29.253798 1 TAGACTG 100 4.943503E-5 28.496943 5 AACGCAG 3525 0.0 28.160097 6 CCCTAGG 85 6.3655636E-4 27.96319 1 CTATGCC 70 0.0083852885 27.139944 4 GTAGGAC 165 8.371353E-8 25.906311 3 ACGCAGA 3945 0.0 25.162066 7 CGCAGAG 3965 0.0 25.035143 8 >>END_MODULE