FastQCFastQC Report
Thu 26 May 2016
SRR937596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218877
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36481.6666895105470196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31051.4186049699146097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.9964500609931605No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.6958245955490984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10330.47195456809075415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.31478867126285537No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.29925483262288866No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.28920352526761606No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.15716589682789878No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.14757146707968402No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACCG250.005012564457.009465
GGTATCA14050.039.927231
TAGCCCT2100.036.1964844
GTATCAA21900.032.779051
CCTAGGA1051.8899336E-631.6719232
GTATTAA1056.8409325E-527.1659851
GTATAGC1754.8694346E-927.1659831
TAGGACT700.008370209527.1473624
TATCAAC27100.026.646492
ATAGCCC1806.6229404E-926.393273
AGTACAG908.9156744E-426.393275
GGAGTGT908.927583E-426.3872416
ATCAACG27650.026.1164513
ACAGTAT1109.446315E-525.9074736
TCAACGC27900.025.8824314
CAACGCA28450.025.3820695
GTACAGA950.001217320425.0213011
GTGTAGC1901.1692464E-825.0213011
GTCTTAC950.001217320425.0213011
TAGACTG950.001222199525.0041485
Produced by FastQC (version 0.11.5)