##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 218877 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.209656565102776 33.0 31.0 34.0 31.0 34.0 2 32.370957204274546 34.0 31.0 34.0 31.0 34.0 3 32.46925442143304 34.0 31.0 34.0 31.0 34.0 4 36.11152839265889 37.0 35.0 37.0 35.0 37.0 5 35.98747241601448 37.0 35.0 37.0 35.0 37.0 6 36.09626411180709 37.0 35.0 37.0 35.0 37.0 7 36.056570585305906 37.0 35.0 37.0 35.0 37.0 8 36.067965112825924 37.0 35.0 37.0 35.0 37.0 9 37.7008867994353 39.0 37.0 39.0 35.0 39.0 10-11 37.67911429707095 39.0 37.0 39.0 35.0 39.0 12-13 37.58945435107389 39.0 37.0 39.0 35.0 39.0 14-15 38.74624103948793 40.0 38.0 41.0 34.5 41.0 16-17 38.733784271531505 40.0 38.0 41.0 34.5 41.0 18-19 38.81053742512918 40.0 38.0 41.0 35.0 41.0 20-21 38.79228744911526 40.0 38.0 41.0 35.0 41.0 22-23 38.627356917355414 40.0 38.0 41.0 34.0 41.0 24-25 38.54928110308529 40.0 38.0 41.0 34.0 41.0 26-27 38.42814914312605 40.0 38.0 41.0 34.0 41.0 28-29 38.282560524861 40.0 38.0 41.0 34.0 41.0 30-31 38.17209665702654 40.0 37.0 41.0 34.0 41.0 32-33 37.92064949720619 40.0 36.5 41.0 33.5 41.0 34-35 37.7342114520941 40.0 36.0 41.0 33.0 41.0 36-37 37.529036856316566 40.0 35.5 41.0 33.0 41.0 38-39 37.363263385371695 40.0 35.0 41.0 33.0 41.0 40-41 37.12400800449568 40.0 35.0 41.0 33.0 41.0 42-43 36.83575021587467 40.0 35.0 41.0 32.5 41.0 44-45 36.376592789557606 39.5 35.0 41.0 31.0 41.0 46-47 35.80196640122078 39.0 35.0 41.0 29.0 41.0 48-49 35.31901707351618 39.0 35.0 41.0 26.0 41.0 50-51 35.03033667310864 39.0 35.0 41.0 24.0 41.0 52-53 34.79902867820739 39.0 35.0 40.5 23.5 41.0 54-55 34.590251602498206 39.0 35.0 40.0 23.0 41.0 56-57 34.34860675173728 38.5 35.0 40.0 21.5 41.0 58-59 34.12320846868333 38.0 34.5 40.0 19.5 41.0 60-61 33.820472228694655 38.0 34.0 40.0 17.5 41.0 62-63 33.471333214545155 38.0 34.0 40.0 11.5 41.0 64-65 33.09511734901337 37.5 33.0 40.0 7.5 41.0 66-67 32.69203251141052 37.0 33.0 40.0 3.5 41.0 68-69 32.257528200770295 36.0 32.5 39.0 2.0 40.5 70-71 31.864243387838833 36.0 32.0 39.0 2.0 40.0 72-73 31.42234679751641 35.0 31.0 38.5 2.0 40.0 74-75 30.930643694860585 35.0 31.0 38.0 2.0 40.0 76-77 29.629511095272687 34.0 29.5 36.0 2.0 39.0 78-79 30.137661791782598 35.0 30.5 37.0 2.0 39.0 80-81 29.907045966456046 35.0 30.5 36.0 2.0 39.0 82-83 29.512769729117267 34.0 30.0 36.0 2.0 37.5 84-85 29.06698282597075 34.0 29.5 35.5 2.0 37.0 86-87 28.60991333031794 34.0 29.0 35.0 2.0 37.0 88-89 28.150584574898232 34.0 29.0 35.0 2.0 36.0 90-91 27.610121209629153 34.0 27.0 35.0 2.0 36.0 92-93 27.19914152697634 34.0 26.5 35.0 2.0 35.5 94-95 26.745918940774956 33.0 25.5 35.0 2.0 35.0 96-97 26.2328134066165 33.0 25.0 35.0 2.0 35.0 98-99 25.49597033950575 33.0 22.0 35.0 2.0 35.0 100-101 23.27317169003596 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09909609166613365 1101 2 0.03145469934620593 1101 3 0.02476087341958788 1101 4 0.0440286292341554 1101 5 -0.11176631538586435 1101 6 -0.10225874579335681 1101 7 0.023673844049092452 1101 8 -0.015591237713834971 1101 9 0.08030203818555037 1101 10-11 0.13991092572593544 1101 12-13 0.06700634265970962 1101 14-15 0.039490608168186725 1101 16-17 -0.1029195876631448 1101 18-19 0.23510183923176697 1101 20-21 -0.04150350692034266 1101 22-23 0.09096225015216675 1101 24-25 -0.2000683714322804 1101 26-27 -0.293950825347423 1101 28-29 0.03857775274173747 1101 30-31 0.1577484614861575 1101 32-33 0.3815894429713822 1101 34-35 0.0914273772389862 1101 36-37 0.14629822398934067 1101 38-39 0.07684436074521983 1101 40-41 -0.1207505571183134 1101 42-43 -0.13852138410910442 1101 44-45 0.2599388790781916 1101 46-47 0.2658523873328136 1101 48-49 0.4065627811468886 1101 50-51 0.5799495502580072 1101 52-53 0.6997800404422279 1101 54-55 0.6663060161747865 1101 56-57 0.6835569867811557 1101 58-59 0.5610278778908437 1101 60-61 0.7866025105868246 1101 62-63 0.7061324103810307 1101 64-65 0.7780651263194187 1101 66-67 0.917148644202257 1101 68-69 1.0060161878789344 1101 70-71 0.7991987932802083 1101 72-73 0.6917546579613543 1101 74-75 0.8369073143675791 1101 76-77 0.4370230823994099 1101 78-79 0.8287288803893098 1101 80-81 0.9772765505571286 1101 82-83 0.766800459291666 1101 84-85 0.8645137494725965 1101 86-87 1.19201017588777 1101 88-89 1.0915690764757215 1101 90-91 1.0813526889245253 1101 92-93 1.197066324136216 1101 94-95 1.8338544515983308 1101 96-97 2.0329745451430945 1101 98-99 1.325143189427159 1101 100-101 1.4470407539746724 1102 1 0.057571408398331414 1102 2 0.13032975395520907 1102 3 -0.10687958092728422 1102 4 0.050319585983828574 1102 5 -0.10218789686248897 1102 6 -0.19971396641371086 1102 7 0.10742220577910899 1102 8 -0.1410390315102532 1102 9 0.04133305609730087 1102 10-11 -0.03933765444006809 1102 12-13 -0.029504142268208966 1102 14-15 -0.11378593180560159 1102 16-17 -0.1976662019051716 1102 18-19 0.12624972040257632 1102 20-21 0.2572186424898746 1102 22-23 0.4029653082561424 1102 24-25 0.08526911218501709 1102 26-27 0.2857892358146543 1102 28-29 0.047320653571794935 1102 30-31 0.39679389617379 1102 32-33 0.24842539753669968 1102 34-35 0.28840749956314227 1102 36-37 0.08773553285784175 1102 38-39 0.24405711740752878 1102 40-41 0.42535582121283255 1102 42-43 0.6322245748249244 1102 44-45 0.9680537764132708 1102 46-47 0.8641813519461863 1102 48-49 0.7341512477261745 1102 50-51 0.8857435651116532 1102 52-53 0.49545500767682427 1102 54-55 0.4479901577213141 1102 56-57 0.6690965236968367 1102 58-59 0.8395993064622687 1102 60-61 0.9268056560651985 1102 62-63 0.6486892037405809 1102 64-65 0.9391343705308657 1102 66-67 1.213189491733928 1102 68-69 1.5782802770008217 1102 70-71 1.2978499509909973 1102 72-73 1.4643354792807095 1102 74-75 1.4111700928734763 1102 76-77 1.240163099874291 1102 78-79 1.4894595488034632 1102 80-81 1.4769161311600136 1102 82-83 1.6076589127647978 1102 84-85 1.6407369910628127 1102 86-87 1.307420835564482 1102 88-89 1.4076951140467138 1102 90-91 1.2688636107244378 1102 92-93 1.6080318112484946 1102 94-95 1.2666580776359027 1102 96-97 1.4656744140814943 1102 98-99 1.5426289910872732 1102 100-101 2.227796542528626 1103 1 -0.12271430588737786 1103 2 0.08190118252663581 1103 3 0.10440613335843096 1103 4 -0.2931089854447393 1103 5 -0.010616468291061665 1103 6 -0.30014253784228373 1103 7 -0.005720651363752438 1103 8 -0.05675331722453336 1103 9 0.049904484668729765 1103 10-11 0.1555194884170774 1103 12-13 0.053281572017510825 1103 14-15 0.12649978248010996 1103 16-17 0.1307623695234028 1103 18-19 -0.12582170816884997 1103 20-21 -0.33120992893869783 1103 22-23 -0.10803469174386038 1103 24-25 0.1631976836135891 1103 26-27 0.012789235814651079 1103 28-29 0.010534939286081624 1103 30-31 0.0012224676023677716 1103 32-33 0.2703539689652743 1103 34-35 0.2149074995631466 1103 36-37 0.02773553285784658 1103 38-39 0.31034283169324084 1103 40-41 0.10099867835568688 1103 42-43 0.5684388605392101 1103 44-45 0.7733394906989801 1103 46-47 1.0854670662318995 1103 48-49 0.29786553344045785 1103 50-51 0.365814993683081 1103 52-53 0.3763121505339697 1103 54-55 0.31184730057845655 1103 56-57 0.7148108094111194 1103 58-59 0.48502787789085033 1103 60-61 0.2915199417794838 1103 62-63 0.4644749180262906 1103 64-65 1.0522057991022962 1103 66-67 1.3231180631624966 1103 68-69 1.2472088484293948 1103 70-71 1.457064236705282 1103 72-73 1.6045497649949958 1103 74-75 1.3853843785877658 1103 76-77 1.5418059570171465 1103 78-79 1.4946738345177515 1103 80-81 1.2384875597314426 1103 82-83 0.9055160556219377 1103 84-85 0.9217369910628115 1103 86-87 0.8475636927073431 1103 88-89 1.2140522569038588 1103 90-91 1.0046493250101491 1103 92-93 1.2608889541056385 1103 94-95 1.3003009347787575 1103 96-97 1.4951029855100657 1103 98-99 1.6190575625158452 1103 100-101 1.7003679711000537 1104 1 -0.004182660317759712 1104 2 0.005209199404269782 1104 3 0.13156225150189016 1104 4 -0.002965525529127433 1104 5 -0.2813168902319916 1104 6 -0.2817965462811003 1104 7 -0.3661763475662809 1104 8 -0.36367314844816434 1104 9 -0.038112392968400854 1104 10-11 -0.02981384491625505 1104 12-13 -0.18288720435380412 1104 14-15 -0.0722850276464655 1104 16-17 -0.10355830558209078 1104 18-19 0.06102217368769658 1104 20-21 0.06311074616679235 1104 22-23 0.01150961205360801 1104 24-25 -0.34451539655518815 1104 26-27 -0.14385211439631718 1104 28-29 -0.08115282442699367 1104 30-31 -0.0666931442119818 1104 32-33 -0.08072619981110307 1104 34-35 -0.09360726836934674 1104 36-37 -0.3736568722054514 1104 38-39 -0.5785769995303909 1104 40-41 -0.4452966802941063 1104 42-43 -0.22589025338483992 1104 44-45 -0.3719854038157848 1104 46-47 0.1376442814217711 1104 48-49 0.13779802289194265 1104 50-51 0.18135085866198608 1104 52-53 0.020590631546625104 1104 54-55 0.12217641450251193 1104 56-57 0.3864563790313724 1104 58-59 0.14119665426215278 1104 60-61 0.21241445654742108 1104 62-63 0.4002639475621592 1104 64-65 0.29202014509385776 1104 66-67 0.5661054049346532 1104 68-69 0.637630789357658 1104 70-71 0.5588279497854565 1104 72-73 0.6205666426321237 1104 74-75 0.8304814250012669 1104 76-77 0.7026329612365565 1104 78-79 0.8854586446443342 1104 80-81 0.8621331293516938 1104 82-83 1.1959801906430343 1104 84-85 1.4167918433834856 1104 86-87 1.1887198108508024 1104 88-89 1.0211746197730562 1104 90-91 0.817484768048125 1104 92-93 1.1449817811098555 1104 94-95 1.4447397533441624 1104 96-97 2.0778202850881264 1104 98-99 2.2755554528112008 1104 100-101 2.8000388571760055 1105 1 -0.059821650520149205 1105 2 0.006782538458836029 1105 3 -0.07776335816699742 1105 4 0.13877802020497398 1105 5 -0.27545262648315116 1105 6 -0.1413402779552797 1105 7 -0.1514155666179846 1105 8 -0.15584936242227343 1105 9 0.3151022247817252 1105 10-11 -0.2162545228823589 1105 12-13 0.06394823868417632 1105 14-15 -0.1732290310792095 1105 16-17 -0.2286387604201039 1105 18-19 -0.4302171883948418 1105 20-21 -0.16209128487090396 1105 22-23 -0.15508553920149382 1105 24-25 -0.04407915254459738 1105 26-27 0.0607892358146529 1105 28-29 0.22335979804314832 1105 30-31 0.18418291957976152 1105 32-33 -0.30832399713642644 1105 34-35 -0.27253882812046726 1105 36-37 -0.28429836544724196 1105 38-39 0.02472701248419895 1105 40-41 -0.0832499092149348 1105 42-43 -0.372103512342143 1105 44-45 -0.05706728896203117 1105 46-47 0.2057269532375443 1105 48-49 0.19189943174554713 1105 50-51 0.14899013492601654 1105 52-53 -0.027789544381285225 1105 54-55 0.20906763956151053 1105 56-57 0.39153397325292616 1105 58-59 0.6570730756309544 1105 60-61 0.4666159869772315 1105 62-63 0.696802601642112 1105 64-65 1.0502622962774382 1105 66-67 0.9623723004506388 1105 68-69 1.0417286224406936 1105 70-71 0.3315162141064114 1105 72-73 0.5809113469158973 1105 74-75 0.0862205367798552 1105 76-77 0.3809923976951133 1105 78-79 0.42708061417876664 1105 80-81 0.47455535634161095 1105 82-83 0.3865330047744777 1105 84-85 0.5781663695938839 1105 86-87 0.5669648226508421 1105 88-89 0.5295776806326735 1105 90-91 0.35313520071636617 1105 92-93 0.7700979936536569 1105 94-95 0.6481653415584212 1105 96-97 0.4786284092388797 1105 98-99 0.4630688619508767 1105 100-101 1.1340515869192664 1106 1 -0.16782541699848963 1106 2 0.10056784919330397 1106 3 -0.32314942219712606 1106 4 -0.1099978743336294 1106 5 -0.14306091273550692 1106 6 -0.27392031562006025 1106 7 -0.29972065136374937 1106 8 -0.2211977616689822 1106 9 -0.23009551533127137 1106 10-11 -0.14925828936070928 1106 12-13 -0.48882953909360793 1106 14-15 -0.2522779952976677 1106 16-17 -0.2095709638099308 1106 18-19 -0.020377263724405736 1106 20-21 0.09412340439463662 1106 22-23 -0.06925691396608613 1106 24-25 -0.31413564971974495 1106 26-27 -0.5419885419631214 1106 28-29 -0.310020616269469 1106 30-31 -0.7595553101754149 1106 32-33 -0.8009793643680609 1106 34-35 -0.5490925004368563 1106 36-37 -0.3178200226977097 1106 38-39 -0.2527682794178716 1106 40-41 -0.2050013216443105 1106 42-43 -0.5306722505719037 1106 44-45 -0.5232160648565696 1106 46-47 -0.5326440448792127 1106 48-49 -0.8106900221150966 1106 50-51 -1.253740561872469 1106 52-53 -0.983910071688257 1106 54-55 -0.5897082549770971 1106 56-57 -0.6368558572555472 1106 58-59 -0.958416566553602 1106 60-61 -1.1235911693316183 1106 62-63 -1.068969526418151 1106 64-65 -1.1086830897865916 1106 66-67 -1.5306597146152754 1106 68-69 -1.9822355960150517 1106 70-71 -1.5178246521836058 1106 72-73 -1.5985613461161172 1106 74-75 -1.7045045103011205 1106 76-77 -1.8318607096495185 1106 78-79 -1.7401039432600278 1106 80-81 -1.7222902180463358 1106 82-83 -1.6550394999336149 1106 84-85 -1.8564852311594109 1106 86-87 -1.635436307292661 1106 88-89 -1.348058854207256 1106 90-91 -1.5015728972120712 1106 92-93 -1.6435554903388088 1106 94-95 -1.0664768429990161 1106 96-97 -1.0490081256010448 1106 98-99 -0.42760910415082165 1106 100-101 0.4452568599889446 1107 1 0.021680755841011035 1107 2 -0.22412350883138998 1107 3 -0.010803743184780501 1107 4 -0.26308429408671685 1107 5 -0.368986838661435 1107 6 -0.2998462415459855 1107 7 -0.19169596000572398 1107 8 -0.22490146537268174 1107 9 -0.11281156471398646 1107 10-11 -0.11623359800267963 1107 12-13 -0.016298674896077614 1107 14-15 -0.19394466196433058 1107 16-17 -0.1432129391185768 1107 18-19 0.024375822695347438 1107 20-21 0.0749876019254998 1107 22-23 0.0038912341820633856 1107 24-25 0.13524706632964012 1107 26-27 0.020665779024525932 1107 28-29 0.134115186199665 1107 30-31 -0.2055429644964022 1107 32-33 -0.14851022856559126 1107 34-35 -0.06020361154796916 1107 36-37 -0.258560763438453 1107 38-39 -0.273138649788244 1107 40-41 -0.47166798831098333 1107 42-43 0.07982157658859279 1107 44-45 -0.33772223769607734 1107 46-47 -0.6798662671014384 1107 48-49 -0.9727270591521346 1107 50-51 -0.7154689569342025 1107 52-53 -0.6789718000833176 1107 54-55 -0.5415601068289462 1107 56-57 -0.42605338811974036 1107 58-59 -0.926626443096815 1107 60-61 -0.7235911693316268 1107 62-63 -0.6970559461712398 1107 64-65 0.004279873176372462 1107 66-67 -0.39732638128194253 1107 68-69 -0.4455072009533225 1107 70-71 0.043903742878125485 1107 72-73 -0.11954900043710381 1107 74-75 -0.41592426338754507 1107 76-77 -0.48278663557544377 1107 78-79 -0.6533755481983015 1107 80-81 -0.5707470081697927 1107 82-83 -0.22201480857559375 1107 84-85 -0.3898185644927459 1107 86-87 -0.8240165542062421 1107 88-89 -0.32305885420725744 1107 90-91 -0.01268400832318406 1107 92-93 -0.35158018169683203 1107 94-95 -0.048266966455809523 1107 96-97 0.15068323242364556 1107 98-99 0.2754773156022665 1107 100-101 0.9514296994951188 1108 1 -0.14750377957159344 1108 2 0.02205907726347789 1108 3 0.06530087020053088 1108 4 0.02362785666051792 1108 5 -0.01177436302790369 1108 6 0.06175219899981954 1108 7 0.0950161907415108 1108 8 -0.08991121196137897 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-0.37045360359064006 2316 60-61 -0.24951709525754495 2316 62-63 -0.3606361930848223 2316 64-65 -0.1364608675643666 2316 66-67 -0.18436341831898062 2316 68-69 -0.1387170774965334 2316 70-71 -0.32523205959101276 2316 72-73 -0.31893171648648533 2316 74-75 -0.24431932511593857 2316 76-77 -0.6513051540939649 2316 78-79 -0.628066906222994 2316 80-81 -0.1528457736018929 2316 82-83 -0.14855801845213534 2316 84-85 -0.4527815274557092 2316 86-87 -0.3159918628482181 2316 88-89 -0.23046626161466222 2316 90-91 -0.2386099342491086 2316 92-93 -0.5398517866351042 2316 94-95 -0.33221758373975874 2316 96-97 -0.23697108856400817 2316 98-99 -0.48501651155822856 2316 100-101 -1.7010394363073509 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 4.0 5 10.0 6 19.0 7 40.0 8 65.0 9 135.0 10 254.0 11 393.0 12 743.0 13 1058.0 14 1273.0 15 1941.0 16 2834.0 17 4070.0 18 3628.0 19 1967.0 20 1539.0 21 1408.0 22 1496.0 23 1666.0 24 1819.0 25 1958.0 26 2195.0 27 2597.0 28 3073.0 29 3678.0 30 4424.0 31 5456.0 32 6747.0 33 8986.0 34 12431.0 35 19553.0 36 25536.0 37 43737.0 38 46662.0 39 5479.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.696606102159755 19.641640955319392 15.742200891326705 19.91955205119415 2 14.733845950008453 21.54132229517035 42.983502149609144 20.741329605212062 3 19.098397730232048 27.915221791234345 30.844264130082195 22.142116348451413 4 10.977398264778849 18.614564344357788 39.43402002037674 30.974017370486624 5 11.537530100387935 38.94064911743607 35.08277321099744 14.439047571178564 6 27.483472452564683 38.105419939052524 19.485373063410044 14.925734544972746 7 25.279951753724696 35.357758010206645 21.98860547247998 17.37368476358868 8 24.82947043316566 35.506243232500445 21.04469633629847 18.619589998035426 9 26.34264906774124 16.79299332501816 22.220242419258305 34.644115187982294 10-11 23.377056520328768 28.19597308077139 28.19642995837845 20.230540440521388 12-13 25.459185072144603 26.390348432374143 29.12946734531631 19.02099915016494 14-15 21.71447890824527 27.121853826578395 29.78590715333269 21.37776011184364 16-17 18.620503753249544 30.20463548020121 30.77710312184469 20.39775764470456 18-19 18.175961841582257 27.01562978293745 36.072086148841585 18.736322226638706 20-21 19.84932142098398 26.04345372847245 36.38079008025073 17.726434770292837 22-23 18.69044872336683 26.98736502091362 36.03721733488977 18.284968920829783 24-25 18.234650450372243 26.790238241549382 36.766503178762264 18.208608129316115 26-27 18.377533747124442 27.07834084538574 36.31668041147619 18.227444996013624 28-29 17.98610127519201 27.25785743983844 36.713285115754175 18.042756169215377 30-31 18.537292975442604 27.224443175328382 36.606510565391204 17.631753283837806 32-33 17.810916633543037 27.340698200359103 36.6377006263792 18.210684539718656 34-35 18.133700662929407 27.68541235488425 36.27288385714351 17.908003125042832 36-37 17.9854849652658 27.68890218913406 36.11260231226285 18.213010533337293 38-39 18.0823019321354 27.808540869986338 36.19567154155073 17.913485656327527 40-41 18.392978704934734 27.5412674698575 36.05472479977339 18.01102902543438 42-43 17.827704061117814 27.941431809043387 36.00906532148993 18.22179880834887 44-45 18.219136775449225 28.239604892245417 35.38996788150423 18.151290450801135 46-47 18.049635183230762 28.20533907171608 34.5264692041649 19.21855654088826 48-49 18.18441407731283 28.39791298309096 34.22058964623967 19.197083293356542 50-51 18.44460587453227 28.94228265190038 33.45828935886365 19.15482211470369 52-53 18.38384115279358 28.950278010023894 33.32145451554983 19.3444263216327 54-55 18.40896942118176 28.613559213622263 33.105579846215 19.87189151898098 56-57 18.774928384435093 28.870095989985245 32.579942159294944 19.775033466284718 58-59 18.61502122196485 28.762958191130178 32.93790576442477 19.684114822480208 60-61 18.887548714574855 28.453423612348487 33.09941199851972 19.559615674556944 62-63 18.844373780707883 28.26450472183007 33.28924464425225 19.60187685320979 64-65 19.000169044714614 28.23092421771132 33.201752582500674 19.567154155073396 66-67 18.779725599309202 28.274556029185344 33.30957569776633 19.636142673739133 68-69 19.15071021624017 28.21995915514193 33.28033553091462 19.348995097703277 70-71 18.761678933830417 27.943319764068402 33.57959036353751 19.715410938563668 72-73 18.429072035892304 27.830699433928647 33.39615400430379 20.344074525875264 74-75 18.581897595453153 27.874102806599137 33.13710440110199 20.406895196845717 76-77 18.324903941483118 27.86633588727916 33.661371455200864 20.14738871603686 78-79 18.135528173357642 27.580558944064475 33.894379034800366 20.389533847777518 80-81 18.194465384668103 28.00979545589532 33.419683201067265 20.376055958369314 82-83 17.977448521315626 27.890321961649693 33.5921544977316 20.540075019303078 84-85 18.102445899856768 27.41523187733722 33.71124812394204 20.77107409886397 86-87 17.794925917296016 27.48255869735057 33.42128227269197 21.30123311266145 88-89 18.106973322916524 27.06839914655263 33.54418234899053 21.280445181540316 90-91 17.74871896795535 27.376048869973246 33.7424868584274 21.132745303644 92-93 17.303909810506116 27.133376737598375 33.914036710870484 21.648676741025024 94-95 17.091334402426934 27.455374479730626 33.514485304531775 21.938805813310672 96-97 17.320001644759387 27.177821333442985 33.26228886543584 22.239888156361793 98-99 17.537932263325978 26.956921010430516 33.12316963408672 22.38197709215678 100-101 17.537438744388474 26.535074192111306 33.06557920107833 22.861907862421894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 585.0 1 453.5 2 826.5 3 1046.0 4 548.0 5 353.0 6 606.0 7 1344.5 8 1992.5 9 2042.5 10 1434.0 11 837.0 12 693.5 13 659.5 14 652.5 15 595.5 16 579.0 17 611.0 18 608.5 19 633.5 20 714.5 21 821.5 22 961.0 23 1146.0 24 1341.0 25 1582.5 26 1952.0 27 2346.5 28 2759.0 29 3296.0 30 3814.5 31 4322.5 32 4971.5 33 5679.0 34 6465.5 35 7119.5 36 7673.5 37 8154.0 38 8333.0 39 8313.5 40 8248.0 41 8304.0 42 8343.0 43 8282.0 44 8179.0 45 7990.5 46 7719.5 47 7577.0 48 7888.5 49 7412.5 50 6495.5 51 5950.0 52 5108.5 53 4375.0 54 3748.5 55 3206.5 56 2765.0 57 2245.0 58 1727.0 59 1330.0 60 1011.0 61 732.5 62 523.0 63 340.0 64 199.5 65 134.5 66 104.0 67 86.0 68 70.5 69 50.0 70 36.0 71 23.0 72 18.0 73 18.0 74 16.5 75 8.0 76 9.5 77 11.0 78 5.5 79 2.5 80 2.0 81 2.5 82 1.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04660151591990022 2 0.0 3 0.0 4 0.0 5 0.011878817783503977 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0050256536776362975 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2843880352892262E-4 22-23 2.2843880352892262E-4 24-25 0.0011421940176446132 26-27 0.002969704445875994 28-29 0.003655020856462762 30-31 9.137552141156905E-4 32-33 0.0 34-35 0.0 36-37 2.2843880352892262E-4 38-39 0.0 40-41 0.0 42-43 0.00959442974821475 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.2843880352892262E-4 86-87 0.0 88-89 0.0 90-91 0.004797214874107375 92-93 0.0020559492317603037 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.008909113337627983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 218877.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.82191622164456 #Duplication Level Percentage of deduplicated Percentage of total 1 91.90689699068764 74.28091528772673 2 6.243186786163349 10.09172638774745 3 1.0503359940956902 2.546705031581389 4 0.2956495415274611 0.9557984990520032 5 0.13767648669070603 0.5563638736503302 6 0.07702414596654482 0.3735143443411084 7 0.05720838060199806 0.3236583660933449 8 0.03139724799432728 0.20300685975901733 9 0.03250745076178331 0.2364583015843277 >10 0.14190390454207708 2.182245377175202 >50 0.010827138008696188 0.6286687400239849 >100 0.010827138008696188 1.4185345928746327 >500 0.00170954810663624 0.9105334058808925 >1k 0.0028492468443937335 5.291870932509587 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3648 1.6666895105470196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3105 1.4186049699146097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2181 0.9964500609931605 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1523 0.6958245955490984 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1033 0.47195456809075415 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.31478867126285537 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.29925483262288866 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 0.28920352526761606 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.15716589682789878 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.14757146707968402 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0013706328211735357 0.0 24-25 0.0 0.0 0.0 0.0022843880352892264 0.0 26-27 0.0 0.0 0.0 0.004111898463520607 0.0 28-29 0.0 0.0 0.0 0.005482531284694144 0.0 30-31 0.0 0.0 0.0 0.00959442974821475 0.0 32-33 0.0 0.0 0.0 0.014848522229379971 0.0 34-35 0.0 0.0 0.0 0.02010261471054519 0.0 36-37 0.0 0.0 0.0 0.0242145131740658 0.0 38-39 0.0 0.0 0.0 0.031752993690520245 0.0 40-41 0.0 0.0 0.0 0.04797214874107375 0.0 42-43 0.0 0.0 0.0 0.0806388976457097 0.0 44-45 0.0 0.0 0.0 0.10462497201624657 0.0 46-47 0.0 0.0 0.0 0.17087222503963412 0.0 48-49 0.0 0.0 0.0 0.23597728404537707 0.0 50-51 0.0 0.0 0.0 0.2734412478241204 0.0 52-53 0.0 0.0 0.0 0.3385463068298633 0.0 54-55 0.0 0.0 0.0 0.4073063866920691 0.0 56-57 0.0 0.0 0.0 0.5468824956482408 0.0 58-59 0.0 0.0 0.0 0.6839457777655944 0.0 60-61 0.0 0.0 0.0 0.8260347135605842 0.0 62-63 0.0 0.0 0.0 0.974519935854384 0.0 64-65 0.0 0.0 0.0 1.1079281971152748 0.0 66-67 0.0 0.0 0.0 1.3174065799512968 0.0 68-69 0.0 0.0 0.0 1.6379062213023752 0.0 70-71 0.0 0.0 0.0 1.8508111861913312 0.0 72-73 0.0 0.0 0.0 2.0749096524532042 0.0 74-75 0.0 0.0 0.0 2.344695879420862 0.0 76-77 0.0 0.0 0.0 2.6594845506837173 0.0 78-79 0.0 0.0 0.0 2.9815832636594983 0.0 80-81 0.0 0.0 0.0 3.4622185062843513 0.0 82-83 0.0 0.0 0.0 3.9727792321714936 0.0 84-85 0.0 0.0 0.0 4.5475312618502635 0.0 86-87 0.0 0.0 0.0 5.019485829941017 0.0 88-89 0.0 0.0 0.0 5.736098356611247 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACACCG 25 0.0050125644 57.00946 5 GGTATCA 1405 0.0 39.92723 1 TAGCCCT 210 0.0 36.196484 4 GTATCAA 2190 0.0 32.77905 1 CCTAGGA 105 1.8899336E-6 31.671923 2 GTATTAA 105 6.8409325E-5 27.165985 1 GTATAGC 175 4.8694346E-9 27.165983 1 TAGGACT 70 0.0083702095 27.147362 4 TATCAAC 2710 0.0 26.64649 2 ATAGCCC 180 6.6229404E-9 26.39327 3 AGTACAG 90 8.9156744E-4 26.39327 5 GGAGTGT 90 8.927583E-4 26.387241 6 ATCAACG 2765 0.0 26.116451 3 ACAGTAT 110 9.446315E-5 25.907473 6 TCAACGC 2790 0.0 25.882431 4 CAACGCA 2845 0.0 25.382069 5 GTACAGA 95 0.0012173204 25.021301 1 GTGTAGC 190 1.1692464E-8 25.021301 1 GTCTTAC 95 0.0012173204 25.021301 1 TAGACTG 95 0.0012221995 25.004148 5 >>END_MODULE