##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937596_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 218877 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811629362610052 33.0 31.0 34.0 30.0 34.0 2 31.86668768303659 33.0 31.0 34.0 30.0 34.0 3 31.901844414899692 33.0 31.0 34.0 30.0 34.0 4 35.51167550724836 37.0 35.0 37.0 33.0 37.0 5 35.546786551350756 37.0 35.0 37.0 33.0 37.0 6 35.6344248139366 37.0 35.0 37.0 33.0 37.0 7 35.58358804259927 37.0 35.0 37.0 33.0 37.0 8 35.58669024155119 37.0 35.0 37.0 33.0 37.0 9 37.12655052837895 39.0 37.0 39.0 34.0 39.0 10-11 37.088677659141894 39.0 37.0 39.0 34.0 39.0 12-13 36.98524970645614 39.0 37.0 39.0 33.0 39.0 14-15 38.072773749640206 40.0 38.0 41.0 33.0 41.0 16-17 38.024184816129605 40.0 38.0 41.0 33.0 41.0 18-19 38.02908025968924 40.0 38.0 41.0 33.0 41.0 20-21 38.08416827716023 40.0 38.0 41.0 33.5 41.0 22-23 38.041996189640756 40.0 38.0 41.0 33.5 41.0 24-25 37.95637275730205 40.0 38.0 41.0 33.0 41.0 26-27 37.84737318219822 40.0 37.5 41.0 33.0 41.0 28-29 37.66964779305272 40.0 37.0 41.0 33.0 41.0 30-31 37.378621326132944 39.5 36.5 41.0 33.0 41.0 32-33 37.15663820319175 39.0 36.0 41.0 32.5 41.0 34-35 37.07115640291123 39.5 35.0 41.0 32.0 41.0 36-37 36.8770199701202 39.0 35.0 41.0 31.5 41.0 38-39 36.68784522814183 39.0 35.0 41.0 31.0 41.0 40-41 36.42472712984919 39.0 35.0 41.0 30.5 41.0 42-43 36.10224692407151 39.0 35.0 41.0 30.0 41.0 44-45 35.7491650561731 39.0 35.0 41.0 29.5 41.0 46-47 35.25595654180202 39.0 35.0 40.0 26.0 41.0 48-49 34.825276296732866 38.5 35.0 40.0 24.0 41.0 50-51 33.89788785482257 37.5 34.0 39.5 22.5 40.5 52-53 33.97542683790439 38.0 33.5 39.5 23.0 40.5 54-55 34.22164731790001 38.0 34.5 40.0 21.5 41.0 56-57 33.99674017827364 38.0 34.0 40.0 18.5 41.0 58-59 33.73660320636705 38.0 34.0 40.0 15.5 41.0 60-61 33.401928937257 38.0 33.5 40.0 10.0 41.0 62-63 33.03832289368001 38.0 33.0 40.0 7.5 41.0 64-65 32.61499380930843 37.0 33.0 40.0 2.0 41.0 66-67 32.134671527844404 36.5 32.0 39.5 2.0 41.0 68-69 31.675089205352776 36.0 31.0 39.0 2.0 40.5 70-71 31.208964395528085 35.5 31.0 39.0 2.0 40.0 72-73 30.730337129986246 35.0 30.5 38.0 2.0 40.0 74-75 30.189780104807724 35.0 29.5 37.5 2.0 39.5 76-77 29.633871992032056 35.0 29.0 37.0 2.0 39.0 78-79 29.132750357506726 34.0 29.0 36.5 2.0 39.0 80-81 28.61426508952517 34.0 28.0 36.0 2.0 38.5 82-83 28.082550930431246 34.0 27.0 35.5 2.0 37.0 84-85 27.520331053514074 34.0 26.0 35.0 2.0 37.0 86-87 26.92516116357589 33.0 25.5 35.0 2.0 36.0 88-89 26.37054373004016 33.0 24.5 35.0 2.0 36.0 90-91 25.773719943164426 32.5 21.5 35.0 2.0 35.5 92-93 25.15066224409143 32.0 19.0 35.0 2.0 35.0 94-95 24.400960356730035 31.5 9.5 34.0 2.0 35.0 96-97 23.55678531778122 31.0 2.0 34.0 2.0 35.0 98-99 22.429814462003772 31.0 2.0 34.0 2.0 35.0 100-101 19.46970216148796 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10325962149329726 1101 2 -0.19664180724003089 1101 3 0.10626122968515617 1101 4 -0.01745716192118607 1101 5 -0.11625514692525485 1101 6 -0.3242914797922296 1101 7 -0.11256089079007836 1101 8 -0.1827361617544554 1101 9 0.02972704410694149 1101 10-11 0.17973566585323653 1101 12-13 0.21469682275375135 1101 14-15 0.2258472371621778 1101 16-17 0.3134194578114915 1101 18-19 0.5066953050728173 1101 20-21 0.11826283524184333 1101 22-23 0.21525308910334928 1101 24-25 0.002625081209181701 1101 26-27 0.41453697341673745 1101 28-29 0.40750223541859754 1101 30-31 0.5263182603771384 1101 32-33 0.05149740512010226 1101 34-35 0.329506890124847 1101 36-37 0.738056624724706 1101 38-39 0.4913575239871193 1101 40-41 0.23947871035353074 1101 42-43 0.4928489619767831 1101 44-45 0.8476322386935635 1101 46-47 1.1289446079930556 1101 48-49 1.305512271772038 1101 50-51 1.211136285336515 1101 52-53 1.5421168211968563 1101 54-55 1.5653375168825292 1101 56-57 1.9429171399861787 1101 58-59 1.7558762961415724 1101 60-61 1.7401921539455643 1101 62-63 1.5256258435194283 1101 64-65 1.7550598233811456 1101 66-67 1.6700236860271644 1101 68-69 1.790946283146372 1101 70-71 1.7707513017668681 1101 72-73 1.880296660220555 1101 74-75 1.8698900412886381 1101 76-77 1.9724401517053103 1101 78-79 2.0013439481154904 1101 80-81 2.1274773803006326 1101 82-83 2.0770216966387736 1101 84-85 1.901505338781618 1101 86-87 2.012287617812259 1101 88-89 2.4655749446561366 1101 90-91 2.324773375156358 1101 92-93 2.5833923033004247 1101 94-95 2.7595743348064765 1101 96-97 2.670125114451057 1101 98-99 2.6095816020380056 1101 100-101 3.535291203707171 1102 1 0.37000274075935025 1102 2 0.051490564974912445 1102 3 0.19965354073801578 1102 4 0.335120382858193 1102 5 0.13677019165054105 1102 6 0.03217422575604445 1102 7 0.21548629138554531 1102 8 0.2794416451481254 1102 9 0.28295989688108136 1102 10-11 0.3593501263163219 1102 12-13 0.2718232971967396 1102 14-15 0.39201150103286153 1102 16-17 0.36452802050262534 1102 18-19 0.09526564146425898 1102 20-21 0.4562488553379538 1102 22-23 0.3729256535419623 1102 24-25 0.0554265228867763 1102 26-27 0.27426283623893255 1102 28-29 0.4621330785815516 1102 30-31 0.6180723014431067 1102 32-33 0.6507110355263919 1102 34-35 0.3324339325014378 1102 36-37 0.9738692066381986 1102 38-39 0.9700709359574091 1102 40-41 1.1636049663605235 1102 42-43 1.3395669611030456 1102 44-45 1.574194056081069 1102 46-47 1.358346093357845 1102 48-49 1.2169528571805088 1102 50-51 1.3314508331739958 1102 52-53 1.204922631594414 1102 54-55 1.624664734007915 1102 56-57 1.6309577690818529 1102 58-59 1.6245525739921618 1102 60-61 1.7464994933951061 1102 62-63 2.1701267172634218 1102 64-65 2.0437612213715326 1102 66-67 2.3385033714793195 1102 68-69 2.0550244832337405 1102 70-71 2.1743828002378187 1102 72-73 2.3817492596089274 1102 74-75 2.3016233309347705 1102 76-77 1.9731118423999376 1102 78-79 2.202059326009767 1102 80-81 2.4102318582385998 1102 82-83 2.1629598792512645 1102 84-85 2.141812241359162 1102 86-87 2.6008415715038247 1102 88-89 2.741710811847046 1102 90-91 1.9124426630549998 1102 92-93 1.7285703286390017 1102 94-95 1.4725221286028933 1102 96-97 1.9065984652592753 1102 98-99 1.3859140616273429 1102 100-101 1.7612468611995276 1103 1 -0.08899725924064583 1103 2 -0.20236657788223056 1103 3 -0.3302036021191235 1103 4 -0.3714510457132363 1103 5 -0.30508695120659723 1103 6 -0.368682917101097 1103 7 -0.25079942290017243 1103 8 -0.19298692628044734 1103 9 -0.126468674547489 1103 10-11 -0.0704355879693992 1103 12-13 -0.38524813137468783 1103 14-15 -0.3491313561099858 1103 16-17 -0.39297197949738205 1103 18-19 -0.06023435853574455 1103 20-21 -0.19803685894776635 1103 22-23 -0.27386006074375047 1103 24-25 -0.41278776282751295 1103 26-27 -0.3468085923325006 1103 28-29 -0.2807240642755886 1103 30-31 -0.34864198427118254 1103 32-33 -0.36386039304503726 1103 34-35 -0.12499463892713436 1103 36-37 0.019726349495343243 1103 38-39 -0.23178620689973428 1103 40-41 0.09374782350338506 1103 42-43 -0.45279018175409647 1103 44-45 -0.8649488010617858 1103 46-47 -0.7045110494992954 1103 48-49 -0.8002614285337799 1103 50-51 -0.6931205953974242 1103 52-53 -0.665577368405593 1103 54-55 -0.43333526599209193 1103 56-57 -0.3148993737752903 1103 58-59 -0.9688759974364061 1103 60-61 -1.035000506604895 1103 62-63 -0.3412304255937215 1103 64-65 -0.18831020719989056 1103 66-67 -0.10006805709210553 1103 68-69 0.07431019751945556 1103 70-71 0.19388280023781945 1103 72-73 -0.23325074039106752 1103 74-75 0.04526618807762972 1103 76-77 -0.06581672902863644 1103 78-79 0.17627361172404932 1103 80-81 0.22408900109573793 1103 82-83 0.7656027363941256 1103 84-85 0.7387408127877322 1103 86-87 0.9674130000752541 1103 88-89 0.7484250975613342 1103 90-91 0.7488712344835733 1103 92-93 1.004927471496142 1103 94-95 1.6067364143171794 1103 96-97 1.632241322402134 1103 98-99 1.615771204484485 1103 100-101 2.0407468611995263 1104 1 -0.1552082297047832 1104 2 -0.31362396184847796 1104 3 -0.08276056414444 1104 4 0.07680211884373023 1104 5 -0.01990551660744444 1104 6 -0.0868432546538429 1104 7 -0.024959760452915702 1104 8 0.02436328469001836 1104 9 0.03030769676053069 1104 10-11 -0.30762546138711855 1104 12-13 -0.20068273053080787 1104 14-15 -0.2691397949285559 1104 16-17 -0.3563053128307132 1104 18-19 -0.36079975937962416 1104 20-21 -0.3696402344751917 1104 22-23 -0.36609634766357857 1104 24-25 -0.034981855654514504 1104 26-27 0.046693517372141 1104 28-29 -0.10751731322073255 1104 30-31 0.09048037859801639 1104 32-33 0.06475564071023143 1104 34-35 0.28800958048214653 1104 36-37 0.24721158156285128 1104 38-39 0.0041125272774777955 1104 40-41 0.6210642791995795 1104 42-43 0.5330494806931583 1104 44-45 0.5913128023137375 1104 46-47 0.6108476002897305 1104 48-49 1.0420508077531494 1104 50-51 1.0265080965856939 1104 52-53 0.5546589185142352 1104 54-55 0.6103187424467293 1104 56-57 1.2137335376171166 1104 58-59 1.1029130320994582 1104 60-61 1.2499277634372987 1104 62-63 1.5782716841109163 1104 64-65 1.8203058265553835 1104 66-67 1.8046576813045192 1104 68-69 1.504858720726208 1104 70-71 1.6201106483390824 1104 72-73 1.716242930495003 1104 74-75 1.8187345425080075 1104 76-77 1.939710697131705 1104 78-79 1.7549149619350217 1104 80-81 1.956359043289833 1104 82-83 1.9704719347063637 1104 84-85 2.02626823894807 1104 86-87 2.0121134220161814 1104 88-89 2.3334377557891806 1104 90-91 2.3181539349055136 1104 92-93 3.086353631833699 1104 94-95 2.9937321949078957 1104 96-97 2.4209670607987555 1104 98-99 2.611788082121617 1104 100-101 2.9331350468535327 1105 1 -0.14398031008810364 1105 2 -0.17145132364494486 1105 3 -0.22893806539596184 1105 4 -0.20446799486577305 1105 5 -0.417245143296995 1105 6 -0.36124788885251036 1105 7 -0.4236581799623167 1105 8 -0.2836535929471111 1105 9 -0.2175647197452264 1105 10-11 -0.32689886480555685 1105 12-13 -0.5446718601882452 1105 14-15 -0.36654378548852407 1105 16-17 -0.28285898514709373 1105 18-19 -0.25974848282952934 1105 20-21 -0.5571894013206489 1105 22-23 -0.29114819633696953 1105 24-25 -0.31199680237553906 1105 26-27 4.230460855794149E-4 1105 28-29 -0.23241897952982526 1105 30-31 -0.45196401816948395 1105 32-33 -0.31345361338402 1105 34-35 -0.6096161078536895 1105 36-37 -0.369573085532906 1105 38-39 -0.5630404441878696 1105 40-41 -0.7347606510728966 1105 42-43 -0.8967223851439243 1105 44-45 -0.2676945637736452 1105 46-47 0.1572968601052196 1105 48-49 -0.08281510085016208 1105 50-51 -0.264917205566924 1105 52-53 -0.5654869729253704 1105 54-55 -0.8634708592124269 1105 56-57 -0.7097807297074965 1105 58-59 -0.7191923816171979 1105 60-61 -0.9028649133845548 1105 62-63 -0.7533095216389185 1105 64-65 -0.6059260264089303 1105 66-67 -0.23950308534069364 1105 68-69 -0.5593621188647226 1105 70-71 -0.7725804765983391 1105 72-73 -0.961329836436267 1105 74-75 -0.8996377667246307 1105 76-77 -0.9442913052998172 1105 78-79 -0.5090484221742528 1105 80-81 -0.7572217333675368 1105 82-83 -0.8302503709505089 1105 84-85 -0.6882422380597255 1105 86-87 -0.23103897732587342 1105 88-89 -0.43571049565900566 1105 90-91 -0.506383002804565 1105 92-93 -0.6727109465829528 1105 94-95 -0.8261562410500538 1105 96-97 -0.7926852312701875 1105 98-99 -1.1114830328036476 1105 100-101 -0.2218746077270275 1106 1 -0.27721948146286834 1106 2 -0.1885888001044549 1106 3 0.03735195343643127 1106 4 0.2398822876200981 1106 5 -0.014420284539930606 1106 6 -0.2440162504344343 1106 7 0.08453391043315861 1106 8 0.16634640705288462 1106 9 0.0781979921191791 1106 10-11 -0.07299114352495195 1106 12-13 -0.12758146470802245 1106 14-15 -0.32346468944331974 1106 16-17 -0.19797197949737466 1106 18-19 0.4410989747975904 1106 20-21 0.2641853632744571 1106 22-23 0.346139939256247 1106 24-25 0.05165668161693304 1106 26-27 -0.014030814554722326 1106 28-29 0.6026092690577443 1106 30-31 0.41846912683993764 1106 32-33 0.3165840513994098 1106 34-35 0.22767202773952988 1106 36-37 -0.03049587272687404 1106 38-39 -0.3088973180108425 1106 40-41 -0.03758550982995246 1106 42-43 0.2036542626903426 1106 44-45 -0.22739324550622797 1106 46-47 -0.8483999383881908 1106 48-49 -0.684039206311553 1106 50-51 -0.3832317065085391 1106 52-53 -0.3103551461833689 1106 54-55 -0.4114463771031964 1106 56-57 -0.5896771515530688 1106 58-59 -0.6190982196586319 1106 60-61 -1.0271116177160025 1106 62-63 -1.196341536704832 1106 64-65 -1.8000879849776723 1106 66-67 -1.3678458348698825 1106 68-69 -0.6890231358138763 1106 70-71 -0.6686727553177363 1106 72-73 -1.3099174070577355 1106 74-75 -1.2176227008112583 1106 76-77 -0.8274833956952996 1106 78-79 -0.9386152771648355 1106 80-81 -0.66357766557093 1106 82-83 -0.5590639302725435 1106 84-85 -0.3798147427678238 1106 86-87 -0.4094758888136347 1106 88-89 -0.6614637913275523 1106 90-91 -0.4161287655164294 1106 92-93 -0.6660725285038573 1106 94-95 -0.31259691901615483 1106 96-97 -0.018203122042312003 1106 98-99 -0.19900657329329263 1106 100-101 0.859857972310639 1107 1 -0.0025281234381786533 1107 2 0.08425070606838503 1107 3 0.2299445460290208 1107 4 0.0367958678670135 1107 5 0.35718465373167163 1107 6 0.2652430088248252 1107 7 0.049348725247973846 1107 8 0.22869208606523017 1107 9 0.4170868810080677 1107 10-11 0.43225577005529914 1107 12-13 0.3881592760327166 1107 14-15 0.4774612364826041 1107 16-17 0.3350527118606479 1107 18-19 0.47937057973586406 1107 20-21 0.2314693138917434 1107 22-23 0.3257695688858746 1107 24-25 0.309681372974957 1107 26-27 0.28133955581565573 1107 28-29 -0.06590924946078047 1107 30-31 0.09809875646956101 1107 32-33 0.4178186193006397 1107 34-35 0.6894004228012562 1107 36-37 0.7250596828286717 1107 38-39 0.6886335461866864 1107 40-41 0.9908095518984439 1107 42-43 0.7184690775051621 1107 44-45 0.45594008782710205 1107 46-47 1.0500568517352704 1107 48-49 0.5134916578859787 1107 50-51 0.19176829349145663 1107 52-53 0.5016818908536607 1107 54-55 0.5758993019091463 1107 56-57 0.39766852745928105 1107 58-59 0.31114869392161637 1107 60-61 0.312085913148195 1107 62-63 0.37155969786306997 1107 64-65 0.6610231261334434 1107 66-67 0.7173393503152994 1107 68-69 0.806038592581185 1107 70-71 0.6995371212254753 1107 72-73 0.5092183954113985 1107 74-75 0.4780563115344201 1107 76-77 0.7833190734405022 1107 78-79 1.0471871919709628 1107 80-81 1.5064840628241356 1107 82-83 1.6014298968879537 1107 84-85 1.5334568621704463 1107 86-87 1.2602771976061184 1107 88-89 1.2351411469440485 1107 90-91 0.7576366665823393 1107 92-93 1.463248459150467 1107 94-95 1.4874030809838459 1107 96-97 1.441673421167561 1107 98-99 1.600067500780785 1107 100-101 2.444425873545203 1108 1 0.010792214443561932 1108 2 -0.11636657788223204 1108 3 -0.20109833896123064 1108 4 -0.11581946676586341 1108 5 -0.17977116173291563 1108 6 0.03668550395153147 1108 7 0.341990050784041 1108 8 -0.0709342947015017 1108 9 -0.07267920086327706 1108 10-11 -0.10888295639045253 1108 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-0.7719109989042607 2316 82-83 -1.0646194858281 2316 84-85 -1.3251851131381969 2316 86-87 -1.274290703628452 2316 88-89 -1.5475749024386651 2316 90-91 -1.1781658025534618 2316 92-93 -0.944776232207559 2316 94-95 -0.9737080301272663 2316 96-97 -2.145980899820092 2316 98-99 -2.2758584251451417 2316 100-101 -2.2382901758375127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 938.0 3 174.0 4 132.0 5 133.0 6 200.0 7 220.0 8 251.0 9 343.0 10 445.0 11 546.0 12 872.0 13 1284.0 14 1447.0 15 1916.0 16 2650.0 17 3645.0 18 3070.0 19 2000.0 20 1964.0 21 1990.0 22 2031.0 23 2200.0 24 2490.0 25 2602.0 26 2860.0 27 3370.0 28 3881.0 29 4421.0 30 5418.0 31 6720.0 32 8237.0 33 10638.0 34 14715.0 35 21766.0 36 27935.0 37 39375.0 38 32670.0 39 3327.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.272053970814696 20.06764684314732 14.286499963335045 20.37379922270294 2 14.455656890004533 22.63946974242931 42.18561763372101 20.719255733845145 3 19.167269828763047 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19.51338979171435 30.50194552529183 31.459601739528498 18.525062943465322 24-25 18.860794550710573 30.48081830895329 32.25742216200009 18.400964978336056 26-27 18.930815450643777 30.899456366237484 31.82077253218884 18.3489556509299 28-29 18.41170936277461 31.373913521209946 31.928341043889507 18.28603607212594 30-31 18.66738083956518 31.569417834636926 31.83975058884929 17.923450736948602 32-33 18.280330446844893 31.665533588333183 31.770600851983342 18.283535112838578 34-35 18.54124842910544 31.984672189500817 31.60811531567827 17.86596406571547 36-37 18.49730034080479 31.998571778433398 31.36525622157576 18.13887165918605 38-39 18.476967300157458 32.3940825368926 31.202167783514597 17.926782379435352 40-41 18.708333810117747 32.168072227117214 31.210078841070594 17.913515121694452 42-43 18.11896053721751 32.415922438535375 31.16626003048627 18.298856993760843 44-45 18.289408937460664 32.77908107798822 30.612347656920523 18.3191623276306 46-47 18.27961911737777 32.695019227247755 30.067066471342248 18.958295184032227 48-49 18.531829513120275 32.71266793790904 29.95423985276175 18.801262696208934 50-51 18.687010879392787 33.24360638251606 29.21092929195822 18.858453446132927 52-53 18.47107513688721 33.382853846576445 29.23411833647701 18.911952680059333 54-55 18.513321735945798 32.9971616919978 29.174372825489836 19.315143746566562 56-57 18.77168251759774 33.11807189213434 28.643120634891577 19.46712495537635 58-59 18.727447871262097 32.937491276602074 28.958477769921952 19.376583082213877 60-61 18.867255260038355 32.63100321758274 29.263525976373884 19.238215546005026 62-63 19.080722260232438 32.34473716125459 29.456748822128205 19.117791756384765 64-65 18.99572512655273 32.52611151286581 29.340095017529727 19.138068343051728 66-67 18.86542448749811 32.614983957855614 29.239954779918254 19.279636774728015 68-69 18.986921747886083 32.28325915077176 29.46897490760552 19.260844193736627 70-71 18.95580693367264 32.06314406742813 29.418105174508945 19.56294382439029 72-73 18.8586704018273 31.877261523039213 29.4244602669999 19.839607808133586 74-75 18.839491705423516 32.00278316915077 29.442542205295346 19.71518292013037 76-77 18.766235698280934 31.66759360344958 29.834318632802574 19.731852065466914 78-79 18.49380122259069 31.790333248653706 29.67402921675924 20.041836311996356 80-81 18.30315763455478 32.069547147097666 29.48910722500292 20.13818799334464 82-83 18.522188455412554 31.89544697288688 29.517785548212295 20.064579023488275 84-85 18.719450800915332 31.570022883295195 29.62562929061785 20.084897025171625 86-87 18.58963796970113 31.560254473888964 29.552611103482995 20.297496452926907 88-89 18.862142517118897 31.22825808341572 29.631257094730675 20.278342304734704 90-91 18.664594780769907 31.557049713818852 29.655964719963567 20.12239078544767 92-93 18.459595034967595 31.719819852806562 29.51512211196954 20.305463000256307 94-95 18.4982527444613 31.895251629970865 28.989566813358326 20.616928812209505 96-97 18.76687874765414 31.666590378541677 28.86872339451641 20.697807479287775 98-99 19.393478902668722 31.388187691526053 28.600918638474337 20.617414767330892 100-101 19.674884557474133 31.001295153015697 28.766584136892643 20.557236152617538 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1076.0 1 666.0 2 454.5 3 627.0 4 551.0 5 531.0 6 750.5 7 1200.5 8 1534.5 9 1457.0 10 1155.0 11 928.5 12 879.0 13 880.5 14 837.0 15 730.5 16 643.0 17 613.5 18 628.5 19 663.5 20 715.0 21 809.0 22 946.0 23 1137.0 24 1355.5 25 1667.5 26 2022.0 27 2439.0 28 2841.5 29 3353.0 30 3969.5 31 4540.5 32 5226.5 33 5950.0 34 6644.5 35 7278.5 36 7801.5 37 8186.5 38 8388.0 39 8448.0 40 8355.5 41 8337.0 42 8279.5 43 8101.0 44 8007.5 45 7861.5 46 7648.0 47 7435.5 48 7741.5 49 7363.5 50 6370.0 51 5717.5 52 4872.0 53 4201.5 54 3685.0 55 3199.0 56 2726.0 57 2222.0 58 1728.0 59 1329.0 60 1047.5 61 793.5 62 558.0 63 371.0 64 243.5 65 178.0 66 128.0 67 92.0 68 67.0 69 44.5 70 33.0 71 30.5 72 24.0 73 19.5 74 17.5 75 15.5 76 10.5 77 6.0 78 5.5 79 6.5 80 4.5 81 1.5 82 1.5 83 2.5 84 1.0 85 1.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.312961160834624 2 0.25950648080885613 3 0.2663596449147238 4 0.2604202360229718 5 0.2681871553429552 6 0.20468116796191468 7 0.19600049342781561 8 0.20422429035485684 9 0.20285365753368328 10-11 0.20719399480073286 12-13 0.20970682163955096 14-15 0.20559492317603037 16-17 0.19462986060664209 18-19 0.1886904517148901 20-21 0.18709138009018764 22-23 0.19508673821369993 24-25 0.19485829941017102 26-27 0.200797708301923 28-29 0.20719399480073286 30-31 0.20216834112309653 32-33 0.20353897394427006 34-35 0.2058233619795593 36-37 0.19348766658899746 38-39 0.18503543085842733 40-41 0.18252260401960918 42-43 0.19097483975017931 44-45 0.18937576812547685 46-47 0.19965551428427838 48-49 0.20810775001484852 50-51 0.19942707548074945 52-53 0.20513804556897253 54-55 0.19965551428427838 56-57 0.17498412350315473 58-59 0.16241998930906398 60-61 0.17818226675255966 62-63 0.168359398200816 64-65 0.1786391443596175 66-67 0.17818226675255966 68-69 0.1756694399137415 70-71 0.17932446077020425 72-73 0.18846201291136117 74-75 0.19234547257135284 76-77 0.18983264573253472 78-79 0.1857207472690141 80-81 0.1857207472690141 82-83 0.17589787871727042 84-85 0.17224285786080767 86-87 0.17681163393138613 88-89 0.18503543085842733 90-91 0.18115197119843565 92-93 0.17772538914550182 94-95 0.1788675831631464 96-97 0.18594918607254302 98-99 0.18343635923372487 100-101 0.16904471461140275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 218877.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.78318639872042 #Duplication Level Percentage of deduplicated Percentage of total 1 93.41168026823465 79.19739899999412 2 5.2392121840201735 8.883942063604588 3 0.7135621766678285 1.814942250945155 4 0.20939004427889202 0.7101102061653448 5 0.10173422253532323 0.4312675776170605 6 0.06000524551883117 0.30524615494344276 7 0.04173564716752138 0.24769368072926345 8 0.029720194716893182 0.20158182467908958 9 0.02266224024871281 0.1729239245297282 >10 0.14604949576208942 2.373548869053954 >50 0.014010117823912666 0.8182269130002636 >100 0.007543909597491435 1.4176203858402112 >500 5.388506855351025E-4 0.27045803042778116 >1k 0.00215540274214041 3.155039118470028 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2285 1.0439653321271765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1744 0.7967945467088822 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1622 0.7410554786478251 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.5733813968575958 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.2704715433782444 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 405 0.18503543085842733 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 348 0.15899340725613015 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 324 0.14802834468674186 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.13432201647500652 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 288 0.13158075083265944 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 282 0.12883948519031238 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.137552141156906E-4 0.0 24-25 0.0 0.0 0.0 0.0018275104282313812 0.0 26-27 0.0 0.0 0.0 0.003655020856462762 0.0 28-29 0.0 0.0 0.0 0.0050256536776362975 0.0 30-31 0.0 0.0 0.0 0.009137552141156906 0.0 32-33 0.0 0.0 0.0 0.014391644622322126 0.0 34-35 0.0 0.0 0.0 0.019645737103487347 0.0 36-37 4.568776070578453E-4 0.0 0.0 0.023757635567007954 0.0 38-39 4.568776070578453E-4 0.0 0.0 0.031752993690520245 0.0 40-41 4.568776070578453E-4 0.0 0.0 0.04865746515166052 0.0 42-43 4.568776070578453E-4 0.0 0.0 0.08086733644923862 0.0 44-45 4.568776070578453E-4 0.0 0.0 0.1059956048374201 0.0 46-47 4.568776070578453E-4 0.0 0.0 0.1715575414502209 0.0 48-49 4.568776070578453E-4 0.0 0.0 0.23552040643831923 0.0 50-51 4.568776070578453E-4 0.0 0.0 0.27412656423470716 0.0 52-53 4.568776070578453E-4 0.0 0.0 0.33671879640163194 0.0 54-55 4.568776070578453E-4 0.0 0.0 0.4034229270320774 0.0 56-57 4.568776070578453E-4 0.0 0.0 0.5413999643635466 0.0 58-59 4.568776070578453E-4 0.0 0.0 0.6750366644279664 0.0 60-61 4.568776070578453E-4 0.0 0.0 0.8136990181700224 0.0 62-63 4.568776070578453E-4 0.0 0.0 0.9619558016602932 0.0 64-65 4.568776070578453E-4 0.0 0.0 1.0949071853141263 0.0 66-67 4.568776070578453E-4 0.0 0.0 1.3011874249007434 0.0 68-69 4.568776070578453E-4 0.0 0.0 1.6162045349671277 0.0 70-71 4.568776070578453E-4 0.0 0.0 1.8259113566066785 0.0 72-73 4.568776070578453E-4 0.0 0.0 2.043613536369742 0.0 74-75 4.568776070578453E-4 0.0 0.0 2.308830987266821 0.0 76-77 4.568776070578453E-4 0.0 0.0 2.6158527392096933 0.0 78-79 4.568776070578453E-4 0.0 0.0 2.9324689209007797 0.0 80-81 4.568776070578453E-4 0.0 0.0 3.4053372442056498 0.0 82-83 4.568776070578453E-4 0.0 0.0 3.9122429492363286 0.0 84-85 4.568776070578453E-4 0.0 0.0 4.478771181988057 0.0 86-87 4.568776070578453E-4 0.0 0.0 4.949811994864696 0.0 88-89 4.568776070578453E-4 0.0 0.0 5.660485112643174 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGC 130 3.6379788E-12 40.25352 1 GGTATCA 1370 0.0 37.155045 1 TATAGCC 145 2.3672328E-8 29.500603 2 GTATCAA 1880 0.0 29.100105 1 GTCCTAC 85 6.3337956E-4 27.983732 1 GATAGTC 70 0.008380303 27.140635 7 GTGCTAG 90 8.8452763E-4 26.429081 1 GTATATG 200 6.9849193E-10 26.16479 1 TAGCCCT 240 7.2759576E-12 25.744741 4 ATATGGG 130 9.925927E-6 25.592405 3 TACATGG 1980 0.0 24.004417 2 GAGTACT 1495 0.0 23.986328 12-13 CATGGGC 380 0.0 23.764376 4 TAGAGGT 100 0.0016435727 23.764374 4 GTAGCCC 100 0.0016435727 23.764374 3 ACAGCCC 120 1.7055901E-4 23.748053 8 GTACATG 2000 0.0 23.548311 1 CATGGGG 510 0.0 23.298409 4 ATCAACG 2350 0.0 23.0565 3 TATCAAC 2365 0.0 22.910265 2 >>END_MODULE