FastQCFastQC Report
Thu 26 May 2016
SRR937596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218877
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22851.0439653321271765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.7967945467088822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16220.7410554786478251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.5733813968575958No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.2704715433782444No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.18503543085842733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.15899340725613015No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3240.14802834468674186No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.13432201647500652No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2880.13158075083265944No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2820.12883948519031238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC1303.6379788E-1240.253521
GGTATCA13700.037.1550451
TATAGCC1452.3672328E-829.5006032
GTATCAA18800.029.1001051
GTCCTAC856.3337956E-427.9837321
GATAGTC700.00838030327.1406357
GTGCTAG908.8452763E-426.4290811
GTATATG2006.9849193E-1026.164791
TAGCCCT2407.2759576E-1225.7447414
ATATGGG1309.925927E-625.5924053
TACATGG19800.024.0044172
GAGTACT14950.023.98632812-13
CATGGGC3800.023.7643764
TAGAGGT1000.001643572723.7643744
GTAGCCC1000.001643572723.7643743
ACAGCCC1201.7055901E-423.7480538
GTACATG20000.023.5483111
CATGGGG5100.023.2984094
ATCAACG23500.023.05653
TATCAAC23650.022.9102652
Produced by FastQC (version 0.11.5)